Rorug01G0320900

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008283
Physical Location & Seq
Reverse (-)
44002199 .. 44006238
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0320900.1

Sequence Viewer

Length: 1821 bp
ATGGCAACTGTCACTGCTTCAAGCTTCACTTCCACAAGCGCTCATGTCAACTACGGCGTGTCATCTACCCCAGAGGCTAAACCGACGTTTCATAACTTGGGTTTTGGGAAGCAGGCCCCGACTCATAATGGGTTGAGAGCTTTGAACGTAGTGAAGGCGAAATCGATTGCTATGAGGAAATTGAACACTCGGAGTTCGAAGCGTTCGGGTGCTATTGTATGTGGGAGTGGGATGAATTTGGTGTTTGTGGGTGCAGAGGTTGGTCCATGGAGCAAGACTGGTGGACTTGGAGATGTTCTTGGAGGTCTCCCACCAGCTATGGCCGCAAATGGGCACCGTGTGATGACTGTTTCACCGCGTTATGATCAGTACAAAGATGCATGGGATACAGAAGTTACGGTTGAGCTTAAAGTAGGACAGAAGATTGAGACGGTTCGGTTTTTTCACTGCCACAAACGAGGGGTTGATCGTGTTTTTGTAGATCACCCACGGTTTCTCGAAAAGGTATGGGGGAAAACTGCATCGAAAATATACGGGCCTAGCGCTGGTGAGGATTACACGGACAACCAACTTAGATTCAGCTTGTTATGCCAGGCAGCTCTAGAGGCACCGAGAGTTCTGAATCTTACCAGCAACCAATATTTTTCAGGACCATATGGAGAAGATGTTGTTTTCATTGCCAATGACTGGCACACAGCCCTCCTCTCATGCTACCTGAAAGCCATATACCAACCCAAAGGAATATACAAGAGTGCCAAAGTAGCCTTTTGCATTCACAACATTGCATACCAAGGCAGATTTGCATTCGCGGACTTCTCACTTCTCAATCTGCCATCTGCATTTAAAAGCTCCTTTGACTTCATTGATGGCTATAACAAACCAGTGAAAGGGAGGAAAATTAACTGGATGAAAGCTGGAATCTTAGAATCTGATAAGGTCTTGACCGTTAGCCCGTACTATGCCAAAGAACTTGTTTCTTCAATTGAGAAAGGAGTCGAATTGGATAACATTATTCGGAAAACTGAAATTCTTGGTATAGTAAATGGCATGGATGTCCAGGAATGGAACCCGTTAACTGACAAGTATACAGATGTCAAATATGATGCTACAACAGTGATGGATGCAAAGCCTATTTTGAAAGAAGCCCTCCAAGCAGAAGTCGGTTTGCCAGTGGATAAGAACATCCCTGTAATAGGTTTCATTGGTAGGCTTGAAGAGCAGAAGGGTTCGGATATTCTTATAGAAGCGATTCCCCACTTCATCAAAGAGAATGTTCAGATTATAGTTCTTGGGACTGGCAAAAAACCAATGGAGAAGCAGCTTCTCCAATTGGAAGAAGAATACCCTGACAAAGCTCGAGGAGTAGCAAAATTTAACGTTCCTCTGGCCCATATGATAACAGCCGGAGCTGATTTTATGTTGATTCCCAGCAGATTTGAGCCTTGTGGTCTCATTCAGTTGCATGCTATGCGTTATGGAACTGTTCCCATTGTTGCATCAACTGGCGGGTTGGTAGACACTGTTAAAGAAGGATTCACAGGATTTCAGATGGGAAGTTTCAATGTCGAGTGTGACGTTGTTGATCCTGTGGATGTACAAGCTGTGGCCACAACTGTTACAAGAGCTCTTAGAACTTATGGAACTCCGGCTTTTGCTGAGATCATTAAGAACTGCATGTCTCAAGATTTCTCATGGAAGGGACCTGCTAAGAAGTGGGAAGAAGTGCTGCTAAGTTTGGGTGTTGCTGACAGCGAACCCGGACTAGATGGTGATGGTGAGGAAATTGCACCCCTCTCCAAGGAAAATGTCGCAACGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

606

Amino Acids

66.37

Weight (kDa)

6.87

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 80 - 339 4.3e-64 Starch synthase catalytic domain
GT4-conflict PF20706 369 - 570 7e-09 Family 4 Glycosyltransferase in conflict systems
Glycos_transf_1 PF00534 391 - 517 5.4e-17 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 396 - 543 9.1e-16 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1711
AccB1I GGYRCC 2 cut(s) 333, 607
AccB7I CCANNNNNTGG 1 cut(s) 687
AccI GTMKAC 2 cut(s) 1085, 1515
AccII CGCG 2 cut(s) 358, 809
AciI CCGC 4 cut(s) 324, 356, 809, 1506
AclI AACGTT 1 cut(s) 1377
AclWI GGATC 1 cut(s) 1577
AcoI YGGCCR 2 cut(s) 321, 1605
AcsI RAATTY 3 cut(s) 235, 1026, 1370
AdeI CACNNNGTG 1 cut(s) 340
AfaI GTAC 3 cut(s) 371, 956, 1596
AfeI AGCGCT 2 cut(s) 40, 544
AfiI CCNNNNNNNGG 6 cut(s) 330, 545, 687, 887, 1193, 1798
AgsI TTSAA 7 cut(s) 21, 145, 184, 981, 1138, 1214, 1561
AjnI CCWGG 2 cut(s) 591, 1056
Alw21I GWGCWC 1 cut(s) 1627
Alw26I GTCTC 4 cut(s) 311, 422, 1454, 1683
AlwI GGATC 1 cut(s) 1577
Ama87I CYCGRG 1 cut(s) 1356
Aor51HI AGCGCT 2 cut(s) 40, 544
AoxI GGCC 5 cut(s) 114, 321, 536, 1386, 1605
ApeKI GCWGC 3 cut(s) 596, 1318, 1726
ApoI RAATTY 3 cut(s) 235, 1026, 1370
Asp700I GAANNNNTTC 1 cut(s) 1248
AspLEI GCGC 2 cut(s) 41, 545
AspS9I GGNCC 6 cut(s) 115, 263, 536, 650, 1387, 1700
AsuC2I CCSGG 1 cut(s) 1758
AsuHPI GGTGA 5 cut(s) 345, 476, 560, 1781, 1787
AsuII TTCGAA 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 1356
AvaII GGWCC 3 cut(s) 263, 650, 1700
BaeGI GKGCMC 1 cut(s) 336
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BalI TGGCCA 1 cut(s) 1607
BanI GGYRCC 2 cut(s) 333, 607
BanII GRGCYC 1 cut(s) 1627
Bbv12I GWGCWC 1 cut(s) 1627
BbvI GCAGC 3 cut(s) 608, 1330, 1713
BccI CCATC 6 cut(s) 841, 860, 1111, 1543, 1760, 1766
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 593, 1058
BciVI GTATCC 1 cut(s) 379
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 1758
BcoDI GTCTC 4 cut(s) 311, 422, 1454, 1683
BfaI CTAG 3 cut(s) 540, 602, 1763
BfoI RGCGCY 2 cut(s) 42, 546
BfuAI ACCTGC 1 cut(s) 1711
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 4 cut(s) 324, 597, 1319, 1727
BlsI GCNGC 4 cut(s) 325, 598, 1320, 1728
Bme1390I CCNGG 3 cut(s) 593, 1058, 1758
Bme18I GGWCC 3 cut(s) 263, 650, 1700
BmeT110I CYCGRG 1 cut(s) 1356
BmgT120I GGNCC 6 cut(s) 115, 263, 536, 650, 1387, 1700
BmiI GGNNCC 5 cut(s) 117, 335, 609, 1067, 1701
BmrFI CCNGG 3 cut(s) 593, 1058, 1758
BmsI GCATC 5 cut(s) 367, 530, 1093, 1111, 1505
Bpu14I TTCGAA 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 1665
BpuMI CCSGG 1 cut(s) 1758
Bsa29I ATCGAT 1 cut(s) 164
BsaI GGTCTC 2 cut(s) 311, 1454
BsaJI CCNNGG 4 cut(s) 266, 488, 790, 1797
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 6 cut(s) 330, 545, 687, 887, 1193, 1798
Bse1I ACTGG 7 cut(s) 283, 692, 881, 908, 1169, 1300, 1507
Bse3DI GCAATG 2 cut(s) 675, 780
BseBI CCWGG 2 cut(s) 593, 1058
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 4 cut(s) 266, 488, 790, 1797
BseGI GGATG 6 cut(s) 237, 912, 1057, 1126, 1182, 1597
BseLI CCNNNNNNNGG 6 cut(s) 330, 545, 687, 887, 1193, 1798
BseMI GCAATG 2 cut(s) 675, 780
BseMII CTCAG 1 cut(s) 1647
BseNI ACTGG 7 cut(s) 283, 692, 881, 908, 1169, 1300, 1507
BseRI GAGGAG 2 cut(s) 692, 1374
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 3 cut(s) 608, 1330, 1713
BseYI CCCAGC 1 cut(s) 1427
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 2 cut(s) 358, 809
BshFI GGCC 5 cut(s) 116, 323, 538, 1388, 1607
BshNI GGYRCC 2 cut(s) 333, 607
BshVI ATCGAT 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 1627
BsiHKCI CYCGRG 1 cut(s) 1356
BsiSI CCGG 3 cut(s) 1404, 1646, 1758
BslFI GGGAC 2 cut(s) 1306, 1713
BslI CCNNNNNNNGG 6 cut(s) 330, 545, 687, 887, 1193, 1798
BsmAI GTCTC 4 cut(s) 311, 422, 1454, 1683
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 2 cut(s) 1306, 1713
BsmI GAATGC 2 cut(s) 771, 803
BsnI GGCC 5 cut(s) 116, 323, 538, 1388, 1607
Bso31I GGTCTC 2 cut(s) 311, 1454
BsoBI CYCGRG 1 cut(s) 1356
Bsp119I TTCGAA 1 cut(s) 197
Bsp1286I GDGCHC 2 cut(s) 336, 1627
Bsp1407I TGTACA 1 cut(s) 1594
Bsp143I GATC 5 cut(s) 364, 466, 481, 1582, 1659
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 4 cut(s) 324, 356, 809, 1506
BspANI GGCC 5 cut(s) 116, 323, 538, 1388, 1607
BspCNI CTCAG 1 cut(s) 1648
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 2 cut(s) 358, 809
BspLI GGNNCC 5 cut(s) 117, 335, 609, 1067, 1701
BspMI ACCTGC 1 cut(s) 1711
BspPI GGATC 1 cut(s) 1577
BspQI GCTCTTC 1 cut(s) 1209
BspT104I TTCGAA 1 cut(s) 197
BspT107I GGYRCC 2 cut(s) 333, 607
BspTNI GGTCTC 2 cut(s) 311, 1454
BsrDI GCAATG 2 cut(s) 675, 780
BsrGI TGTACA 1 cut(s) 1594
BsrI ACTGG 7 cut(s) 283, 692, 881, 908, 1169, 1300, 1507
BssECI CCNNGG 4 cut(s) 266, 488, 790, 1797
BssMI GATC 5 cut(s) 364, 466, 481, 1582, 1659
BssNAI GTATAC 1 cut(s) 1086
BssT1I CCWWGG 3 cut(s) 266, 790, 1797
Bst1107I GTATAC 1 cut(s) 1086
Bst2UI CCWGG 2 cut(s) 593, 1058
Bst6I CTCTTC 1 cut(s) 1209
BstAPI GCANNNNNTGC 1 cut(s) 1468
BstAUI TGTACA 1 cut(s) 1594
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 114, 1464
BstDEI CTNAG 6 cut(s) 572, 922, 1628, 1656, 1707, 1730
BstDSI CCRYGG 2 cut(s) 266, 488
BstENI CCTNNNNNAGG 2 cut(s) 1191, 1796
BstF5I GGATG 6 cut(s) 237, 912, 1057, 1126, 1182, 1597
BstFNI CGCG 2 cut(s) 358, 809
BstH2I RGCGCY 2 cut(s) 42, 546
BstHHI GCGC 2 cut(s) 41, 545
BstKTI GATC 5 cut(s) 367, 469, 484, 1585, 1662
BstMAI GTCTC 4 cut(s) 311, 422, 1454, 1683
BstMBI GATC 5 cut(s) 364, 466, 481, 1582, 1659
BstMWI GCNNNNNNNGC 7 cut(s) 323, 588, 605, 761, 1151, 1216, 1468
BstNI CCWGG 2 cut(s) 593, 1058
BstNSI RCATGY 2 cut(s) 1466, 1678
BstSCI CCNGG 3 cut(s) 591, 1056, 1756
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 2 cut(s) 358, 809
BstV1I GCAGC 3 cut(s) 608, 1330, 1713
BstZ17I GTATAC 1 cut(s) 1086
Bsu15I ATCGAT 1 cut(s) 164
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 5 cut(s) 116, 323, 538, 1388, 1607
BsuTUI ATCGAT 1 cut(s) 164
BtgI CCRYGG 2 cut(s) 266, 488
BtsCI GGATG 6 cut(s) 237, 912, 1057, 1126, 1182, 1597
BtsI GCAGTG 2 cut(s) 12, 445
BtsIMutI CAGTG 6 cut(s) 12, 445, 888, 1119, 1176, 1518
BveI ACCTGC 1 cut(s) 1711
Cac8I GCNNGC 2 cut(s) 114, 1464
CfoI GCGC 2 cut(s) 41, 545
Cfr13I GGNCC 6 cut(s) 115, 263, 536, 650, 1387, 1700
ClaI ATCGAT 1 cut(s) 164
Csp6I GTAC 3 cut(s) 370, 955, 1595
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 9 cut(s) 44, 267, 381, 708, 1048, 1463, 1675, 1692, 1818
CviQI GTAC 3 cut(s) 370, 955, 1595
DdeI CTNAG 6 cut(s) 572, 922, 1628, 1656, 1707, 1730
DpnI GATC 5 cut(s) 366, 468, 483, 1584, 1661
DpnII GATC 5 cut(s) 364, 466, 481, 1582, 1659
DraI TTTAAA 1 cut(s) 844
DraIII CACNNNGTG 1 cut(s) 340
EaeI YGGCCR 2 cut(s) 321, 1605
Eam1104I CTCTTC 1 cut(s) 1209
EarI CTCTTC 1 cut(s) 1209
Ecl136II GAGCTC 1 cut(s) 1625
Eco130I CCWWGG 3 cut(s) 266, 790, 1797
Eco24I GRGCYC 1 cut(s) 1627
Eco31I GGTCTC 2 cut(s) 311, 1454
Eco47I GGWCC 3 cut(s) 263, 650, 1700
Eco47III AGCGCT 2 cut(s) 40, 544
Eco53kI GAGCTC 1 cut(s) 1625
Eco88I CYCGRG 1 cut(s) 1356
EcoICRI GAGCTC 1 cut(s) 1625
EcoNI CCTNNNNNAGG 2 cut(s) 1191, 1796
EcoO109I RGGNCCY 2 cut(s) 115, 1700
EcoRII CCWGG 2 cut(s) 591, 1056
EcoT14I CCWWGG 3 cut(s) 266, 790, 1797
EcoT22I ATGCAT 1 cut(s) 382
EcoT38I GRGCYC 1 cut(s) 1627
ErhI CCWWGG 3 cut(s) 266, 790, 1797
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 9 cut(s) 47, 270, 384, 711, 1051, 1466, 1678, 1695, 1821
FalI AAGNNNNNCTT 2 cut(s) 13, 45
FaqI GGGAC 2 cut(s) 1306, 1713
FatI CATG 9 cut(s) 43, 266, 380, 707, 1047, 1462, 1674, 1691, 1817
FauI CCCGC 1 cut(s) 1499
FauNDI CATATG 2 cut(s) 655, 1392
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 2 cut(s) 1085, 1515
Fnu4HI GCNGC 4 cut(s) 324, 597, 1319, 1727
FokI GGATG 6 cut(s) 244, 919, 1064, 1133, 1169, 1604
FriOI GRGCYC 1 cut(s) 1627
Fsp4HI GCNGC 4 cut(s) 324, 597, 1319, 1727
FspBI CTAG 3 cut(s) 540, 602, 1763
GlaI GCGC 2 cut(s) 40, 544
GluI GCNGC 4 cut(s) 324, 597, 1319, 1727
GsaI CCCAGC 1 cut(s) 1431
HaeII RGCGCY 2 cut(s) 42, 546
HaeIII GGCC 5 cut(s) 116, 323, 538, 1388, 1607
HapII CCGG 3 cut(s) 1404, 1646, 1758
HhaI GCGC 2 cut(s) 41, 545
Hin1II CATG 9 cut(s) 47, 270, 384, 711, 1051, 1466, 1678, 1695, 1821
Hin6I GCGC 2 cut(s) 39, 543
HinP1I GCGC 2 cut(s) 39, 543
HincII GTYRAC 2 cut(s) 49, 1074
HindII GTYRAC 2 cut(s) 49, 1074
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 9 cut(s) 121, 576, 622, 918, 926, 993, 1249, 1423, 1533
HpaI GTTAAC 1 cut(s) 1074
HpaII CCGG 3 cut(s) 1404, 1646, 1758
HphI GGTGA 5 cut(s) 345, 476, 560, 1781, 1787
Hpy166II GTNNAC 5 cut(s) 49, 284, 1074, 1086, 1516
Hpy188I TCNGA 7 cut(s) 192, 621, 931, 1017, 1231, 1278, 1548
Hpy188III TCNNGA 5 cut(s) 497, 602, 648, 940, 1682
Hpy8I GTNNAC 5 cut(s) 49, 284, 1074, 1086, 1516
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 148, 1216, 1523, 1690
HpyCH4IV ACGT 4 cut(s) 86, 147, 1377, 1575
HpyF10VI GCNNNNNNNGC 7 cut(s) 323, 588, 605, 761, 1151, 1216, 1468
HpyF3I CTNAG 6 cut(s) 572, 922, 1628, 1656, 1707, 1730
HpySE526I ACGT 4 cut(s) 86, 147, 1377, 1575
Hsp92II CATG 9 cut(s) 47, 270, 384, 711, 1051, 1466, 1678, 1695, 1821
HspAI GCGC 2 cut(s) 39, 543
Ksp22I TGATCA 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 1074
Kzo9I GATC 5 cut(s) 364, 466, 481, 1582, 1659
LguI GCTCTTC 1 cut(s) 1209
LmnI GCTCC 3 cut(s) 270, 854, 1406
Lsp1109I GCAGC 3 cut(s) 608, 1330, 1713
LweI GCATC 5 cut(s) 367, 530, 1093, 1111, 1505
MaeI CTAG 3 cut(s) 540, 602, 1763
MaeII ACGT 4 cut(s) 86, 147, 1377, 1575
MaeIII GTNAC 4 cut(s) 10, 394, 1571, 1615
MalI GATC 5 cut(s) 366, 468, 483, 1584, 1661
MboI GATC 5 cut(s) 364, 466, 481, 1582, 1659
MboII GAAGA 7 cut(s) 433, 674, 969, 1226, 1346, 1349, 1730
MfeI CAATTG 2 cut(s) 981, 1328
MhlI GDGCHC 2 cut(s) 336, 1627
MlsI TGGCCA 1 cut(s) 1607
MluCI AATT 9 cut(s) 179, 235, 897, 981, 998, 1026, 1328, 1370, 1782
MluNI TGGCCA 1 cut(s) 1607
MlyI GAGTC 2 cut(s) 115, 1002
Mox20I TGGCCA 1 cut(s) 1607
Mph1103I ATGCAT 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 1607
MseI TTAA 7 cut(s) 408, 843, 900, 1073, 1374, 1524, 1665
Msp20I TGGCCA 1 cut(s) 1607
MspI CCGG 3 cut(s) 1404, 1646, 1758
MspR9I CCNGG 3 cut(s) 593, 1058, 1758
MunI CAATTG 2 cut(s) 981, 1328
Mva1269I GAATGC 2 cut(s) 771, 803
MvaI CCWGG 2 cut(s) 593, 1058
MvnI CGCG 2 cut(s) 358, 809
MwoI GCNNNNNNNGC 7 cut(s) 323, 588, 605, 761, 1151, 1216, 1468
NciI CCSGG 1 cut(s) 1758
NcoI CCATGG 1 cut(s) 266
NdeI CATATG 2 cut(s) 655, 1392
NdeII GATC 5 cut(s) 364, 466, 481, 1582, 1659
NlaIII CATG 9 cut(s) 47, 270, 384, 711, 1051, 1466, 1678, 1695, 1821
NlaIV GGNNCC 5 cut(s) 117, 335, 609, 1067, 1701
NmuCI GTSAC 2 cut(s) 10, 1571
NsiI ATGCAT 1 cut(s) 382
NspI RCATGY 2 cut(s) 1466, 1678
NspV TTCGAA 1 cut(s) 197
PaeI GCATGC 1 cut(s) 1466
PaeR7I CTCGAG 1 cut(s) 1356
PciSI GCTCTTC 1 cut(s) 1209
PcsI WCGNNNNNNNCGW 1 cut(s) 1572
PctI GAATGC 2 cut(s) 771, 803
PdmI GAANNNNTTC 1 cut(s) 1248
PfeI GAWTC 7 cut(s) 576, 622, 918, 926, 1249, 1423, 1533
PflMI CCANNNNNTGG 1 cut(s) 687
PfoI TCCNGGA 1 cut(s) 1056
PkrI GCNGC 4 cut(s) 325, 598, 1320, 1728
PleI GAGTC 2 cut(s) 115, 1001
PpsI GAGTC 2 cut(s) 115, 1001
PpuMI RGGWCCY 1 cut(s) 1700
Psp124BI GAGCTC 1 cut(s) 1627
Psp1406I AACGTT 1 cut(s) 1377
Psp5II RGGWCCY 1 cut(s) 1700
Psp6I CCWGG 2 cut(s) 591, 1056
PspFI CCCAGC 1 cut(s) 1427
PspGI CCWGG 2 cut(s) 591, 1056
PspN4I GGNNCC 5 cut(s) 117, 335, 609, 1067, 1701
PspPI GGNCC 6 cut(s) 115, 263, 536, 650, 1387, 1700
PspPPI RGGWCCY 1 cut(s) 1700
PspXI VCTCGAGB 1 cut(s) 1356
RsaI GTAC 3 cut(s) 371, 956, 1596
RsaNI GTAC 3 cut(s) 370, 955, 1595
SacI GAGCTC 1 cut(s) 1627
SapI GCTCTTC 1 cut(s) 1209
SaqAI TTAA 7 cut(s) 408, 843, 900, 1073, 1374, 1524, 1665
SatI GCNGC 4 cut(s) 324, 597, 1319, 1727
Sau3AI GATC 5 cut(s) 364, 466, 481, 1582, 1659
Sau96I GGNCC 6 cut(s) 115, 263, 536, 650, 1387, 1700
SchI GAGTC 2 cut(s) 115, 1002
ScrFI CCNGG 3 cut(s) 593, 1058, 1758
SduI GDGCHC 2 cut(s) 336, 1627
SfaNI GCATC 5 cut(s) 367, 530, 1093, 1111, 1505
Sfr274I CTCGAG 1 cut(s) 1356
SfuI TTCGAA 1 cut(s) 197
SinI GGWCC 3 cut(s) 263, 650, 1700
SlaI CTCGAG 1 cut(s) 1356
SmlI CTYRAG 2 cut(s) 1356, 1680
SmoI CTYRAG 2 cut(s) 1356, 1680
SphI GCATGC 1 cut(s) 1466
Sse9I AATT 9 cut(s) 179, 235, 897, 981, 998, 1026, 1328, 1370, 1782
SsiI CCGC 4 cut(s) 324, 356, 809, 1506
SspI AATATT 1 cut(s) 641
SspMI CTAG 3 cut(s) 540, 602, 1763
SstI GAGCTC 1 cut(s) 1627
StyD4I CCNGG 3 cut(s) 591, 1056, 1756
StyI CCWWGG 3 cut(s) 266, 790, 1797
TaiI ACGT 4 cut(s) 89, 150, 1380, 1578
TaqI TCGA 7 cut(s) 164, 197, 498, 524, 996, 1357, 1566
TasI AATT 9 cut(s) 179, 235, 897, 981, 998, 1026, 1328, 1370, 1782
TatI WGTACW 2 cut(s) 369, 1594
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 7 cut(s) 576, 622, 918, 926, 1249, 1423, 1533
Tru1I TTAA 7 cut(s) 408, 843, 900, 1073, 1374, 1524, 1665
Tru9I TTAA 7 cut(s) 408, 843, 900, 1073, 1374, 1524, 1665
TscAI CASTG 6 cut(s) 19, 452, 888, 1119, 1176, 1525
TseFI GTSAC 2 cut(s) 10, 1571
TseI GCWGC 3 cut(s) 596, 1318, 1726
Tsp45I GTSAC 2 cut(s) 10, 1571
TspDTI ATGAA 7 cut(s) 80, 248, 664, 850, 923, 1189, 1249
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 6 cut(s) 19, 452, 888, 1119, 1176, 1525
Van91I CCANNNNNTGG 1 cut(s) 687
VpaK11BI GGWCC 3 cut(s) 263, 650, 1700
XagI CCTNNNNNAGG 2 cut(s) 1191, 1796
XapI RAATTY 3 cut(s) 235, 1026, 1370
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 2 cut(s) 1466, 1678
XhoI CTCGAG 1 cut(s) 1356
XmiI GTMKAC 2 cut(s) 1085, 1515
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 3 cut(s) 540, 602, 1763
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.