FvH4_7g23800

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0011593
Physical Location & Seq
Forward (+)
18491822 .. 18495102
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23800.t1

Sequence Viewer

Length: 1608 bp
ATGACAAGGAGACGGTTGTTTGAGGCCACAGAACATCAACATGGTGCCATTAACACCATGTTTTTCAGCAGCAGAATGCTGAAGTTGGCAAAGCTTCTGAGGAGAGGAAGACAGGTTTTCTTCGTTTTGTTGCTGCTAGGTTTCTTGTCAGTGATGGCAAAGTTTGCTTTGCAGCTGAATGCATTTCAGGAAATAAGGATGAGGGAACGGATAGGTTACATTCGACGACCTTCGACAACTTTGCAACATAACATGGTTAGTAGTGAAGGTGACAAGGGTTATAATGTGCCTCTGAAACAACGAATAAGTTCCCGTCAGACTGCTGATATCTGGACACAACCAAACAGTGAAAACTACTACAAATGCATTAATCGAGAAAAGAAGGAAATATTGCAAGAAAATGCAACAAATGGCTATATTATGATCAAGGCCAATGGTGGGTTGAATCAAATGAGGCTTGGGATAAGTGATATGGTTGCCGTAGCAAAACTTATGAATGCCACTCTTGTTCTCCCTTCTTTAGATCATAAATCCTTTTGGACTGATCCAAGTGATTTTAAGGACATTTTCAACTGGAAGAATTTCATGGAAGTCTTAAAAGACGATATCATGATTGTAGAGTCACTTCCGGCGGAGCTTGCAGACGTGAAGCCTCTCCTCAGGGCTCCGGTTTCCTGGTCCAAGACTAGTTACTACAGAGGATATATCTCTACGTTATTGAAGCAGCACAAAGTGCTCAAATTCACTCATACAGATTCCAGACTAGCTAATAATCTCGGTGGTTCAACCCAAAGACTACGCTGCCGAGCAATGTTTGAGGCACTCAGATTCTCACCTGCAATAGAACAGCTTGCAACGAAGTTGGTTAGCAGATTAAAGAACAACAGTAGATACATTGCTCTCCACTTAAGATATGAAAAGGACATGCTTTCTTTCACAGGCTGCAGTCACAATCTGACCAAAGAGGAAGATAGAGAACTTACAAAGCAAAGGAAAGAAACATCTCACTGGAAGCGTAAGAAGATCAATGGCACCCATCATAGACTCCAAGGGGTATGCCCCATGACCCCAAGAGAGGTTGCTGTGTTTCTAGAGGCAATTGGGTTTCCTTTGGATACACAGGTTTATGTAGTATCTGGGAAGATCTACGGGAGAGATGGGATTACACCTCTGAAAAATAAGTACCCCAATTTATTCTTCCACTCAAATTTAGCCACAGAAGAAGAGCTGCAACCTTTCAAGAACTTGCACAACCAGCTTGCTGCATTGGACTACATTGTGGCTTTGGAGAGTGATGTGTTCATTTATTCTTTTGAAGGAAATATGGCTAAAGCAGTCAAAGGACATCGACGATTTGAAAGGTTTCGGAAGACGATCAACCCCAACAAGCAACAATTTGTGGCATTGATTGATAGGTTGGACAAGGGACTGATATCTTGGGATAAATTCTCATCCAAGGTGAAGAAGTTGCATGAAAATAGGATCGGAGCACCATACTTGAGAATAGCCACAGGTAGCTATAAAAATGAGGAAAACTTTTACTCAAACCCTTTCCCGGGTTGCATCTGTGAAAAGTCCGGAGGCTCATCAATAGTAGCCAATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

536

Amino Acids

61.73

Weight (kDa)

9.83

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 139 - 452 3.4e-97 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
AccB1I GGYRCC 2 cut(s) 44, 1031
AccIII TCCGGA 1 cut(s) 1577
AciI CCGC 1 cut(s) 632
AclWI GGATC 2 cut(s) 539, 1490
AcsI RAATTY 4 cut(s) 580, 740, 1207, 1445
AcuI CTGAAG 1 cut(s) 101
AdeI CACNNNGTG 1 cut(s) 733
AfaI GTAC 1 cut(s) 1184
AfiI CCNNNNNNNGG 4 cut(s) 438, 1075, 1555, 1556
AflII CTTAAG 1 cut(s) 907
AgsI TTSAA 7 cut(s) 445, 571, 721, 786, 1240, 1316, 1358
AhlI ACTAGT 1 cut(s) 686
AjiI CACGTC 1 cut(s) 646
AjnI CCWGG 1 cut(s) 674
AjuI GAANNNNNNNTTGG 2 cut(s) 1181, 1213
AluBI AGCT 8 cut(s) 94, 175, 637, 767, 850, 1228, 1258, 1518
AluI AGCT 8 cut(s) 94, 175, 637, 767, 850, 1228, 1258, 1518
Alw21I GWGCWC 2 cut(s) 738, 1492
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 2 cut(s) 539, 1490
Ama87I CYCGRG 1 cut(s) 1555
Aor13HI TCCGGA 1 cut(s) 1577
AoxI GGCC 2 cut(s) 24, 429
ApeKI GCWGC 8 cut(s) 69, 133, 172, 724, 801, 942, 1228, 1262
ApoI RAATTY 4 cut(s) 580, 740, 1207, 1445
AseI ATTAAT 1 cut(s) 369
Asp700I GAANNNNTTC 3 cut(s) 307, 581, 1362
AspS9I GGNCC 1 cut(s) 678
AsuC2I CCSGG 2 cut(s) 1556, 1557
AsuHPI GGTGA 3 cut(s) 281, 825, 1471
AvaI CYCGRG 1 cut(s) 1555
AvaII GGWCC 1 cut(s) 678
AxyI CCTNAGG 1 cut(s) 659
BaeI ACNNNNGTAYC 2 cut(s) 1107, 1140
BanI GGYRCC 2 cut(s) 44, 1031
BanII GRGCYC 1 cut(s) 667
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
BbsI GAAGAC 2 cut(s) 115, 1376
Bbv12I GWGCWC 2 cut(s) 738, 1492
BbvI GCAGC 8 cut(s) 81, 120, 184, 736, 788, 929, 1215, 1249
BccI CCATC 3 cut(s) 148, 1044, 1151
BceAI ACGGC 1 cut(s) 464
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 676
BciVI GTATCC 1 cut(s) 1108
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 2 cut(s) 1556, 1557
BcoDI GTCTC 1 cut(s) 4
BcuI ACTAGT 1 cut(s) 686
BfaI CTAG 4 cut(s) 137, 687, 764, 1091
BfmI CTRYAG 2 cut(s) 694, 943
BfrI CTTAAG 1 cut(s) 907
BfuAI ACCTGC 1 cut(s) 844
BfuI GTATCC 1 cut(s) 1108
BglII AGATCT 1 cut(s) 1143
BisI GCNGC 8 cut(s) 70, 134, 173, 725, 802, 943, 1229, 1263
BlsI GCNGC 8 cut(s) 71, 135, 174, 726, 803, 944, 1230, 1264
Bme1390I CCNGG 3 cut(s) 676, 1556, 1557
Bme18I GGWCC 1 cut(s) 678
BmeT110I CYCGRG 1 cut(s) 1555
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 678
BmiI GGNNCC 3 cut(s) 46, 666, 1033
BmrFI CCNGG 3 cut(s) 676, 1556, 1557
BmsI GCATC 1 cut(s) 1572
BpiI GAAGAC 2 cut(s) 115, 1376
BpuEI CTTGAG 1 cut(s) 1519
BpuMI CCSGG 2 cut(s) 1556, 1557
BsaJI CCNNGG 3 cut(s) 1048, 1455, 1555
BsaWI WCCGGW 2 cut(s) 667, 1577
Bsc4I CCNNNNNNNGG 4 cut(s) 438, 1075, 1555, 1556
Bse1I ACTGG 2 cut(s) 578, 1013
Bse21I CCTNAGG 1 cut(s) 659
Bse3DI GCAATG 2 cut(s) 816, 894
BseAI TCCGGA 1 cut(s) 1577
BseBI CCWGG 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 1048, 1455, 1555
BseGI GGATG 2 cut(s) 204, 1451
BseLI CCNNNNNNNGG 4 cut(s) 438, 1075, 1555, 1556
BseMI GCAATG 2 cut(s) 816, 894
BseMII CTCAG 3 cut(s) 89, 673, 838
BseNI ACTGG 2 cut(s) 578, 1013
BseRI GAGGAG 2 cut(s) 115, 647
BseXI GCAGC 8 cut(s) 81, 120, 184, 736, 788, 929, 1215, 1249
BshFI GGCC 2 cut(s) 26, 431
BshNI GGYRCC 2 cut(s) 44, 1031
BsiHKAI GWGCWC 2 cut(s) 738, 1492
BsiHKCI CYCGRG 1 cut(s) 1555
BsiSI CCGG 4 cut(s) 629, 668, 1556, 1578
BslFI GGGAC 1 cut(s) 1440
BslI CCNNNNNNNGG 4 cut(s) 438, 1075, 1555, 1556
BsmAI GTCTC 1 cut(s) 4
BsmBI CGTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 1440
BsmI GAATGC 3 cut(s) 81, 184, 502
BsnI GGCC 2 cut(s) 26, 431
BsoBI CYCGRG 1 cut(s) 1555
Bsp1286I GDGCHC 3 cut(s) 667, 738, 1492
Bsp13I TCCGGA 1 cut(s) 1577
Bsp143I GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
BspACI CCGC 1 cut(s) 632
BspANI GGCC 2 cut(s) 26, 431
BspCNI CTCAG 3 cut(s) 90, 672, 837
BspEI TCCGGA 1 cut(s) 1577
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 3 cut(s) 46, 666, 1033
BspMAI CTGCAG 1 cut(s) 947
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 2 cut(s) 539, 1490
BspQI GCTCTTC 1 cut(s) 1218
BspT107I GGYRCC 2 cut(s) 44, 1031
BspTI CTTAAG 1 cut(s) 907
BsrDI GCAATG 2 cut(s) 816, 894
BsrI ACTGG 2 cut(s) 578, 1013
BssECI CCNNGG 3 cut(s) 1048, 1455, 1555
BssMI GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
BssT1I CCWWGG 2 cut(s) 1048, 1455
Bst2UI CCWGG 1 cut(s) 676
Bst4CI ACNGT 3 cut(s) 15, 347, 887
Bst6I CTCTTC 1 cut(s) 1218
BstAFI CTTAAG 1 cut(s) 907
BstAPI GCANNNNNTGC 2 cut(s) 164, 733
BstC8I GCNNGC 3 cut(s) 639, 852, 1260
BstDEI CTNAG 3 cut(s) 98, 659, 824
BstF5I GGATG 2 cut(s) 204, 1451
BstKTI GATC 7 cut(s) 426, 526, 547, 1026, 1146, 1377, 1485
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
BstMWI GCNNNNNNNGC 4 cut(s) 164, 638, 733, 1255
BstNI CCWGG 1 cut(s) 676
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 3 cut(s) 674, 1554, 1555
BstSFI CTRYAG 2 cut(s) 694, 943
BstV1I GCAGC 8 cut(s) 81, 120, 184, 736, 788, 929, 1215, 1249
BstV2I GAAGAC 2 cut(s) 115, 1376
BstX2I RGATCY 1 cut(s) 1143
BstYI RGATCY 1 cut(s) 1143
Bsu36I CCTNAGG 1 cut(s) 659
BsuI GTATCC 1 cut(s) 1108
BsuRI GGCC 2 cut(s) 26, 431
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 2 cut(s) 204, 1451
BtsIMutI CAGTG 3 cut(s) 156, 352, 1006
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 3 cut(s) 639, 852, 1260
CciI TCATGA 1 cut(s) 609
Cfr13I GGNCC 1 cut(s) 678
Cfr9I CCCGGG 1 cut(s) 1555
Csp6I GTAC 1 cut(s) 1183
CviAII CATG 8 cut(s) 41, 58, 253, 586, 610, 925, 1063, 1472
CviQI GTAC 1 cut(s) 1183
DdeI CTNAG 3 cut(s) 98, 659, 824
DpnI GATC 7 cut(s) 425, 525, 546, 1025, 1145, 1376, 1484
DpnII GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
DraIII CACNNNGTG 1 cut(s) 733
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
EciI GGCGGA 1 cut(s) 647
Eco130I CCWWGG 2 cut(s) 1048, 1455
Eco24I GRGCYC 1 cut(s) 667
Eco32I GATATC 3 cut(s) 328, 607, 1434
Eco47I GGWCC 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 101
Eco81I CCTNAGG 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 1555
EcoRII CCWGG 1 cut(s) 674
EcoRV GATATC 3 cut(s) 328, 607, 1434
EcoT14I CCWWGG 2 cut(s) 1048, 1455
EcoT22I ATGCAT 2 cut(s) 184, 368
EcoT38I GRGCYC 1 cut(s) 667
ErhI CCWWGG 2 cut(s) 1048, 1455
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 8 cut(s) 44, 61, 256, 589, 613, 928, 1066, 1475
FaqI GGGAC 1 cut(s) 1440
FatI CATG 8 cut(s) 40, 57, 252, 585, 609, 924, 1062, 1471
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 8 cut(s) 70, 134, 173, 725, 802, 943, 1229, 1263
FokI GGATG 2 cut(s) 211, 1438
FriOI GRGCYC 1 cut(s) 667
Fsp4HI GCNGC 8 cut(s) 70, 134, 173, 725, 802, 943, 1229, 1263
FspBI CTAG 4 cut(s) 137, 687, 764, 1091
GluI GCNGC 8 cut(s) 70, 134, 173, 725, 802, 943, 1229, 1263
HaeIII GGCC 2 cut(s) 26, 431
HapII CCGG 4 cut(s) 629, 668, 1556, 1578
Hin1II CATG 8 cut(s) 44, 61, 256, 589, 613, 928, 1066, 1475
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 5 cut(s) 445, 620, 755, 828, 1044
HpaII CCGG 4 cut(s) 629, 668, 1556, 1578
HphI GGTGA 3 cut(s) 281, 825, 1471
Hpy188I TCNGA 8 cut(s) 99, 294, 318, 827, 957, 1173, 1368, 1487
Hpy188III TCNNGA 8 cut(s) 188, 331, 374, 610, 759, 1091, 1240, 1578
Hpy99I CGWCG 2 cut(s) 228, 1353
HpyAV CCTTC 5 cut(s) 240, 260, 376, 525, 1310
HpyCH4III ACNGT 3 cut(s) 15, 347, 887
HpyCH4IV ACGT 2 cut(s) 645, 713
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 638, 733, 1255
HpyF3I CTNAG 3 cut(s) 98, 659, 824
HpySE526I ACGT 2 cut(s) 645, 713
Hsp92II CATG 8 cut(s) 44, 61, 256, 589, 613, 928, 1066, 1475
Kpn2I TCCGGA 1 cut(s) 1577
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
LguI GCTCTTC 1 cut(s) 1218
LmnI GCTCC 3 cut(s) 634, 670, 1487
Lsp1109I GCAGC 8 cut(s) 81, 120, 184, 736, 788, 929, 1215, 1249
LweI GCATC 1 cut(s) 1572
MaeI CTAG 4 cut(s) 137, 687, 764, 1091
MaeII ACGT 2 cut(s) 645, 713
MaeIII GTNAC 5 cut(s) 215, 269, 621, 689, 947
MalI GATC 7 cut(s) 425, 525, 546, 1025, 1145, 1376, 1484
MboI GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
MfeI CAATTG 1 cut(s) 1098
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 3 cut(s) 667, 738, 1492
MluCI AATT 7 cut(s) 580, 740, 1098, 1189, 1207, 1394, 1445
MlyI GAGTC 2 cut(s) 629, 1038
MmeI TCCRAC 1 cut(s) 1398
Mph1103I ATGCAT 2 cut(s) 184, 368
MroI TCCGGA 1 cut(s) 1577
MroXI GAANNNNTTC 3 cut(s) 307, 581, 1362
MseI TTAA 6 cut(s) 51, 369, 558, 596, 875, 908
MslI CAYNNNNRTG 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 907
MspI CCGG 4 cut(s) 629, 668, 1556, 1578
MspR9I CCNGG 3 cut(s) 676, 1556, 1557
MunI CAATTG 1 cut(s) 1098
Mva1269I GAATGC 3 cut(s) 81, 184, 502
MvaI CCWGG 1 cut(s) 676
MwoI GCNNNNNNNGC 4 cut(s) 164, 638, 733, 1255
NciI CCSGG 2 cut(s) 1556, 1557
NdeII GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
NlaIII CATG 8 cut(s) 44, 61, 256, 589, 613, 928, 1066, 1475
NlaIV GGNNCC 3 cut(s) 46, 666, 1033
NmeAIII GCCGAG 1 cut(s) 830
NmuCI GTSAC 3 cut(s) 269, 621, 947
NsiI ATGCAT 2 cut(s) 184, 368
NspI RCATGY 1 cut(s) 928
PagI TCATGA 1 cut(s) 609
PaqCI CACCTGC 1 cut(s) 844
PciSI GCTCTTC 1 cut(s) 1218
PctI GAATGC 3 cut(s) 81, 184, 502
PdmI GAANNNNTTC 3 cut(s) 307, 581, 1362
PfeI GAWTC 3 cut(s) 445, 755, 828
PkrI GCNGC 8 cut(s) 71, 135, 174, 726, 803, 944, 1230, 1264
PleI GAGTC 2 cut(s) 628, 1038
PpsI GAGTC 2 cut(s) 628, 1038
PshBI ATTAAT 1 cut(s) 369
PsiI TTATAA 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 674
PspGI CCWGG 1 cut(s) 674
PspN4I GGNNCC 3 cut(s) 46, 666, 1033
PspPI GGNCC 1 cut(s) 678
PstI CTGCAG 1 cut(s) 947
PsuI RGATCY 1 cut(s) 1143
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 1 cut(s) 1184
RsaNI GTAC 1 cut(s) 1183
RseI CAYNNNNRTG 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 1218
SaqAI TTAA 6 cut(s) 51, 369, 558, 596, 875, 908
SatI GCNGC 8 cut(s) 70, 134, 173, 725, 802, 943, 1229, 1263
Sau3AI GATC 7 cut(s) 423, 523, 544, 1023, 1143, 1374, 1482
Sau96I GGNCC 1 cut(s) 678
SchI GAGTC 2 cut(s) 629, 1038
ScrFI CCNGG 3 cut(s) 676, 1556, 1557
SduI GDGCHC 3 cut(s) 667, 738, 1492
SfaNI GCATC 1 cut(s) 1572
SfcI CTRYAG 2 cut(s) 694, 943
SinI GGWCC 1 cut(s) 678
SmaI CCCGGG 1 cut(s) 1557
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 907, 1498
SmoI CTYRAG 2 cut(s) 907, 1498
SpeI ACTAGT 1 cut(s) 686
Sse9I AATT 7 cut(s) 580, 740, 1098, 1189, 1207, 1394, 1445
SsiI CCGC 1 cut(s) 632
SspI AATATT 1 cut(s) 390
SspMI CTAG 4 cut(s) 137, 687, 764, 1091
StyD4I CCNGG 3 cut(s) 674, 1554, 1555
StyI CCWWGG 2 cut(s) 1048, 1455
TaaI ACNGT 3 cut(s) 15, 347, 887
TaiI ACGT 2 cut(s) 648, 716
TaqI TCGA 4 cut(s) 223, 233, 373, 1348
TasI AATT 7 cut(s) 580, 740, 1098, 1189, 1207, 1394, 1445
TfiI GAWTC 3 cut(s) 445, 755, 828
Tru1I TTAA 6 cut(s) 51, 369, 558, 596, 875, 908
Tru9I TTAA 6 cut(s) 51, 369, 558, 596, 875, 908
TscAI CASTG 3 cut(s) 156, 352, 1013
TseFI GTSAC 3 cut(s) 269, 621, 947
TseI GCWGC 8 cut(s) 69, 133, 172, 724, 801, 942, 1228, 1262
Tsp45I GTSAC 3 cut(s) 269, 621, 947
TspDTI ATGAA 5 cut(s) 509, 574, 930, 1291, 1488
TspGWI ACGGA 1 cut(s) 223
TspMI CCCGGG 1 cut(s) 1555
TspRI CASTG 3 cut(s) 156, 352, 1013
Vha464I CTTAAG 1 cut(s) 907
VpaK11BI GGWCC 1 cut(s) 678
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 4 cut(s) 580, 740, 1207, 1445
XbaI TCTAGA 1 cut(s) 1090
XceI RCATGY 1 cut(s) 928
XmaI CCCGGG 1 cut(s) 1555
XmnI GAANNNNTTC 3 cut(s) 307, 581, 1362
XspI CTAG 4 cut(s) 137, 687, 764, 1091
Zsp2I ATGCAT 2 cut(s) 184, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.