MD07G1212400.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011593
Physical Location & Seq
Reverse (-)
29051792 .. 29052568
777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1212400.v1.1.491

Sequence Viewer

Length: 546 bp
ATGACCCCAAGAGAGGTTACTCTTTTCCTTGAGGCAATAGGGTTTCCTTCGGATACGAAAATTTACATAGTTTCCGGTGAGATGTATGGGCAAGACGGGCTAACATCATTGCAAGCTAAGTATCCGAATCTATTTTTCCACTCAAATTTAGCATATGAAGATGAGTTACAACCTTACAAGGACAAGCTCAATCAGCTTGTTGCTTTGGACTATATTATAGCAGTTGAGAGTGATGGGTATGTAACATGTTGTATTGGGAGTTTTGTATCTCACACATTGGATGTTACTTTGCCTTGCAAACAAAGAATTGTGAGATTGATTGACAAGTTGGACGAGGGGGCGATATCTTGGAAAACATTCTCGTTAAGGGTTAAGAGGTTACACGAAAATCGGATCGGAGCACCGTACCCCAGAGTGCCAAGAAGTTTGCCTAGACTGGAGGAGAACTTTTATGCAAATCCATATCCAGGTTGCATTTGTGAAAAGCCCAAAGAGCCAGCTTCATCACTAGCAAGGAGAAATATGTTAAAACGATTAGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

182

Amino Acids

20.65

Weight (kDa)

8.23

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 89 4.4e-17 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 2 cut(s) 60, 145
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 2 cut(s) 13, 467
AflIII ACRYGT 1 cut(s) 245
AjnI CCWGG 1 cut(s) 466
AluBI AGCT 4 cut(s) 116, 187, 196, 500
AluI AGCT 4 cut(s) 116, 187, 196, 500
Alw21I GWGCWC 1 cut(s) 403
AlwI GGATC 1 cut(s) 401
ApoI RAATTY 2 cut(s) 60, 145
Asp700I GAANNNNTTC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
Bbv12I GWGCWC 1 cut(s) 403
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 468
BciVI GTATCC 2 cut(s) 46, 132
BfaI CTAG 2 cut(s) 432, 509
BfuI GTATCC 2 cut(s) 46, 132
Bme1390I CCNGG 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 467
Bse1I ACTGG 1 cut(s) 441
Bse3DI GCAATG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 468
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 13, 467
BseMI GCAATG 1 cut(s) 107
BseNI ACTGG 1 cut(s) 441
BseRI GAGGAG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 13, 467
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 1 cut(s) 393
BspPI GGATC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 107
BsrI ACTGG 1 cut(s) 441
BssMI GATC 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 114, 498
BstDEI CTNAG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 1 cut(s) 396
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 3 cut(s) 97, 193, 493
BstNI CCWGG 1 cut(s) 468
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 466
BsuI GTATCC 2 cut(s) 46, 132
BtsCI GGATG 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 114, 498
Csp6I GTAC 1 cut(s) 406
CviAII CATG 1 cut(s) 246
CviJI RGCY 7 cut(s) 100, 116, 187, 196, 487, 496, 500
CviKI_1 RGCY 7 cut(s) 100, 116, 187, 196, 487, 496, 500
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 1 cut(s) 117
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 345
FaeI CATG 1 cut(s) 249
FatI CATG 1 cut(s) 245
FauNDI CATATG 1 cut(s) 154
FokI GGATG 1 cut(s) 293
FspBI CTAG 2 cut(s) 432, 509
GsuI CTGGAG 1 cut(s) 458
HapII CCGG 1 cut(s) 75
Hin1II CATG 1 cut(s) 249
HinfI GANTC 1 cut(s) 127
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 4 cut(s) 52, 126, 393, 398
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4V TGCA 4 cut(s) 112, 297, 455, 474
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 193, 493
HpyF3I CTNAG 1 cut(s) 117
Hsp92II CATG 1 cut(s) 249
Kzo9I GATC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 6 cut(s) 88, 422, 424, 453, 480, 510
MaeI CTAG 2 cut(s) 432, 509
MaeIII GTNAC 5 cut(s) 16, 165, 241, 283, 378
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MboII GAAGA 1 cut(s) 170
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 3 cut(s) 60, 145, 306
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 5 cut(s) 7, 25, 328, 369, 433
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 3 cut(s) 365, 372, 527
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 3 cut(s) 97, 193, 493
NdeI CATATG 1 cut(s) 154
NdeII GATC 1 cut(s) 393
NlaIII CATG 1 cut(s) 249
NspI RCATGY 1 cut(s) 249
PciI ACATGT 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 1 cut(s) 127
PscI ACATGT 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SaqAI TTAA 3 cut(s) 365, 372, 527
Sau3AI GATC 1 cut(s) 393
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 1 cut(s) 403
SetI ASST 8 cut(s) 18, 118, 175, 189, 198, 380, 472, 502
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 60, 145, 306
SspMI CTAG 2 cut(s) 432, 509
StyD4I CCNGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 405
TasI AATT 3 cut(s) 60, 145, 306
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 3 cut(s) 365, 372, 527
Tru9I TTAA 3 cut(s) 365, 372, 527
TspDTI ATGAA 2 cut(s) 171, 492
XapI RAATTY 2 cut(s) 60, 145
XceI RCATGY 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 2 cut(s) 432, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.