Rorug01G0320800

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008283
Physical Location & Seq
Forward (+)
44000515 .. 44001726
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0320800.1

Sequence Viewer

Length: 333 bp
ATGTCGATTTCAGAGATTGCTTGCACCTACGCCGCTTTGATCCTCCACGACGATGGCATCCCCATCACTGCTGAGAAGATTGCCGCTTTGGTCAAAGCTGCCAATGTCTCTGTCGAGTCCTACTGGCCTGGTTTGTTTGCCAAGCTTGCCGAGAAGAGAGACATCGGTGACCTCATCTTGAATGTTGGTGCCGGAGGCGGTGGTGGTGCTGTTGCCGTTGCTGCCCCAGGTGCAGGCGCCGCCGCCCCTGCTGCAGCTGCTCCAGTGGAAGAGAAGAAGGAGGAGCCAAAGGAAGAGAGTGATGAAGATATGGGATTCAGCTTGTTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

110

Amino Acids

11.11

Weight (kDa)

4.36

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_60s PF00428 22 - 109 2.1e-27 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 236
AciI CCGC 5 cut(s) 33, 84, 198, 240, 243
AclWI GGATC 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 233
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 2 cut(s) 127, 226
AluBI AGCT 4 cut(s) 98, 145, 257, 321
AluI AGCT 4 cut(s) 98, 145, 257, 321
Alw26I GTCTC 2 cut(s) 112, 153
AlwI GGATC 1 cut(s) 34
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 5 cut(s) 98, 221, 251, 254, 257
AspLEI GCGC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 179
BanI GGYRCC 2 cut(s) 188, 236
BbvI GCAGC 5 cut(s) 85, 208, 238, 244, 266
BccI CCATC 2 cut(s) 47, 71
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 2 cut(s) 129, 228
BcoDI GTCTC 2 cut(s) 112, 153
BfaI CTAG 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 240
BisI GCNGC 9 cut(s) 33, 84, 99, 222, 240, 243, 252, 255, 258
BlsI GCNGC 9 cut(s) 34, 85, 100, 223, 241, 244, 253, 256, 259
Bme1390I CCNGG 2 cut(s) 129, 228
BmiI GGNNCC 3 cut(s) 190, 238, 285
BmrFI CCNGG 2 cut(s) 129, 228
BmsI GCATC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 128, 263
BseBI CCWGG 2 cut(s) 129, 228
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 2 cut(s) 128, 263
BseRI GAGGAG 1 cut(s) 296
BseXI GCAGC 5 cut(s) 85, 208, 238, 244, 266
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 1 cut(s) 127
BshNI GGYRCC 2 cut(s) 188, 236
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 2 cut(s) 112, 153
BsnI GGCC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 39
BspACI CCGC 5 cut(s) 33, 84, 198, 240, 243
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 3 cut(s) 190, 238, 285
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 1 cut(s) 34
BspT107I GGYRCC 2 cut(s) 188, 236
BsrI ACTGG 2 cut(s) 128, 263
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 39
BssNI GRCGYC 1 cut(s) 237
Bst2UI CCWGG 2 cut(s) 129, 228
Bst6I CTCTTC 3 cut(s) 149, 264, 288
BstACI GRCGYC 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 22, 147, 235
BstDEI CTNAG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 2 cut(s) 112, 153
BstMBI GATC 1 cut(s) 39
BstMWI GCNNNNNNNGC 7 cut(s) 146, 221, 230, 239, 248, 251, 257
BstNI CCWGG 2 cut(s) 129, 228
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 2 cut(s) 127, 226
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 5 cut(s) 85, 208, 238, 244, 266
BstXI CCANNNNNNTGG 1 cut(s) 53
BsuRI GGCC 1 cut(s) 127
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 270
Cac8I GCNNGC 3 cut(s) 22, 147, 235
CfoI GCGC 1 cut(s) 239
CviJI RGCY 6 cut(s) 98, 127, 145, 257, 286, 321
CviKI_1 RGCY 6 cut(s) 98, 127, 145, 257, 286, 321
DdeI CTNAG 1 cut(s) 72
DinI GGCGCC 1 cut(s) 238
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 149, 264, 288
EarI CTCTTC 3 cut(s) 149, 264, 288
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 127, 226
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
FaiI YATR 1 cut(s) 311
Fnu4HI GCNGC 9 cut(s) 33, 84, 99, 222, 240, 243, 252, 255, 258
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 9 cut(s) 33, 84, 99, 222, 240, 243, 252, 255, 258
FspBI CTAG 1 cut(s) 331
GlaI GCGC 1 cut(s) 238
GluI GCNGC 9 cut(s) 33, 84, 99, 222, 240, 243, 252, 255, 258
GsuI CTGGAG 1 cut(s) 246
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 1 cut(s) 127
HapII CCGG 1 cut(s) 192
HhaI GCGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 237
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 2 cut(s) 116, 315
HpaII CCGG 1 cut(s) 192
HphI GGTGA 1 cut(s) 179
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 1 cut(s) 178
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 1 cut(s) 271
HpyCH4V TGCA 3 cut(s) 24, 233, 254
HpyF10VI GCNNNNNNNGC 7 cut(s) 146, 221, 230, 239, 248, 251, 257
HpyF3I CTNAG 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 237
HspAI GCGC 1 cut(s) 237
KasI GGCGCC 1 cut(s) 236
Kzo9I GATC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 265, 283
LpnPI CCDG 9 cut(s) 109, 114, 141, 205, 213, 219, 240, 261, 276
Lsp1109I GCAGC 5 cut(s) 85, 208, 238, 244, 266
LweI GCATC 1 cut(s) 66
MaeI CTAG 1 cut(s) 331
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 6 cut(s) 88, 166, 281, 286, 305, 317
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 4 cut(s) 53, 182, 188, 274
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 2 cut(s) 129, 228
MvaI CCWGG 2 cut(s) 129, 228
MwoI GCNNNNNNNGC 7 cut(s) 146, 221, 230, 239, 248, 251, 257
NarI GGCGCC 1 cut(s) 237
NdeII GATC 1 cut(s) 39
NlaIV GGNNCC 3 cut(s) 190, 238, 285
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 315
PkrI GCNGC 9 cut(s) 34, 85, 100, 223, 241, 244, 253, 256, 259
PleI GAGTC 1 cut(s) 124
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 2 cut(s) 127, 226
PspEI GGTNACC 1 cut(s) 167
PspGI CCWGG 2 cut(s) 127, 226
PspN4I GGNNCC 3 cut(s) 190, 238, 285
PstI CTGCAG 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 51
SatI GCNGC 9 cut(s) 33, 84, 99, 222, 240, 243, 252, 255, 258
Sau3AI GATC 1 cut(s) 39
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 129, 228
SetI ASST 7 cut(s) 29, 100, 147, 174, 232, 259, 323
SfaNI GCATC 1 cut(s) 66
SfcI CTRYAG 1 cut(s) 252
SfoI GGCGCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 51
SsiI CCGC 5 cut(s) 33, 84, 198, 240, 243
SspDI GGCGCC 1 cut(s) 236
SspMI CTAG 1 cut(s) 331
StyD4I CCNGG 2 cut(s) 127, 226
TaqI TCGA 3 cut(s) 5, 114, 327
TauI GCSGC 4 cut(s) 35, 86, 242, 245
TfiI GAWTC 1 cut(s) 315
TscAI CASTG 2 cut(s) 73, 270
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 5 cut(s) 98, 221, 251, 254, 257
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 318
TspRI CASTG 2 cut(s) 73, 270
XspI CTAG 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.