Rw1G031380

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0011593
Physical Location & Seq
Forward (+)
59536586 .. 59539226
2641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031380.1

Sequence Viewer

Length: 1392 bp
ATGACAAGGAGACGGTTATTTGAGGCCACAGAAGATCAACAAGATGCCATTAACAGCATGTTCTTCAGCAGCAGACCGCTCAAGTTGGCAAAGCTTTTGATGAGAGGAAGAAGGGTTTTCGTGGTTTTGTTGCTGTTAGGTTTCGTGTCAGTGATGACAAAATTTGCTTTACAGCTGAATGCATTACAGGAATTGAGAATGAGGGAAGGAATAGCTTTTATTCGACGACCTTCGACAGTTTTGCAACATAAAATGGTTAGTGAAGGTGACGAGGGTTATAATGTGGCTCTGAAACAACGAAGTTCCCGTCAGACTGGTGATATCTGGACGCAACCAAACAGTGAAAACTACTACAAATGCATTAATCGAGCAAAGAAGGATATTTTGAAACGAAATGCAACAAATGGCTATATTATGATCAAGGCCAATGGTGGGTTGAATCAAATGAAGCTTGGGATAAGTGATATGGTTGCCGTAGCAAAACTTATGAATGCCACTCTGGTTCTCCCTTTTTTAGATCATAAATCCTTTTGGACCGATCCAAGTGATTTTAAGGACATTTTCAACTGGAAGAATTTCATGGAAGTCTTAAAAGATGATATCATGGTTATAGAGTCACTCCCACCGGAGCTTGCAGACCTGAAGCCTCTCCCCAGGGCTCCAGTTTCCTGGTCCAAGGCTAGTTACTACAGAGGATATATGTCTTCGTTATTGAAGCAGCACAAAGTGCTCAAATTCACGTACACAGATTCCAGACTTGCTAATGATCTTGGTGGTTCATTCCAAAAGCTACGCTGCCGTGCAATGTTTGAGGCACTGAGATTCTCACCTGAAATCGAACAACTAGCATCAAAGTTGGTTAACAGATTAAAGAACAACAATAGTAGATACATTGGTCTCCATTTAAGATATGAAAAGGACATGCTTTCTTTCACAGGCTGCAGTCACAATCTGACCAAAGACGAAGATAGAGAACTCGCAAAAATAAGGAAACAAACATCACACTGGAAGCATAAGACGATTAATAGCACCAATCATAGACTCCAAGGGGTATGCCCCATGACCCCAAGAGAGGTTGCTGTGTTTCTTGAAGCAATTGGGTTTCCTTTGGATACAAAAGTTTATGTAGCCGCTGGGAAGATCTACGGGAGAGATGGGATTAGACCTCTGAAAGATAAGTACCCCAATTTATTCTTCCACTCAAATTTAGCGACTGAAGTAGAGTTGCAACCTTTCAAGAACTTGCAGAACCAGCTTGCTGCCTTGGATTACATTTTGGCTTTGGAGAGTGATGTGTTCATTTATTCTTTTGATGGAAATATGGCTAAAGCAATAAAAGGGCATCGACGATTTGAAGGGTTCCGGAAGACGATCAACCCCAACAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

463

Amino Acids

53.41

Weight (kDa)

9.78

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 137 - 451 1.9e-96 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 79
AccIII TCCGGA 1 cut(s) 1362
AciI CCGC 2 cut(s) 77, 1131
AclWI GGATC 1 cut(s) 533
AcsI RAATTY 4 cut(s) 161, 574, 734, 1204
AcuI CTGAAG 3 cut(s) 49, 662, 1236
AdeI CACNNNGTG 1 cut(s) 727
AfaI GTAC 2 cut(s) 743, 1181
AfiI CCNNNNNNNGG 2 cut(s) 432, 1072
AgsI TTSAA 7 cut(s) 388, 439, 565, 715, 1091, 1237, 1355
AjnI CCWGG 2 cut(s) 653, 668
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
AluBI AGCT 7 cut(s) 94, 175, 215, 451, 631, 790, 1255
AluI AGCT 7 cut(s) 94, 175, 215, 451, 631, 790, 1255
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 2 cut(s) 4, 902
AlwI GGATC 1 cut(s) 533
Aor13HI TCCGGA 1 cut(s) 1362
AoxI GGCC 2 cut(s) 24, 423
ApeKI GCWGC 5 cut(s) 69, 718, 795, 939, 1259
ApoI RAATTY 4 cut(s) 161, 574, 734, 1204
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
AseI ATTAAT 2 cut(s) 363, 1023
Asp700I GAANNNNTTC 1 cut(s) 575
AspS9I GGNCC 2 cut(s) 534, 672
AsuHPI GGTGA 3 cut(s) 278, 329, 819
AvaII GGWCC 2 cut(s) 534, 672
BaeI ACNNNNGTAYC 4 cut(s) 880, 913, 1104, 1137
BanII GRGCYC 1 cut(s) 661
BbsI GAAGAC 2 cut(s) 696, 1373
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 5 cut(s) 81, 730, 782, 926, 1246
BccI CCATC 2 cut(s) 1148, 1307
BceAI ACGGC 2 cut(s) 458, 783
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 2 cut(s) 655, 670
BciVI GTATCC 1 cut(s) 1105
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 2 cut(s) 4, 902
BfaI CTAG 2 cut(s) 681, 845
BfmI CTRYAG 2 cut(s) 688, 940
BfuI GTATCC 1 cut(s) 1105
BglII AGATCT 1 cut(s) 1140
BisI GCNGC 6 cut(s) 70, 719, 796, 940, 1131, 1260
BlsI GCNGC 6 cut(s) 71, 720, 797, 941, 1132, 1261
Bme1390I CCNGG 2 cut(s) 655, 670
Bme18I GGWCC 2 cut(s) 534, 672
BmgT120I GGNCC 2 cut(s) 534, 672
BmiI GGNNCC 2 cut(s) 660, 1361
BmrFI CCNGG 2 cut(s) 655, 670
BmsI GCATC 3 cut(s) 34, 857, 1351
BpiI GAAGAC 2 cut(s) 696, 1373
BpmI CTGGAG 1 cut(s) 645
BpuEI CTTGAG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 741
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 5 cut(s) 653, 654, 675, 1045, 1263
BsaWI WCCGGW 2 cut(s) 625, 1362
Bsc4I CCNNNNNNNGG 2 cut(s) 432, 1072
Bse1I ACTGG 4 cut(s) 319, 572, 662, 1010
Bse3DI GCAATG 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 1362
BseBI CCWGG 2 cut(s) 655, 670
BseDI CCNNGG 5 cut(s) 653, 654, 675, 1045, 1263
BseLI CCNNNNNNNGG 2 cut(s) 432, 1072
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 1 cut(s) 809
BseNI ACTGG 4 cut(s) 319, 572, 662, 1010
BseXI GCAGC 5 cut(s) 81, 730, 782, 926, 1246
BseYI CCCAGC 1 cut(s) 1133
BshFI GGCC 2 cut(s) 26, 425
BsiHKAI GWGCWC 1 cut(s) 732
BsiSI CCGG 2 cut(s) 626, 1363
BslI CCNNNNNNNGG 2 cut(s) 432, 1072
BsmAI GTCTC 2 cut(s) 4, 902
BsmBI CGTCTC 1 cut(s) 4
BsmI GAATGC 2 cut(s) 184, 496
BsnI GGCC 2 cut(s) 26, 425
Bso31I GGTCTC 1 cut(s) 902
Bsp1286I GDGCHC 2 cut(s) 661, 732
Bsp13I TCCGGA 1 cut(s) 1362
Bsp143I GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
BspACI CCGC 2 cut(s) 77, 1131
BspANI GGCC 2 cut(s) 26, 425
BspCNI CTCAG 1 cut(s) 810
BspEI TCCGGA 1 cut(s) 1362
BspLI GGNNCC 2 cut(s) 660, 1361
BspMAI CTGCAG 1 cut(s) 944
BspPI GGATC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 4 cut(s) 319, 572, 662, 1010
BssECI CCNNGG 5 cut(s) 653, 654, 675, 1045, 1263
BssMI GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
BssT1I CCWWGG 3 cut(s) 675, 1045, 1263
Bst2UI CCWGG 2 cut(s) 655, 670
Bst4CI ACNGT 3 cut(s) 15, 238, 341
BstAPI GCANNNNNTGC 1 cut(s) 727
BstBAI YACGTR 1 cut(s) 741
BstC8I GCNNGC 2 cut(s) 633, 1257
BstDEI CTNAG 1 cut(s) 818
BstKTI GATC 7 cut(s) 37, 420, 520, 541, 769, 1143, 1374
BstMAI GTCTC 2 cut(s) 4, 902
BstMBI GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
BstMWI GCNNNNNNNGC 2 cut(s) 727, 1252
BstNI CCWGG 2 cut(s) 655, 670
BstNSI RCATGY 2 cut(s) 61, 925
BstSCI CCNGG 2 cut(s) 653, 668
BstSFI CTRYAG 2 cut(s) 688, 940
BstV1I GCAGC 5 cut(s) 81, 730, 782, 926, 1246
BstV2I GAAGAC 2 cut(s) 696, 1373
BstX2I RGATCY 1 cut(s) 1140
BstXI CCANNNNNNTGG 1 cut(s) 669
BstYI RGATCY 1 cut(s) 1140
BsuI GTATCC 1 cut(s) 1105
BsuRI GGCC 2 cut(s) 26, 425
BtsIMutI CAGTG 4 cut(s) 156, 346, 815, 1003
Cac8I GCNNGC 2 cut(s) 633, 1257
Cfr13I GGNCC 2 cut(s) 534, 672
CseI GACGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 742, 1180
CviAII CATG 5 cut(s) 58, 580, 604, 922, 1060
CviQI GTAC 2 cut(s) 742, 1180
DdeI CTNAG 1 cut(s) 818
DpnI GATC 7 cut(s) 36, 419, 519, 540, 768, 1142, 1373
DpnII GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
DraIII CACNNNGTG 1 cut(s) 727
Eco130I CCWWGG 3 cut(s) 675, 1045, 1263
Eco24I GRGCYC 1 cut(s) 661
Eco31I GGTCTC 1 cut(s) 902
Eco32I GATATC 2 cut(s) 322, 601
Eco47I GGWCC 2 cut(s) 534, 672
Eco57I CTGAAG 3 cut(s) 49, 662, 1236
EcoRII CCWGG 2 cut(s) 653, 668
EcoRV GATATC 2 cut(s) 322, 601
EcoT14I CCWWGG 3 cut(s) 675, 1045, 1263
EcoT22I ATGCAT 2 cut(s) 184, 362
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 3 cut(s) 675, 1045, 1263
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 5 cut(s) 61, 583, 607, 925, 1063
FatI CATG 5 cut(s) 57, 579, 603, 921, 1059
FbaI TGATCA 1 cut(s) 417
Fnu4HI GCNGC 6 cut(s) 70, 719, 796, 940, 1131, 1260
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 6 cut(s) 70, 719, 796, 940, 1131, 1260
FspBI CTAG 2 cut(s) 681, 845
GluI GCNGC 6 cut(s) 70, 719, 796, 940, 1131, 1260
GsaI CCCAGC 1 cut(s) 1137
GsuI CTGGAG 1 cut(s) 645
HaeIII GGCC 2 cut(s) 26, 425
HapII CCGG 2 cut(s) 626, 1363
HgaI GACGC 1 cut(s) 337
Hin1II CATG 5 cut(s) 61, 583, 607, 925, 1063
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 2 cut(s) 92, 449
HinfI GANTC 5 cut(s) 439, 614, 749, 822, 1041
HpaI GTTAAC 1 cut(s) 862
HpaII CCGG 2 cut(s) 626, 1363
HphI GGTGA 3 cut(s) 278, 329, 819
Hpy166II GTNNAC 2 cut(s) 744, 862
Hpy188I TCNGA 4 cut(s) 291, 312, 954, 1170
Hpy188III TCNNGA 5 cut(s) 325, 753, 1088, 1237, 1363
Hpy8I GTNNAC 2 cut(s) 744, 862
Hpy99I CGWCG 2 cut(s) 228, 1350
HpyAV CCTTC 6 cut(s) 105, 200, 240, 257, 370, 1349
HpyCH4III ACNGT 3 cut(s) 15, 238, 341
HpyCH4IV ACGT 1 cut(s) 740
HpyCH4V TGCA 9 cut(s) 182, 244, 360, 398, 635, 803, 942, 1228, 1246
HpyF10VI GCNNNNNNNGC 2 cut(s) 727, 1252
HpyF3I CTNAG 1 cut(s) 818
HpySE526I ACGT 1 cut(s) 740
Hsp92II CATG 5 cut(s) 61, 583, 607, 925, 1063
Kpn2I TCCGGA 1 cut(s) 1362
Ksp22I TGATCA 1 cut(s) 417
KspAI GTTAAC 1 cut(s) 862
Kzo9I GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
LmnI GCTCC 2 cut(s) 628, 664
Lsp1109I GCAGC 5 cut(s) 81, 730, 782, 926, 1246
LweI GCATC 3 cut(s) 34, 857, 1351
MaeI CTAG 2 cut(s) 681, 845
MaeII ACGT 1 cut(s) 740
MaeIII GTNAC 4 cut(s) 266, 615, 683, 944
MalI GATC 7 cut(s) 36, 419, 519, 540, 768, 1142, 1373
MbiI CCGCTC 1 cut(s) 79
MboI GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
MboII GAAGA 9 cut(s) 44, 55, 120, 583, 696, 977, 1150, 1186, 1378
MfeI CAATTG 1 cut(s) 1095
MflI RGATCY 1 cut(s) 1140
MhlI GDGCHC 2 cut(s) 661, 732
MluCI AATT 8 cut(s) 161, 191, 574, 734, 1095, 1186, 1204, 1384
MlyI GAGTC 2 cut(s) 623, 1035
MnlI CCTC 9 cut(s) 16, 98, 195, 265, 657, 686, 805, 1066, 1176
Mph1103I ATGCAT 2 cut(s) 184, 362
MroI TCCGGA 1 cut(s) 1362
MroXI GAANNNNTTC 1 cut(s) 575
MseI TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
MspA1I CMGCKG 2 cut(s) 175, 1133
MspI CCGG 2 cut(s) 626, 1363
MspR9I CCNGG 2 cut(s) 655, 670
MunI CAATTG 1 cut(s) 1095
Mva1269I GAATGC 2 cut(s) 184, 496
MvaI CCWGG 2 cut(s) 655, 670
MwoI GCNNNNNNNGC 2 cut(s) 727, 1252
NdeII GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
NlaIII CATG 5 cut(s) 61, 583, 607, 925, 1063
NlaIV GGNNCC 2 cut(s) 660, 1361
NmuCI GTSAC 3 cut(s) 266, 615, 944
NsiI ATGCAT 2 cut(s) 184, 362
NspI RCATGY 2 cut(s) 61, 925
PasI CCCWGGG 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 304
PctI GAATGC 2 cut(s) 184, 496
PdmI GAANNNNTTC 1 cut(s) 575
PfeI GAWTC 3 cut(s) 439, 749, 822
PkrI GCNGC 6 cut(s) 71, 720, 797, 941, 1132, 1261
PleI GAGTC 2 cut(s) 622, 1035
PpsI GAGTC 2 cut(s) 622, 1035
Ppu21I YACGTR 1 cut(s) 741
PshBI ATTAAT 2 cut(s) 363, 1023
PsiI TTATAA 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 653, 668
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 2 cut(s) 653, 668
PspN4I GGNNCC 2 cut(s) 660, 1361
PspPI GGNCC 2 cut(s) 534, 672
PstI CTGCAG 1 cut(s) 944
PsuI RGATCY 1 cut(s) 1140
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 2 cut(s) 743, 1181
RsaNI GTAC 2 cut(s) 742, 1180
SaqAI TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
SatI GCNGC 6 cut(s) 70, 719, 796, 940, 1131, 1260
Sau3AI GATC 7 cut(s) 34, 417, 517, 538, 766, 1140, 1371
Sau96I GGNCC 2 cut(s) 534, 672
SchI GAGTC 2 cut(s) 623, 1035
ScrFI CCNGG 2 cut(s) 655, 670
SduI GDGCHC 2 cut(s) 661, 732
SfaNI GCATC 3 cut(s) 34, 857, 1351
SfcI CTRYAG 2 cut(s) 688, 940
SinI GGWCC 2 cut(s) 534, 672
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 8 cut(s) 161, 191, 574, 734, 1095, 1186, 1204, 1384
SsiI CCGC 2 cut(s) 77, 1131
SspMI CTAG 2 cut(s) 681, 845
StyD4I CCNGG 2 cut(s) 653, 668
StyI CCWWGG 3 cut(s) 675, 1045, 1263
TaaI ACNGT 3 cut(s) 15, 238, 341
TaiI ACGT 1 cut(s) 743
TaqI TCGA 5 cut(s) 223, 233, 367, 837, 1345
TaqII GACCGA 1 cut(s) 551
TasI AATT 8 cut(s) 161, 191, 574, 734, 1095, 1186, 1204, 1384
TauI GCSGC 1 cut(s) 1133
TfiI GAWTC 3 cut(s) 439, 749, 822
Tru1I TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
Tru9I TTAA 8 cut(s) 51, 363, 552, 590, 861, 869, 905, 1023
TscAI CASTG 4 cut(s) 156, 346, 822, 1010
TseFI GTSAC 3 cut(s) 266, 615, 944
TseI GCWGC 5 cut(s) 69, 718, 795, 939, 1259
Tsp45I GTSAC 3 cut(s) 266, 615, 944
TspDTI ATGAA 6 cut(s) 461, 503, 568, 768, 927, 1288
TspRI CASTG 4 cut(s) 156, 346, 822, 1010
VpaK11BI GGWCC 2 cut(s) 534, 672
VspI ATTAAT 2 cut(s) 363, 1023
XapI RAATTY 4 cut(s) 161, 574, 734, 1204
XceI RCATGY 2 cut(s) 61, 925
XmnI GAANNNNTTC 1 cut(s) 575
XspI CTAG 2 cut(s) 681, 845
Zsp2I ATGCAT 2 cut(s) 184, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.