MD07G1249100.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015180
Physical Location & Seq
Reverse (-)
31804054 .. 31807090
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1249100.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGCCCGTGGCCGAACGGCGGTCAGCTGCAGCCATGGAAAACGGCGTCGTAGAGAACGGTGTCTGCTCGTCGGAGTCGGCTACCGGGAGTCGCTACGTTTGGAGCTCCAAGGAGTCCGAATATTCCTCCGCTGATCATCTCGTTGTCATGGTCCATGGAATCATGGGAACTGCCGCGGATTGGAAGTTTGGGGCGGAGCAATTTGTTAAAACACTTCCAGACAAAGTTATTGTTCATTGTAGTGAACGGAATGGCTCTAGGCTGACTCTAGATGGTGTGGATGTAATGGGGGAGCGATTGGCAGAGGAGGTTATCGAACTGACTCGAAAAAAACCTAATCTACGAAAGATCTCATTTATTGGACATTCTGTTGGAGGATTAGTGGCAAGATATGCAATTGGGAGGCTATATAGACCCGGTCCCCCTAAAAGTGACAATACGGAACCTAAAAGTGAGAATACGGAACATTCATCTCCTGATGGATGTGAAGAGGATGCAAGGAGTACATTAGCTGGCTTGGAGCCTTTGAACTTTATTACTGTTGCCACGCCTCATCTTGGATCAAGGGGTAACAAGCAGGTGCCATTTCTCTTTGGTGTACCTGCCTTTGAAAGACTTGCTAGTGCGGTTATTCATTTGATATTTAGGAGAACAGGTCGGCATCTTTTTCTTAATGATGACGATGATGGGAAGCCTCCACTGCTTAAACGAATGATAGAAGATTGTGATGGTTGTTATTTCATGTCTGCATTGCGTAGTTTTAAGCGGAGGGTGGTGTATTCAAACGTGGGCTATGACCATATTGTCGGCTGGAAAACATCATGTATTAGACGTAATAGCGAACTTCCAAAGTGGGAAAATACTGTTGACGAAAAATATCCGCATATTGTTTATGAAGAACACTGCAAGGCCTATGATGCTGAGCAGTGTGAGCCTGCTTCAGCAGAAGATGGTGGCTCTGGCGAGCTTGAAGAGGAACTTTTGAAAGGCCTATCTCGCCTGTCTTGGGAAAAAGTAGATGTTAGTTTCCACAGCAGCAGACGGAGATTTTCTGCTCATAGTGTCATTCAGGTCAAAGATCAAAGTGTACATATAGAAGGTGAAGATGTCATACGCCATATAATGGATCATTTCCTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

381

Amino Acids

42.56

Weight (kDa)

6.08

Isoelectric Point (pI)

55.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 43 - 273 1.5e-64 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51180 AT5G51180
fragaria_vesca FvH4_7g26960
malus_domestica MD01G1177400.v1.1 MD07G1249100.v1.1
prunus_persica Prupe.2G272700_v2.0.a1
pyrus_communis pycom01g18790 pycom07g22230
rosa_chinensis RchiOBHm_Chr1g0373671
rosa_laevigata RLG00000026808
rosa_roxburghii Rroxscaffold_4G00284070
rosa_rugosa Rorug01G0378900
rosa_samantha Rh1AG388300 Rh1CG365600 Rh1DG383100
rosa_wichuraiana Rw1G034690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 568
AasI GACNNNNNNGTC 1 cut(s) 803
Acc36I ACCTGC 2 cut(s) 568, 610
AccB1I GGYRCC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 1123
AccII CGCG 1 cut(s) 176
AciI CCGC 8 cut(s) 19, 129, 174, 176, 194, 626, 766, 881
AclWI GGATC 2 cut(s) 568, 1134
AcoI YGGCCR 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 924
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 505, 600, 1089
AfiI CCNNNNNNNGG 5 cut(s) 18, 180, 557, 1006, 1123
AflII CTTAAG 1 cut(s) 1136
AgsI TTSAA 5 cut(s) 529, 611, 783, 971, 985
AluBI AGCT 4 cut(s) 26, 105, 512, 967
AluI AGCT 4 cut(s) 26, 105, 512, 967
Alw21I GWGCWC 1 cut(s) 107
AlwI GGATC 2 cut(s) 568, 1134
AoxI GGCC 3 cut(s) 9, 909, 988
ApeKI GCWGC 3 cut(s) 26, 29, 1035
AspS9I GGNCC 2 cut(s) 151, 419
AsuC2I CCSGG 2 cut(s) 85, 417
AsuHPI GGTGA 1 cut(s) 1112
AvaII GGWCC 2 cut(s) 151, 419
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 1095, 1127
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 13, 41, 1047
BccI CCATC 5 cut(s) 266, 473, 680, 722, 944
BceAI ACGGC 2 cut(s) 32, 58
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 2 cut(s) 85, 417
BfaI CTAG 3 cut(s) 258, 269, 621
BfmI CTRYAG 1 cut(s) 27
BfrI CTTAAG 1 cut(s) 1136
BfuAI ACCTGC 2 cut(s) 568, 610
BglII AGATCT 1 cut(s) 348
BisI GCNGC 4 cut(s) 27, 30, 174, 1036
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 4 cut(s) 28, 31, 175, 1037
Bme1390I CCNGG 2 cut(s) 85, 417
Bme18I GGWCC 2 cut(s) 151, 419
BmgT120I GGNCC 2 cut(s) 151, 419
BmiI GGNNCC 4 cut(s) 421, 444, 522, 582
BmrFI CCNGG 2 cut(s) 85, 417
BmsI GCATC 3 cut(s) 484, 670, 907
Bpu1102I GCTNAGC 1 cut(s) 921
BpuMI CCSGG 2 cut(s) 85, 417
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 5 cut(s) 6, 33, 108, 154, 174
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 640, 670
Bsc4I CCNNNNNNNGG 5 cut(s) 18, 180, 557, 1006, 1123
Bse3DI GCAATG 1 cut(s) 749
BseDI CCNNGG 5 cut(s) 6, 33, 108, 154, 174
BseGI GGATG 3 cut(s) 286, 488, 499
BseLI CCNNNNNNNGG 5 cut(s) 18, 180, 557, 1006, 1123
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 1 cut(s) 912
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 3 cut(s) 13, 41, 1047
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 3 cut(s) 11, 911, 990
BshNI GGYRCC 1 cut(s) 580
BsiHKAI GWGCWC 1 cut(s) 107
BsiSI CCGG 2 cut(s) 84, 417
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 5 cut(s) 18, 180, 557, 1006, 1123
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 3 cut(s) 11, 911, 990
Bsp1286I GDGCHC 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 1087
Bsp143I GATC 5 cut(s) 133, 348, 560, 1078, 1126
Bsp1720I GCTNAGC 1 cut(s) 921
Bsp19I CCATGG 2 cut(s) 33, 154
BspACI CCGC 8 cut(s) 19, 129, 174, 176, 194, 626, 766, 881
BspANI GGCC 3 cut(s) 11, 911, 990
BspCNI CTCAG 1 cut(s) 913
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 4 cut(s) 421, 444, 522, 582
BspMAI CTGCAG 1 cut(s) 31
BspMI ACCTGC 2 cut(s) 568, 610
BspPI GGATC 2 cut(s) 568, 1134
BspT107I GGYRCC 1 cut(s) 580
BspTI CTTAAG 1 cut(s) 1136
BsrDI GCAATG 1 cut(s) 749
BsrGI TGTACA 1 cut(s) 1087
BssECI CCNNGG 5 cut(s) 6, 33, 108, 154, 174
BssMI GATC 5 cut(s) 133, 348, 560, 1078, 1126
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 3 cut(s) 33, 108, 154
Bst4CI ACNGT 3 cut(s) 59, 541, 865
Bst6I CTCTTC 2 cut(s) 483, 966
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 1136
BstAPI GCANNNNNTGC 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 1087
BstC8I GCNNGC 3 cut(s) 514, 936, 965
BstDEI CTNAG 1 cut(s) 921
BstDSI CCRYGG 4 cut(s) 6, 33, 154, 174
BstF5I GGATG 3 cut(s) 286, 488, 499
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 5 cut(s) 136, 351, 563, 1081, 1129
BstMBI GATC 5 cut(s) 133, 348, 560, 1078, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 392, 700, 917, 931, 996
BstSCI CCNGG 2 cut(s) 83, 415
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 13, 41, 1047
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 3 cut(s) 11, 911, 990
BtgI CCRYGG 4 cut(s) 6, 33, 154, 174
BtsCI GGATG 3 cut(s) 286, 488, 499
BtsI GCAGTG 3 cut(s) 698, 901, 932
BtsIMutI CAGTG 3 cut(s) 698, 901, 932
BveI ACCTGC 2 cut(s) 568, 610
Cac8I GCNNGC 3 cut(s) 514, 936, 965
Cfr13I GGNCC 2 cut(s) 151, 419
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 504, 599, 1088
CviAII CATG 6 cut(s) 34, 148, 155, 163, 742, 822
CviQI GTAC 3 cut(s) 504, 599, 1088
DdeI CTNAG 1 cut(s) 921
DpnI GATC 5 cut(s) 135, 350, 562, 1080, 1128
DpnII GATC 5 cut(s) 133, 348, 560, 1078, 1126
DrdI GACNNNNNNGTC 1 cut(s) 803
DseDI GACNNNNNNGTC 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 483, 966
EarI CTCTTC 2 cut(s) 483, 966
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 3 cut(s) 33, 108, 154
Eco147I AGGCCT 2 cut(s) 911, 990
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 151, 419
Eco53kI GAGCTC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 924
EcoICRI GAGCTC 1 cut(s) 105
EcoT14I CCWWGG 3 cut(s) 33, 108, 154
EcoT38I GRGCYC 1 cut(s) 107
ErhI CCWWGG 3 cut(s) 33, 108, 154
FaeI CATG 6 cut(s) 37, 151, 158, 166, 745, 825
FalI AAGNNNNNCTT 2 cut(s) 963, 995
FaqI GGGAC 1 cut(s) 405
FatI CATG 6 cut(s) 33, 147, 154, 162, 741, 821
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 27, 30, 174, 1036
FokI GGATG 3 cut(s) 293, 495, 506
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 4 cut(s) 27, 30, 174, 1036
FspBI CTAG 3 cut(s) 258, 269, 621
GluI GCNGC 4 cut(s) 27, 30, 174, 1036
HaeIII GGCC 3 cut(s) 11, 911, 990
HapII CCGG 2 cut(s) 84, 417
HgaI GACGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 6 cut(s) 37, 151, 158, 166, 745, 825
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 6 cut(s) 74, 88, 113, 159, 265, 322
HpaII CCGG 2 cut(s) 84, 417
HphI GGTGA 1 cut(s) 1112
Hpy166II GTNNAC 4 cut(s) 245, 599, 868, 1088
Hpy188I TCNGA 2 cut(s) 73, 118
Hpy188III TCNNGA 3 cut(s) 218, 269, 476
Hpy8I GTNNAC 4 cut(s) 245, 599, 868, 1088
Hpy99I CGWCG 2 cut(s) 50, 73
HpyAV CCTTC 1 cut(s) 1091
HpyCH4III ACNGT 3 cut(s) 59, 541, 865
HpyCH4IV ACGT 3 cut(s) 96, 786, 832
HpyCH4V TGCA 5 cut(s) 29, 395, 497, 749, 906
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 700, 917, 931, 996
HpyF3I CTNAG 1 cut(s) 921
HpySE526I ACGT 3 cut(s) 96, 786, 832
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 6 cut(s) 37, 151, 158, 166, 745, 825
Ksp22I TGATCA 1 cut(s) 133
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 5 cut(s) 133, 348, 560, 1078, 1126
LmnI GCTCC 5 cut(s) 102, 110, 196, 292, 520
Lsp1109I GCAGC 3 cut(s) 13, 41, 1047
LweI GCATC 3 cut(s) 484, 670, 907
MaeI CTAG 3 cut(s) 258, 269, 621
MaeII ACGT 3 cut(s) 96, 786, 832
MaeIII GTNAC 2 cut(s) 431, 569
MalI GATC 5 cut(s) 135, 350, 562, 1080, 1128
MboI GATC 5 cut(s) 133, 348, 560, 1078, 1126
MboII GAAGA 6 cut(s) 500, 731, 908, 959, 983, 1115
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 2 cut(s) 200, 396
MlyI GAGTC 5 cut(s) 83, 97, 122, 259, 316
MmeI TCCRAC 2 cut(s) 51, 352
MseI TTAA 5 cut(s) 207, 672, 705, 762, 1137
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 3 cut(s) 26, 131, 176
MspCI CTTAAG 1 cut(s) 1136
MspI CCGG 2 cut(s) 84, 417
MspR9I CCNGG 2 cut(s) 85, 417
MunI CAATTG 1 cut(s) 396
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 5 cut(s) 392, 700, 917, 931, 996
NciI CCSGG 2 cut(s) 85, 417
NcoI CCATGG 2 cut(s) 33, 154
NdeII GATC 5 cut(s) 133, 348, 560, 1078, 1126
NlaIII CATG 6 cut(s) 37, 151, 158, 166, 745, 825
NlaIV GGNNCC 4 cut(s) 421, 444, 522, 582
NmuCI GTSAC 1 cut(s) 431
PaqCI CACCTGC 1 cut(s) 568
PceI AGGCCT 2 cut(s) 911, 990
PfeI GAWTC 1 cut(s) 159
PflFI GACNNNGTC 1 cut(s) 417
PflMI CCANNNNNTGG 1 cut(s) 1123
PkrI GCNGC 4 cut(s) 28, 31, 175, 1037
PleI GAGTC 5 cut(s) 82, 96, 121, 259, 316
PpsI GAGTC 5 cut(s) 82, 96, 121, 259, 316
Psp124BI GAGCTC 1 cut(s) 107
PspN4I GGNNCC 4 cut(s) 421, 444, 522, 582
PspPI GGNCC 2 cut(s) 151, 419
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 348
PsyI GACNNNGTC 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 3 cut(s) 505, 600, 1089
RsaNI GTAC 3 cut(s) 504, 599, 1088
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 107
SacII CCGCGG 1 cut(s) 177
SaqAI TTAA 5 cut(s) 207, 672, 705, 762, 1137
SatI GCNGC 4 cut(s) 27, 30, 174, 1036
Sau3AI GATC 5 cut(s) 133, 348, 560, 1078, 1126
Sau96I GGNCC 2 cut(s) 151, 419
SchI GAGTC 5 cut(s) 83, 97, 122, 259, 316
ScrFI CCNGG 2 cut(s) 85, 417
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 3 cut(s) 484, 670, 907
SfcI CTRYAG 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 151, 419
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
Sse9I AATT 2 cut(s) 200, 396
SseBI AGGCCT 2 cut(s) 911, 990
SsiI CCGC 8 cut(s) 19, 129, 174, 176, 194, 626, 766, 881
SspI AATATT 1 cut(s) 122
SspMI CTAG 3 cut(s) 258, 269, 621
SstI GAGCTC 1 cut(s) 107
StuI AGGCCT 2 cut(s) 911, 990
StyD4I CCNGG 2 cut(s) 83, 415
StyI CCWWGG 3 cut(s) 33, 108, 154
TaaI ACNGT 3 cut(s) 59, 541, 865
TaiI ACGT 3 cut(s) 99, 789, 835
TaqI TCGA 2 cut(s) 315, 325
TasI AATT 2 cut(s) 200, 396
TatI WGTACW 2 cut(s) 503, 1087
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 5 cut(s) 207, 672, 705, 762, 1137
Tru9I TTAA 5 cut(s) 207, 672, 705, 762, 1137
TscAI CASTG 3 cut(s) 705, 908, 932
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 3 cut(s) 26, 29, 1035
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 5 cut(s) 224, 459, 623, 730, 909
TspGWI ACGGA 4 cut(s) 262, 455, 476, 1057
TspRI CASTG 3 cut(s) 705, 908, 932
Tth111I GACNNNGTC 1 cut(s) 417
Van91I CCANNNNNTGG 1 cut(s) 1123
Vha464I CTTAAG 1 cut(s) 1136
VpaK11BI GGWCC 2 cut(s) 151, 419
XbaI TCTAGA 1 cut(s) 268
XspI CTAG 3 cut(s) 258, 269, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.