Rroxscaffold_4G00287920

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011593
Physical Location & Seq
Reverse (-)
8817573 .. 8820231
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287920.1

Sequence Viewer

Length: 1608 bp
ATGACAAGGAGACGGTTATTTGAGGCCACAGAAGATCAACAAGATGCCATTAACAGCATGTTCTTCAGCAGCAGACCGCTCAAGTTGGCAAAGCTTTTGATGAGAGGAAGACGGGTTTTCGTGGTTTTGTTGCTGTTAGGTTTCGTGTCAGTGATGACAAAATTTGCTTTACAGCTGAATGCATTACAGGAATTGAGAATGAGGGAACGAATAGCTTTTATTCGACGACCTTCGACAGTTTTGCAACATAAAATGGTTAGTGAAGGTGACGAGGGTTATAATGTGGCTCTGAAACAACGAAGTTCCAGTCAGACAGGTGATATCTGGACGCAACCAAACAGTGAAAACTACTACAAATGTATTAATCGAGCAAAGAAGGATATATTGAAACGAAATGCAACAAATGGCTATATTATGATCAAGGCCAACGGTGGGTTGAATCAAATGAAGCTTGGGATAAGTGATATGGTTGCCGTAGCAAAACTTATGAATGCCACTCTGGTTCTCCCTTTTTTAGATCATAAATCCTTTTGGACGGATCCAAGTGATTTTAAGGACATTTTCAACTGGAAGAATTTCATGGAAGTCTTAAAAGATGATATCATGGTTATAGAGTCACTCCCACCGGAGCTTGCAGACCTGAAGCCTCTCCCGAGGGCTCCAGTTTCCTGGTCCAAGGCTAGTTACTACAGAGGATATATGTCTTCGTTATTGAAGCAGCACAAAGTGCTCAAATTCACGTACACAGATTCCAGACTTGCTAATGATCTTGGTGGTTCATTCCAAAAACTACGCTGCCGTGCAATGTTTGAGGCACTGAGATTCTCACCTGAAATCGAACAACTAGCATCAAAGTTGGTTAACAGATTAAAGAACAACAATAGTAGATACATTGCTCTCCATTTAAGATATGAAAAGGACATGCTTTCTTTCACAGGCTGCAGTCACAATCTGACCAAAGACGAAGATAGAGAACTCGCAAAAATAAGGAAACAAACATCTCACTGGAAGCATAAGACGATTAATAGCACCAATCATAGACTCCAAGGGGTATGCCCCATGACCCCAAGAGAGGCAGCTGTGTTTCTTGAAGCAATTGGGTTTCCTTTGGATACAAAAGTTTATGTAGCCGCTGGGAAGATCTACGGGAGAGATGGGATTAGACCTCTGAAAGATAAGTACCCCAATTTATTCTTCCACTCAAATTTAGCGACTGAAGTAGAGTTGCAACCTTTCAAGAACTTGCAGAACCAGCTTGCTGCCTTGGATTACATTGTGGCTTTGGAGAGTGATGTGTTCATTTATTCTTTTGATGGAAATATGGCTAAAGCAATAAAAGGGCATCGACGATTTGAAGGGTTCCGGAAGACGATCAACCCCAACAAACAACAATTTGTGACATTGATTGATAGGTTGGACAACGGAATGATATCTTGGGAAAAATTCTCATCCAAGGTGAAGAAGTTGCATGAAAATCGGATCGGAGCACCATACTCCAGAGTAGCCAGAGGTGACCACAAACTTGAGGAGAACTTTTACGCAAACCCTTTTCCGGGTTGCATCTGTGAAAAGTCCGGAGGCTCATCAACAGTAGACAAGCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

535

Amino Acids

61.53

Weight (kDa)

9.72

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 137 - 451 3.2e-97 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 79
AccI GTMKAC 1 cut(s) 1593
AccIII TCCGGA 2 cut(s) 1362, 1574
AciI CCGC 2 cut(s) 77, 1131
AclWI GGATC 3 cut(s) 533, 546, 1487
AcsI RAATTY 5 cut(s) 161, 574, 734, 1204, 1442
AcuI CTGAAG 3 cut(s) 49, 662, 1236
AdeI CACNNNGTG 1 cut(s) 727
AfaI GTAC 2 cut(s) 743, 1181
AfiI CCNNNNNNNGG 4 cut(s) 432, 1072, 1552, 1553
AgsI TTSAA 7 cut(s) 388, 439, 565, 715, 1091, 1237, 1355
AjnI CCWGG 1 cut(s) 668
AjuI GAANNNNNNNTTGG 4 cut(s) 1178, 1210, 1417, 1449
AluBI AGCT 7 cut(s) 94, 175, 215, 451, 631, 1079, 1255
AluI AGCT 7 cut(s) 94, 175, 215, 451, 631, 1079, 1255
Alw21I GWGCWC 2 cut(s) 732, 1489
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 3 cut(s) 533, 546, 1487
Ama87I CYCGRG 1 cut(s) 652
Aor13HI TCCGGA 2 cut(s) 1362, 1574
AoxI GGCC 2 cut(s) 24, 423
ApeKI GCWGC 6 cut(s) 69, 718, 795, 939, 1076, 1259
ApoI RAATTY 5 cut(s) 161, 574, 734, 1204, 1442
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
AseI ATTAAT 2 cut(s) 363, 1023
Asp700I GAANNNNTTC 1 cut(s) 575
AspS9I GGNCC 1 cut(s) 672
AsuC2I CCSGG 1 cut(s) 1554
AsuHPI GGTGA 5 cut(s) 278, 329, 819, 1468, 1523
AvaI CYCGRG 1 cut(s) 652
AvaII GGWCC 1 cut(s) 672
BaeI ACNNNNGTAYC 2 cut(s) 1104, 1137
BamHI GGATCC 1 cut(s) 538
BanII GRGCYC 1 cut(s) 661
BbsI GAAGAC 3 cut(s) 115, 696, 1373
Bbv12I GWGCWC 2 cut(s) 732, 1489
BbvI GCAGC 6 cut(s) 81, 730, 782, 926, 1088, 1246
BccI CCATC 2 cut(s) 1148, 1307
BceAI ACGGC 2 cut(s) 458, 783
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 1457, 1491
BciT130I CCWGG 1 cut(s) 670
BciVI GTATCC 1 cut(s) 1105
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 1 cut(s) 1554
BcoDI GTCTC 1 cut(s) 4
BfaI CTAG 2 cut(s) 681, 845
BfmI CTRYAG 2 cut(s) 688, 940
BfuI GTATCC 1 cut(s) 1105
BglII AGATCT 1 cut(s) 1140
BisI GCNGC 7 cut(s) 70, 719, 796, 940, 1077, 1131, 1260
BlsI GCNGC 7 cut(s) 71, 720, 797, 941, 1078, 1132, 1261
Bme1390I CCNGG 2 cut(s) 670, 1554
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 652
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 3 cut(s) 540, 660, 1361
BmrFI CCNGG 2 cut(s) 670, 1554
BmsI GCATC 4 cut(s) 34, 857, 1351, 1569
BpiI GAAGAC 3 cut(s) 115, 696, 1373
BpmI CTGGAG 2 cut(s) 645, 1480
BpuEI CTTGAG 2 cut(s) 65, 1544
BpuMI CCSGG 1 cut(s) 1554
BsaAI YACGTR 1 cut(s) 741
BsaJI CCNNGG 5 cut(s) 653, 675, 1045, 1263, 1452
BsaWI WCCGGW 3 cut(s) 625, 1362, 1574
Bsc4I CCNNNNNNNGG 4 cut(s) 432, 1072, 1552, 1553
Bse1I ACTGG 4 cut(s) 306, 572, 662, 1010
Bse3DI GCAATG 2 cut(s) 810, 891
BseAI TCCGGA 2 cut(s) 1362, 1574
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 5 cut(s) 653, 675, 1045, 1263, 1452
BseGI GGATG 1 cut(s) 1448
BseLI CCNNNNNNNGG 4 cut(s) 432, 1072, 1552, 1553
BseMI GCAATG 2 cut(s) 810, 891
BseMII CTCAG 1 cut(s) 809
BseNI ACTGG 4 cut(s) 306, 572, 662, 1010
BseRI GAGGAG 1 cut(s) 1541
BseXI GCAGC 6 cut(s) 81, 730, 782, 926, 1088, 1246
BseYI CCCAGC 1 cut(s) 1133
BshFI GGCC 2 cut(s) 26, 425
BsiHKAI GWGCWC 2 cut(s) 732, 1489
BsiHKCI CYCGRG 1 cut(s) 652
BsiSI CCGG 4 cut(s) 626, 1363, 1553, 1575
BslI CCNNNNNNNGG 4 cut(s) 432, 1072, 1552, 1553
BsmAI GTCTC 1 cut(s) 4
BsmBI CGTCTC 1 cut(s) 4
BsmI GAATGC 2 cut(s) 184, 496
BsnI GGCC 2 cut(s) 26, 425
BsoBI CYCGRG 1 cut(s) 652
Bsp1286I GDGCHC 3 cut(s) 661, 732, 1489
Bsp13I TCCGGA 2 cut(s) 1362, 1574
Bsp143I GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
BspACI CCGC 2 cut(s) 77, 1131
BspANI GGCC 2 cut(s) 26, 425
BspCNI CTCAG 1 cut(s) 810
BspEI TCCGGA 2 cut(s) 1362, 1574
BspLI GGNNCC 3 cut(s) 540, 660, 1361
BspMAI CTGCAG 1 cut(s) 944
BspPI GGATC 3 cut(s) 533, 546, 1487
BsrBI CCGCTC 1 cut(s) 79
BsrDI GCAATG 2 cut(s) 810, 891
BsrI ACTGG 4 cut(s) 306, 572, 662, 1010
BssECI CCNNGG 5 cut(s) 653, 675, 1045, 1263, 1452
BssMI GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
BssT1I CCWWGG 4 cut(s) 675, 1045, 1263, 1452
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 5 cut(s) 15, 238, 341, 431, 1591
BstAPI GCANNNNNTGC 1 cut(s) 727
BstBAI YACGTR 1 cut(s) 741
BstC8I GCNNGC 2 cut(s) 633, 1257
BstDEI CTNAG 1 cut(s) 818
BstEII GGTNACC 1 cut(s) 1511
BstF5I GGATG 1 cut(s) 1448
BstKTI GATC 8 cut(s) 37, 420, 520, 541, 769, 1143, 1374, 1482
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
BstMWI GCNNNNNNNGC 2 cut(s) 727, 1252
BstNI CCWGG 1 cut(s) 670
BstNSI RCATGY 2 cut(s) 61, 925
BstPI GGTNACC 1 cut(s) 1511
BstSCI CCNGG 2 cut(s) 668, 1552
BstSFI CTRYAG 2 cut(s) 688, 940
BstV1I GCAGC 6 cut(s) 81, 730, 782, 926, 1088, 1246
BstV2I GAAGAC 3 cut(s) 115, 696, 1373
BstX2I RGATCY 2 cut(s) 538, 1140
BstXI CCANNNNNNTGG 1 cut(s) 669
BstYI RGATCY 2 cut(s) 538, 1140
BsuI GTATCC 1 cut(s) 1105
BsuRI GGCC 2 cut(s) 26, 425
BtsCI GGATG 1 cut(s) 1448
BtsIMutI CAGTG 4 cut(s) 156, 346, 815, 1003
Cac8I GCNNGC 2 cut(s) 633, 1257
Cfr13I GGNCC 1 cut(s) 672
CseI GACGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 742, 1180
CviAII CATG 6 cut(s) 58, 580, 604, 922, 1060, 1469
CviQI GTAC 2 cut(s) 742, 1180
DdeI CTNAG 1 cut(s) 818
DpnI GATC 8 cut(s) 36, 419, 519, 540, 768, 1142, 1373, 1481
DpnII GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
DraIII CACNNNGTG 1 cut(s) 727
Eco130I CCWWGG 4 cut(s) 675, 1045, 1263, 1452
Eco24I GRGCYC 1 cut(s) 661
Eco32I GATATC 3 cut(s) 322, 601, 1431
Eco47I GGWCC 1 cut(s) 672
Eco57I CTGAAG 3 cut(s) 49, 662, 1236
Eco88I CYCGRG 1 cut(s) 652
Eco91I GGTNACC 1 cut(s) 1511
EcoO65I GGTNACC 1 cut(s) 1511
EcoRII CCWGG 1 cut(s) 668
EcoRV GATATC 3 cut(s) 322, 601, 1431
EcoT14I CCWWGG 4 cut(s) 675, 1045, 1263, 1452
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 4 cut(s) 675, 1045, 1263, 1452
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 6 cut(s) 61, 583, 607, 925, 1063, 1472
FatI CATG 6 cut(s) 57, 579, 603, 921, 1059, 1468
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 1 cut(s) 1593
Fnu4HI GCNGC 7 cut(s) 70, 719, 796, 940, 1077, 1131, 1260
FokI GGATG 1 cut(s) 1435
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 7 cut(s) 70, 719, 796, 940, 1077, 1131, 1260
FspBI CTAG 2 cut(s) 681, 845
GluI GCNGC 7 cut(s) 70, 719, 796, 940, 1077, 1131, 1260
GsaI CCCAGC 1 cut(s) 1137
GsuI CTGGAG 2 cut(s) 645, 1480
HaeIII GGCC 2 cut(s) 26, 425
HapII CCGG 4 cut(s) 626, 1363, 1553, 1575
HgaI GACGC 1 cut(s) 337
Hin1II CATG 6 cut(s) 61, 583, 607, 925, 1063, 1472
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 2 cut(s) 92, 449
HinfI GANTC 5 cut(s) 439, 614, 749, 822, 1041
HpaI GTTAAC 1 cut(s) 862
HpaII CCGG 4 cut(s) 626, 1363, 1553, 1575
HphI GGTGA 5 cut(s) 278, 329, 819, 1468, 1523
Hpy166II GTNNAC 3 cut(s) 744, 862, 1594
Hpy188I TCNGA 6 cut(s) 291, 312, 954, 1170, 1479, 1484
Hpy188III TCNNGA 8 cut(s) 325, 652, 753, 1088, 1237, 1363, 1497, 1575
Hpy8I GTNNAC 3 cut(s) 744, 862, 1594
Hpy99I CGWCG 2 cut(s) 228, 1350
HpyAV CCTTC 4 cut(s) 240, 257, 370, 1349
HpyCH4III ACNGT 5 cut(s) 15, 238, 341, 431, 1591
HpyCH4IV ACGT 1 cut(s) 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 727, 1252
HpyF3I CTNAG 1 cut(s) 818
HpySE526I ACGT 1 cut(s) 740
Hsp92II CATG 6 cut(s) 61, 583, 607, 925, 1063, 1472
Kpn2I TCCGGA 2 cut(s) 1362, 1574
Ksp22I TGATCA 1 cut(s) 417
KspAI GTTAAC 1 cut(s) 862
Kzo9I GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
LmnI GCTCC 3 cut(s) 628, 664, 1484
Lsp1109I GCAGC 6 cut(s) 81, 730, 782, 926, 1088, 1246
LweI GCATC 4 cut(s) 34, 857, 1351, 1569
MaeI CTAG 2 cut(s) 681, 845
MaeII ACGT 1 cut(s) 740
MaeIII GTNAC 6 cut(s) 266, 615, 683, 944, 1396, 1511
MalI GATC 8 cut(s) 36, 419, 519, 540, 768, 1142, 1373, 1481
MbiI CCGCTC 1 cut(s) 79
MboI GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
MfeI CAATTG 1 cut(s) 1095
MflI RGATCY 2 cut(s) 538, 1140
MhlI GDGCHC 3 cut(s) 661, 732, 1489
MluCI AATT 9 cut(s) 161, 191, 574, 734, 1095, 1186, 1204, 1391, 1442
MlyI GAGTC 2 cut(s) 623, 1035
MmeI TCCRAC 1 cut(s) 1395
Mph1103I ATGCAT 1 cut(s) 184
MroI TCCGGA 2 cut(s) 1362, 1574
MroXI GAANNNNTTC 1 cut(s) 575
MseI TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
MspA1I CMGCKG 3 cut(s) 175, 1079, 1133
MspI CCGG 4 cut(s) 626, 1363, 1553, 1575
MspR9I CCNGG 2 cut(s) 670, 1554
MunI CAATTG 1 cut(s) 1095
Mva1269I GAATGC 2 cut(s) 184, 496
MvaI CCWGG 1 cut(s) 670
MwoI GCNNNNNNNGC 2 cut(s) 727, 1252
NciI CCSGG 1 cut(s) 1554
NdeII GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
NlaIII CATG 6 cut(s) 61, 583, 607, 925, 1063, 1472
NlaIV GGNNCC 3 cut(s) 540, 660, 1361
NmuCI GTSAC 5 cut(s) 266, 615, 944, 1396, 1511
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 2 cut(s) 61, 925
PctI GAATGC 2 cut(s) 184, 496
PdmI GAANNNNTTC 1 cut(s) 575
PfeI GAWTC 3 cut(s) 439, 749, 822
PkrI GCNGC 7 cut(s) 71, 720, 797, 941, 1078, 1132, 1261
PleI GAGTC 2 cut(s) 622, 1035
PpsI GAGTC 2 cut(s) 622, 1035
Ppu21I YACGTR 1 cut(s) 741
PshBI ATTAAT 2 cut(s) 363, 1023
PsiI TTATAA 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 668
PspEI GGTNACC 1 cut(s) 1511
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 3 cut(s) 540, 660, 1361
PspPI GGNCC 1 cut(s) 672
PstI CTGCAG 1 cut(s) 944
PsuI RGATCY 2 cut(s) 538, 1140
PvuII CAGCTG 2 cut(s) 175, 1079
RsaI GTAC 2 cut(s) 743, 1181
RsaNI GTAC 2 cut(s) 742, 1180
SaqAI TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
SatI GCNGC 7 cut(s) 70, 719, 796, 940, 1077, 1131, 1260
Sau3AI GATC 8 cut(s) 34, 417, 517, 538, 766, 1140, 1371, 1479
Sau96I GGNCC 1 cut(s) 672
SchI GAGTC 2 cut(s) 623, 1035
ScrFI CCNGG 2 cut(s) 670, 1554
SduI GDGCHC 3 cut(s) 661, 732, 1489
SfaNI GCATC 4 cut(s) 34, 857, 1351, 1569
SfcI CTRYAG 2 cut(s) 688, 940
SinI GGWCC 1 cut(s) 672
SmlI CTYRAG 2 cut(s) 80, 1523
SmoI CTYRAG 2 cut(s) 80, 1523
Sse9I AATT 9 cut(s) 161, 191, 574, 734, 1095, 1186, 1204, 1391, 1442
SsiI CCGC 2 cut(s) 77, 1131
SspMI CTAG 2 cut(s) 681, 845
StyD4I CCNGG 2 cut(s) 668, 1552
StyI CCWWGG 4 cut(s) 675, 1045, 1263, 1452
TaaI ACNGT 5 cut(s) 15, 238, 341, 431, 1591
TaiI ACGT 1 cut(s) 743
TaqI TCGA 5 cut(s) 223, 233, 367, 837, 1345
TasI AATT 9 cut(s) 161, 191, 574, 734, 1095, 1186, 1204, 1391, 1442
TauI GCSGC 1 cut(s) 1133
TfiI GAWTC 3 cut(s) 439, 749, 822
Tru1I TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
Tru9I TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
TscAI CASTG 4 cut(s) 156, 346, 822, 1010
TseFI GTSAC 5 cut(s) 266, 615, 944, 1396, 1511
TseI GCWGC 6 cut(s) 69, 718, 795, 939, 1076, 1259
Tsp45I GTSAC 5 cut(s) 266, 615, 944, 1396, 1511
TspDTI ATGAA 7 cut(s) 461, 503, 568, 768, 927, 1288, 1485
TspGWI ACGGA 2 cut(s) 551, 1437
TspRI CASTG 4 cut(s) 156, 346, 822, 1010
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 2 cut(s) 363, 1023
XapI RAATTY 5 cut(s) 161, 574, 734, 1204, 1442
XceI RCATGY 2 cut(s) 61, 925
XmiI GTMKAC 1 cut(s) 1593
XmnI GAANNNNTTC 1 cut(s) 575
XspI CTAG 2 cut(s) 681, 845
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.