Rh1AG355600

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0011593
Physical Location & Seq
Forward (+)
59138744 .. 59141554
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG355600.1

Sequence Viewer

Length: 1194 bp
ATGATCAAGGCCAATGGTGGGTTGAATCAAATGAAGCTTGGGATAAGTGATATGGTTGCCGTAGCAAAACTTATGAATGCCACTCTGGTTCTCCCTTTTTTAGATCATAAATCCTTTTGGACCGATCCAAGTGATTTTAAGGACATTTTCAACTGGAAGAATTTCATGGAAGTCTTAAAAGATGATATCATGGTTATAGAGTCACTCCCACCGGAGCTTGCAGACCTGAAGCCTCTCCCCAGGGCTCCAGTTTCCTGGTCCAAGGCTAGTTACTACAGAGGATATATGTCTTCGTTATTGAAGCAGCACAAAGTGCTCAAATTCACGTACACAGATTCCAGACTTGCTAATGATCTTGGTGGTTCATTCCAAAAGCTACGCTGCCGTGCAATGTTTGAGGCACTGAGATTCTCACCTGAAATCGAACAACTAGCATCAAAGTTGGTTAACAGATTAAAGAACAACAATAGTAGATACATTGGTCTCCATTTAAGATATGAAAAGGACATGCTTTCTTTCACAGGCTGCAGTCACAATCTGACCAAAGACGAAGATAGAGAACTCGCAAAAATAAGGAAACAAACATCACACTGGAAGCATAAGACGATTAATAGCACCAATCATAGACTCCAAGGGGTATGCCCCATGACCCCAAGAGAGGTTGCTGTGTTTCTTGAAGCAATTGGGTTTCCTTTGGATACAAAAGTTTATGTAGCCGCTGGGAAGATCTACGGGAGAGATGGGATTAGACCTCTGAAAGATAAGTACCCCAATTTATTCTTCCACTCAAATTTAGCGACTGAAGTAGAGTTGCAACCTTTCAAGAACTTGCAGAACCAGCTTGCTGCCTTGGATTACATTTTGGCTTTGGAGAGTGATGTGTTCATTTATTCTTTTGATGGAAATATGGCTAAAGCAATAAAAGGGCATCGACGATTTGAAGGGTTCCGGAAGACGATCAACCCCAACAAACAACAATTTGTGACATTGATTGATAGGTTGGACAACGGAATGATATCTTGGGAAAAATTCTCATCCAAGGTGAAGAAGTTGCATGAAAATCGGATCGGAGCACCATACTCCAGAGTAGCCAGAGGTGACCACAAACTTGAGGAGAACTTTTACGCAAACCCTTTTCCGGGTTGCATCTGTGAAAATTCCGGAGGCTCATCAACAGTAGCCAAGCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

397

Amino Acids

45.44

Weight (kDa)

9.47

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 2 - 313 5.3e-95 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 948, 1160
AciI CCGC 1 cut(s) 717
AclWI GGATC 2 cut(s) 119, 1073
AcsI RAATTY 5 cut(s) 160, 320, 790, 1028, 1156
AcuI CTGAAG 2 cut(s) 248, 822
AdeI CACNNNGTG 1 cut(s) 313
AfaI GTAC 2 cut(s) 329, 767
AfiI CCNNNNNNNGG 4 cut(s) 18, 658, 1138, 1139
AgsI TTSAA 6 cut(s) 25, 151, 301, 677, 823, 941
AjnI CCWGG 2 cut(s) 239, 254
AjuI GAANNNNNNNTTGG 4 cut(s) 764, 796, 1003, 1035
AluBI AGCT 4 cut(s) 37, 217, 376, 841
AluI AGCT 4 cut(s) 37, 217, 376, 841
Alw21I GWGCWC 2 cut(s) 318, 1075
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 2 cut(s) 119, 1073
Aor13HI TCCGGA 2 cut(s) 948, 1160
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 4 cut(s) 304, 381, 525, 845
ApoI RAATTY 5 cut(s) 160, 320, 790, 1028, 1156
AseI ATTAAT 1 cut(s) 609
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 120, 258
AsuC2I CCSGG 1 cut(s) 1140
AsuHPI GGTGA 3 cut(s) 405, 1054, 1109
AvaII GGWCC 2 cut(s) 120, 258
BaeI ACNNNNGTAYC 4 cut(s) 466, 499, 690, 723
BanII GRGCYC 1 cut(s) 247
BbsI GAAGAC 2 cut(s) 282, 959
Bbv12I GWGCWC 2 cut(s) 318, 1075
BbvI GCAGC 4 cut(s) 316, 368, 512, 832
BccI CCATC 2 cut(s) 734, 893
BceAI ACGGC 2 cut(s) 44, 369
BcgI CGANNNNNNTGC 2 cut(s) 1043, 1077
BciT130I CCWGG 2 cut(s) 241, 256
BciVI GTATCC 1 cut(s) 691
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1140
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 2 cut(s) 267, 431
BfmI CTRYAG 2 cut(s) 274, 526
BfuI GTATCC 1 cut(s) 691
BglII AGATCT 1 cut(s) 726
BisI GCNGC 5 cut(s) 305, 382, 526, 717, 846
BlsI GCNGC 5 cut(s) 306, 383, 527, 718, 847
Bme1390I CCNGG 3 cut(s) 241, 256, 1140
Bme18I GGWCC 2 cut(s) 120, 258
BmgT120I GGNCC 2 cut(s) 120, 258
BmiI GGNNCC 2 cut(s) 246, 947
BmrFI CCNGG 3 cut(s) 241, 256, 1140
BmsI GCATC 3 cut(s) 443, 937, 1155
BpiI GAAGAC 2 cut(s) 282, 959
BpmI CTGGAG 2 cut(s) 231, 1066
BpuEI CTTGAG 1 cut(s) 1130
BpuMI CCSGG 1 cut(s) 1140
BsaAI YACGTR 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 6 cut(s) 239, 240, 261, 631, 849, 1038
BsaWI WCCGGW 3 cut(s) 211, 948, 1160
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 658, 1138, 1139
Bse1I ACTGG 3 cut(s) 158, 248, 596
Bse3DI GCAATG 1 cut(s) 396
BseAI TCCGGA 2 cut(s) 948, 1160
BseBI CCWGG 2 cut(s) 241, 256
BseDI CCNNGG 6 cut(s) 239, 240, 261, 631, 849, 1038
BseGI GGATG 1 cut(s) 1034
BseLI CCNNNNNNNGG 4 cut(s) 18, 658, 1138, 1139
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 3 cut(s) 158, 248, 596
BseRI GAGGAG 1 cut(s) 1127
BseXI GCAGC 4 cut(s) 316, 368, 512, 832
BseYI CCCAGC 1 cut(s) 719
BshFI GGCC 1 cut(s) 11
BsiHKAI GWGCWC 2 cut(s) 318, 1075
BsiSI CCGG 4 cut(s) 212, 949, 1139, 1161
BslI CCNNNNNNNGG 4 cut(s) 18, 658, 1138, 1139
BsmAI GTCTC 1 cut(s) 488
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 247, 318, 1075
Bsp13I TCCGGA 2 cut(s) 948, 1160
Bsp143I GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
BspACI CCGC 1 cut(s) 717
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 396
BspEI TCCGGA 2 cut(s) 948, 1160
BspLI GGNNCC 2 cut(s) 246, 947
BspMAI CTGCAG 1 cut(s) 530
BspPI GGATC 2 cut(s) 119, 1073
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 3 cut(s) 158, 248, 596
BssECI CCNNGG 6 cut(s) 239, 240, 261, 631, 849, 1038
BssMI GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
BssT1I CCWWGG 4 cut(s) 261, 631, 849, 1038
Bst2UI CCWGG 2 cut(s) 241, 256
Bst4CI ACNGT 1 cut(s) 1177
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 219, 843
BstDEI CTNAG 1 cut(s) 404
BstEII GGTNACC 1 cut(s) 1097
BstF5I GGATG 1 cut(s) 1034
BstKTI GATC 7 cut(s) 6, 106, 127, 355, 729, 960, 1068
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
BstMWI GCNNNNNNNGC 2 cut(s) 313, 838
BstNI CCWGG 2 cut(s) 241, 256
BstNSI RCATGY 1 cut(s) 511
BstPI GGTNACC 1 cut(s) 1097
BstSCI CCNGG 3 cut(s) 239, 254, 1138
BstSFI CTRYAG 2 cut(s) 274, 526
BstV1I GCAGC 4 cut(s) 316, 368, 512, 832
BstV2I GAAGAC 2 cut(s) 282, 959
BstX2I RGATCY 1 cut(s) 726
BstXI CCANNNNNNTGG 1 cut(s) 255
BstYI RGATCY 1 cut(s) 726
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 1034
BtsIMutI CAGTG 2 cut(s) 401, 589
Cac8I GCNNGC 2 cut(s) 219, 843
Cfr13I GGNCC 2 cut(s) 120, 258
Csp6I GTAC 2 cut(s) 328, 766
CviAII CATG 5 cut(s) 166, 190, 508, 646, 1055
CviQI GTAC 2 cut(s) 328, 766
DdeI CTNAG 1 cut(s) 404
DpnI GATC 7 cut(s) 5, 105, 126, 354, 728, 959, 1067
DpnII GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
DraIII CACNNNGTG 1 cut(s) 313
Eco130I CCWWGG 4 cut(s) 261, 631, 849, 1038
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 488
Eco32I GATATC 2 cut(s) 187, 1017
Eco47I GGWCC 2 cut(s) 120, 258
Eco57I CTGAAG 2 cut(s) 248, 822
Eco91I GGTNACC 1 cut(s) 1097
EcoO65I GGTNACC 1 cut(s) 1097
EcoRII CCWGG 2 cut(s) 239, 254
EcoRV GATATC 2 cut(s) 187, 1017
EcoT14I CCWWGG 4 cut(s) 261, 631, 849, 1038
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 4 cut(s) 261, 631, 849, 1038
FaeI CATG 5 cut(s) 169, 193, 511, 649, 1058
FatI CATG 5 cut(s) 165, 189, 507, 645, 1054
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 5 cut(s) 305, 382, 526, 717, 846
FokI GGATG 1 cut(s) 1021
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 5 cut(s) 305, 382, 526, 717, 846
FspBI CTAG 2 cut(s) 267, 431
GluI GCNGC 5 cut(s) 305, 382, 526, 717, 846
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 2 cut(s) 231, 1066
HaeIII GGCC 1 cut(s) 11
HapII CCGG 4 cut(s) 212, 949, 1139, 1161
Hin1II CATG 5 cut(s) 169, 193, 511, 649, 1058
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 5 cut(s) 25, 200, 335, 408, 627
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 4 cut(s) 212, 949, 1139, 1161
HphI GGTGA 3 cut(s) 405, 1054, 1109
Hpy166II GTNNAC 2 cut(s) 330, 448
Hpy188I TCNGA 4 cut(s) 540, 756, 1065, 1070
Hpy188III TCNNGA 6 cut(s) 339, 674, 823, 949, 1083, 1161
Hpy8I GTNNAC 2 cut(s) 330, 448
Hpy99I CGWCG 1 cut(s) 936
HpyAV CCTTC 1 cut(s) 935
HpyCH4III ACNGT 1 cut(s) 1177
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 7 cut(s) 221, 389, 528, 814, 832, 1054, 1146
HpyF10VI GCNNNNNNNGC 2 cut(s) 313, 838
HpyF3I CTNAG 1 cut(s) 404
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 5 cut(s) 169, 193, 511, 649, 1058
Kpn2I TCCGGA 2 cut(s) 948, 1160
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
LmnI GCTCC 3 cut(s) 214, 250, 1070
Lsp1109I GCAGC 4 cut(s) 316, 368, 512, 832
LweI GCATC 3 cut(s) 443, 937, 1155
MaeI CTAG 2 cut(s) 267, 431
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 5 cut(s) 201, 269, 530, 982, 1097
MalI GATC 7 cut(s) 5, 105, 126, 354, 728, 959, 1067
MboI GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
MboII GAAGA 7 cut(s) 169, 282, 563, 736, 772, 964, 1057
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 1 cut(s) 726
MhlI GDGCHC 3 cut(s) 247, 318, 1075
MluCI AATT 8 cut(s) 160, 320, 681, 772, 790, 977, 1028, 1156
MlyI GAGTC 2 cut(s) 209, 621
MmeI TCCRAC 1 cut(s) 981
MnlI CCTC 8 cut(s) 243, 272, 391, 652, 762, 1088, 1105, 1157
MroI TCCGGA 2 cut(s) 948, 1160
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 6 cut(s) 138, 176, 447, 455, 491, 609
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 4 cut(s) 212, 949, 1139, 1161
MspR9I CCNGG 3 cut(s) 241, 256, 1140
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 2 cut(s) 241, 256
MwoI GCNNNNNNNGC 2 cut(s) 313, 838
NciI CCSGG 1 cut(s) 1140
NdeII GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
NlaIII CATG 5 cut(s) 169, 193, 511, 649, 1058
NlaIV GGNNCC 2 cut(s) 246, 947
NmuCI GTSAC 4 cut(s) 201, 530, 982, 1097
NspI RCATGY 1 cut(s) 511
PasI CCCWGGG 1 cut(s) 240
PctI GAATGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 3 cut(s) 25, 335, 408
PkrI GCNGC 5 cut(s) 306, 383, 527, 718, 847
PleI GAGTC 2 cut(s) 208, 621
PpsI GAGTC 2 cut(s) 208, 621
Ppu21I YACGTR 1 cut(s) 327
PshBI ATTAAT 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 239, 254
PspEI GGTNACC 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 2 cut(s) 239, 254
PspN4I GGNNCC 2 cut(s) 246, 947
PspPI GGNCC 2 cut(s) 120, 258
PstI CTGCAG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 726
RsaI GTAC 2 cut(s) 329, 767
RsaNI GTAC 2 cut(s) 328, 766
SaqAI TTAA 6 cut(s) 138, 176, 447, 455, 491, 609
SatI GCNGC 5 cut(s) 305, 382, 526, 717, 846
Sau3AI GATC 7 cut(s) 3, 103, 124, 352, 726, 957, 1065
Sau96I GGNCC 2 cut(s) 120, 258
SchI GAGTC 2 cut(s) 209, 621
ScrFI CCNGG 3 cut(s) 241, 256, 1140
SduI GDGCHC 3 cut(s) 247, 318, 1075
SfaNI GCATC 3 cut(s) 443, 937, 1155
SfcI CTRYAG 2 cut(s) 274, 526
SinI GGWCC 2 cut(s) 120, 258
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
Sse9I AATT 8 cut(s) 160, 320, 681, 772, 790, 977, 1028, 1156
SsiI CCGC 1 cut(s) 717
SspMI CTAG 2 cut(s) 267, 431
StyD4I CCNGG 3 cut(s) 239, 254, 1138
StyI CCWWGG 4 cut(s) 261, 631, 849, 1038
TaaI ACNGT 1 cut(s) 1177
TaiI ACGT 1 cut(s) 329
TaqI TCGA 2 cut(s) 423, 931
TaqII GACCGA 1 cut(s) 137
TasI AATT 8 cut(s) 160, 320, 681, 772, 790, 977, 1028, 1156
TauI GCSGC 1 cut(s) 719
TfiI GAWTC 3 cut(s) 25, 335, 408
Tru1I TTAA 6 cut(s) 138, 176, 447, 455, 491, 609
Tru9I TTAA 6 cut(s) 138, 176, 447, 455, 491, 609
TscAI CASTG 2 cut(s) 408, 596
TseFI GTSAC 4 cut(s) 201, 530, 982, 1097
TseI GCWGC 4 cut(s) 304, 381, 525, 845
Tsp45I GTSAC 4 cut(s) 201, 530, 982, 1097
TspDTI ATGAA 7 cut(s) 47, 89, 154, 354, 513, 874, 1071
TspGWI ACGGA 1 cut(s) 1023
TspRI CASTG 2 cut(s) 408, 596
VpaK11BI GGWCC 2 cut(s) 120, 258
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 5 cut(s) 160, 320, 790, 1028, 1156
XceI RCATGY 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 2 cut(s) 267, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.