Rorug01G0320700

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008283
Physical Location & Seq
Forward (+)
43995405 .. 43998434
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0320700.1

Sequence Viewer

Length: 846 bp
ATGTCTCAAGCAAGGCAATTTCAGATGGTGGGGGGTAACAATAATATTCCAGGCCATCAATACAATGACACAACTTTCACCAAAATCTTTGTTGGGGGTTTGGCTTGGGAGACTCAGAGGGAGACTATGAGGAGGTACTTTGAGCAGTTTGGAGAGATCTTGGAGGCTGTTGTTATTACAGATAAAAACACTGGAAGATCCAAAGGCTATGGATTTGTCACATTTAAGGATCCAGAGGCAGCCATGAGGGCTTGTCAGAACCCATCTCCTGTTATTGATGGAAGAAGAGCAAACTGCAATCTTGCTTCTATTGGTGCTCAGAAAACTCGTCCACCCACTCCTCAACATGGTTTTGGAAGGTTTAGACCAGCACCTGGTTTGGTAGCTCCACCTGCTTATCATGGCTCTTCATCCACATACTTCCATCAACCCAGTGGTCAATACACATTTCCTTATTCAGCTTATGGGTACTCTGGATATTCACAAGATTCCATATATCCCATGAACTATTATAGTGTTTATGGTGGTCAACAATTCTCACCTTACTACACCACCACAGCTGGGGCATCAGGGCCAGCTGGCATGTACCACAATTACTACCCATACTATGCTGCTGCTCAATATGCACATGCTAGTAGTAACCAAGCACAAGGCTTTGGAGTTCAATATCCCCAAATGGTGCAGTACCCATATTTGCCTCAACACTATGGTTCTTCAGCTGGGATTCTCTCACTTCCTTCTTCAATCCCAGTTGCTACTACCACTTCTACCACCACAGGTGTAACAATAGCCTCTGGGGTAACTGGAGCTTCTGCAACCAATTCTGAACAGAATTCTTCGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

281

Amino Acids

30.52

Weight (kDa)

8.87

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 29 - 86 1.6e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 374
AclWI GGATC 3 cut(s) 192, 224, 237
AcsI RAATTY 1 cut(s) 832
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 4 cut(s) 137, 470, 587, 686
AfiI CCNNNNNNNGG 3 cut(s) 347, 374, 561
AgsI TTSAA 2 cut(s) 665, 744
AjnI CCWGG 2 cut(s) 49, 373
AluBI AGCT 6 cut(s) 386, 461, 560, 578, 719, 809
AluI AGCT 6 cut(s) 386, 461, 560, 578, 719, 809
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 3 cut(s) 9, 104, 116
AlwI GGATC 3 cut(s) 192, 224, 237
AlwNI CAGNNNCTG 1 cut(s) 374
AoxI GGCC 2 cut(s) 52, 572
ApeKI GCWGC 3 cut(s) 239, 611, 614
ApoI RAATTY 1 cut(s) 832
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 2 cut(s) 70, 531
BamHI GGATCC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 251, 598, 601
BccI CCATC 5 cut(s) 19, 63, 271, 272, 432
BciT130I CCWGG 2 cut(s) 51, 375
BcoDI GTCTC 3 cut(s) 9, 104, 116
BfaI CTAG 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 400
BglI GCCNNNNNGGC 1 cut(s) 248
BglII AGATCT 1 cut(s) 156
BisI GCNGC 3 cut(s) 240, 612, 615
BlsI GCNGC 3 cut(s) 241, 613, 616
Bme1390I CCNGG 2 cut(s) 51, 375
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 2 cut(s) 51, 375
BmrI ACTGGG 2 cut(s) 426, 743
BmsI GCATC 1 cut(s) 575
BmuI ACTGGG 2 cut(s) 426, 743
BpmI CTGGAG 1 cut(s) 825
Bsc4I CCNNNNNNNGG 3 cut(s) 347, 374, 561
Bse1I ACTGG 4 cut(s) 196, 432, 749, 808
BseBI CCWGG 2 cut(s) 51, 375
BseGI GGATG 1 cut(s) 410
BseLI CCNNNNNNNGG 3 cut(s) 347, 374, 561
BseMII CTCAG 2 cut(s) 128, 332
BseNI ACTGG 4 cut(s) 196, 432, 749, 808
BseRI GAGGAG 2 cut(s) 145, 330
BseXI GCAGC 3 cut(s) 251, 598, 601
BseYI CCCAGC 2 cut(s) 560, 719
BsgI GTGCAG 1 cut(s) 701
BshFI GGCC 2 cut(s) 54, 574
BsiHKAI GWGCWC 1 cut(s) 319
BslI CCNNNNNNNGG 3 cut(s) 347, 374, 561
BsmAI GTCTC 3 cut(s) 9, 104, 116
BsnI GGCC 2 cut(s) 54, 574
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 3 cut(s) 156, 197, 229
BspANI GGCC 2 cut(s) 54, 574
BspCNI CTCAG 2 cut(s) 127, 331
BspLI GGNNCC 1 cut(s) 231
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 3 cut(s) 192, 224, 237
BspQI GCTCTTC 2 cut(s) 280, 412
BsrI ACTGG 4 cut(s) 196, 432, 749, 808
BssMI GATC 3 cut(s) 156, 197, 229
Bst2UI CCWGG 2 cut(s) 51, 375
Bst6I CTCTTC 2 cut(s) 280, 412
BstC8I GCNNGC 2 cut(s) 576, 580
BstDEI CTNAG 2 cut(s) 114, 318
BstF5I GGATG 1 cut(s) 410
BstKTI GATC 3 cut(s) 159, 200, 232
BstMAI GTCTC 3 cut(s) 9, 104, 116
BstMBI GATC 3 cut(s) 156, 197, 229
BstMWI GCNNNNNNNGC 3 cut(s) 248, 392, 623
BstNI CCWGG 2 cut(s) 51, 375
BstNSI RCATGY 2 cut(s) 586, 632
BstSCI CCNGG 2 cut(s) 49, 373
BstV1I GCAGC 3 cut(s) 251, 598, 601
BstX2I RGATCY 3 cut(s) 156, 197, 229
BstYI RGATCY 3 cut(s) 156, 197, 229
BsuRI GGCC 2 cut(s) 54, 574
BtsCI GGATG 1 cut(s) 410
BtsIMutI CAGTG 2 cut(s) 189, 439
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 576, 580
CaiI CAGNNNCTG 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 572
CsiI ACCWGGT 1 cut(s) 373
Csp6I GTAC 4 cut(s) 136, 469, 586, 685
CviAII CATG 6 cut(s) 244, 347, 401, 502, 583, 629
CviQI GTAC 4 cut(s) 136, 469, 586, 685
DdeI CTNAG 2 cut(s) 114, 318
DpnI GATC 3 cut(s) 158, 199, 231
DpnII GATC 3 cut(s) 156, 197, 229
Eam1104I CTCTTC 2 cut(s) 280, 412
EarI CTCTTC 2 cut(s) 280, 412
Eco57I CTGAAG 1 cut(s) 699
EcoRI GAATTC 1 cut(s) 832
EcoRII CCWGG 2 cut(s) 49, 373
FaeI CATG 6 cut(s) 247, 350, 404, 505, 586, 632
FatI CATG 6 cut(s) 243, 346, 400, 501, 582, 628
Fnu4HI GCNGC 3 cut(s) 240, 612, 615
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 3 cut(s) 240, 612, 615
FspBI CTAG 1 cut(s) 633
GluI GCNGC 3 cut(s) 240, 612, 615
GsaI CCCAGC 2 cut(s) 564, 723
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 2 cut(s) 54, 574
Hin1II CATG 6 cut(s) 247, 350, 404, 505, 586, 632
HincII GTYRAC 1 cut(s) 530
HindII GTYRAC 1 cut(s) 530
HinfI GANTC 3 cut(s) 112, 488, 724
HphI GGTGA 2 cut(s) 70, 531
Hpy166II GTNNAC 2 cut(s) 332, 530
Hpy188I TCNGA 5 cut(s) 24, 117, 258, 321, 826
Hpy188III TCNNGA 2 cut(s) 233, 474
Hpy8I GTNNAC 2 cut(s) 332, 530
HpyAV CCTTC 2 cut(s) 351, 747
HpyCH4V TGCA 4 cut(s) 297, 626, 682, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 392, 623
HpyF3I CTNAG 2 cut(s) 114, 318
Hsp92II CATG 6 cut(s) 247, 350, 404, 505, 586, 632
Kzo9I GATC 3 cut(s) 156, 197, 229
LguI GCTCTTC 2 cut(s) 280, 412
LmnI GCTCC 2 cut(s) 391, 806
Lsp1109I GCAGC 3 cut(s) 251, 598, 601
LweI GCATC 1 cut(s) 575
MabI ACCWGGT 1 cut(s) 373
MaeI CTAG 1 cut(s) 633
MaeIII GTNAC 5 cut(s) 35, 217, 638, 781, 799
MalI GATC 3 cut(s) 158, 199, 231
MboI GATC 3 cut(s) 156, 197, 229
MboII GAAGA 7 cut(s) 207, 294, 297, 399, 705, 732, 828
MflI RGATCY 3 cut(s) 156, 197, 229
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 5 cut(s) 17, 533, 592, 820, 832
MlyI GAGTC 1 cut(s) 106
MnlI CCTC 9 cut(s) 111, 123, 126, 157, 229, 240, 351, 708, 802
MseI TTAA 2 cut(s) 225, 844
MspA1I CMGCKG 3 cut(s) 560, 578, 719
MspR9I CCNGG 2 cut(s) 51, 375
MvaI CCWGG 2 cut(s) 51, 375
MwoI GCNNNNNNNGC 3 cut(s) 248, 392, 623
NdeII GATC 3 cut(s) 156, 197, 229
NlaIII CATG 6 cut(s) 247, 350, 404, 505, 586, 632
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 2 cut(s) 586, 632
PaqCI CACCTGC 1 cut(s) 400
PciSI GCTCTTC 2 cut(s) 280, 412
PfeI GAWTC 2 cut(s) 488, 724
PflMI CCANNNNNTGG 1 cut(s) 374
PkrI GCNGC 3 cut(s) 241, 613, 616
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Psp6I CCWGG 2 cut(s) 49, 373
PspFI CCCAGC 2 cut(s) 560, 719
PspGI CCWGG 2 cut(s) 49, 373
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 572
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 3 cut(s) 156, 197, 229
PvuII CAGCTG 3 cut(s) 560, 578, 719
RsaI GTAC 4 cut(s) 137, 470, 587, 686
RsaNI GTAC 4 cut(s) 136, 469, 586, 685
SapI GCTCTTC 2 cut(s) 280, 412
SaqAI TTAA 2 cut(s) 225, 844
SatI GCNGC 3 cut(s) 240, 612, 615
Sau3AI GATC 3 cut(s) 156, 197, 229
Sau96I GGNCC 1 cut(s) 572
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 2 cut(s) 51, 375
SduI GDGCHC 1 cut(s) 319
SexAI ACCWGGT 1 cut(s) 373
SfaNI GCATC 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 5 cut(s) 17, 533, 592, 820, 832
SspI AATATT 1 cut(s) 46
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 2 cut(s) 49, 373
TaqI TCGA 1 cut(s) 839
TasI AATT 5 cut(s) 17, 533, 592, 820, 832
TfiI GAWTC 2 cut(s) 488, 724
Tru1I TTAA 2 cut(s) 225, 844
Tru9I TTAA 2 cut(s) 225, 844
TscAI CASTG 2 cut(s) 196, 439
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 3 cut(s) 239, 611, 614
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 399, 518
TspRI CASTG 2 cut(s) 196, 439
Van91I CCANNNNNTGG 1 cut(s) 374
XapI RAATTY 1 cut(s) 832
XceI RCATGY 2 cut(s) 586, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.