FvH4_7g31620

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008217
Physical Location & Seq
Forward (+)
22819542 .. 22823544
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31620.t1

Sequence Viewer

Length: 639 bp
ATGGGCTGCTATTGTTCAAAGCCAGAGAAAACTCCGGGCTATGAGGACCCTGTAGTTCTTGCTGCTGAAACACCTTTTACAGTGAGTGAAGTAGAAGCCTTGTATGAGCTTTTCAAGAAATTGAGCAGTTCTATATATGATGATGGGCTCATCCACAAGGAAGAATTTCAACTAGCGCTCTTCAGGAACCAAAATAAGAAGAATCTTTTTGCAGATAGGATTTTCGACTTGTTTGATGTCAAGCGCAATGGGGTTATTGATTTTGGAGAATTTGTTCGATCATTGGGTTTCTTTCACCCCAATGCACCTGTAGAAGACAAAATTGCATTTGCGTTTAGACTGTATGATCTGAGACAAACAGGATTCATTGAACGAGAGGAGTTGAAGGAGATGGTGTTGGCACTTTTGCATGAATCAGATTTGTTACTTTCAGATGATGTAGTCGAAGCCATTGTGGATAAGACATTTAGTGATGCAGATAAAAAAGGAGATGGGAGGATTGATCAGCAAGAGTGGAAAGAGTATGTTTTGAAGCATCCAACTTTGATCAAGAATATGACTCTACCATATCTAAAGGACATAACACTAGCTTTCCCTAGCTTTGTAATGAGTTCTGAAGTTGAAGATTATGAAATGTGA

Protein Analysis

213

Amino Acids

24.5

Weight (kDa)

4.69

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 105 - 175 5e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 164, 269
AcuI CTGAAG 2 cut(s) 166, 636
AfeI AGCGCT 1 cut(s) 177
AgsI TTSAA 7 cut(s) 18, 115, 170, 371, 385, 532, 623
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 109, 590, 600
AluI AGCT 3 cut(s) 109, 590, 600
Alw26I GTCTC 1 cut(s) 346
Aor51HI AGCGCT 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 6, 62
ApoI RAATTY 2 cut(s) 164, 269
Asp700I GAANNNNTTC 2 cut(s) 165, 273
AspLEI GCGC 2 cut(s) 178, 246
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 49
BccI CCATC 3 cut(s) 137, 385, 485
BclI TGATCA 2 cut(s) 502, 546
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 3 cut(s) 173, 587, 597
BfmI CTRYAG 2 cut(s) 51, 309
BfoI RGCGCY 1 cut(s) 179
BisI GCNGC 2 cut(s) 7, 63
BlsI GCNGC 2 cut(s) 8, 64
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 48, 188
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 2 cut(s) 463, 544
BpiI GAAGAC 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 36
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 380, 410
Bse3DI GCAATG 1 cut(s) 253
BseGI GGATG 2 cut(s) 150, 535
BseMI GCAATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 278, 346, 502, 546
BspCNI CTCAG 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 48, 188
BspQI GCTCTTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 253
BssMI GATC 4 cut(s) 278, 346, 502, 546
Bst4CI ACNGT 2 cut(s) 82, 342
Bst6I CTCTTC 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 150, 535
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 2 cut(s) 178, 246
BstKTI GATC 4 cut(s) 281, 349, 505, 549
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 4 cut(s) 278, 346, 502, 546
BstSCI CCNGG 1 cut(s) 34
BstSFI CTRYAG 2 cut(s) 51, 309
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 321
BtsCI GGATG 2 cut(s) 150, 535
BtsIMutI CAGTG 1 cut(s) 87
CfoI GCGC 2 cut(s) 178, 246
Cfr13I GGNCC 1 cut(s) 46
CviAII CATG 1 cut(s) 410
CviJI RGCY 9 cut(s) 6, 22, 39, 98, 109, 148, 449, 590, 600
CviKI_1 RGCY 9 cut(s) 6, 22, 39, 98, 109, 148, 449, 590, 600
DdeI CTNAG 1 cut(s) 350
DpnI GATC 4 cut(s) 280, 348, 504, 548
DpnII GATC 4 cut(s) 278, 346, 502, 546
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 166, 636
EcoO109I RGGNCCY 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 1 cut(s) 413
FatI CATG 1 cut(s) 409
FbaI TGATCA 2 cut(s) 502, 546
Fnu4HI GCNGC 2 cut(s) 7, 63
FokI GGATG 2 cut(s) 137, 522
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 7, 63
FspBI CTAG 3 cut(s) 173, 587, 597
GlaI GCGC 2 cut(s) 177, 245
GluI GCNGC 2 cut(s) 7, 63
HaeII RGCGCY 1 cut(s) 179
HapII CCGG 1 cut(s) 35
HhaI GCGC 2 cut(s) 178, 246
Hin1II CATG 1 cut(s) 413
Hin6I GCGC 2 cut(s) 176, 244
HinP1I GCGC 2 cut(s) 176, 244
HinfI GANTC 4 cut(s) 202, 363, 413, 559
HpaII CCGG 1 cut(s) 35
HphI GGTGA 1 cut(s) 287
Hpy188I TCNGA 4 cut(s) 351, 418, 433, 616
Hpy188III TCNNGA 3 cut(s) 115, 184, 550
HpyAV CCTTC 1 cut(s) 379
HpyCH4III ACNGT 2 cut(s) 82, 342
HpyCH4V TGCA 5 cut(s) 212, 305, 326, 409, 476
HpyF3I CTNAG 1 cut(s) 350
Hsp92II CATG 1 cut(s) 413
HspAI GCGC 2 cut(s) 176, 244
Ksp22I TGATCA 2 cut(s) 502, 546
Kzo9I GATC 4 cut(s) 278, 346, 502, 546
LguI GCTCTTC 1 cut(s) 185
LpnPI CCDG 6 cut(s) 36, 48, 63, 169, 321, 345
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 2 cut(s) 463, 544
MaeI CTAG 3 cut(s) 173, 587, 597
MaeIII GTNAC 1 cut(s) 423
MalI GATC 4 cut(s) 280, 348, 504, 548
MboI GATC 4 cut(s) 278, 346, 502, 546
MboII GAAGA 5 cut(s) 172, 173, 211, 326, 635
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 4 cut(s) 119, 164, 269, 321
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 3 cut(s) 37, 370, 489
MroXI GAANNNNTTC 2 cut(s) 165, 273
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 36
NciI CCSGG 1 cut(s) 36
NdeII GATC 4 cut(s) 278, 346, 502, 546
NlaIII CATG 1 cut(s) 413
NlaIV GGNNCC 2 cut(s) 48, 188
PciSI GCTCTTC 1 cut(s) 185
PdmI GAANNNNTTC 2 cut(s) 165, 273
PfeI GAWTC 3 cut(s) 202, 363, 413
PkrI GCNGC 2 cut(s) 8, 64
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PpuMI RGGWCCY 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 46
PspN4I GGNNCC 2 cut(s) 48, 188
PspPI GGNCC 1 cut(s) 46
PspPPI RGGWCCY 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 185
SatI GCNGC 2 cut(s) 7, 63
Sau3AI GATC 4 cut(s) 278, 346, 502, 546
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 150
SetI ASST 5 cut(s) 76, 111, 310, 592, 602
SfaNI GCATC 2 cut(s) 463, 544
SfcI CTRYAG 2 cut(s) 51, 309
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 4 cut(s) 119, 164, 269, 321
SspMI CTAG 3 cut(s) 173, 587, 597
StyD4I CCNGG 1 cut(s) 34
TaaI ACNGT 2 cut(s) 82, 342
TaqI TCGA 3 cut(s) 225, 277, 444
TasI AATT 4 cut(s) 119, 164, 269, 321
TfiI GAWTC 3 cut(s) 202, 363, 413
TscAI CASTG 1 cut(s) 87
TseI GCWGC 2 cut(s) 6, 62
TspDTI ATGAA 2 cut(s) 355, 426
TspRI CASTG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 2 cut(s) 164, 269
XmnI GAANNNNTTC 2 cut(s) 165, 273
XspI CTAG 3 cut(s) 173, 587, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.