MD01G1177400.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015180
Physical Location & Seq
Reverse (-)
27807051 .. 27810696
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1177400.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGCCCGTAGCCGAACGGCGGTCAGTAGCAGCTATGGAAAACGGCATCGTAGAGAACGGAGTCTGCTCCTCGGAGTCGGCTAACGGGAATCGCGACGTCTGGAGCTCGAAGGAGTCGGAATCTTCCTCCGCTGATCATCTTGTCGTCATGGTCCATGGAATTATGGGAACTGCCGCGGATTGGAAGTTTGGGGCGGAGCAATTTGTTAAAACACTTCCAGATAAAGTTATTGTTCATTGTAGTGAACGGAATGTCTCTAGGTTGACTCTAGATGGTGTGGATGTGATGGGGGAGCGATTGGCAGAGGAGGTTATCGAACTGACTCAAAAAAAGCCTAATCTACGTAAGGTCTCATTTATTGGACATTCTGTCGGAGGATTAGTGGCAAGATATGCAATTGGGAGGCTATATAGACCCCCTAAAAGTGACAATACGGAGCATTCATCTCCTAATGGGCGTGAAGAGGATGCAAGGAGTACATTAGCTGGCTTGGAGCCTATGAACTTTATTACTGTTGCTACACCTCATCTTGGATCAAGGGGTAACAAGCAGGTACCATTCCTTTTTGGTGTGCCTGCCTTTGAAAAACTTGCTAGTGCAGTTATTCATTTGATATTTAGGAGGACTGGTCGGCATCTTTTTCTTAATGATGATGACGATGGGAAGCCTCCACTGCTTAAACGCATGATAGAAGATTCTGATGGTTGTTATTTCATGTCTGCATTGCGTAGTTTTAAGCGAAGGGTGGTGTATTCAAATGTGGGCAATGACCATATTGTCGGCTGGAAAACATCATGTATTAGACGTAACAGTGAACTTCCAAAGTGGGAAAATACTGTTGACGAAAAATATCCGCATATTGTTTATGAAGAACACTGCAAGGCCTATGACGCTGAGCAGTGTGAGCCTACTTCAGCAGAAGATGGTGGCTCTGACGAGCTTGAAGAGGAACTATTGAAAGGCCTATCTCGCGTGTCCTGGGAAAAAGTAGATGTTAGTTTCCACAGCAGCAGACAGAGATTTGCTGCTCATAGTGTCATTCAGGTCAAAAATAAAAGTGTACATATAGAAGGTGAAGATGTCATCCGCCATATAATGGATCATTTCCTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

372

Amino Acids

41.58

Weight (kDa)

6.16

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 42 - 264 2.5e-69 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51180 AT5G51180
fragaria_vesca FvH4_7g26960
malus_domestica MD01G1177400.v1.1 MD07G1249100.v1.1
prunus_persica Prupe.2G272700_v2.0.a1
pyrus_communis pycom01g18790 pycom07g22230
rosa_chinensis RchiOBHm_Chr1g0373671
rosa_laevigata RLG00000026808
rosa_roxburghii Rroxscaffold_4G00284070
rosa_rugosa Rorug01G0378900
rosa_samantha Rh1AG388300 Rh1CG365600 Rh1DG383100
rosa_wichuraiana Rw1G034690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 776
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 541
Acc65I GGTACC 1 cut(s) 553
AccB1I GGYRCC 1 cut(s) 553
AccB7I CCANNNNNTGG 1 cut(s) 1096
AccII CGCG 3 cut(s) 93, 176, 972
AciI CCGC 7 cut(s) 19, 129, 174, 176, 194, 854, 1087
AclWI GGATC 2 cut(s) 541, 1107
AcuI CTGAAG 1 cut(s) 897
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 3 cut(s) 478, 555, 1062
AfiI CCNNNNNNNGG 4 cut(s) 18, 180, 530, 1096
AgsI TTSAA 4 cut(s) 584, 756, 944, 958
AhdI GACNNNNNGTC 1 cut(s) 368
AjnI CCWGG 1 cut(s) 977
AluBI AGCT 4 cut(s) 32, 105, 485, 940
AluI AGCT 4 cut(s) 32, 105, 485, 940
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 2 cut(s) 259, 355
AlwI GGATC 2 cut(s) 541, 1107
AoxI GGCC 2 cut(s) 882, 961
ApeKI GCWGC 3 cut(s) 29, 1008, 1025
Asp718I GGTACC 1 cut(s) 553
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 1085
AvaII GGWCC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 553
BanII GRGCYC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 41, 1012, 1020
BccI CCATC 5 cut(s) 266, 280, 653, 695, 917
BceAI ACGGC 2 cut(s) 32, 58
BciT130I CCWGG 1 cut(s) 979
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 259, 355
BfaI CTAG 3 cut(s) 258, 269, 594
BfuAI ACCTGC 1 cut(s) 541
BisI GCNGC 4 cut(s) 30, 174, 1009, 1026
BlpI GCTNAGC 1 cut(s) 894
BlsI GCNGC 4 cut(s) 31, 175, 1010, 1027
Bme1390I CCNGG 1 cut(s) 979
Bme18I GGWCC 1 cut(s) 151
BmeRI GACNNNNNGTC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 495, 555
BmrFI CCNGG 1 cut(s) 979
BmsI GCATC 3 cut(s) 54, 457, 643
BpmI CTGGAG 1 cut(s) 121
Bpu1102I GCTNAGC 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 1094
BsaAI YACGTR 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 355
BsaJI CCNNGG 4 cut(s) 69, 154, 174, 978
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 613, 643
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 180, 530, 1096
Bse1I ACTGG 1 cut(s) 631
Bse3DI GCAATG 2 cut(s) 722, 772
BseBI CCWGG 1 cut(s) 979
BseDI CCNNGG 4 cut(s) 69, 154, 174, 978
BseGI GGATG 3 cut(s) 286, 472, 1083
BseLI CCNNNNNNNGG 4 cut(s) 18, 180, 530, 1096
BseMI GCAATG 2 cut(s) 722, 772
BseMII CTCAG 1 cut(s) 885
BseNI ACTGG 1 cut(s) 631
BseRI GAGGAG 2 cut(s) 58, 320
BseXI GCAGC 3 cut(s) 41, 1012, 1020
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 3 cut(s) 93, 176, 972
BshFI GGCC 2 cut(s) 884, 963
BshNI GGYRCC 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 107
BslI CCNNNNNNNGG 4 cut(s) 18, 180, 530, 1096
BsmAI GTCTC 2 cut(s) 259, 355
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 884, 963
Bso31I GGTCTC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 1060
Bsp143I GATC 3 cut(s) 133, 533, 1099
Bsp1720I GCTNAGC 1 cut(s) 894
Bsp19I CCATGG 1 cut(s) 154
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 7 cut(s) 19, 129, 174, 176, 194, 854, 1087
BspANI GGCC 2 cut(s) 884, 963
BspCNI CTCAG 1 cut(s) 886
BspFNI CGCG 3 cut(s) 93, 176, 972
BspLI GGNNCC 2 cut(s) 495, 555
BspMI ACCTGC 1 cut(s) 541
BspPI GGATC 2 cut(s) 541, 1107
BspT107I GGYRCC 1 cut(s) 553
BspTNI GGTCTC 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 722, 772
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 4 cut(s) 69, 154, 174, 978
BssMI GATC 3 cut(s) 133, 533, 1099
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 979
Bst4CI ACNGT 3 cut(s) 514, 812, 838
Bst6I CTCTTC 2 cut(s) 456, 939
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 1060
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 487, 576
BstDEI CTNAG 1 cut(s) 894
BstDSI CCRYGG 2 cut(s) 154, 174
BstF5I GGATG 3 cut(s) 286, 472, 1083
BstFNI CGCG 3 cut(s) 93, 176, 972
BstKTI GATC 3 cut(s) 136, 536, 1102
BstMAI GTCTC 2 cut(s) 259, 355
BstMBI GATC 3 cut(s) 133, 533, 1099
BstMWI GCNNNNNNNGC 5 cut(s) 392, 673, 890, 904, 969
BstNI CCWGG 1 cut(s) 979
BstSCI CCNGG 1 cut(s) 977
BstSNI TACGTA 1 cut(s) 344
BstUI CGCG 3 cut(s) 93, 176, 972
BstV1I GCAGC 3 cut(s) 41, 1012, 1020
BsuRI GGCC 2 cut(s) 884, 963
BtgI CCRYGG 2 cut(s) 154, 174
BtsCI GGATG 3 cut(s) 286, 472, 1083
BtsI GCAGTG 3 cut(s) 671, 874, 905
BtsIMutI CAGTG 4 cut(s) 671, 817, 874, 905
BtuMI TCGCGA 1 cut(s) 93
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 2 cut(s) 487, 576
Cfr13I GGNCC 1 cut(s) 151
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 1 cut(s) 899
Csp6I GTAC 3 cut(s) 477, 554, 1061
CviAII CATG 5 cut(s) 148, 155, 685, 715, 795
CviQI GTAC 3 cut(s) 477, 554, 1061
DdeI CTNAG 1 cut(s) 894
DpnI GATC 3 cut(s) 135, 535, 1101
DpnII GATC 3 cut(s) 133, 533, 1099
DrdI GACNNNNNNGTC 1 cut(s) 776
DriI GACNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 776
Eam1104I CTCTTC 2 cut(s) 456, 939
Eam1105I GACNNNNNGTC 1 cut(s) 368
EarI CTCTTC 2 cut(s) 456, 939
EciI GGCGGA 2 cut(s) 209, 1076
Ecl136II GAGCTC 1 cut(s) 105
Eco105I TACGTA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 154
Eco147I AGGCCT 2 cut(s) 884, 963
Eco24I GRGCYC 1 cut(s) 107
Eco31I GGTCTC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 897
EcoICRI GAGCTC 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 977
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 5 cut(s) 151, 158, 688, 718, 798
FatI CATG 5 cut(s) 147, 154, 684, 714, 794
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 30, 174, 1009, 1026
FokI GGATG 3 cut(s) 293, 479, 1070
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 4 cut(s) 30, 174, 1009, 1026
FspBI CTAG 3 cut(s) 258, 269, 594
GluI GCNGC 4 cut(s) 30, 174, 1009, 1026
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 2 cut(s) 884, 963
HgaI GACGC 1 cut(s) 899
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 5 cut(s) 151, 158, 688, 718, 798
HincII GTYRAC 2 cut(s) 264, 841
HindII GTYRAC 2 cut(s) 264, 841
HinfI GANTC 8 cut(s) 60, 74, 88, 113, 119, 265, 322, 695
HphI GGTGA 1 cut(s) 1085
Hpy166II GTNNAC 5 cut(s) 245, 264, 815, 841, 1061
Hpy188I TCNGA 5 cut(s) 73, 118, 374, 700, 934
Hpy188III TCNNGA 4 cut(s) 92, 100, 218, 269
Hpy8I GTNNAC 5 cut(s) 245, 264, 815, 841, 1061
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 103, 735, 1064
HpyCH4III ACNGT 3 cut(s) 514, 812, 838
HpyCH4IV ACGT 3 cut(s) 96, 343, 805
HpyCH4V TGCA 5 cut(s) 395, 470, 599, 722, 879
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 673, 890, 904, 969
HpyF3I CTNAG 1 cut(s) 894
HpySE526I ACGT 3 cut(s) 96, 343, 805
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 5 cut(s) 151, 158, 688, 718, 798
KpnI GGTACC 1 cut(s) 557
Ksp22I TGATCA 1 cut(s) 133
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 3 cut(s) 133, 533, 1099
LmnI GCTCC 6 cut(s) 71, 102, 196, 292, 436, 493
Lsp1109I GCAGC 3 cut(s) 41, 1012, 1020
LweI GCATC 3 cut(s) 54, 457, 643
MaeI CTAG 3 cut(s) 258, 269, 594
MaeII ACGT 3 cut(s) 96, 343, 805
MaeIII GTNAC 3 cut(s) 425, 542, 806
MalI GATC 3 cut(s) 135, 535, 1101
MboI GATC 3 cut(s) 133, 533, 1099
MboII GAAGA 7 cut(s) 114, 473, 704, 881, 932, 956, 1088
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 3 cut(s) 159, 200, 396
MlyI GAGTC 5 cut(s) 69, 83, 122, 259, 316
MmeI TCCRAC 2 cut(s) 96, 352
MseI TTAA 4 cut(s) 207, 645, 678, 735
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 2 cut(s) 131, 176
MspR9I CCNGG 1 cut(s) 979
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 1 cut(s) 979
MvnI CGCG 3 cut(s) 93, 176, 972
MwoI GCNNNNNNNGC 5 cut(s) 392, 673, 890, 904, 969
NcoI CCATGG 1 cut(s) 154
NdeII GATC 3 cut(s) 133, 533, 1099
NlaIII CATG 5 cut(s) 151, 158, 688, 718, 798
NlaIV GGNNCC 2 cut(s) 495, 555
NmuCI GTSAC 1 cut(s) 425
NruI TCGCGA 1 cut(s) 93
PceI AGGCCT 2 cut(s) 884, 963
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 3 cut(s) 88, 119, 695
PflMI CCANNNNNTGG 1 cut(s) 1096
PkrI GCNGC 4 cut(s) 31, 175, 1010, 1027
PleI GAGTC 5 cut(s) 68, 82, 121, 259, 316
PpsI GAGTC 5 cut(s) 68, 82, 121, 259, 316
Ppu21I YACGTR 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 977
PspGI CCWGG 1 cut(s) 977
PspN4I GGNNCC 2 cut(s) 495, 555
PspPI GGNCC 1 cut(s) 151
RruI TCGCGA 1 cut(s) 93
RsaI GTAC 3 cut(s) 478, 555, 1062
RsaNI GTAC 3 cut(s) 477, 554, 1061
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 107
SacII CCGCGG 1 cut(s) 177
SaqAI TTAA 4 cut(s) 207, 645, 678, 735
SatI GCNGC 4 cut(s) 30, 174, 1009, 1026
Sau3AI GATC 3 cut(s) 133, 533, 1099
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 5 cut(s) 69, 83, 122, 259, 316
ScrFI CCNGG 1 cut(s) 979
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 3 cut(s) 54, 457, 643
Sfr303I CCGCGG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
SnaBI TACGTA 1 cut(s) 344
Sse9I AATT 3 cut(s) 159, 200, 396
SseBI AGGCCT 2 cut(s) 884, 963
SsiI CCGC 7 cut(s) 19, 129, 174, 176, 194, 854, 1087
SspMI CTAG 3 cut(s) 258, 269, 594
SstI GAGCTC 1 cut(s) 107
StuI AGGCCT 2 cut(s) 884, 963
StyD4I CCNGG 1 cut(s) 977
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 514, 812, 838
TaiI ACGT 3 cut(s) 99, 346, 808
TaqI TCGA 2 cut(s) 107, 315
TasI AATT 3 cut(s) 159, 200, 396
TatI WGTACW 2 cut(s) 476, 1060
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 3 cut(s) 88, 119, 695
Tru1I TTAA 4 cut(s) 207, 645, 678, 735
Tru9I TTAA 4 cut(s) 207, 645, 678, 735
TscAI CASTG 4 cut(s) 678, 817, 881, 905
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 3 cut(s) 29, 1008, 1025
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 6 cut(s) 224, 432, 515, 596, 703, 882
TspGWI ACGGA 3 cut(s) 72, 262, 449
TspRI CASTG 4 cut(s) 678, 817, 881, 905
Van91I CCANNNNNTGG 1 cut(s) 1096
VpaK11BI GGWCC 1 cut(s) 151
XbaI TCTAGA 1 cut(s) 268
XspI CTAG 3 cut(s) 258, 269, 594
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.