pycom07g22230

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015180
Physical Location & Seq
Reverse (-)
23644687 .. 23647510
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22230.9

Sequence Viewer

Length: 1110 bp
ATGCCCGTAGCCGAACGGCGGTCATCTGCAGCCATGGAAAACGGCGTCGTAGAGAACGGTGTCTGCTCGTCGGAGTCGCCTACTGGGAGTCGCTACGTTTGGAGCTCCAAGGAGTCCGAATATTCTTCCGCTGATCATCTCGTTGTCATGGTCCATGGAATCATGGGAACTGCCGCGGATTGGAAGTTTGGGGCGGAGCAATTTGTTAAAACACTTCCGGACAAAGTTATTGTTCATTGTAGTGAACGGAATGGCTCTAGGCTGACTCTAGATGGTGTGGATGTAATGGGGGAGCGATTGGCAGAGGAGGTCATCGAACTGACTCGAAAAAAACCTAACCTACGAAAGATCTCATTTATTGGACATTCTGTTGGAGGATTAGTCGCAAGATATGCAATTGGGAGGCTATATAGACCCGGTCCCTCTAAAAGTGAGAATACGGAACATTCATCTCCTAATGGATGTGAAGAGGATGCAAGGAGTACATTAGCTGGCTTGGAGCCTTTGAACTTTATAACTGTTGCCACACCTCATCTTGGATCAAGGGGTAACAAGCAGGTGCCATTTCTTTTTGGTGTACCTGCCTTTGAAAGACTTGCTAGTGCGGTTATTCATTTGATATTTAGGAGAACAGGTCGGCATCTTTTTCTTAATGATGACGATGATGGGAAGCCTCCACTGCTTAAACGAATGATAGAAGATTGTGATGGTTGTTATTTCATGTCTGCATTGCGTAGTTTTAAGCGGAGGGTGGTGTATTCAAACGTGGGCTATGACCATATTGTTGGCTGGAAAACATCATGTATTAGACGTAATAGCGAACTTCCAAAGTGGGAAAATACTGTTGACGAAAAATATCCGCATATTGTTTATGAAGAACACTGCAAGGCCTATGATGCTGAGCAGTGTGAGCCTGCTTCAGCAGAAGATGGTGGCTCTGGCGAGCTTGAAGAGGAACTATTGAAAGGCCTATCTCGCGTGTCTTGGGAAAAAGTAGATGTTAGTTTCCACAGCAGCAGACGGAGATTTGCTGCTCATAGTGTCATTCAGGTCAAAGATCAAAGCGTACATATAGAAGGTGAAGATGTCATACGCCATCCGAGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

370

Amino Acids

41.2

Weight (kDa)

6.22

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51180 AT5G51180
fragaria_vesca FvH4_7g26960
malus_domestica MD01G1177400.v1.1 MD07G1249100.v1.1
prunus_persica Prupe.2G272700_v2.0.a1
pyrus_communis pycom01g18790 pycom07g22230
rosa_chinensis RchiOBHm_Chr1g0373671
rosa_laevigata RLG00000026808
rosa_roxburghii Rroxscaffold_4G00284070
rosa_rugosa Rorug01G0378900
rosa_samantha Rh1AG388300 Rh1CG365600 Rh1DG383100
rosa_wichuraiana Rw1G034690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AarI CACCTGC 1 cut(s) 547
Acc36I ACCTGC 2 cut(s) 547, 589
AccB1I GGYRCC 1 cut(s) 559
AccII CGCG 2 cut(s) 176, 978
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 8 cut(s) 19, 129, 174, 176, 194, 605, 745, 860
AclWI GGATC 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 903
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 484, 579, 1068
AfiI CCNNNNNNNGG 3 cut(s) 18, 180, 536
AgsI TTSAA 5 cut(s) 508, 590, 762, 950, 964
AluBI AGCT 3 cut(s) 105, 491, 946
AluI AGCT 3 cut(s) 105, 491, 946
Alw21I GWGCWC 1 cut(s) 107
AlwI GGATC 1 cut(s) 547
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 2 cut(s) 888, 967
ApeKI GCWGC 3 cut(s) 29, 1014, 1031
AspS9I GGNCC 2 cut(s) 151, 419
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 1 cut(s) 1091
AvaII GGWCC 2 cut(s) 151, 419
BanI GGYRCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 1074, 1106
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 41, 1018, 1026
BccI CCATC 5 cut(s) 266, 659, 701, 923, 1104
BceAI ACGGC 2 cut(s) 32, 58
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 1 cut(s) 417
BfaI CTAG 3 cut(s) 258, 269, 600
BfmI CTRYAG 1 cut(s) 27
BfuAI ACCTGC 2 cut(s) 547, 589
BglII AGATCT 1 cut(s) 348
BisI GCNGC 4 cut(s) 30, 174, 1015, 1032
BlpI GCTNAGC 1 cut(s) 900
BlsI GCNGC 4 cut(s) 31, 175, 1016, 1033
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 2 cut(s) 151, 419
BmgT120I GGNCC 2 cut(s) 151, 419
BmiI GGNNCC 3 cut(s) 421, 501, 561
BmrFI CCNGG 1 cut(s) 417
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 3 cut(s) 463, 649, 886
BmuI ACTGGG 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 900
BpuMI CCSGG 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 4 cut(s) 33, 108, 154, 174
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 619, 649
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 180, 536
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 728
BseAI TCCGGA 1 cut(s) 217
BseDI CCNNGG 4 cut(s) 33, 108, 154, 174
BseGI GGATG 4 cut(s) 286, 467, 478, 1096
BseLI CCNNNNNNNGG 3 cut(s) 18, 180, 536
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 3 cut(s) 41, 1018, 1026
Bsh1236I CGCG 2 cut(s) 176, 978
BshFI GGCC 2 cut(s) 890, 969
BshNI GGYRCC 1 cut(s) 559
BsiHKAI GWGCWC 1 cut(s) 107
BsiSI CCGG 2 cut(s) 218, 417
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 3 cut(s) 18, 180, 536
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 2 cut(s) 890, 969
Bsp1286I GDGCHC 1 cut(s) 107
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 4 cut(s) 133, 348, 539, 1057
Bsp1720I GCTNAGC 1 cut(s) 900
Bsp19I CCATGG 2 cut(s) 33, 154
BspACI CCGC 8 cut(s) 19, 129, 174, 176, 194, 605, 745, 860
BspANI GGCC 2 cut(s) 890, 969
BspCNI CTCAG 1 cut(s) 892
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 2 cut(s) 176, 978
BspLI GGNNCC 3 cut(s) 421, 501, 561
BspMAI CTGCAG 1 cut(s) 31
BspMI ACCTGC 2 cut(s) 547, 589
BspPI GGATC 1 cut(s) 547
BspT107I GGYRCC 1 cut(s) 559
BsrDI GCAATG 1 cut(s) 728
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 4 cut(s) 33, 108, 154, 174
BssMI GATC 4 cut(s) 133, 348, 539, 1057
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 3 cut(s) 33, 108, 154
Bst4CI ACNGT 3 cut(s) 59, 520, 844
Bst6I CTCTTC 2 cut(s) 462, 945
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 493, 915, 944
BstDEI CTNAG 1 cut(s) 900
BstDSI CCRYGG 3 cut(s) 33, 154, 174
BstF5I GGATG 4 cut(s) 286, 467, 478, 1096
BstFNI CGCG 2 cut(s) 176, 978
BstKTI GATC 4 cut(s) 136, 351, 542, 1060
BstMBI GATC 4 cut(s) 133, 348, 539, 1057
BstMWI GCNNNNNNNGC 6 cut(s) 392, 679, 896, 910, 975, 1101
BstSCI CCNGG 1 cut(s) 415
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 2 cut(s) 176, 978
BstV1I GCAGC 3 cut(s) 41, 1018, 1026
BstX2I RGATCY 1 cut(s) 348
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 2 cut(s) 890, 969
BtgI CCRYGG 3 cut(s) 33, 154, 174
BtsCI GGATG 4 cut(s) 286, 467, 478, 1096
BtsI GCAGTG 3 cut(s) 677, 880, 911
BtsIMutI CAGTG 3 cut(s) 677, 880, 911
BveI ACCTGC 2 cut(s) 547, 589
Cac8I GCNNGC 3 cut(s) 493, 915, 944
Cfr13I GGNCC 2 cut(s) 151, 419
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 483, 578, 1067
CviAII CATG 6 cut(s) 34, 148, 155, 163, 721, 801
CviQI GTAC 3 cut(s) 483, 578, 1067
DdeI CTNAG 1 cut(s) 900
DpnI GATC 4 cut(s) 135, 350, 541, 1059
DpnII GATC 4 cut(s) 133, 348, 539, 1057
Eam1104I CTCTTC 2 cut(s) 462, 945
EarI CTCTTC 2 cut(s) 462, 945
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 3 cut(s) 33, 108, 154
Eco147I AGGCCT 2 cut(s) 890, 969
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 151, 419
Eco53kI GAGCTC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 903
EcoICRI GAGCTC 1 cut(s) 105
EcoT14I CCWWGG 3 cut(s) 33, 108, 154
EcoT38I GRGCYC 1 cut(s) 107
ErhI CCWWGG 3 cut(s) 33, 108, 154
FaeI CATG 6 cut(s) 37, 151, 158, 166, 724, 804
FaqI GGGAC 1 cut(s) 405
FatI CATG 6 cut(s) 33, 147, 154, 162, 720, 800
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 30, 174, 1015, 1032
FokI GGATG 4 cut(s) 293, 474, 485, 1083
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 4 cut(s) 30, 174, 1015, 1032
FspBI CTAG 3 cut(s) 258, 269, 600
GluI GCNGC 4 cut(s) 30, 174, 1015, 1032
HaeIII GGCC 2 cut(s) 890, 969
HapII CCGG 2 cut(s) 218, 417
HgaI GACGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 6 cut(s) 37, 151, 158, 166, 724, 804
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HinfI GANTC 6 cut(s) 74, 88, 113, 159, 265, 322
HpaII CCGG 2 cut(s) 218, 417
HphI GGTGA 1 cut(s) 1091
Hpy166II GTNNAC 3 cut(s) 245, 578, 847
Hpy188I TCNGA 3 cut(s) 73, 118, 1101
Hpy188III TCNNGA 2 cut(s) 218, 269
Hpy8I GTNNAC 3 cut(s) 245, 578, 847
Hpy99I CGWCG 2 cut(s) 50, 73
HpyAV CCTTC 1 cut(s) 1070
HpyCH4III ACNGT 3 cut(s) 59, 520, 844
HpyCH4IV ACGT 3 cut(s) 96, 765, 811
HpyCH4V TGCA 5 cut(s) 29, 395, 476, 728, 885
HpyF10VI GCNNNNNNNGC 6 cut(s) 392, 679, 896, 910, 975, 1101
HpyF3I CTNAG 1 cut(s) 900
HpySE526I ACGT 3 cut(s) 96, 765, 811
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 6 cut(s) 37, 151, 158, 166, 724, 804
Kpn2I TCCGGA 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 133
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 4 cut(s) 133, 348, 539, 1057
LmnI GCTCC 5 cut(s) 102, 110, 196, 292, 499
Lsp1109I GCAGC 3 cut(s) 41, 1018, 1026
LweI GCATC 3 cut(s) 463, 649, 886
MaeI CTAG 3 cut(s) 258, 269, 600
MaeII ACGT 3 cut(s) 96, 765, 811
MaeIII GTNAC 1 cut(s) 548
MalI GATC 4 cut(s) 135, 350, 541, 1059
MboI GATC 4 cut(s) 133, 348, 539, 1057
MboII GAAGA 7 cut(s) 117, 479, 710, 887, 938, 962, 1094
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 3 cut(s) 200, 396, 1105
MlyI GAGTC 5 cut(s) 83, 97, 122, 259, 316
MmeI TCCRAC 2 cut(s) 51, 352
MroI TCCGGA 1 cut(s) 217
MseI TTAA 5 cut(s) 207, 651, 684, 741, 1108
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 2 cut(s) 131, 176
MspI CCGG 2 cut(s) 218, 417
MspR9I CCNGG 1 cut(s) 417
MunI CAATTG 1 cut(s) 396
MvnI CGCG 2 cut(s) 176, 978
MwoI GCNNNNNNNGC 6 cut(s) 392, 679, 896, 910, 975, 1101
NciI CCSGG 1 cut(s) 417
NcoI CCATGG 2 cut(s) 33, 154
NdeII GATC 4 cut(s) 133, 348, 539, 1057
NlaIII CATG 6 cut(s) 37, 151, 158, 166, 724, 804
NlaIV GGNNCC 3 cut(s) 421, 501, 561
PaqCI CACCTGC 1 cut(s) 547
PceI AGGCCT 2 cut(s) 890, 969
PfeI GAWTC 1 cut(s) 159
PflFI GACNNNGTC 1 cut(s) 417
PkrI GCNGC 4 cut(s) 31, 175, 1016, 1033
PleI GAGTC 5 cut(s) 82, 96, 121, 259, 316
PpsI GAGTC 5 cut(s) 82, 96, 121, 259, 316
PsiI TTATAA 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 107
PspN4I GGNNCC 3 cut(s) 421, 501, 561
PspPI GGNCC 2 cut(s) 151, 419
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 348
PsyI GACNNNGTC 1 cut(s) 417
RsaI GTAC 3 cut(s) 484, 579, 1068
RsaNI GTAC 3 cut(s) 483, 578, 1067
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 107
SacII CCGCGG 1 cut(s) 177
SaqAI TTAA 5 cut(s) 207, 651, 684, 741, 1108
SatI GCNGC 4 cut(s) 30, 174, 1015, 1032
Sau3AI GATC 4 cut(s) 133, 348, 539, 1057
Sau96I GGNCC 2 cut(s) 151, 419
SchI GAGTC 5 cut(s) 83, 97, 122, 259, 316
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 3 cut(s) 463, 649, 886
SfcI CTRYAG 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 151, 419
SmiMI CAYNNNNRTG 1 cut(s) 240
Sse9I AATT 3 cut(s) 200, 396, 1105
SseBI AGGCCT 2 cut(s) 890, 969
SsiI CCGC 8 cut(s) 19, 129, 174, 176, 194, 605, 745, 860
SspI AATATT 1 cut(s) 122
SspMI CTAG 3 cut(s) 258, 269, 600
SstI GAGCTC 1 cut(s) 107
StuI AGGCCT 2 cut(s) 890, 969
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 3 cut(s) 33, 108, 154
TaaI ACNGT 3 cut(s) 59, 520, 844
TaiI ACGT 3 cut(s) 99, 768, 814
TaqI TCGA 2 cut(s) 315, 325
TasI AATT 3 cut(s) 200, 396, 1105
TatI WGTACW 1 cut(s) 482
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 5 cut(s) 207, 651, 684, 741, 1108
Tru9I TTAA 5 cut(s) 207, 651, 684, 741, 1108
TscAI CASTG 3 cut(s) 684, 887, 911
TseI GCWGC 3 cut(s) 29, 1014, 1031
TspDTI ATGAA 5 cut(s) 224, 438, 602, 709, 888
TspGWI ACGGA 3 cut(s) 262, 455, 1036
TspRI CASTG 3 cut(s) 684, 887, 911
Tth111I GACNNNGTC 1 cut(s) 417
VpaK11BI GGWCC 2 cut(s) 151, 419
XbaI TCTAGA 1 cut(s) 268
XspI CTAG 3 cut(s) 258, 269, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.