MD07G1288800.v1.1

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008217
Physical Location & Seq
Forward (+)
34980079 .. 34983632
3554 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1288800.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGGCTGCTATTGTTCGAAGGAAGCTAAAACGCCTGGCTATGAGGACCCTGCGACTCTTGCTGCTGCAACACCTTTTACTGTGAATGAAGTAGAAGCCTTGTACGAGCTTTTTAAGAAATTGAGCAGTTCGATAATTGATGACGGACTTATCCACAAGGAAGAATTTCAGCTCGCGCTCTTCAGGAACAAAAATCAAAGGAATCTTTTTGCAGACAGGATTTTTGACTTATTTGATGTCAAGCGCAATGGGGTTATTGAGTTTGGAGAATTTGTTCGATCATTAGGTGTCTTTCACCCCGATGCACCTGTAGAAGACAAAATTTCATTTGCTTTTAGACTGTATGATCTGAGACAAACAGGGTACATTGAGCGAGAGGAGTTGAAGGAGATGGTGGTGGCACTTTTGCACGAATCTGATCTGGTACTCTCGGATGATGTCATTGAAATGATTGTGGATAAGACATATAGTGATGCAGATAAGAAAGGTGATGGGCGGATTGATGAAGAAGAGTGGAAGGAATTTGTGTCGAAGCATCCGAATATGATAAAGAATATGACTCTCCCGTACTTAAAAGATATAACATTAGCGTTTCCTAGCTTTGTGATGACTTCTGAAGTCGAGGATTCAGAAATGTGA

Protein Analysis

213

Amino Acids

24.39

Weight (kDa)

4.63

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 105 - 176 1.5e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 496
AcsI RAATTY 4 cut(s) 164, 269, 321, 521
AcuI CTGAAG 2 cut(s) 166, 636
AfaI GTAC 4 cut(s) 104, 365, 426, 569
AgsI TTSAA 2 cut(s) 385, 446
AjnI CCWGG 1 cut(s) 34
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 4 cut(s) 26, 109, 172, 600
AluI AGCT 4 cut(s) 26, 109, 172, 600
Alw26I GTCTC 1 cut(s) 346
ApeKI GCWGC 3 cut(s) 6, 62, 65
ApoI RAATTY 4 cut(s) 164, 269, 321, 521
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
Asp700I GAANNNNTTC 2 cut(s) 165, 273
AspLEI GCGC 2 cut(s) 178, 246
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 2 cut(s) 287, 500
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 49, 52
BccI CCATC 2 cut(s) 385, 485
BciT130I CCWGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 309
BisI GCNGC 3 cut(s) 7, 63, 66
BlsI GCNGC 3 cut(s) 8, 64, 67
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 3 cut(s) 292, 463, 544
BpiI GAAGAC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse3DI GCAATG 1 cut(s) 253
BseBI CCWGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 439, 535
BseMI GCAATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 2 cut(s) 49, 52
Bsh1236I CGCG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 346
Bsp119I TTCGAA 1 cut(s) 17
Bsp143I GATC 3 cut(s) 278, 346, 418
BspACI CCGC 1 cut(s) 496
BspCNI CTCAG 1 cut(s) 342
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 48
BspQI GCTCTTC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 253
BssMI GATC 3 cut(s) 278, 346, 418
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 82, 342
Bst6I CTCTTC 2 cut(s) 185, 504
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 439, 535
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 2 cut(s) 178, 246
BstKTI GATC 3 cut(s) 281, 349, 421
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 3 cut(s) 278, 346, 418
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 49, 52
BstV2I GAAGAC 1 cut(s) 321
BtsCI GGATG 2 cut(s) 439, 535
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 178, 246
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 4 cut(s) 103, 364, 425, 568
CviJI RGCY 7 cut(s) 6, 26, 39, 98, 109, 172, 600
CviKI_1 RGCY 7 cut(s) 6, 26, 39, 98, 109, 172, 600
CviQI GTAC 4 cut(s) 103, 364, 425, 568
DdeI CTNAG 1 cut(s) 350
DpnI GATC 3 cut(s) 280, 348, 420
DpnII GATC 3 cut(s) 278, 346, 418
Eam1104I CTCTTC 2 cut(s) 185, 504
EarI CTCTTC 2 cut(s) 185, 504
EciI GGCGGA 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 166, 636
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 34
FaiI YATR 7 cut(s) 42, 345, 466, 468, 545, 557, 581
Fnu4HI GCNGC 3 cut(s) 7, 63, 66
FokI GGATG 2 cut(s) 446, 522
Fsp4HI GCNGC 3 cut(s) 7, 63, 66
FspBI CTAG 1 cut(s) 597
GlaI GCGC 2 cut(s) 177, 245
GluI GCNGC 3 cut(s) 7, 63, 66
HhaI GCGC 2 cut(s) 178, 246
Hin6I GCGC 2 cut(s) 176, 244
HinP1I GCGC 2 cut(s) 176, 244
HinfI GANTC 5 cut(s) 55, 202, 413, 559, 626
HphI GGTGA 2 cut(s) 287, 500
Hpy188I TCNGA 6 cut(s) 351, 418, 433, 540, 616, 631
Hpy188III TCNNGA 1 cut(s) 184
HpyAV CCTTC 3 cut(s) 13, 379, 511
HpyCH4III ACNGT 2 cut(s) 82, 342
HpyCH4V TGCA 5 cut(s) 68, 212, 305, 409, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 1 cut(s) 350
HspAI GCGC 2 cut(s) 176, 244
Kzo9I GATC 3 cut(s) 278, 346, 418
LguI GCTCTTC 1 cut(s) 185
LpnPI CCDG 8 cut(s) 21, 48, 63, 169, 202, 321, 345, 407
Lsp1109I GCAGC 2 cut(s) 49, 52
LweI GCATC 3 cut(s) 292, 463, 544
MaeI CTAG 1 cut(s) 597
MalI GATC 3 cut(s) 280, 348, 420
MboI GATC 3 cut(s) 278, 346, 418
MboII GAAGA 5 cut(s) 172, 173, 326, 518, 521
MluCI AATT 6 cut(s) 119, 135, 164, 269, 321, 521
MlyI GAGTC 2 cut(s) 49, 553
MnlI CCTC 3 cut(s) 37, 370, 616
MroXI GAANNNNTTC 2 cut(s) 165, 273
MseI TTAA 2 cut(s) 114, 572
MslI CAYNNNNRTG 2 cut(s) 300, 446
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeII GATC 3 cut(s) 278, 346, 418
NlaIV GGNNCC 1 cut(s) 48
NspV TTCGAA 1 cut(s) 17
PciSI GCTCTTC 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PdmI GAANNNNTTC 2 cut(s) 165, 273
PfeI GAWTC 3 cut(s) 202, 413, 626
PkrI GCNGC 3 cut(s) 8, 64, 67
PleI GAGTC 2 cut(s) 49, 553
PpsI GAGTC 2 cut(s) 49, 553
PpuMI RGGWCCY 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 46
PspPPI RGGWCCY 1 cut(s) 46
RsaI GTAC 4 cut(s) 104, 365, 426, 569
RsaNI GTAC 4 cut(s) 103, 364, 425, 568
RseI CAYNNNNRTG 2 cut(s) 300, 446
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 114, 572
SatI GCNGC 3 cut(s) 7, 63, 66
Sau3AI GATC 3 cut(s) 278, 346, 418
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 2 cut(s) 49, 553
ScrFI CCNGG 1 cut(s) 36
SetI ASST 8 cut(s) 28, 76, 111, 174, 289, 310, 490, 602
SfaNI GCATC 3 cut(s) 292, 463, 544
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 300, 446
Sse9I AATT 6 cut(s) 119, 135, 164, 269, 321, 521
SsiI CCGC 1 cut(s) 496
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 1 cut(s) 34
TaaI ACNGT 2 cut(s) 82, 342
TaqI TCGA 5 cut(s) 17, 131, 277, 530, 621
TasI AATT 6 cut(s) 119, 135, 164, 269, 321, 521
TfiI GAWTC 3 cut(s) 202, 413, 626
Tru1I TTAA 2 cut(s) 114, 572
Tru9I TTAA 2 cut(s) 114, 572
TseI GCWGC 3 cut(s) 6, 62, 65
TspDTI ATGAA 3 cut(s) 102, 315, 519
TspGWI ACGGA 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 4 cut(s) 164, 269, 321, 521
XmnI GAANNNNTTC 2 cut(s) 165, 273
XspI CTAG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.