MD01G1145300.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011593
Physical Location & Seq
Reverse (-)
25534274 .. 25539376
5103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1145300.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGTCGAAATCCACTAGTGATCACAACTCATGCGCTAGGTCGAGGCGGCGGCATTTCGAGGCAACGGAGGGGCAACCGGGTTCCTCCACCAAGTTCGATGACGCCAATGCTCATGCCGTGTTTTTCCGCCGCAGGGTGCGAAAGTTGTCCGATTTCTCGAGCTGGCGGTTGTGCAAATGGACTTCAATGGTTTTGATGGGTTTGGCCTTTCTGTCAATGTTGGCCAAGTTTGCCTTGCTGAACACGTTTCCAGAGTTGAAGGTGATGGAGAAAATAAGTTTGATTCGACCTTCACTGATCGAAGGGACGACGACATTGAAACAGAACATGGTCAGATTAGGTGACAGGGTAAATGTGGCTAAAATGCTCATAAGTTCAAGGTCAGATTGTTATCTTTTACTGCATTTAATTTTAAAAAATTGCAGAAATTGTGATCCCATCTTTTATTTGATTTCTGCTAGTCAGACCCTTGATATTTGGACGCCATCAGAGAGTTATAACTACTACCAATGCATAAATCGAGCGAAGAAGGAATTTTGTACGTCCCGAAAGCTTTCTTCCCCAACAATGAATGAAACAGCCACAAATGGCTACATCCTTGTTCGTTCCAATGGCGGATTGAATCAAATGAAGCTCGGGGTATACACTAATAATCGCGCTAAGCTTTTCTGTTTTCGGATTTTCCAATTCGGTATATATTTCTTTTTCTTATTGCTTCTTATGAACAAACTGGAATTGCAGATAAGTGATATGGTTGCCATAGCAAAACTTATGAATGCCACTCTTGTTCTACCTTCTTTAGATCACATATCCTTTTGGACCGACCCAAGTGACTTTAAAGACATTTTCAATTGGAAGAACTTCATGGAAGTTTTGAGAGATGATATCATGATCGTAGAGTCTCTCCCGTCGGAGTTTGCAAACATGAAACCCCGCCATAAGGCTCCAGTATCTTGGTCAGGGGCCAGTTACTATAAACAACAGATGGCGACATTATTGAAGAAGCACAAGGTTGTCAAATTTACCTTCACAGATTCCAGACTTGCTAATAATGTTGATAGCTCGATCCAGAGACTCCGTTGCCGCGCCTTTTATAAGGCACTGCGCTTCGCAGTTGAAATCGAAGAGCTTGCAATGAATTTGATTTACAGATTAAAGAACAATGGTGGGAAATACATTGCTCTCCATCTAAGGTATGAAAAGGACATGCTTTCTTTCACAGGCTGCAGTCACAATCTCACTAGAGAAGAAGATTTAGAACTCGAAATGATAAGGGAGAAGACGCCACATTGGAAGCACAAAAAAATCAATGGTACAGTGCAGAGACACCAAGGGCAATGTCCCATGACCCCAAGAGAGATTGCTGTTTTCCTTGAGGCAATAGGGTTTCCTTCGGATACAAAAATTTACATAGCTGCAGGTGAAGTCTACGGGCAAGACGGGCTAACGTCACTGCAAGCCAAGTATCCAAATCTATTTTTCCACTCAAATTTAGCATCCGAAGAGGAGTTGCAACCTTACAAGGATAAGCTCAACCAGCTTGCCGCATTAGATTATATTATAGCAGTTGAGAGTGATGTTTTCATCTACTCCTATGATGGAAACATGGCCAAAGCAGTCAAAGGCCACAGAAGATTTGAAGGTTTTAGGAAAACAATCAGCCCCGACAAACAAAGATTTGTTAGATTGATCGATAAGTTAGACAAGGGTATGATAACTTGGAAAACATTCTCGTCCCGAGTTAAGAGGTTACACAAAAATCGGATGGGAGCACCATATCCAAGAGTGCCCAGAATTTTGCCTAGACTGGAGGAGAACTTTTATGCGAATCCATATCCAGGTTGCATTTGTGAAAGGACCAATGAGCCAGCTTCATCACCAGCAGGGAGATGTTCATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

635

Amino Acids

73.21

Weight (kDa)

9.58

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 242 - 546 9.1e-91 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 498, 1095
AarI CACCTGC 1 cut(s) 1409
Acc36I ACCTGC 1 cut(s) 1409
AccI GTMKAC 2 cut(s) 642, 1428
AccII CGCG 2 cut(s) 657, 1086
AciI CCGC 9 cut(s) 46, 49, 127, 130, 166, 615, 934, 1084, 1545
AclWI GGATC 2 cut(s) 428, 1060
AcoI YGGCCR 2 cut(s) 222, 1608
AcsI RAATTY 6 cut(s) 533, 1019, 1138, 1404, 1489, 1794
AcyI GRCGYC 3 cut(s) 102, 482, 1283
AfaI GTAC 2 cut(s) 541, 1315
AfiI CCNNNNNNNGG 3 cut(s) 133, 940, 1838
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 9 cut(s) 186, 259, 319, 378, 622, 850, 1000, 1118, 1640
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 1 cut(s) 1837
Alw21I GWGCWC 1 cut(s) 1774
Alw26I GTCTC 3 cut(s) 906, 1066, 1318
AlwI GGATC 2 cut(s) 428, 1060
Ama87I CYCGRG 3 cut(s) 157, 635, 1737
AoxI GGCC 5 cut(s) 204, 222, 963, 1608, 1624
ApeKI GCWGC 2 cut(s) 1224, 1415
ApoI RAATTY 6 cut(s) 533, 1019, 1138, 1404, 1489, 1794
Asp700I GAANNNNTTC 3 cut(s) 553, 860, 1727
AspLEI GCGC 4 cut(s) 35, 659, 1088, 1107
AspS9I GGNCC 3 cut(s) 819, 963, 1857
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 4 cut(s) 274, 353, 1433, 1869
AvaI CYCGRG 3 cut(s) 157, 635, 1737
AvaII GGWCC 2 cut(s) 819, 1857
BaeGI GKGCMC 1 cut(s) 1791
BalI TGGCCA 2 cut(s) 224, 1610
BbsI GAAGAC 1 cut(s) 1286
Bbv12I GWGCWC 1 cut(s) 1774
BbvI GCAGC 2 cut(s) 1211, 1402
BccI CCATC 8 cut(s) 190, 259, 446, 493, 979, 1194, 1592, 1759
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 1 cut(s) 1839
BciVI GTATCC 2 cut(s) 1390, 1476
BclI TGATCA 1 cut(s) 19
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 3 cut(s) 906, 1066, 1318
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 5 cut(s) 15, 36, 459, 1242, 1803
BfmI CTRYAG 2 cut(s) 1225, 1416
BfuAI ACCTGC 1 cut(s) 1409
BfuI GTATCC 2 cut(s) 1390, 1476
BisI GCNGC 7 cut(s) 47, 50, 130, 1084, 1225, 1416, 1545
BlpI GCTNAGC 1 cut(s) 660
BlsI GCNGC 7 cut(s) 48, 51, 131, 1085, 1226, 1417, 1546
Bme1390I CCNGG 2 cut(s) 78, 1839
Bme18I GGWCC 2 cut(s) 819, 1857
BmeT110I CYCGRG 3 cut(s) 157, 635, 1737
BmgT120I GGNCC 3 cut(s) 819, 963, 1857
BmiI GGNNCC 3 cut(s) 82, 945, 964
BmrFI CCNGG 2 cut(s) 78, 1839
BmsI GCATC 1 cut(s) 1505
BpiI GAAGAC 1 cut(s) 1286
BpmI CTGGAG 2 cut(s) 930, 1829
Bpu1102I GCTNAGC 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 1 cut(s) 78
Bsa29I ATCGAT 1 cut(s) 1692
BsaBI GATNNNNATC 2 cut(s) 390, 1894
BsaHI GRCGYC 3 cut(s) 102, 482, 1283
BsaJI CCNNGG 1 cut(s) 1330
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 940, 1838
Bse1I ACTGG 4 cut(s) 735, 947, 966, 1812
Bse3DI GCAATG 3 cut(s) 1140, 1176, 1343
Bse8I GATNNNNATC 2 cut(s) 390, 1894
BseBI CCWGG 1 cut(s) 1839
BseCI ATCGAT 1 cut(s) 1692
BseDI CCNNGG 1 cut(s) 1330
BseGI GGATG 4 cut(s) 594, 1496, 1770, 1895
BseJI GATNNNNATC 2 cut(s) 390, 1894
BseLI CCNNNNNNNGG 3 cut(s) 133, 940, 1838
BseMI GCAATG 3 cut(s) 1140, 1176, 1343
BseNI ACTGG 4 cut(s) 735, 947, 966, 1812
BseRI GAGGAG 2 cut(s) 1520, 1826
BseSI GKGCMC 1 cut(s) 1791
BseXI GCAGC 2 cut(s) 1211, 1402
BsgI GTGCAG 1 cut(s) 1340
Bsh1236I CGCG 2 cut(s) 657, 1086
BshFI GGCC 5 cut(s) 206, 224, 965, 1610, 1626
BshVI ATCGAT 1 cut(s) 1692
BsiHKAI GWGCWC 1 cut(s) 1774
BsiHKCI CYCGRG 3 cut(s) 157, 635, 1737
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 4 cut(s) 319, 529, 1326, 1720
BslI CCNNNNNNNGG 3 cut(s) 133, 940, 1838
BsmAI GTCTC 3 cut(s) 906, 1066, 1318
BsmFI GGGAC 4 cut(s) 319, 529, 1326, 1720
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 5 cut(s) 206, 224, 965, 1610, 1626
BsoBI CYCGRG 3 cut(s) 157, 635, 1737
Bsp1286I GDGCHC 2 cut(s) 1774, 1791
Bsp143I GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
Bsp1720I GCTNAGC 1 cut(s) 660
BspACI CCGC 9 cut(s) 46, 49, 127, 130, 166, 615, 934, 1084, 1545
BspANI GGCC 5 cut(s) 206, 224, 965, 1610, 1626
BspDI ATCGAT 1 cut(s) 1692
BspFNI CGCG 2 cut(s) 657, 1086
BspHI TCATGA 1 cut(s) 888
BspLI GGNNCC 3 cut(s) 82, 945, 964
BspMAI CTGCAG 2 cut(s) 1229, 1420
BspMI ACCTGC 1 cut(s) 1409
BspPI GGATC 2 cut(s) 428, 1060
BspQI GCTCTTC 1 cut(s) 1119
BsrDI GCAATG 3 cut(s) 1140, 1176, 1343
BsrI ACTGG 4 cut(s) 735, 947, 966, 1812
BssECI CCNNGG 1 cut(s) 1330
BssMI GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
BssNAI GTATAC 1 cut(s) 643
BssNI GRCGYC 3 cut(s) 102, 482, 1283
BssT1I CCWWGG 1 cut(s) 1330
Bst1107I GTATAC 1 cut(s) 643
Bst2UI CCWGG 1 cut(s) 1839
Bst4CI ACNGT 1 cut(s) 1318
Bst6I CTCTTC 2 cut(s) 1119, 1497
BstACI GRCGYC 3 cut(s) 102, 482, 1283
BstC8I GCNNGC 5 cut(s) 164, 1131, 1458, 1542, 1869
BstDEI CTNAG 2 cut(s) 660, 1190
BstF5I GGATG 4 cut(s) 594, 1496, 1770, 1895
BstFNI CGCG 2 cut(s) 657, 1086
BstHHI GCGC 4 cut(s) 35, 659, 1088, 1107
BstKTI GATC 7 cut(s) 22, 300, 436, 805, 894, 1068, 1692
BstMAI GTCTC 3 cut(s) 906, 1066, 1318
BstMBI GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
BstMWI GCNNNNNNNGC 3 cut(s) 230, 1441, 1537
BstNI CCWGG 1 cut(s) 1839
BstNSI RCATGY 1 cut(s) 1210
BstSCI CCNGG 2 cut(s) 76, 1837
BstSFI CTRYAG 2 cut(s) 1225, 1416
BstSLI GKGCMC 1 cut(s) 1791
BstUI CGCG 2 cut(s) 657, 1086
BstV1I GCAGC 2 cut(s) 1211, 1402
BstV2I GAAGAC 1 cut(s) 1286
BstXI CCANNNNNNTGG 1 cut(s) 954
BstZ17I GTATAC 1 cut(s) 643
Bsu15I ATCGAT 1 cut(s) 1692
BsuI GTATCC 2 cut(s) 1390, 1476
BsuRI GGCC 5 cut(s) 206, 224, 965, 1610, 1626
BsuTUI ATCGAT 1 cut(s) 1692
BtsCI GGATG 4 cut(s) 594, 1496, 1770, 1895
BtsI GCAGTG 2 cut(s) 1100, 1451
BtsIMutI CAGTG 4 cut(s) 293, 1100, 1323, 1451
BveI ACCTGC 1 cut(s) 1409
Cac8I GCNNGC 5 cut(s) 164, 1131, 1458, 1542, 1869
CciI TCATGA 1 cut(s) 888
CfoI GCGC 4 cut(s) 35, 659, 1088, 1107
Cfr13I GGNCC 3 cut(s) 819, 963, 1857
ClaI ATCGAT 1 cut(s) 1692
CseI GACGC 3 cut(s) 110, 490, 1291
Csp6I GTAC 2 cut(s) 540, 1314
CviAII CATG 9 cut(s) 30, 113, 328, 865, 889, 925, 1207, 1345, 1606
CviQI GTAC 2 cut(s) 540, 1314
DdeI CTNAG 2 cut(s) 660, 1190
DpnI GATC 7 cut(s) 21, 299, 435, 804, 893, 1067, 1691
DpnII GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
DraI TTTAAA 2 cut(s) 414, 838
EaeI YGGCCR 2 cut(s) 222, 1608
Eam1104I CTCTTC 2 cut(s) 1119, 1497
EarI CTCTTC 2 cut(s) 1119, 1497
EciI GGCGGA 2 cut(s) 116, 630
Eco130I CCWWGG 1 cut(s) 1330
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 2 cut(s) 819, 1857
Eco88I CYCGRG 3 cut(s) 157, 635, 1737
EcoRII CCWGG 1 cut(s) 1837
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 1330
EcoT22I ATGCAT 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 1330
FaeI CATG 9 cut(s) 33, 116, 331, 868, 892, 928, 1210, 1348, 1609
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 4 cut(s) 319, 529, 1326, 1720
FatI CATG 9 cut(s) 29, 112, 327, 864, 888, 924, 1206, 1344, 1605
FauI CCCGC 1 cut(s) 941
FbaI TGATCA 1 cut(s) 19
FblI GTMKAC 2 cut(s) 642, 1428
Fnu4HI GCNGC 7 cut(s) 47, 50, 130, 1084, 1225, 1416, 1545
FokI GGATG 4 cut(s) 581, 1483, 1777, 1882
Fsp4HI GCNGC 7 cut(s) 47, 50, 130, 1084, 1225, 1416, 1545
FspBI CTAG 5 cut(s) 15, 36, 459, 1242, 1803
GlaI GCGC 4 cut(s) 34, 658, 1087, 1106
GluI GCNGC 7 cut(s) 47, 50, 130, 1084, 1225, 1416, 1545
GsuI CTGGAG 2 cut(s) 930, 1829
HaeIII GGCC 5 cut(s) 206, 224, 965, 1610, 1626
HapII CCGG 1 cut(s) 77
HgaI GACGC 3 cut(s) 110, 490, 1291
HhaI GCGC 4 cut(s) 35, 659, 1088, 1107
Hin1I GRCGYC 3 cut(s) 102, 482, 1283
Hin1II CATG 9 cut(s) 33, 116, 331, 868, 892, 928, 1210, 1348, 1609
Hin6I GCGC 4 cut(s) 33, 657, 1086, 1105
HinP1I GCGC 4 cut(s) 33, 657, 1086, 1105
HindIII AAGCTT 2 cut(s) 551, 662
HinfI GANTC 6 cut(s) 283, 622, 899, 1034, 1074, 1828
HpaII CCGG 1 cut(s) 77
HphI GGTGA 4 cut(s) 274, 353, 1433, 1869
Hpy166II GTNNAC 2 cut(s) 643, 1429
Hpy188III TCNNGA 7 cut(s) 157, 251, 546, 889, 1038, 1069, 1737
Hpy8I GTNNAC 2 cut(s) 643, 1429
Hpy99I CGWCG 2 cut(s) 313, 913
HpyAV CCTTC 8 cut(s) 253, 296, 300, 523, 804, 1036, 1401, 1634
HpyCH4III ACNGT 1 cut(s) 1318
HpyCH4IV ACGT 3 cut(s) 245, 542, 1448
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 1441, 1537
HpyF3I CTNAG 2 cut(s) 660, 1190
HpySE526I ACGT 3 cut(s) 245, 542, 1448
Hsp92I GRCGYC 3 cut(s) 102, 482, 1283
Hsp92II CATG 9 cut(s) 33, 116, 331, 868, 892, 928, 1210, 1348, 1609
HspAI GCGC 4 cut(s) 33, 657, 1086, 1105
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
LguI GCTCTTC 1 cut(s) 1119
LmnI GCTCC 2 cut(s) 949, 1769
Lsp1109I GCAGC 2 cut(s) 1211, 1402
LweI GCATC 1 cut(s) 1505
MaeI CTAG 5 cut(s) 15, 36, 459, 1242, 1803
MaeII ACGT 3 cut(s) 245, 542, 1448
MaeIII GTNAC 6 cut(s) 341, 830, 968, 1229, 1449, 1749
MalI GATC 7 cut(s) 21, 299, 435, 804, 893, 1067, 1691
MboI GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
MfeI CAATTG 1 cut(s) 850
MhlI GDGCHC 2 cut(s) 1774, 1791
MlsI TGGCCA 2 cut(s) 224, 1610
MluNI TGGCCA 2 cut(s) 224, 1610
MlyI GAGTC 2 cut(s) 908, 1068
MmeI TCCRAC 1 cut(s) 891
MnlI CCTC 8 cut(s) 36, 52, 61, 94, 1369, 1498, 1740, 1804
Mox20I TGGCCA 2 cut(s) 224, 1610
Mph1103I ATGCAT 1 cut(s) 515
MroXI GAANNNNTTC 3 cut(s) 553, 860, 1727
MscI TGGCCA 2 cut(s) 224, 1610
MseI TTAA 5 cut(s) 407, 413, 837, 1154, 1743
MslI CAYNNNNRTG 1 cut(s) 1900
Msp20I TGGCCA 2 cut(s) 224, 1610
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 78, 1839
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 1 cut(s) 1839
MvnI CGCG 2 cut(s) 657, 1086
MwoI GCNNNNNNNGC 3 cut(s) 230, 1441, 1537
NciI CCSGG 1 cut(s) 78
NdeII GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
NlaIII CATG 9 cut(s) 33, 116, 331, 868, 892, 928, 1210, 1348, 1609
NlaIV GGNNCC 3 cut(s) 82, 945, 964
NmuCI GTSAC 4 cut(s) 341, 830, 1229, 1449
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 1 cut(s) 1210
PaeR7I CTCGAG 1 cut(s) 157
PagI TCATGA 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 1409
PciSI GCTCTTC 1 cut(s) 1119
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 3 cut(s) 553, 860, 1727
PfeI GAWTC 4 cut(s) 283, 622, 1034, 1828
PkrI GCNGC 7 cut(s) 48, 51, 131, 1085, 1226, 1417, 1546
PleI GAGTC 2 cut(s) 907, 1068
PpsI GAGTC 2 cut(s) 907, 1068
PsiI TTATAA 2 cut(s) 498, 1095
Psp6I CCWGG 1 cut(s) 1837
PspGI CCWGG 1 cut(s) 1837
PspN4I GGNNCC 3 cut(s) 82, 945, 964
PspPI GGNCC 3 cut(s) 819, 963, 1857
PstI CTGCAG 2 cut(s) 1229, 1420
RsaI GTAC 2 cut(s) 541, 1315
RsaNI GTAC 2 cut(s) 540, 1314
RseI CAYNNNNRTG 1 cut(s) 1900
SapI GCTCTTC 1 cut(s) 1119
SaqAI TTAA 5 cut(s) 407, 413, 837, 1154, 1743
SatI GCNGC 7 cut(s) 47, 50, 130, 1084, 1225, 1416, 1545
Sau3AI GATC 7 cut(s) 19, 297, 433, 802, 891, 1065, 1689
Sau96I GGNCC 3 cut(s) 819, 963, 1857
SchI GAGTC 2 cut(s) 908, 1068
ScrFI CCNGG 2 cut(s) 78, 1839
SduI GDGCHC 2 cut(s) 1774, 1791
SfaNI GCATC 1 cut(s) 1505
SfcI CTRYAG 2 cut(s) 1225, 1416
Sfr274I CTCGAG 1 cut(s) 157
SinI GGWCC 2 cut(s) 819, 1857
SlaI CTCGAG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 1900
SmlI CTYRAG 2 cut(s) 157, 1373
SmoI CTYRAG 2 cut(s) 157, 1373
SpeI ACTAGT 1 cut(s) 14
SsiI CCGC 9 cut(s) 46, 49, 127, 130, 166, 615, 934, 1084, 1545
SspMI CTAG 5 cut(s) 15, 36, 459, 1242, 1803
StyD4I CCNGG 2 cut(s) 76, 1837
StyI CCWWGG 1 cut(s) 1330
TaaI ACNGT 1 cut(s) 1318
TaiI ACGT 3 cut(s) 248, 545, 1451
TaqII GACCGA 1 cut(s) 836
TauI GCSGC 5 cut(s) 49, 52, 132, 1086, 1547
TfiI GAWTC 4 cut(s) 283, 622, 1034, 1828
Tru1I TTAA 5 cut(s) 407, 413, 837, 1154, 1743
Tru9I TTAA 5 cut(s) 407, 413, 837, 1154, 1743
TscAI CASTG 4 cut(s) 300, 1107, 1323, 1458
TseFI GTSAC 4 cut(s) 341, 830, 1229, 1449
TseI GCWGC 2 cut(s) 1224, 1415
Tsp45I GTSAC 4 cut(s) 341, 830, 1229, 1449
TspGWI ACGGA 2 cut(s) 80, 1067
TspRI CASTG 4 cut(s) 300, 1107, 1323, 1458
VpaK11BI GGWCC 2 cut(s) 819, 1857
XapI RAATTY 6 cut(s) 533, 1019, 1138, 1404, 1489, 1794
XceI RCATGY 1 cut(s) 1210
XhoI CTCGAG 1 cut(s) 157
XmiI GTMKAC 2 cut(s) 642, 1428
XmnI GAANNNNTTC 3 cut(s) 553, 860, 1727
XspI CTAG 5 cut(s) 15, 36, 459, 1242, 1803
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.