RchiOBHm_Chr1g0373671

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015180
Physical Location & Seq
Reverse (-)
61777514 .. 61781007
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59753

Sequence Viewer

Length: 1086 bp
ATGGAAAACGGCGTCGTCAAGGAGAACGGAGTTTGCTCGTCGGAGTCGGTTAATGGCAGTCGCGACGTCTGGAGTGCTAAGGAATCCGATTCTTCATCCGCTGACCATCTCGTCGTCATGGTCCATGGGATTATGGGAAGTACCTCAGATTGGAAGTTTGGAGCAGAGCAGTTTGTTAAATTGCTCCCCGACAAAGTTCTTGTTCATTGTAGTGAACGGAATACCTCTACGCTGACTCTGGATGGTGTGGATGTAATGGGAGAGCGCTTGGCAGGAGAGGTTGTCGAACTGGTTCAAAGAAAGCCAAATTTACGTAAGATCTCATTCGTTGCACATTCTGTTGGAGGGTTAGTGGCAAGATATGCAATTGGGAGACTATATAGACCTGCTAAGGGAGAAAATATGGAAAATTCACATCCTAATGGCTGTGAAGAGGATTCAAGGAGTACAATATGTGGCTTGGAGCCTATGAATTTCATCACTGTTGCTACACCTCATCTTGGTTCAAGGGGTAACAAGCAGGTGCCATTTCTTTTTGGCGTACCTGCCTTCGAAAAACTTGCTAGTGCAGTTATCCATTTGATATTTAGGAGAACAGGTAGGCATCTGTTTCTTAATGACGATGACGATGGCAAGCCTCCACTGCTTAAACGAATGATAGAAGATTATGATGGCTGTTATTTCATGTCGGCATTGCGTAGTTTCAGACGTAGGGTGGCATATTCAAACGTGGGCTATGACCATATTGTCGGCTGGAGAACATCATGCATTAGACGTACTTGTGAACTGCCGAAGTGGGAGGATACTGTTGATGAAAAATATCCACATATAGTTTATGAAGAGCACTGCAAGGCCTATGATGCTGAGCAGTGTGAGCCCACTTCAGCAGAAGGCAATGACTGTGACCAGCTTGAAGGGGAGCTAGTGACGGGCCTATCTCGTGTGTCATGGGAAAAAGTAGATGTTAGTTTCCACTGCAGCAAACAGAGATTTGCTGCTCATAGTGTCATTCAGGTAAAAGATCAAACTGCACATAAAGAAGGCGAAGATGTTATACGTCATATGATTGATCATTTCCTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

361

Amino Acids

40.57

Weight (kDa)

5.94

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 32 - 254 5.9e-68 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51180 AT5G51180
fragaria_vesca FvH4_7g26960
malus_domestica MD01G1177400.v1.1 MD07G1249100.v1.1
prunus_persica Prupe.2G272700_v2.0.a1
pyrus_communis pycom01g18790 pycom07g22230
rosa_chinensis RchiOBHm_Chr1g0373671
rosa_laevigata RLG00000026808
rosa_roxburghii Rroxscaffold_4G00284070
rosa_rugosa Rorug01G0378900
rosa_samantha Rh1AG388300 Rh1CG365600 Rh1DG383100
rosa_wichuraiana Rw1G034690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
AasI GACNNNNNNGTC 2 cut(s) 110, 746
AatII GACGTC 1 cut(s) 69
Acc36I ACCTGC 3 cut(s) 394, 511, 553
AccB1I GGYRCC 1 cut(s) 523
AccII CGCG 1 cut(s) 63
AciI CCGC 1 cut(s) 99
AcsI RAATTY 3 cut(s) 307, 409, 472
AcuI CTGAAG 1 cut(s) 867
AcyI GRCGYC 2 cut(s) 12, 66
AfaI GTAC 4 cut(s) 142, 448, 543, 778
AfeI AGCGCT 1 cut(s) 266
AfiI CCNNNNNNNGG 3 cut(s) 150, 392, 500
AgsI TTSAA 5 cut(s) 296, 441, 507, 726, 914
AluBI AGCT 2 cut(s) 910, 922
AluI AGCT 2 cut(s) 910, 922
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 1 cut(s) 367
Aor51HI AGCGCT 1 cut(s) 266
AoxI GGCC 2 cut(s) 852, 931
ApeKI GCWGC 2 cut(s) 978, 995
ApoI RAATTY 3 cut(s) 307, 409, 472
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 121, 931
AsuII TTCGAA 1 cut(s) 552
AvaII GGWCC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 1 cut(s) 879
BarI GAAGNNNNNNTAC 2 cut(s) 1038, 1070
BauI CACGAG 1 cut(s) 939
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 2 cut(s) 982, 990
BccI CCATC 4 cut(s) 114, 236, 623, 665
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciVI GTATCC 1 cut(s) 796
BclI TGATCA 1 cut(s) 1069
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 2 cut(s) 564, 923
BfmI CTRYAG 1 cut(s) 976
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 3 cut(s) 394, 511, 553
BfuI GTATCC 1 cut(s) 796
BglII AGATCT 1 cut(s) 318
BisI GCNGC 2 cut(s) 979, 996
BlpI GCTNAGC 1 cut(s) 864
BlsI GCNGC 2 cut(s) 980, 997
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 2 cut(s) 121, 931
BmiI GGNNCC 2 cut(s) 465, 525
BmsI GCATC 2 cut(s) 613, 850
BpmI CTGGAG 2 cut(s) 91, 775
Bpu10I CCTNAGC 2 cut(s) 78, 390
Bpu1102I GCTNAGC 1 cut(s) 864
Bpu14I TTCGAA 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 314
BsaHI GRCGYC 2 cut(s) 12, 66
BsaJI CCNNGG 1 cut(s) 124
BsaXI ACNNNNNCTCC 6 cut(s) 267, 297, 336, 366, 583, 613
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 392, 500
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 2 cut(s) 692, 901
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 4 cut(s) 95, 247, 256, 415
BseLI CCNNNNNNNGG 3 cut(s) 150, 392, 500
BseMI GCAATG 2 cut(s) 692, 901
BseMII CTCAG 2 cut(s) 159, 855
BseNI ACTGG 1 cut(s) 294
BseXI GCAGC 2 cut(s) 982, 990
BsgI GTGCAG 2 cut(s) 588, 1014
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 854, 933
BshNI GGYRCC 1 cut(s) 523
BsiHKAI GWGCWC 1 cut(s) 846
BslI CCNNNNNNNGG 3 cut(s) 150, 392, 500
BsmAI GTCTC 1 cut(s) 367
BsnI GGCC 2 cut(s) 854, 933
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 846, 879
Bsp143I GATC 3 cut(s) 318, 1021, 1069
Bsp1720I GCTNAGC 1 cut(s) 864
Bsp19I CCATGG 1 cut(s) 124
Bsp68I TCGCGA 1 cut(s) 63
BspACI CCGC 1 cut(s) 99
BspANI GGCC 2 cut(s) 854, 933
BspCNI CTCAG 2 cut(s) 158, 856
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 465, 525
BspMAI CTGCAG 1 cut(s) 980
BspMI ACCTGC 3 cut(s) 394, 511, 553
BspQI GCTCTTC 1 cut(s) 834
BspT104I TTCGAA 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 523
BsrDI GCAATG 2 cut(s) 692, 901
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 3 cut(s) 318, 1021, 1069
BssNI GRCGYC 2 cut(s) 12, 66
BssSI CACGAG 1 cut(s) 939
BssT1I CCWWGG 1 cut(s) 124
Bst2BI CACGAG 1 cut(s) 939
Bst4CI ACNGT 3 cut(s) 484, 808, 902
Bst6I CTCTTC 2 cut(s) 426, 834
BstACI GRCGYC 2 cut(s) 12, 66
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 1 cut(s) 635
BstDEI CTNAG 4 cut(s) 78, 145, 390, 864
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 4 cut(s) 95, 247, 256, 415
BstFNI CGCG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 3 cut(s) 321, 1024, 1072
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 3 cut(s) 318, 1021, 1069
BstMWI GCNNNNNNNGC 4 cut(s) 362, 643, 860, 874
BstSFI CTRYAG 1 cut(s) 976
BstSNI TACGTA 1 cut(s) 314
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 2 cut(s) 982, 990
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BsuI GTATCC 1 cut(s) 796
BsuRI GGCC 2 cut(s) 854, 933
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 4 cut(s) 95, 247, 256, 415
BtsI GCAGTG 4 cut(s) 641, 844, 875, 973
BtsIMutI CAGTG 5 cut(s) 480, 641, 844, 875, 973
BtuMI TCGCGA 1 cut(s) 63
BveI ACCTGC 3 cut(s) 394, 511, 553
Cac8I GCNNGC 1 cut(s) 635
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 2 cut(s) 121, 931
Csp6I GTAC 4 cut(s) 141, 447, 542, 777
CviAII CATG 5 cut(s) 118, 125, 685, 765, 948
CviQI GTAC 4 cut(s) 141, 447, 542, 777
DdeI CTNAG 4 cut(s) 78, 145, 390, 864
DpnI GATC 3 cut(s) 320, 1023, 1071
DpnII GATC 3 cut(s) 318, 1021, 1069
DrdI GACNNNNNNGTC 2 cut(s) 110, 746
DseDI GACNNNNNNGTC 2 cut(s) 110, 746
Eam1104I CTCTTC 2 cut(s) 426, 834
EarI CTCTTC 2 cut(s) 426, 834
Eco105I TACGTA 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 124
Eco147I AGGCCT 1 cut(s) 854
Eco24I GRGCYC 1 cut(s) 879
Eco47I GGWCC 1 cut(s) 121
Eco47III AGCGCT 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 867
EcoT14I CCWWGG 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 770
EcoT38I GRGCYC 1 cut(s) 879
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 5 cut(s) 121, 128, 688, 768, 951
FatI CATG 5 cut(s) 117, 124, 684, 764, 947
FauNDI CATATG 1 cut(s) 1062
FbaI TGATCA 1 cut(s) 1069
Fnu4HI GCNGC 2 cut(s) 979, 996
FokI GGATG 4 cut(s) 82, 254, 263, 402
FriOI GRGCYC 1 cut(s) 879
Fsp4HI GCNGC 2 cut(s) 979, 996
FspBI CTAG 2 cut(s) 564, 923
GlaI GCGC 1 cut(s) 266
GluI GCNGC 2 cut(s) 979, 996
GsuI CTGGAG 2 cut(s) 91, 775
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 2 cut(s) 854, 933
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 2 cut(s) 12, 66
Hin1II CATG 5 cut(s) 121, 128, 688, 768, 951
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 5 cut(s) 44, 83, 89, 235, 437
Hpy166II GTNNAC 2 cut(s) 215, 785
Hpy188I TCNGA 4 cut(s) 43, 88, 148, 707
Hpy188III TCNNGA 3 cut(s) 62, 70, 239
Hpy8I GTNNAC 2 cut(s) 215, 785
Hpy99I CGWCG 4 cut(s) 17, 43, 68, 116
HpyAV CCTTC 4 cut(s) 559, 884, 908, 1034
HpyCH4III ACNGT 3 cut(s) 484, 808, 902
HpyCH4IV ACGT 6 cut(s) 66, 313, 709, 729, 775, 1057
HpyCH4V TGCA 7 cut(s) 332, 365, 569, 768, 849, 978, 1031
HpyF10VI GCNNNNNNNGC 4 cut(s) 362, 643, 860, 874
HpyF3I CTNAG 4 cut(s) 78, 145, 390, 864
HpySE526I ACGT 6 cut(s) 66, 313, 709, 729, 775, 1057
Hsp92I GRCGYC 2 cut(s) 12, 66
Hsp92II CATG 5 cut(s) 121, 128, 688, 768, 951
HspAI GCGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 1069
Kzo9I GATC 3 cut(s) 318, 1021, 1069
LguI GCTCTTC 1 cut(s) 834
LmnI GCTCC 4 cut(s) 161, 189, 463, 919
Lsp1109I GCAGC 2 cut(s) 982, 990
LweI GCATC 2 cut(s) 613, 850
MaeI CTAG 2 cut(s) 564, 923
MaeII ACGT 6 cut(s) 66, 313, 709, 729, 775, 1057
MaeIII GTNAC 3 cut(s) 512, 902, 925
MalI GATC 3 cut(s) 320, 1023, 1071
MboI GATC 3 cut(s) 318, 1021, 1069
MboII GAAGA 5 cut(s) 84, 443, 674, 851, 1058
MfeI CAATTG 1 cut(s) 366
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 846, 879
MluCI AATT 5 cut(s) 179, 307, 366, 409, 472
MlyI GAGTC 2 cut(s) 53, 229
MmeI TCCRAC 2 cut(s) 21, 322
MnlI CCTC 8 cut(s) 154, 235, 271, 338, 427, 504, 648, 793
Mph1103I ATGCAT 1 cut(s) 770
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 5 cut(s) 51, 177, 615, 648, 1084
MslI CAYNNNNRTG 2 cut(s) 210, 420
MspA1I CMGCKG 1 cut(s) 101
MunI CAATTG 1 cut(s) 366
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 4 cut(s) 362, 643, 860, 874
NcoI CCATGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 1062
NdeII GATC 3 cut(s) 318, 1021, 1069
NlaIII CATG 5 cut(s) 121, 128, 688, 768, 951
NlaIV GGNNCC 2 cut(s) 465, 525
NmuCI GTSAC 2 cut(s) 902, 925
NruI TCGCGA 1 cut(s) 63
NsiI ATGCAT 1 cut(s) 770
NspV TTCGAA 1 cut(s) 552
PaqCI CACCTGC 1 cut(s) 511
PceI AGGCCT 1 cut(s) 854
PciSI GCTCTTC 1 cut(s) 834
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 83, 89, 437
PkrI GCNGC 2 cut(s) 980, 997
PleI GAGTC 2 cut(s) 52, 229
PpsI GAGTC 2 cut(s) 52, 229
Ppu21I YACGTR 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 465, 525
PspPI GGNCC 2 cut(s) 121, 931
PstI CTGCAG 1 cut(s) 980
PsuI RGATCY 1 cut(s) 318
RruI TCGCGA 1 cut(s) 63
RsaI GTAC 4 cut(s) 142, 448, 543, 778
RsaNI GTAC 4 cut(s) 141, 447, 542, 777
RseI CAYNNNNRTG 2 cut(s) 210, 420
SapI GCTCTTC 1 cut(s) 834
SaqAI TTAA 5 cut(s) 51, 177, 615, 648, 1084
SatI GCNGC 2 cut(s) 979, 996
Sau3AI GATC 3 cut(s) 318, 1021, 1069
Sau96I GGNCC 2 cut(s) 121, 931
SchI GAGTC 2 cut(s) 53, 229
SduI GDGCHC 2 cut(s) 846, 879
SfaNI GCATC 2 cut(s) 613, 850
SfcI CTRYAG 1 cut(s) 976
SfuI TTCGAA 1 cut(s) 552
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 210, 420
SnaBI TACGTA 1 cut(s) 314
Sse9I AATT 5 cut(s) 179, 307, 366, 409, 472
SseBI AGGCCT 1 cut(s) 854
SsiI CCGC 1 cut(s) 99
SspMI CTAG 2 cut(s) 564, 923
StuI AGGCCT 1 cut(s) 854
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 3 cut(s) 484, 808, 902
TaiI ACGT 6 cut(s) 69, 316, 712, 732, 778, 1060
TaqI TCGA 2 cut(s) 285, 552
TasI AATT 5 cut(s) 179, 307, 366, 409, 472
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 3 cut(s) 83, 89, 437
Tru1I TTAA 5 cut(s) 51, 177, 615, 648, 1084
Tru9I TTAA 5 cut(s) 51, 177, 615, 648, 1084
TscAI CASTG 5 cut(s) 487, 648, 851, 875, 980
TseFI GTSAC 2 cut(s) 902, 925
TseI GCWGC 2 cut(s) 978, 995
Tsp45I GTSAC 2 cut(s) 902, 925
TspDTI ATGAA 7 cut(s) 84, 194, 466, 485, 673, 828, 852
TspGWI ACGGA 2 cut(s) 42, 232
TspRI CASTG 5 cut(s) 487, 648, 851, 875, 980
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 3 cut(s) 307, 409, 472
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 2 cut(s) 564, 923
ZraI GACGTC 1 cut(s) 67
Zsp2I ATGCAT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.