FvH4_7g26960

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015180
Physical Location & Seq
Reverse (-)
20238223 .. 20241800
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26960.t1

Sequence Viewer

Length: 1332 bp
CCCAGAGTAAAACTTTTCAGTTATTTATTTTCATTTTCAGGAAATTATTTAGTTTCCTTCATTCTCAGGTTTCTTCATTCATCAACAGTCGCAACAAACAACAAACTCTCATCACCGGCCTTTCTCTTCTTCCATTTTAATTTCCCACTCTCATTTCTCCTCCGTGATCGTTTCGATTTTCTTATCCGACTGCATACCATACTCGCGCCCAATGCGCGTAGCCGAACAACCGCTATAGCAATCACCATGGAAAACGTCGTCATCAAAGAGAACGGAGTGTGCTCGTCGGAGTCGGTCAATGGTAGCCGCTTTGTCTGGAGTGCTAAGGAGTCCGATTCTTCCTCTGCCGACCATCTCGTCGTCATGGTTCACGGAATAATGGGAAGTCAGTCAGATTGGAAGTTTGGAGCAGAGCAGTTTGTTAAAATGCTCCCCGACAAAGTTCTTGTTCATTGTAGTGAACGGAATGCCTCTATACTGACCCTGGATGGTGTGGATGTAATGGGAGAGCGCTTGGCAATAGAGGTTGGCGAACTGGTTCAAAGAAAGCCAAATTTACGTAAGATCTCATTCGTGGCACATTCTGTGGGAGGATTAGTGGCAAGATATGCAATTGGGAGACTATATAGACCCCCTAAGGGTGAAAATACAGACAATTCACATCCTAATGGCTGTGAAGAGGATTCAAAGAGTACGATATGTGGCTTAGAGCCTATCAATTTCATCACTGTTGCTACACCACATCTTGGTTCTAGGGGTAATAAGCAGGTACCATTCCTTTTTGGTGTACCTGCCTTCGAAAAACTTGCTAGTGCAGTTATCCATTTGATATTTAGGAGAACAGGTCGCCATTTGTTTCTTAATGACGATGATGATGGAAAGCCTCCGCTGCTTAAACGAATGATAGAAGACTGTGATGGATGTTATTTCATGTCGGCATTGCGTAGTTTCAGACGTAGGGTGGCATATTCAAATGTGGGATATGACCATATTGTCGGCTGGAGAACATCAAGCATTAGACGTAATAGTGAACTGCCAAAGTGGGAGGATACTGTTGATGAAAAATATCCACATATTGTATATGAAGAACACTGCAAGGCCTATGATGCTGAGCAGTGTGAGCCTACTTCAGCGGATGGCAGTGACTGTGACCAGCTTGAAGGGGAACTAGTGACAGGCCTTTCTCGTGTGTCCTGGGAAAAAGTAGATGTTAGTTTCCACTGCAGCAAACAGAGATTTGCTGCTCATAGTGTCATTCAGGTAAAAGATCAGACTGCACATAAAGAAGGCGAAGATGTTATCCGTCATATGATTGATCATTTCCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

444

Amino Acids

50.0

Weight (kDa)

6.93

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 114 - 336 3.5e-67 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51180 AT5G51180
fragaria_vesca FvH4_7g26960
malus_domestica MD01G1177400.v1.1 MD07G1249100.v1.1
prunus_persica Prupe.2G272700_v2.0.a1
pyrus_communis pycom01g18790 pycom07g22230
rosa_chinensis RchiOBHm_Chr1g0373671
rosa_laevigata RLG00000026808
rosa_roxburghii Rroxscaffold_4G00284070
rosa_rugosa Rorug01G0378900
rosa_samantha Rh1AG388300 Rh1CG365600 Rh1DG383100
rosa_wichuraiana Rw1G034690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 356, 992
Acc36I ACCTGC 2 cut(s) 757, 799
Acc65I GGTACC 1 cut(s) 769
AccB1I GGYRCC 1 cut(s) 769
AccB7I CCANNNNNTGG 1 cut(s) 746
AccII CGCG 2 cut(s) 206, 217
AciI CCGC 4 cut(s) 231, 307, 887, 1133
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 1113
AfaI GTAC 3 cut(s) 694, 771, 789
AfeI AGCGCT 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 638, 746
AgsI TTSAA 4 cut(s) 542, 687, 972, 1160
AhlI ACTAGT 1 cut(s) 1168
AjnI CCWGG 2 cut(s) 483, 1193
AluBI AGCT 1 cut(s) 1156
AluI AGCT 1 cut(s) 1156
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 1146
Aor51HI AGCGCT 1 cut(s) 512
AoxI GGCC 3 cut(s) 117, 1098, 1177
ApeKI GCWGC 3 cut(s) 889, 1224, 1241
ApoI RAATTY 1 cut(s) 553
Asp700I GAANNNNTTC 1 cut(s) 537
Asp718I GGTACC 1 cut(s) 769
AspLEI GCGC 3 cut(s) 208, 217, 513
AsuHPI GGTGA 3 cut(s) 105, 235, 653
AsuII TTCGAA 1 cut(s) 798
AxyI CCTNAGG 1 cut(s) 636
BanI GGYRCC 1 cut(s) 769
BauI CACGAG 1 cut(s) 1185
BbsI GAAGAC 1 cut(s) 915
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 876, 1228, 1236
BccI CCATC 5 cut(s) 360, 482, 869, 911, 1130
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 2 cut(s) 485, 1195
BciVI GTATCC 1 cut(s) 1042
BclI TGATCA 1 cut(s) 1315
BcoDI GTCTC 1 cut(s) 613
BcuI ACTAGT 1 cut(s) 1168
BfaI CTAG 4 cut(s) 753, 810, 1169, 1330
BfmI CTRYAG 2 cut(s) 234, 1222
BfoI RGCGCY 1 cut(s) 514
BfuAI ACCTGC 2 cut(s) 757, 799
BfuI GTATCC 1 cut(s) 1042
BglII AGATCT 1 cut(s) 564
BisI GCNGC 4 cut(s) 307, 890, 1225, 1242
BlpI GCTNAGC 1 cut(s) 1110
BlsI GCNGC 4 cut(s) 308, 891, 1226, 1243
Bme1390I CCNGG 2 cut(s) 485, 1195
BmiI GGNNCC 1 cut(s) 771
BmrFI CCNGG 2 cut(s) 485, 1195
BmsI GCATC 1 cut(s) 1096
BpiI GAAGAC 1 cut(s) 915
BpmI CTGGAG 2 cut(s) 337, 1021
Bpu10I CCTNAGC 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 246, 483, 1194
BsaXI ACNNNNNCTCC 4 cut(s) 582, 612, 829, 859
Bsc4I CCNNNNNNNGG 2 cut(s) 638, 746
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 540
Bse21I CCTNAGG 1 cut(s) 636
Bse3DI GCAATG 1 cut(s) 938
BseBI CCWGG 2 cut(s) 485, 1195
BseDI CCNNGG 3 cut(s) 246, 483, 1194
BseGI GGATG 5 cut(s) 493, 502, 661, 926, 1141
BseLI CCNNNNNNNGG 2 cut(s) 638, 746
BseMI GCAATG 1 cut(s) 938
BseMII CTCAG 2 cut(s) 79, 1101
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 3 cut(s) 876, 1228, 1236
BsgI GTGCAG 2 cut(s) 834, 1260
Bsh1236I CGCG 2 cut(s) 206, 217
BshFI GGCC 3 cut(s) 119, 1100, 1179
BshNI GGYRCC 1 cut(s) 769
BsiHKAI GWGCWC 1 cut(s) 284
BsiSI CCGG 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 638, 746
BsmAI GTCTC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 3 cut(s) 119, 1100, 1179
Bsp119I TTCGAA 1 cut(s) 798
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 4 cut(s) 166, 564, 1267, 1315
Bsp1720I GCTNAGC 1 cut(s) 1110
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 4 cut(s) 231, 307, 887, 1133
BspANI GGCC 3 cut(s) 119, 1100, 1179
BspCNI CTCAG 2 cut(s) 78, 1102
BspFNI CGCG 2 cut(s) 206, 217
BspLI GGNNCC 1 cut(s) 771
BspMAI CTGCAG 1 cut(s) 1226
BspMI ACCTGC 2 cut(s) 757, 799
BspT104I TTCGAA 1 cut(s) 798
BspT107I GGYRCC 1 cut(s) 769
BsrDI GCAATG 1 cut(s) 938
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 1 cut(s) 540
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 246, 483, 1194
BssMI GATC 4 cut(s) 166, 564, 1267, 1315
BssSI CACGAG 1 cut(s) 1185
BssT1I CCWWGG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 1185
Bst2UI CCWGG 2 cut(s) 485, 1195
Bst4CI ACNGT 5 cut(s) 88, 730, 914, 1054, 1148
Bst6I CTCTTC 2 cut(s) 131, 672
BstAPI GCANNNNNTGC 1 cut(s) 608
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 798
BstDEI CTNAG 5 cut(s) 65, 324, 636, 706, 1110
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 5 cut(s) 493, 502, 661, 926, 1141
BstFNI CGCG 2 cut(s) 206, 217
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 3 cut(s) 208, 217, 513
BstKTI GATC 4 cut(s) 169, 567, 1270, 1318
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 4 cut(s) 166, 564, 1267, 1315
BstMWI GCNNNNNNNGC 6 cut(s) 212, 214, 608, 889, 1106, 1120
BstNI CCWGG 2 cut(s) 485, 1195
BstSCI CCNGG 2 cut(s) 483, 1193
BstSFI CTRYAG 2 cut(s) 234, 1222
BstSNI TACGTA 1 cut(s) 560
BstUI CGCG 2 cut(s) 206, 217
BstV1I GCAGC 3 cut(s) 876, 1228, 1236
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 636
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 3 cut(s) 119, 1100, 1179
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 5 cut(s) 493, 502, 661, 926, 1141
BtsI GCAGTG 4 cut(s) 1090, 1121, 1147, 1219
BtsIMutI CAGTG 5 cut(s) 726, 1090, 1121, 1147, 1219
BveI ACCTGC 2 cut(s) 757, 799
CaiI CAGNNNCTG 1 cut(s) 1146
CfoI GCGC 3 cut(s) 208, 217, 513
Cfr10I RCCGGY 1 cut(s) 115
Csp6I GTAC 3 cut(s) 693, 770, 788
CviAII CATG 3 cut(s) 247, 364, 931
CviQI GTAC 3 cut(s) 693, 770, 788
DdeI CTNAG 5 cut(s) 65, 324, 636, 706, 1110
DpnI GATC 4 cut(s) 168, 566, 1269, 1317
DpnII GATC 4 cut(s) 166, 564, 1267, 1315
DrdI GACNNNNNNGTC 2 cut(s) 356, 992
DseDI GACNNNNNNGTC 2 cut(s) 356, 992
Eam1104I CTCTTC 2 cut(s) 131, 672
EarI CTCTTC 2 cut(s) 131, 672
Eco105I TACGTA 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 246
Eco147I AGGCCT 2 cut(s) 1100, 1179
Eco47III AGCGCT 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 1113
Eco81I CCTNAGG 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 483, 1193
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 3 cut(s) 250, 367, 934
FatI CATG 3 cut(s) 246, 363, 930
FauNDI CATATG 1 cut(s) 1308
FbaI TGATCA 1 cut(s) 1315
Fnu4HI GCNGC 4 cut(s) 307, 890, 1225, 1242
FokI GGATG 5 cut(s) 500, 509, 648, 933, 1148
Fsp4HI GCNGC 4 cut(s) 307, 890, 1225, 1242
FspBI CTAG 4 cut(s) 753, 810, 1169, 1330
GlaI GCGC 3 cut(s) 207, 216, 512
GluI GCNGC 4 cut(s) 307, 890, 1225, 1242
GsuI CTGGAG 2 cut(s) 337, 1021
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 3 cut(s) 119, 1100, 1179
HapII CCGG 1 cut(s) 116
HhaI GCGC 3 cut(s) 208, 217, 513
Hin1II CATG 3 cut(s) 250, 367, 934
Hin6I GCGC 3 cut(s) 206, 215, 511
HinP1I GCGC 3 cut(s) 206, 215, 511
HinfI GANTC 4 cut(s) 290, 329, 335, 683
HpaII CCGG 1 cut(s) 116
HphI GGTGA 3 cut(s) 105, 235, 653
Hpy166II GTNNAC 4 cut(s) 370, 461, 788, 1031
Hpy188I TCNGA 6 cut(s) 188, 289, 334, 394, 953, 1272
Hpy188III TCNNGA 2 cut(s) 39, 316
Hpy8I GTNNAC 4 cut(s) 370, 461, 788, 1031
Hpy99I CGWCG 3 cut(s) 260, 289, 362
HpyAV CCTTC 4 cut(s) 67, 805, 1154, 1280
HpyCH4III ACNGT 5 cut(s) 88, 730, 914, 1054, 1148
HpyCH4IV ACGT 4 cut(s) 255, 559, 955, 1021
HpyCH4V TGCA 6 cut(s) 193, 611, 815, 1095, 1224, 1277
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 214, 608, 889, 1106, 1120
HpyF3I CTNAG 5 cut(s) 65, 324, 636, 706, 1110
HpySE526I ACGT 4 cut(s) 255, 559, 955, 1021
Hsp92II CATG 3 cut(s) 250, 367, 934
HspAI GCGC 3 cut(s) 206, 215, 511
KpnI GGTACC 1 cut(s) 773
Ksp22I TGATCA 1 cut(s) 1315
Kzo9I GATC 4 cut(s) 166, 564, 1267, 1315
LmnI GCTCC 2 cut(s) 407, 435
Lsp1109I GCAGC 3 cut(s) 876, 1228, 1236
LweI GCATC 1 cut(s) 1096
MaeI CTAG 4 cut(s) 753, 810, 1169, 1330
MaeII ACGT 4 cut(s) 255, 559, 955, 1021
MaeIII GTNAC 3 cut(s) 1142, 1148, 1171
MalI GATC 4 cut(s) 168, 566, 1269, 1317
MboI GATC 4 cut(s) 166, 564, 1267, 1315
MboII GAAGA 8 cut(s) 65, 118, 121, 330, 689, 920, 1097, 1304
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 6 cut(s) 43, 139, 553, 612, 655, 718
MlyI GAGTC 2 cut(s) 299, 338
MmeI TCCRAC 2 cut(s) 211, 267
MnlI CCTC 8 cut(s) 170, 352, 481, 517, 584, 673, 894, 1039
MroXI GAANNNNTTC 1 cut(s) 537
MseI TTAA 4 cut(s) 138, 423, 861, 894
MslI CAYNNNNRTG 2 cut(s) 456, 666
MspA1I CMGCKG 2 cut(s) 889, 1133
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 2 cut(s) 485, 1195
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 2 cut(s) 485, 1195
MvnI CGCG 2 cut(s) 206, 217
MwoI GCNNNNNNNGC 6 cut(s) 212, 214, 608, 889, 1106, 1120
NcoI CCATGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 1308
NdeII GATC 4 cut(s) 166, 564, 1267, 1315
NlaIII CATG 3 cut(s) 250, 367, 934
NlaIV GGNNCC 1 cut(s) 771
NmuCI GTSAC 3 cut(s) 1142, 1148, 1171
NspV TTCGAA 1 cut(s) 798
PceI AGGCCT 2 cut(s) 1100, 1179
PctI GAATGC 1 cut(s) 472
PdmI GAANNNNTTC 1 cut(s) 537
PfeI GAWTC 2 cut(s) 335, 683
PflMI CCANNNNNTGG 1 cut(s) 746
PkrI GCNGC 4 cut(s) 308, 891, 1226, 1243
PleI GAGTC 2 cut(s) 298, 337
PpsI GAGTC 2 cut(s) 298, 337
Ppu21I YACGTR 1 cut(s) 560
Psp6I CCWGG 2 cut(s) 483, 1193
PspGI CCWGG 2 cut(s) 483, 1193
PspN4I GGNNCC 1 cut(s) 771
PstI CTGCAG 1 cut(s) 1226
PstNI CAGNNNCTG 1 cut(s) 1146
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 3 cut(s) 694, 771, 789
RsaNI GTAC 3 cut(s) 693, 770, 788
RseI CAYNNNNRTG 2 cut(s) 456, 666
SaqAI TTAA 4 cut(s) 138, 423, 861, 894
SatI GCNGC 4 cut(s) 307, 890, 1225, 1242
Sau3AI GATC 4 cut(s) 166, 564, 1267, 1315
SchI GAGTC 2 cut(s) 299, 338
ScrFI CCNGG 2 cut(s) 485, 1195
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 1 cut(s) 1096
SfcI CTRYAG 2 cut(s) 234, 1222
SfuI TTCGAA 1 cut(s) 798
SmiMI CAYNNNNRTG 2 cut(s) 456, 666
SnaBI TACGTA 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 1168
Sse9I AATT 6 cut(s) 43, 139, 553, 612, 655, 718
SseBI AGGCCT 2 cut(s) 1100, 1179
SsiI CCGC 4 cut(s) 231, 307, 887, 1133
SspMI CTAG 4 cut(s) 753, 810, 1169, 1330
StuI AGGCCT 2 cut(s) 1100, 1179
StyD4I CCNGG 2 cut(s) 483, 1193
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 5 cut(s) 88, 730, 914, 1054, 1148
TaiI ACGT 4 cut(s) 258, 562, 958, 1024
TaqI TCGA 2 cut(s) 174, 798
TaqII GACCGA 1 cut(s) 283
TasI AATT 6 cut(s) 43, 139, 553, 612, 655, 718
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 2 cut(s) 335, 683
Tru1I TTAA 4 cut(s) 138, 423, 861, 894
Tru9I TTAA 4 cut(s) 138, 423, 861, 894
TscAI CASTG 5 cut(s) 733, 1097, 1121, 1147, 1226
TseFI GTSAC 3 cut(s) 1142, 1148, 1171
TseI GCWGC 3 cut(s) 889, 1224, 1241
Tsp45I GTSAC 3 cut(s) 1142, 1148, 1171
TspDTI ATGAA 9 cut(s) 21, 49, 65, 69, 440, 712, 919, 1074, 1098
TspGWI ACGGA 5 cut(s) 152, 288, 387, 478, 1292
TspRI CASTG 5 cut(s) 733, 1097, 1121, 1147, 1226
Van91I CCANNNNNTGG 1 cut(s) 746
XapI RAATTY 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 537
XspI CTAG 4 cut(s) 753, 810, 1169, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.