pycom01g14610

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008283
Physical Location & Seq
Forward (+)
14726042 .. 14727388
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14610.3

Sequence Viewer

Length: 516 bp
ATGGCTTCTCTACCGTACGCCGACGTGGATTTCAGCCTGAGATCAATGGCCGGTCGGGCCGTGGGCTTCGGCCGGTTCGTCATTGGTGGCCTGAACAGGCCGGTCTATTTTGTGACCACATTGGCAGATGATGGTCCAAGCTCACTTCGTGAGGGGTGCCGAAAACAAGAACCGCTCTGGATTGTGTTTGAGGTTTCTGGCACCATTCACCTTTCATCTTACTTGAGTGTGTCGTCTTACAAGACATTTGATGGACGAGGCCAGAGGATAAAGCTCACGGGCAAGGGCCTTAGGCTGAAGGAATGTGAGCATATAATTGTATGCAACCTCGAGTTTGAAGGTGGTAGAGGACATGATGTCGATGGCATTCAGATCAAACCGAACTCTAGGCACATATGGATAGACCGGTGCAGCCTTCGTGATTGCGACGATGGACTCATAGATATCACCAGACAAAGTACAGAGATAACTGTCTCTAGGTATGTTACTTTGCGCAACATGACAAGACGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

172

Amino Acids

19.25

Weight (kDa)

8.81

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 494
AccB1I GGYRCC 2 cut(s) 156, 200
AccBSI CCGCTC 1 cut(s) 175
AciI CCGC 1 cut(s) 173
AcoI YGGCCR 2 cut(s) 48, 70
AcuI CTGAAG 1 cut(s) 317
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 2 cut(s) 17, 460
AgeI ACCGGT 1 cut(s) 405
AgsI TTSAA 1 cut(s) 338
AhdI GACNNNNNGTC 1 cut(s) 356
AjiI CACGTC 1 cut(s) 25
AluBI AGCT 2 cut(s) 141, 274
AluI AGCT 2 cut(s) 141, 274
Alw26I GTCTC 1 cut(s) 478
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 7 cut(s) 48, 57, 70, 88, 98, 259, 286
ApeKI GCWGC 1 cut(s) 411
AsiGI ACCGGT 1 cut(s) 405
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 3 cut(s) 57, 134, 286
AsuHPI GGTGA 2 cut(s) 200, 439
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 134
AxyI CCTNAGG 1 cut(s) 290
BanI GGYRCC 2 cut(s) 156, 200
BbvI GCAGC 1 cut(s) 423
BccI CCATC 4 cut(s) 125, 245, 356, 425
BceAI ACGGC 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 478
BfaI CTAG 2 cut(s) 387, 477
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 1 cut(s) 412
BlsI GCNGC 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 134
BmeRI GACNNNNNGTC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 57, 134, 286
BmiI GGNNCC 2 cut(s) 158, 202
BpuEI CTTGAG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 405
Bse118I RCCGGY 4 cut(s) 50, 72, 100, 405
Bse21I CCTNAGG 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 29
BseX3I CGGCCG 1 cut(s) 70
BseXI GCAGC 1 cut(s) 423
BsgI GTGCAG 1 cut(s) 430
Bsh1285I CGRYCG 2 cut(s) 55, 73
BshFI GGCC 7 cut(s) 50, 59, 72, 90, 100, 261, 288
BshNI GGYRCC 2 cut(s) 156, 200
BshTI ACCGGT 1 cut(s) 405
BsiEI CGRYCG 2 cut(s) 55, 73
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 4 cut(s) 51, 73, 101, 406
BsiWI CGTACG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 7 cut(s) 50, 59, 72, 90, 100, 261, 288
BsoBI CYCGRG 1 cut(s) 329
Bsp143I GATC 2 cut(s) 41, 372
BspACI CCGC 1 cut(s) 173
BspANI GGCC 7 cut(s) 50, 59, 72, 90, 100, 261, 288
BspCNI CTCAG 1 cut(s) 30
BspLI GGNNCC 2 cut(s) 158, 202
BspT107I GGYRCC 2 cut(s) 156, 200
BsrBI CCGCTC 1 cut(s) 175
BsrFI RCCGGY 4 cut(s) 50, 72, 100, 405
BssAI RCCGGY 4 cut(s) 50, 72, 100, 405
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 2 cut(s) 41, 372
Bst4CI ACNGT 3 cut(s) 15, 472, 510
BstDEI CTNAG 2 cut(s) 38, 290
BstDSI CCRYGG 1 cut(s) 60
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 2 cut(s) 44, 375
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 2 cut(s) 41, 372
BstMCI CGRYCG 2 cut(s) 55, 73
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 423
BstZI CGGCCG 1 cut(s) 70
Bsu36I CCTNAGG 1 cut(s) 290
BsuRI GGCC 7 cut(s) 50, 59, 72, 90, 100, 261, 288
BtgI CCRYGG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 25
CfoI GCGC 1 cut(s) 495
Cfr10I RCCGGY 4 cut(s) 50, 72, 100, 405
Cfr13I GGNCC 3 cut(s) 57, 134, 286
Csp6I GTAC 2 cut(s) 16, 459
CspAI ACCGGT 1 cut(s) 405
CviAII CATG 2 cut(s) 353, 499
CviQI GTAC 2 cut(s) 16, 459
DdeI CTNAG 2 cut(s) 38, 290
DpnI GATC 2 cut(s) 43, 374
DpnII GATC 2 cut(s) 41, 372
DraIII CACNNNGTG 1 cut(s) 149
DriI GACNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 2 cut(s) 48, 70
EagI CGGCCG 1 cut(s) 70
Eam1105I GACNNNNNGTC 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 70
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 317
Eco81I CCTNAGG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 286
EcoRV GATATC 1 cut(s) 445
FaeI CATG 2 cut(s) 356, 502
FaiI YATR 9 cut(s) 312, 314, 322, 354, 395, 397, 440, 483, 500
FatI CATG 2 cut(s) 352, 498
FauNDI CATATG 1 cut(s) 395
Fnu4HI GCNGC 1 cut(s) 412
Fsp4HI GCNGC 1 cut(s) 412
FspBI CTAG 2 cut(s) 387, 477
FspI TGCGCA 1 cut(s) 494
GlaI GCGC 1 cut(s) 494
GluI GCNGC 1 cut(s) 412
HaeIII GGCC 7 cut(s) 50, 59, 72, 90, 100, 261, 288
HapII CCGG 4 cut(s) 51, 73, 101, 406
HhaI GCGC 1 cut(s) 495
Hin1II CATG 2 cut(s) 356, 502
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 1 cut(s) 435
HpaII CCGG 4 cut(s) 51, 73, 101, 406
HphI GGTGA 2 cut(s) 200, 439
Hpy188I TCNGA 1 cut(s) 372
Hpy188III TCNNGA 3 cut(s) 149, 178, 419
Hpy99I CGWCG 2 cut(s) 26, 431
HpyAV CCTTC 3 cut(s) 292, 332, 425
HpyCH4III ACNGT 3 cut(s) 15, 472, 510
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 2 cut(s) 324, 411
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 2 cut(s) 38, 290
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 2 cut(s) 356, 502
HspAI GCGC 1 cut(s) 493
Kzo9I GATC 2 cut(s) 41, 372
Lsp1109I GCAGC 1 cut(s) 423
MaeI CTAG 2 cut(s) 387, 477
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 112, 484
MalI GATC 2 cut(s) 43, 374
MbiI CCGCTC 1 cut(s) 175
MboI GATC 2 cut(s) 41, 372
MluCI AATT 1 cut(s) 315
MlyI GAGTC 1 cut(s) 429
MnlI CCTC 6 cut(s) 145, 184, 251, 258, 338, 341
MspI CCGG 4 cut(s) 51, 73, 101, 406
Mva1269I GAATGC 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeI CATATG 1 cut(s) 395
NdeII GATC 2 cut(s) 41, 372
NlaIII CATG 2 cut(s) 356, 502
NlaIV GGNNCC 2 cut(s) 158, 202
NmuCI GTSAC 1 cut(s) 112
NsbI TGCGCA 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 366
Pfl23II CGTACG 1 cut(s) 15
PinAI ACCGGT 1 cut(s) 405
PkrI GCNGC 1 cut(s) 413
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 158, 202
PspPI GGNCC 3 cut(s) 57, 134, 286
PspXI VCTCGAGB 1 cut(s) 329
RsaI GTAC 2 cut(s) 17, 460
RsaNI GTAC 2 cut(s) 16, 459
SatI GCNGC 1 cut(s) 412
Sau3AI GATC 2 cut(s) 41, 372
Sau96I GGNCC 3 cut(s) 57, 134, 286
SchI GAGTC 1 cut(s) 429
SetI ASST 8 cut(s) 27, 143, 195, 213, 276, 330, 343, 482
SfiI GGCCNNNNNGGCC 1 cut(s) 56
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 223, 329
SmoI CTYRAG 2 cut(s) 223, 329
Sse9I AATT 1 cut(s) 315
SsiI CCGC 1 cut(s) 173
SspMI CTAG 2 cut(s) 387, 477
TaaI ACNGT 3 cut(s) 15, 472, 510
TaiI ACGT 1 cut(s) 27
TaqI TCGA 2 cut(s) 330, 360
TasI AATT 1 cut(s) 315
TatI WGTACW 1 cut(s) 458
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 1 cut(s) 411
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 2 cut(s) 387, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.