Rorug01G0321000

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008283
Physical Location & Seq
Forward (+)
44012701 .. 44022730
10030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0321000.1

Sequence Viewer

Length: 5823 bp
ATGACCAAGAAGCTCACACAATCGGAACCACAACTCGCCATGGAGCTCGACCTCGACTCGTTCCTCAACTCACACCTCTCCTCCTCCTCCTCTGACTCCGACCCCGACCTCACCTCCGTCCCTCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCCCCCTCCTCTTCCCCGCGTAGTTCTACCTTATCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGCGGAAGAACCGAGGGAGCGGCGCGGTTGAGTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCCGGGGATTTCCCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCCGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCGCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAGGTTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGGGGATAGCAATGCGGTTGGTTCCGAAACGATGCCGTCGAATTCGAATGAAGTAGAGGAGGATTTTGGTGGTGAGTTGGGGAGGAAAGATGAGGTTTTGGAGAGGGAGAGGGAGGTAGAGCAGGTGAGTCAGGTTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACAAATTTAGATGCAACTGATGTTAAAGATAATGAATTGGATGAGAATGTTCGGGTTTCGGAGGAAGGTCCACCAGAGGAAGAAGATTTAGATGAAAATAGTCTTTCTTTGAAACATGGTGATGTTAAGGATGAGCCTACTGGTGAGGATGAGCAGCATCTTGGTGGTAATAATGGTGATGATGAGGTTGGTGATAATGACAATGATAAAGATGGTGAGGATCATTTTGATGATGATGATGATGCATTGGGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTAGAGAGCAGGAGGATTAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCCGAAGAGCTAGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAACCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATATTTCAATGTTGATGCTAAAAATACCATTACGCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCACAAGTACTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCGAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTAACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCCGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCCCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACTCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAAGCTCTTCACTTTCCTCTCAGGGAAATGCGATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTATTTGTCACCCATCAAAATGCTTTGGTGGTAAGACTAACTCCCACTTTGCATGTCTATGCACAACTTTCTAAACCAGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTGCTTGGAAATGCACAACTCAATTGCATAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGTAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGCTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGCCTTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCGCCTGAGATTATGCAAGCTCTGGTAGAACATTACAGTCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCACAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTATTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAGCTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAACCTCGAAATGGGACTTCTCTTCTCAAAATTCAGCTGATGCAGATACAGAGCTGCAGTATGGGAATAATTCAACGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTCGTAGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCAGAGAATAACTTTCCAACGTCTGTTTCTGGAGATAGCATGATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCGATCAGCAGTTATTTCTAGAATTCCAGAGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAGTGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCCCATCCACGAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCTGGCACCCTCGACTTCTCAAGTTTAAAAACCGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTGGAAAGGGTCTCTAGTTTCCCACAGCTTTTGCGTGATAGTCCTATTAATTTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGCGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTGTTGGTAGTCTAAATTTAAGTGGGGCTTCACGTGGTTCTGCTAGTACAGAATACTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACGGTATCTAAAGGAGAAGATCATAATAGAATGGTAACCAATTCATTGCATTCACAAGAGGATGAAATTAATTTCACCATCAAGTGGAGAATCCCAAAGGCTCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGGTATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTCGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTATACTATGAGCATCCTCAAGAAAGGAGCCTCACATGGGTATGCTCCTCGCAACCCAAAATGTTGCATATGCGATTGCCTCCTTGACAAGAACTCTTCCAGCTACATTCGCATTTTCAATTGTGGTCATGCAACACATCTTAAATGTGAAGTTTCAGAGAATGAAACATCAAGTCGTAGCTCGTCCTCTGGATGCCCAGTTTGTATGCCCAAGAAGAAATCTCAGAGGTCTAAAAACAAATCGGTTCTAGCAGAGGAGAGTTTAGTGAAGGTGTTTTCATCAAGAACCCATCATACTCATGGAACAGTTGTTCACCCACATGAAAGCACTTCTGAGAATAGCTATGGGATTCAGCAGATATCACGATTTGAGATGTTGACCAATTTGCAGAAACATCGTGGATTAGTCGAGATTGAAAACATGCCTCAATTGAGGCTTGCCCCACCAGCTGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGCAGTGATCTTGTGAGAACAGAGAAACAAAGTAAAATCAAGCAACTCAGGGAGGTAAAAGTAAAAGGATCATCTCTTCGGTTTCCCTTGAAAACAAACTTATTTGGTAATGGCAAGGACAAGATCAGCAGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

1940

Amino Acids

214.25

Weight (kDa)

5.36

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 422 - 606 9.7e-23 Vps8 beta-propeller
VPS8_N PF23306 423 - 596 1.7e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 450 - 542 6.9e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 970 - 1153 7.8e-51 Vps8 central TPR
TPR_Vps41 PF23556 1465 - 1533 1.2e-06 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1585 - 1732 2.5e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1407, 2430
AarI CACCTGC 1 cut(s) 709
AasI GACNNNNNNGTC 1 cut(s) 374
Acc36I ACCTGC 3 cut(s) 709, 1892, 3835
AccB1I GGYRCC 1 cut(s) 4256
AccB7I CCANNNNNTGG 1 cut(s) 4935
AccBSI CCGCTC 3 cut(s) 284, 528, 1459
AccII CGCG 9 cut(s) 193, 221, 289, 446, 452, 497, 1276, 3087, 4479
AcoI YGGCCR 2 cut(s) 320, 2587
AcuI CTGAAG 2 cut(s) 2145, 3447
AcvI CACGTG 1 cut(s) 4655
AcyI GRCGYC 2 cut(s) 249, 485
AflIII ACRYGT 1 cut(s) 1643
AjnI CCWGG 9 cut(s) 164, 2655, 3108, 3265, 3379, 3930, 3978, 5076, 5674
AjuI GAANNNNNNNTTGG 4 cut(s) 3790, 3822, 4608, 4640
AloI GAACNNNNNNTCC 2 cut(s) 5478, 5510
Alw21I GWGCWC 5 cut(s) 48, 2778, 3364, 4488, 4570
Alw26I GTCTC 7 cut(s) 1198, 2229, 2895, 3745, 3869, 4348, 4454
Alw44I GTGCAC 2 cut(s) 2774, 4566
AlwNI CAGNNNCTG 2 cut(s) 1664, 2272
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 9 cut(s) 320, 392, 441, 2587, 3204, 3250, 3668, 3853, 3940
ApaLI GTGCAC 2 cut(s) 2774, 4566
ArsI GACNNNNNNTTYG 2 cut(s) 2843, 2875
AseI ATTAAT 2 cut(s) 4380, 4920
Asp700I GAANNNNTTC 5 cut(s) 1819, 1913, 2520, 3140, 4011
AspLEI GCGC 3 cut(s) 289, 448, 521
AsuC2I CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2497
AsuII TTCGAA 2 cut(s) 641, 5133
AsuNHI GCTAGC 1 cut(s) 3635
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 3 cut(s) 2778, 3109, 4570
BalI TGGCCA 1 cut(s) 2589
BamHI GGATCC 1 cut(s) 1925
BanI GGYRCC 1 cut(s) 4256
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 4655
BbsI GAAGAC 1 cut(s) 3752
Bbv12I GWGCWC 5 cut(s) 48, 2778, 3364, 4488, 4570
BceAI ACGGC 6 cut(s) 111, 236, 379, 385, 616, 3561
BcgI CGANNNNNNTGC 8 cut(s) 460, 494, 3689, 3723, 4049, 4083, 5561, 5595
BciT130I CCWGG 9 cut(s) 166, 2657, 3110, 3267, 3381, 3932, 3980, 5078, 5676
BciVI GTATCC 3 cut(s) 1514, 3206, 5143
BclI TGATCA 3 cut(s) 1306, 2263, 3688
BcnI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2497
BcoDI GTCTC 7 cut(s) 1198, 2229, 2895, 3745, 3869, 4348, 4454
BfmI CTRYAG 1 cut(s) 3524
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 3 cut(s) 709, 1892, 3835
BfuI GTATCC 3 cut(s) 1514, 3206, 5143
BglII AGATCT 1 cut(s) 2868
BlpI GCTNAGC 1 cut(s) 5117
BmcAI AGTACT 3 cut(s) 1326, 2770, 3239
BmeT110I CYCGRG 1 cut(s) 437
BmrI ACTGGG 4 cut(s) 1337, 1383, 1674, 5375
BmtI GCTAGC 1 cut(s) 3639
BmuI ACTGGG 4 cut(s) 1337, 1383, 1674, 5375
BpiI GAAGAC 1 cut(s) 3752
BplI GAGNNNNNCTC 2 cut(s) 3378, 3410
BpmI CTGGAG 3 cut(s) 1584, 3831, 4433
Bpu1102I GCTNAGC 1 cut(s) 5117
Bpu14I TTCGAA 2 cut(s) 641, 5133
BpuEI CTTGAG 6 cut(s) 1229, 3352, 3881, 4255, 4699, 5186
BpuMI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2497
BsaAI YACGTR 2 cut(s) 1646, 4655
BsaBI GATNNNNATC 3 cut(s) 3063, 3687, 4473
BsaHI GRCGYC 2 cut(s) 249, 485
BsaI GGTCTC 2 cut(s) 2229, 4348
BsaWI WCCGGW 1 cut(s) 1583
Bse3DI GCAATG 7 cut(s) 613, 757, 1347, 2577, 3032, 4690, 4895
Bse8I GATNNNNATC 3 cut(s) 3063, 3687, 4473
BseBI CCWGG 9 cut(s) 166, 2657, 3110, 3267, 3381, 3932, 3980, 5078, 5676
BseJI GATNNNNATC 3 cut(s) 3063, 3687, 4473
BseMI GCAATG 7 cut(s) 613, 757, 1347, 2577, 3032, 4690, 4895
BseSI GKGCMC 3 cut(s) 2778, 3109, 4570
BseX3I CGGCCG 1 cut(s) 320
BsgI GTGCAG 2 cut(s) 2256, 4185
Bsh1236I CGCG 9 cut(s) 193, 221, 289, 446, 452, 497, 1276, 3087, 4479
Bsh1285I CGRYCG 4 cut(s) 135, 261, 323, 378
BshFI GGCC 9 cut(s) 322, 394, 443, 2589, 3206, 3252, 3670, 3855, 3942
BshNI GGYRCC 1 cut(s) 4256
BsiEI CGRYCG 4 cut(s) 135, 261, 323, 378
BsiHKAI GWGCWC 5 cut(s) 48, 2778, 3364, 4488, 4570
BsiHKCI CYCGRG 1 cut(s) 437
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 4 cut(s) 104, 1370, 3497, 5146
BsmAI GTCTC 7 cut(s) 1198, 2229, 2895, 3745, 3869, 4348, 4454
BsmFI GGGAC 4 cut(s) 104, 1370, 3497, 5146
BsmI GAATGC 5 cut(s) 433, 2711, 4133, 4439, 4900
BsnI GGCC 9 cut(s) 322, 394, 443, 2589, 3206, 3252, 3670, 3855, 3942
Bso31I GGTCTC 2 cut(s) 2229, 4348
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 2 cut(s) 641, 5133
Bsp1286I GDGCHC 6 cut(s) 48, 2778, 3109, 3364, 4488, 4570
Bsp1407I TGTACA 3 cut(s) 336, 1606, 2632
Bsp1720I GCTNAGC 1 cut(s) 5117
Bsp19I CCATGG 2 cut(s) 39, 2507
BspANI GGCC 9 cut(s) 322, 394, 443, 2589, 3206, 3252, 3670, 3855, 3942
BspFNI CGCG 9 cut(s) 193, 221, 289, 446, 452, 497, 1276, 3087, 4479
BspMAI CTGCAG 1 cut(s) 3528
BspMI ACCTGC 3 cut(s) 709, 1892, 3835
BspOI GCTAGC 1 cut(s) 3639
BspQI GCTCTTC 2 cut(s) 1131, 1825
BspT104I TTCGAA 2 cut(s) 641, 5133
BspT107I GGYRCC 1 cut(s) 4256
BspTNI GGTCTC 2 cut(s) 2229, 4348
BsrBI CCGCTC 3 cut(s) 284, 528, 1459
BsrDI GCAATG 7 cut(s) 613, 757, 1347, 2577, 3032, 4690, 4895
BsrGI TGTACA 3 cut(s) 336, 1606, 2632
BssNI GRCGYC 2 cut(s) 249, 485
BssT1I CCWWGG 3 cut(s) 39, 2507, 3208
Bst2UI CCWGG 9 cut(s) 166, 2657, 3110, 3267, 3381, 3932, 3980, 5078, 5676
BstACI GRCGYC 2 cut(s) 249, 485
BstAPI GCANNNNNTGC 1 cut(s) 1568
BstAUI TGTACA 3 cut(s) 336, 1606, 2632
BstBAI YACGTR 2 cut(s) 1646, 4655
BstBI TTCGAA 2 cut(s) 641, 5133
BstDSI CCRYGG 4 cut(s) 39, 401, 495, 2507
BstEII GGTNACC 2 cut(s) 3147, 4885
BstENI CCTNNNNNAGG 1 cut(s) 3860
BstFNI CGCG 9 cut(s) 193, 221, 289, 446, 452, 497, 1276, 3087, 4479
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 3 cut(s) 289, 448, 521
BstMAI GTCTC 7 cut(s) 1198, 2229, 2895, 3745, 3869, 4348, 4454
BstMCI CGRYCG 4 cut(s) 135, 261, 323, 378
BstNI CCWGG 9 cut(s) 166, 2657, 3110, 3267, 3381, 3932, 3980, 5078, 5676
BstNSI RCATGY 6 cut(s) 2009, 2947, 3726, 4733, 4737, 5608
BstPI GGTNACC 2 cut(s) 3147, 4885
BstSFI CTRYAG 1 cut(s) 3524
BstSLI GKGCMC 3 cut(s) 2778, 3109, 4570
BstUI CGCG 9 cut(s) 193, 221, 289, 446, 452, 497, 1276, 3087, 4479
BstV2I GAAGAC 1 cut(s) 3752
BstX2I RGATCY 4 cut(s) 1504, 1925, 2868, 3629
BstXI CCANNNNNNTGG 6 cut(s) 1237, 2248, 2597, 3626, 3938, 5084
BstYI RGATCY 4 cut(s) 1504, 1925, 2868, 3629
BstZI CGGCCG 1 cut(s) 320
BsuI GTATCC 3 cut(s) 1514, 3206, 5143
BsuRI GGCC 9 cut(s) 322, 394, 443, 2589, 3206, 3252, 3670, 3855, 3942
BtgI CCRYGG 4 cut(s) 39, 401, 495, 2507
BtgZI GCGATG 1 cut(s) 1859
BtsI GCAGTG 2 cut(s) 3646, 5695
BtsIMutI CAGTG 7 cut(s) 1748, 2778, 3646, 4168, 4239, 4486, 5695
BveI ACCTGC 3 cut(s) 709, 1892, 3835
CaiI CAGNNNCTG 2 cut(s) 1664, 2272
CfoI GCGC 3 cut(s) 289, 448, 521
Cfr42I CCGCGG 1 cut(s) 498
Cfr9I CCCGGG 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 3 cut(s) 210, 257, 493
CsiI ACCWGGT 2 cut(s) 2655, 3930
CspCI CAANNNNNGTGG 2 cut(s) 4344, 4379
CspI CGGWCCG 1 cut(s) 316
DraI TTTAAA 2 cut(s) 4278, 5095
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 320, 2587
EagI CGGCCG 1 cut(s) 320
EciI GGCGGA 1 cut(s) 2949
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 320
Eco130I CCWWGG 3 cut(s) 39, 2507, 3208
Eco147I AGGCCT 1 cut(s) 3252
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 2229, 4348
Eco32I GATATC 1 cut(s) 5544
Eco52I CGGCCG 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 2145, 3447
Eco72I CACGTG 1 cut(s) 4655
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 2 cut(s) 3147, 4885
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3860
EcoO65I GGTNACC 2 cut(s) 3147, 4885
EcoRI GAATTC 7 cut(s) 637, 775, 2825, 3374, 3886, 4098, 5142
EcoRII CCWGG 9 cut(s) 164, 2655, 3108, 3265, 3379, 3930, 3978, 5076, 5674
EcoRV GATATC 1 cut(s) 5544
EcoT14I CCWWGG 3 cut(s) 39, 2507, 3208
EcoT22I ATGCAT 2 cut(s) 1027, 4735
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 39, 2507, 3208
FalI AAGNNNNNCTT 6 cut(s) 2440, 2472, 3227, 3259, 4633, 4665
FaqI GGGAC 4 cut(s) 104, 1370, 3497, 5146
FauI CCCGC 2 cut(s) 198, 521
FauNDI CATATG 3 cut(s) 2677, 5125, 5252
FbaI TGATCA 3 cut(s) 1306, 2263, 3688
FriOI GRGCYC 1 cut(s) 48
GlaI GCGC 3 cut(s) 288, 447, 520
GsuI CTGGAG 3 cut(s) 1584, 3831, 4433
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 9 cut(s) 322, 394, 443, 2589, 3206, 3252, 3670, 3855, 3942
HgaI GACGC 3 cut(s) 210, 257, 493
HhaI GCGC 3 cut(s) 289, 448, 521
Hin1I GRCGYC 2 cut(s) 249, 485
Hin6I GCGC 3 cut(s) 287, 446, 519
HinP1I GCGC 3 cut(s) 287, 446, 519
HincII GTYRAC 4 cut(s) 2608, 3646, 4053, 5562
HindII GTYRAC 4 cut(s) 2608, 3646, 4053, 5562
HindIII AAGCTT 3 cut(s) 1155, 2424, 4610
Hpy99I CGWCG 5 cut(s) 140, 251, 400, 487, 637
HpyCH4IV ACGT 3 cut(s) 1645, 3800, 4654
HpySE526I ACGT 3 cut(s) 1645, 3800, 4654
Hsp92I GRCGYC 2 cut(s) 249, 485
HspAI GCGC 3 cut(s) 287, 446, 519
Ksp22I TGATCA 3 cut(s) 1306, 2263, 3688
KspI CCGCGG 1 cut(s) 498
LguI GCTCTTC 2 cut(s) 1131, 1825
MabI ACCWGGT 2 cut(s) 2655, 3930
MaeII ACGT 3 cut(s) 1645, 3800, 4654
MbiI CCGCTC 3 cut(s) 284, 528, 1459
MfeI CAATTG 7 cut(s) 299, 2084, 2154, 2434, 3579, 5302, 5612
MflI RGATCY 4 cut(s) 1504, 1925, 2868, 3629
MhlI GDGCHC 6 cut(s) 48, 2778, 3109, 3364, 4488, 4570
MlsI TGGCCA 1 cut(s) 2589
MluNI TGGCCA 1 cut(s) 2589
MlyI GAGTC 7 cut(s) 50, 89, 304, 733, 1768, 3641, 4805
MmeI TCCRAC 7 cut(s) 123, 1886, 2238, 3821, 4521, 4540, 5063
Mox20I TGGCCA 1 cut(s) 2589
Mph1103I ATGCAT 2 cut(s) 1027, 4735
MroXI GAANNNNTTC 5 cut(s) 1819, 1913, 2520, 3140, 4011
MscI TGGCCA 1 cut(s) 2589
Msp20I TGGCCA 1 cut(s) 2589
MspA1I CMGCKG 5 cut(s) 267, 497, 1568, 3506, 5633
MunI CAATTG 7 cut(s) 299, 2084, 2154, 2434, 3579, 5302, 5612
Mva1269I GAATGC 5 cut(s) 433, 2711, 4133, 4439, 4900
MvaI CCWGG 9 cut(s) 166, 2657, 3110, 3267, 3381, 3932, 3980, 5078, 5676
MvnI CGCG 9 cut(s) 193, 221, 289, 446, 452, 497, 1276, 3087, 4479
NciI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2497
NcoI CCATGG 2 cut(s) 39, 2507
NdeI CATATG 3 cut(s) 2677, 5125, 5252
NheI GCTAGC 1 cut(s) 3635
NmuCI GTSAC 8 cut(s) 1532, 1639, 1960, 2543, 2996, 3147, 3185, 5670
NsiI ATGCAT 2 cut(s) 1027, 4735
NspI RCATGY 6 cut(s) 2009, 2947, 3726, 4733, 4737, 5608
NspV TTCGAA 2 cut(s) 641, 5133
PaeI GCATGC 1 cut(s) 4733
PaqCI CACCTGC 1 cut(s) 709
PceI AGGCCT 1 cut(s) 3252
PciSI GCTCTTC 2 cut(s) 1131, 1825
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 5 cut(s) 433, 2711, 4133, 4439, 4900
PdmI GAANNNNTTC 5 cut(s) 1819, 1913, 2520, 3140, 4011
Pfl23II CGTACG 1 cut(s) 128
PflFI GACNNNGTC 1 cut(s) 1777
PflMI CCANNNNNTGG 1 cut(s) 4935
PfoI TCCNGGA 1 cut(s) 363
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 7 cut(s) 50, 89, 303, 732, 1768, 3641, 4805
PmaCI CACGTG 1 cut(s) 4655
PmlI CACGTG 1 cut(s) 4655
PpsI GAGTC 7 cut(s) 50, 89, 303, 732, 1768, 3641, 4805
Ppu21I YACGTR 2 cut(s) 1646, 4655
PshBI ATTAAT 2 cut(s) 4380, 4920
PsiI TTATAA 2 cut(s) 1407, 2430
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 9 cut(s) 164, 2655, 3108, 3265, 3379, 3930, 3978, 5076, 5674
PspCI CACGTG 1 cut(s) 4655
PspEI GGTNACC 2 cut(s) 3147, 4885
PspGI CCWGG 9 cut(s) 164, 2655, 3108, 3265, 3379, 3930, 3978, 5076, 5674
PspLI CGTACG 1 cut(s) 128
PsrI GAACNNNNNNTAC 4 cut(s) 1317, 1349, 5461, 5493
PstI CTGCAG 1 cut(s) 3528
PstNI CAGNNNCTG 2 cut(s) 1664, 2272
PsuI RGATCY 4 cut(s) 1504, 1925, 2868, 3629
PsyI GACNNNGTC 1 cut(s) 1777
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 2 cut(s) 3506, 5633
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 498
SapI GCTCTTC 2 cut(s) 1131, 1825
ScaI AGTACT 3 cut(s) 1326, 2770, 3239
SchI GAGTC 7 cut(s) 50, 89, 304, 733, 1768, 3641, 4805
SduI GDGCHC 6 cut(s) 48, 2778, 3109, 3364, 4488, 4570
SexAI ACCWGGT 2 cut(s) 2655, 3930
SfcI CTRYAG 1 cut(s) 3524
Sfr303I CCGCGG 1 cut(s) 498
SfuI TTCGAA 2 cut(s) 641, 5133
SgrBI CCGCGG 1 cut(s) 498
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 6 cut(s) 1208, 3367, 3860, 4270, 4678, 5201
SmoI CTYRAG 6 cut(s) 1208, 3367, 3860, 4270, 4678, 5201
SphI GCATGC 1 cut(s) 4733
SseBI AGGCCT 1 cut(s) 3252
SspI AATATT 1 cut(s) 5106
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3252
StyI CCWWGG 3 cut(s) 39, 2507, 3208
TaiI ACGT 3 cut(s) 1648, 3803, 4657
TaqII GACCGA 2 cut(s) 247, 364
TscAI CASTG 7 cut(s) 1748, 2785, 3646, 4168, 4239, 4493, 5695
TseFI GTSAC 8 cut(s) 1532, 1639, 1960, 2543, 2996, 3147, 3185, 5670
Tsp45I GTSAC 8 cut(s) 1532, 1639, 1960, 2543, 2996, 3147, 3185, 5670
TspGWI ACGGA 5 cut(s) 106, 2533, 2548, 4768, 5673
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 7 cut(s) 1748, 2785, 3646, 4168, 4239, 4493, 5695
Tth111I GACNNNGTC 1 cut(s) 1777
Van91I CCANNNNNTGG 1 cut(s) 4935
VneI GTGCAC 2 cut(s) 2774, 4566
VspI ATTAAT 2 cut(s) 4380, 4920
XagI CCTNNNNNAGG 1 cut(s) 3860
XbaI TCTAGA 3 cut(s) 1750, 2350, 4094
XceI RCATGY 6 cut(s) 2009, 2947, 3726, 4733, 4737, 5608
XcmI CCANNNNNNNNNTGG 1 cut(s) 5480
XmaI CCCGGG 1 cut(s) 437
XmnI GAANNNNTTC 5 cut(s) 1819, 1913, 2520, 3140, 4011
ZrmI AGTACT 3 cut(s) 1326, 2770, 3239
Zsp2I ATGCAT 2 cut(s) 1027, 4735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.