Prupe.2G226100_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0008283
Physical Location & Seq
Forward (+)
25272576 .. 25274970
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G226100.1

Sequence Viewer

Length: 1002 bp
ATGGCCTCGCTACCGTACGCCGACGTGGATTTCAGCTTGAGAGCATTGTCCGGTCGGGCCGAGGGCTTCGGCCGGTTCGCCATCGGCGGCCTCCACGGGCCGCTCTATTTCGTCACCACTTTGGCAGATGATGGTCCAGGCTCACTTCGTGAAGGATGTCGAAAACAAGAACCACTGTGGATTGTCTTTGAGGTTTCTGGCACCATTCACCTTTCGTCTTACTTGAGTGTGTCATCTTATAAGACAATTGATGGACGAGGCCAGAGAGTAAAGCTCACAGGAAAGGGCCTTAGACTGAAGGAATGTGAACACATAATTGTCTGCAACCTTGAGTTTCAAGGCGGTAGAGGACATGATGTTGATGGCATTCAGATAAAACCGAATTCTAGGCACATTTGGATAGACCGATGCAGCCTTCGTGATTATGATGATGGACTCATAGATATCACCAGACAAAGTACAGATATAACCATCTCTAGATGTTACTTCGCACAACATGACAAGACAATGCTTATTGGAGCAGACCCCTCCCACGTTGGCGATAGATGCATTCGGGTGACAATTCATCATTGTTTTTTTGATGGGACAAGGCAAAGACAACCTCGCCTTAGATTTGGGAAAGTTCATCTTTACAACAATTACACTCGAAACTGGGGTATATATGCTGTTTGCGCCAGCGTAGAATCCCAGATATACTCCCAATGCAACATATATGAAGCAGGAAATAAGAAAAAGACTTTTGAATATTATACAGAAAAGGCAGCAGACAGAGAAGAGGCAAAATCTGGCTTCATAAGTTCTGAGGGCGACCTATTCCTGAACGGAGCTCAACCATGCGCATTAACAGGGTTCAATCAAGAATGCATGTTCCATCCAAGTGAATTTTATCCACATTGGACTATGGAAGCAGCCTCAGATTCTCTTAAAACTATTCTCCAAATTCTTACAGGTTGGCAATCCATTCATAGGCCAGCAGAGCAGGCGCAGGCGGTAACTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

334

Amino Acids

37.52

Weight (kDa)

6.88

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
Acc16I TGCGCA 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 103
AciI CCGC 4 cut(s) 87, 101, 342, 989
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 3 cut(s) 382, 881, 939
AcuI CTGAAG 1 cut(s) 317
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 2 cut(s) 17, 460
AfiI CCNNNNNNNGG 1 cut(s) 966
AgsI TTSAA 3 cut(s) 338, 743, 853
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 3 cut(s) 36, 274, 827
AluI AGCT 3 cut(s) 36, 274, 827
Alw21I GWGCWC 1 cut(s) 829
AoxI GGCC 8 cut(s) 3, 57, 70, 88, 98, 259, 286, 968
ApeKI GCWGC 3 cut(s) 411, 761, 908
ApoI RAATTY 3 cut(s) 382, 881, 939
AspLEI GCGC 3 cut(s) 674, 839, 985
AspS9I GGNCC 4 cut(s) 57, 98, 134, 286
AsuHPI GGTGA 4 cut(s) 106, 200, 439, 568
AvaII GGWCC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 829
Bbv12I GWGCWC 1 cut(s) 829
BbvI GCAGC 3 cut(s) 423, 773, 920
BccI CCATC 8 cut(s) 89, 125, 245, 356, 425, 479, 575, 879
BciT130I CCWGG 1 cut(s) 138
BfaI CTAG 2 cut(s) 387, 477
BisI GCNGC 5 cut(s) 88, 101, 412, 762, 909
BlsI GCNGC 5 cut(s) 89, 102, 413, 763, 910
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 57, 98, 134, 286
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 661
BmsI GCATC 2 cut(s) 398, 536
BmuI ACTGGG 1 cut(s) 661
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpuEI CTTGAG 3 cut(s) 58, 244, 350
BsaJI CCNNGG 2 cut(s) 60, 94
BsaWI WCCGGW 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 966
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 656
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 60, 94
BseGI GGATG 2 cut(s) 161, 871
BseLI CCNNNNNNNGG 1 cut(s) 966
BseMII CTCAG 2 cut(s) 792, 927
BseNI ACTGG 1 cut(s) 656
BseX3I CGGCCG 1 cut(s) 70
BseXI GCAGC 3 cut(s) 423, 773, 920
Bsh1285I CGRYCG 2 cut(s) 55, 73
BshFI GGCC 8 cut(s) 5, 59, 72, 90, 100, 261, 288, 970
BshNI GGYRCC 1 cut(s) 200
BsiEI CGRYCG 2 cut(s) 55, 73
BsiHKAI GWGCWC 1 cut(s) 829
BsiSI CCGG 2 cut(s) 51, 73
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 1 cut(s) 966
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 3 cut(s) 366, 549, 866
BsnI GGCC 8 cut(s) 5, 59, 72, 90, 100, 261, 288, 970
Bsp1286I GDGCHC 1 cut(s) 829
BspACI CCGC 4 cut(s) 87, 101, 342, 989
BspANI GGCC 8 cut(s) 5, 59, 72, 90, 100, 261, 288, 970
BspCNI CTCAG 2 cut(s) 793, 926
BspLI GGNNCC 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 72
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 60, 94
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 15, 177, 997
Bst6I CTCTTC 1 cut(s) 768
BstC8I GCNNGC 4 cut(s) 676, 972, 981, 987
BstDEI CTNAG 4 cut(s) 290, 608, 801, 913
BstDSI CCRYGG 1 cut(s) 94
BstF5I GGATG 2 cut(s) 161, 871
BstHHI GCGC 3 cut(s) 674, 839, 985
BstMCI CGRYCG 2 cut(s) 55, 73
BstMWI GCNNNNNNNGC 4 cut(s) 546, 671, 976, 980
BstNI CCWGG 1 cut(s) 138
BstNSI RCATGY 1 cut(s) 868
BstSCI CCNGG 1 cut(s) 136
BstV1I GCAGC 3 cut(s) 423, 773, 920
BstZI CGGCCG 1 cut(s) 70
BsuRI GGCC 8 cut(s) 5, 59, 72, 90, 100, 261, 288, 970
BtgI CCRYGG 1 cut(s) 94
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 161, 871
BtsIMutI CAGTG 1 cut(s) 173
Cac8I GCNNGC 4 cut(s) 676, 972, 981, 987
CfoI GCGC 3 cut(s) 674, 839, 985
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 4 cut(s) 57, 98, 134, 286
Csp6I GTAC 2 cut(s) 16, 459
CviAII CATG 4 cut(s) 353, 497, 834, 865
CviQI GTAC 2 cut(s) 16, 459
DdeI CTNAG 4 cut(s) 290, 608, 801, 913
DraIII CACNNNGTG 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 70
EagI CGGCCG 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Ecl136II GAGCTC 1 cut(s) 827
EclXI CGGCCG 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 829
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 827
Eco57I CTGAAG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 827
EcoO109I RGGNCCY 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 136
EcoRV GATATC 1 cut(s) 445
EcoT22I ATGCAT 2 cut(s) 551, 866
EcoT38I GRGCYC 1 cut(s) 829
FaeI CATG 4 cut(s) 356, 500, 837, 868
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 1 cut(s) 598
FatI CATG 4 cut(s) 352, 496, 833, 864
Fnu4HI GCNGC 5 cut(s) 88, 101, 412, 762, 909
FokI GGATG 2 cut(s) 168, 858
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 5 cut(s) 88, 101, 412, 762, 909
FspAI RTGCGCAY 1 cut(s) 838
FspBI CTAG 2 cut(s) 387, 477
FspI TGCGCA 1 cut(s) 838
GlaI GCGC 3 cut(s) 673, 838, 984
GluI GCNGC 5 cut(s) 88, 101, 412, 762, 909
HaeIII GGCC 8 cut(s) 5, 59, 72, 90, 100, 261, 288, 970
HapII CCGG 2 cut(s) 51, 73
HhaI GCGC 3 cut(s) 674, 839, 985
Hin1II CATG 4 cut(s) 356, 500, 837, 868
Hin6I GCGC 3 cut(s) 672, 837, 983
HinP1I GCGC 3 cut(s) 672, 837, 983
HinfI GANTC 3 cut(s) 435, 683, 917
HpaII CCGG 2 cut(s) 51, 73
HphI GGTGA 4 cut(s) 106, 200, 439, 568
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 3 cut(s) 372, 802, 916
Hpy188III TCNNGA 5 cut(s) 149, 419, 477, 817, 857
Hpy8I GTNNAC 1 cut(s) 308
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 146, 292, 425
HpyCH4III ACNGT 3 cut(s) 15, 177, 997
HpyCH4IV ACGT 2 cut(s) 24, 534
HpyCH4V TGCA 5 cut(s) 324, 411, 549, 705, 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 546, 671, 976, 980
HpyF3I CTNAG 4 cut(s) 290, 608, 801, 913
HpySE526I ACGT 2 cut(s) 24, 534
Hsp92II CATG 4 cut(s) 356, 500, 837, 868
HspAI GCGC 3 cut(s) 672, 837, 983
LmnI GCTCC 2 cut(s) 518, 824
Lsp1109I GCAGC 3 cut(s) 423, 773, 920
LweI GCATC 2 cut(s) 398, 536
MaeI CTAG 2 cut(s) 387, 477
MaeII ACGT 2 cut(s) 24, 534
MaeIII GTNAC 4 cut(s) 112, 482, 556, 991
MbiI CCGCTC 1 cut(s) 103
MboII GAAGA 1 cut(s) 785
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 829
MluCI AATT 7 cut(s) 246, 315, 382, 561, 637, 881, 939
MlyI GAGTC 1 cut(s) 429
Mph1103I ATGCAT 2 cut(s) 551, 866
MseI TTAA 2 cut(s) 842, 924
MslI CAYNNNNRTG 2 cut(s) 554, 876
MspI CCGG 2 cut(s) 51, 73
MspR9I CCNGG 1 cut(s) 138
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 3 cut(s) 366, 549, 866
MvaI CCWGG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 546, 671, 976, 980
NlaIII CATG 4 cut(s) 356, 500, 837, 868
NlaIV GGNNCC 1 cut(s) 202
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 112, 556
NsbI TGCGCA 1 cut(s) 838
NsiI ATGCAT 2 cut(s) 551, 866
NspI RCATGY 1 cut(s) 868
PctI GAATGC 3 cut(s) 366, 549, 866
PfeI GAWTC 2 cut(s) 683, 917
Pfl23II CGTACG 1 cut(s) 15
PkrI GCNGC 5 cut(s) 89, 102, 413, 763, 910
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PsiI TTATAA 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 829
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 4 cut(s) 57, 98, 134, 286
RsaI GTAC 2 cut(s) 17, 460
RsaNI GTAC 2 cut(s) 16, 459
RseI CAYNNNNRTG 2 cut(s) 554, 876
SacI GAGCTC 1 cut(s) 829
SaqAI TTAA 2 cut(s) 842, 924
SatI GCNGC 5 cut(s) 88, 101, 412, 762, 909
Sau96I GGNCC 4 cut(s) 57, 98, 134, 286
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 829
SfaNI GCATC 2 cut(s) 398, 536
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 554, 876
SmlI CTYRAG 3 cut(s) 37, 223, 329
SmoI CTYRAG 3 cut(s) 37, 223, 329
Sse9I AATT 7 cut(s) 246, 315, 382, 561, 637, 881, 939
SsiI CCGC 4 cut(s) 87, 101, 342, 989
SspI AATATT 1 cut(s) 746
SspMI CTAG 2 cut(s) 387, 477
SstI GAGCTC 1 cut(s) 829
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 15, 177, 997
TaiI ACGT 2 cut(s) 27, 537
TaqI TCGA 2 cut(s) 160, 646
TaqII GACCGA 1 cut(s) 420
TasI AATT 7 cut(s) 246, 315, 382, 561, 637, 881, 939
TatI WGTACW 1 cut(s) 458
TauI GCSGC 2 cut(s) 90, 103
TfiI GAWTC 2 cut(s) 683, 917
Tru1I TTAA 2 cut(s) 842, 924
Tru9I TTAA 2 cut(s) 842, 924
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 2 cut(s) 112, 556
TseI GCWGC 3 cut(s) 411, 761, 908
Tsp45I GTSAC 2 cut(s) 112, 556
TspDTI ATGAA 5 cut(s) 554, 614, 729, 781, 953
TspGWI ACGGA 1 cut(s) 837
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 3 cut(s) 382, 881, 939
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 1 cut(s) 868
XspI CTAG 2 cut(s) 387, 477
Zsp2I ATGCAT 2 cut(s) 551, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.