FvH4_7g31980

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006375
Physical Location & Seq
Reverse (-)
23068996 .. 23072637
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31980.t5

Sequence Viewer

Length: 1197 bp
ATGCCGTATTGCGAAGTCGCGTCGCCGCAGAATGCCGCCGCCGACGACGAAGCAACAAAAATCTACTACCGGACTTACGGCCGCGGCCCCATCAAGGTCCTCCTGATCATCGGATTGGCCGGAACTCACGAATCGTGGGGGCCACAGATTCAGGGGCTCACGGGGTCGGTTACGCCGAACGACGAGGATAGGCCGGCGGCGGAGTGTGGCGACAATGAGGCTGGTTTCAGCGGCGGAGGTATCGAGGTTTGCGCGTTTGATAATCGGGGCATGGGCCGGAGCTCCGTGCCGGCGAAAAAGTCCGATTACACAACAAGAACAATGGCGAAGGATGCAATCGCTTTGATGGATCATCTGGGGTGGAAGCAAGCGCATGTTTTCGGGCATTCAATGGGAGCTATGATAGCTTGTAAGTTAGCAGCGATGGTTCCTGACAGAATTTTGTCATTGGGTTTGCTCAATGTCACCGGAGGCGGTTTTGAATGTTTACCAAGGCTCGACCGCCAAACATTATCTATTGCTATTCGTTTTCTAAAGGCAAAGACTCCGGAGCAAAGAGCAGCTGTTGACTTGGACACCCACTATTCGAAGGAGTATCTTGAGGAAAATATTGGATCCAACACAAGAAGGGCAATTTTATACCAACAATATGTAAAAGGTATATCGGCTACTGGGATGCAGTCTAACCATGGTTTCGAAGGTCAACTGAATGCGTGCTGGACACATAAAATGACACCAACTGAAATTGAAATGATCCGCTCCGCTGGGTTTCTTGTTTCAGTCATTCATGGCAGGCATGATGTCATTGCTCAAATATGTTATGCAAAGAGATTGGCACAGAAGCTGCAGCCTGTTGCTAGAATGGTAGATCTTCCTGGAGGACATCTTGTGAGTCATGAGAGGACTGAAGAGGTCAATAAAGCCCTTCTTGAGTTGATCAAGGCTTCAGAAGCTAAGACAGAGCCACAGGACTGGACAAATTTGCCTGAGAAAAGTTCCGGGTGGGTGGTTACAAGGATGTCGTTTATCAAAAAAAGTAATAGCGAGGCTAACAGTGCATGTTTGGCATCCAGTATGTTGCAGAAGCTACATATGTTCCTCTTGTACTTGTTTGGTCTCTTTGCCTTGGCGTGCGAGAAAGGATTGATGACTTTCAGAAGTATAAAACCAGCTAGAGTTGCAAATTCCATTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

399

Amino Acids

43.63

Weight (kDa)

8.48

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 2 - 194 1.7e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 4 - 200 2e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 759
AccII CGCG 3 cut(s) 20, 84, 254
AccIII TCCGGA 1 cut(s) 547
AclWI GGATC 4 cut(s) 357, 609, 622, 748
AcoI YGGCCR 2 cut(s) 79, 117
AcsI RAATTY 3 cut(s) 438, 979, 1183
AcuI CTGAAG 2 cut(s) 927, 930
AfaI GTAC 1 cut(s) 1106
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 3 cut(s) 390, 482, 749
AjnI CCWGG 1 cut(s) 874
AluBI AGCT 8 cut(s) 282, 398, 407, 563, 844, 953, 1087, 1172
AluI AGCT 8 cut(s) 282, 398, 407, 563, 844, 953, 1087, 1172
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 1 cut(s) 1121
AlwI GGATC 4 cut(s) 357, 609, 622, 748
AlwNI CAGNNNCTG 1 cut(s) 844
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 6 cut(s) 79, 85, 117, 140, 191, 274
ApeKI GCWGC 4 cut(s) 419, 560, 844, 847
ApoI RAATTY 3 cut(s) 438, 979, 1183
AspLEI GCGC 2 cut(s) 254, 373
AspS9I GGNCC 4 cut(s) 86, 97, 140, 274
AsuC2I CCSGG 1 cut(s) 1000
AsuHPI GGTGA 1 cut(s) 457
AsuII TTCGAA 2 cut(s) 587, 696
AvaII GGWCC 1 cut(s) 97
BamHI GGATCC 1 cut(s) 614
BanII GRGCYC 2 cut(s) 159, 284
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 4 cut(s) 431, 572, 831, 859
BccI CCATC 3 cut(s) 98, 340, 418
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 876
BclI TGATCA 2 cut(s) 105, 936
BcnI CCSGG 1 cut(s) 1000
BcoDI GTCTC 1 cut(s) 1121
BfaI CTAG 2 cut(s) 858, 1173
BfmI CTRYAG 1 cut(s) 845
BglII AGATCT 1 cut(s) 868
Bme1390I CCNGG 2 cut(s) 876, 1000
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 86, 97, 140, 274
BmiI GGNNCC 4 cut(s) 88, 141, 429, 616
BmrFI CCNGG 2 cut(s) 876, 1000
BmrI ACTGGG 1 cut(s) 681
BmsI GCATC 3 cut(s) 322, 666, 1076
BmuI ACTGGG 1 cut(s) 681
BpmI CTGGAG 1 cut(s) 897
Bpu14I TTCGAA 2 cut(s) 587, 696
BpuEI CTTGAG 2 cut(s) 620, 950
BpuMI CCSGG 1 cut(s) 1000
BsaI GGTCTC 1 cut(s) 1121
BsaJI CCNNGG 4 cut(s) 82, 491, 688, 1125
BsaWI WCCGGW 3 cut(s) 69, 467, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse118I RCCGGY 2 cut(s) 193, 289
Bse1I ACTGG 3 cut(s) 676, 977, 1071
Bse3DI GCAATG 1 cut(s) 804
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 4 cut(s) 82, 491, 688, 1125
BseGI GGATG 4 cut(s) 337, 681, 1023, 1067
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 3 cut(s) 676, 977, 1071
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 4 cut(s) 431, 572, 831, 859
BseYI CCCAGC 1 cut(s) 764
Bsh1236I CGCG 3 cut(s) 20, 84, 254
Bsh1285I CGRYCG 2 cut(s) 82, 502
BshFI GGCC 6 cut(s) 81, 87, 119, 142, 193, 276
BsiEI CGRYCG 2 cut(s) 82, 502
BsiHKAI GWGCWC 1 cut(s) 284
BsiSI CCGG 8 cut(s) 70, 120, 194, 277, 290, 468, 548, 999
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 1121
BsmI GAATGC 3 cut(s) 37, 385, 715
BsnI GGCC 6 cut(s) 81, 87, 119, 142, 193, 276
Bso31I GGTCTC 1 cut(s) 1121
Bsp119I TTCGAA 2 cut(s) 587, 696
Bsp1286I GDGCHC 2 cut(s) 159, 284
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 6 cut(s) 105, 349, 614, 753, 868, 936
Bsp19I CCATGG 1 cut(s) 688
BspANI GGCC 6 cut(s) 81, 87, 119, 142, 193, 276
BspCNI CTCAG 1 cut(s) 979
BspEI TCCGGA 1 cut(s) 547
BspFNI CGCG 3 cut(s) 20, 84, 254
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 4 cut(s) 88, 141, 429, 616
BspMAI CTGCAG 1 cut(s) 849
BspPI GGATC 4 cut(s) 357, 609, 622, 748
BspT104I TTCGAA 2 cut(s) 587, 696
BspTNI GGTCTC 1 cut(s) 1121
BsrBI CCGCTC 1 cut(s) 759
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 2 cut(s) 193, 289
BsrI ACTGG 3 cut(s) 676, 977, 1071
BssAI RCCGGY 2 cut(s) 193, 289
BssECI CCNNGG 4 cut(s) 82, 491, 688, 1125
BssMI GATC 6 cut(s) 105, 349, 614, 753, 868, 936
BssT1I CCWWGG 3 cut(s) 491, 688, 1125
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 1 cut(s) 1055
Bst6I CTCTTC 1 cut(s) 903
BstBI TTCGAA 2 cut(s) 587, 696
BstC8I GCNNGC 6 cut(s) 195, 291, 369, 715, 794, 1132
BstDEI CTNAG 2 cut(s) 954, 987
BstDSI CCRYGG 2 cut(s) 82, 688
BstF5I GGATG 4 cut(s) 337, 681, 1023, 1067
BstFNI CGCG 3 cut(s) 20, 84, 254
BstHHI GCGC 2 cut(s) 254, 373
BstKTI GATC 6 cut(s) 108, 352, 617, 756, 871, 939
BstMAI GTCTC 1 cut(s) 1121
BstMBI GATC 6 cut(s) 105, 349, 614, 753, 868, 936
BstMCI CGRYCG 2 cut(s) 82, 502
BstMWI GCNNNNNNNGC 6 cut(s) 332, 404, 950, 1055, 1064, 1178
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 2 cut(s) 377, 1062
BstSCI CCNGG 2 cut(s) 874, 998
BstSFI CTRYAG 1 cut(s) 845
BstUI CGCG 3 cut(s) 20, 84, 254
BstV1I GCAGC 4 cut(s) 431, 572, 831, 859
BstX2I RGATCY 2 cut(s) 614, 868
BstXI CCANNNNNNTGG 1 cut(s) 972
BstYI RGATCY 2 cut(s) 614, 868
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 6 cut(s) 81, 87, 119, 142, 193, 276
BtgI CCRYGG 2 cut(s) 82, 688
BtgZI GCGATG 1 cut(s) 437
BtsCI GGATG 4 cut(s) 337, 681, 1023, 1067
BtsIMutI CAGTG 1 cut(s) 1060
Cac8I GCNNGC 6 cut(s) 195, 291, 369, 715, 794, 1132
CaiI CAGNNNCTG 1 cut(s) 844
CciI TCATGA 1 cut(s) 895
CfoI GCGC 2 cut(s) 254, 373
Cfr10I RCCGGY 2 cut(s) 193, 289
Cfr13I GGNCC 4 cut(s) 86, 97, 140, 274
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 1 cut(s) 9
Csp6I GTAC 1 cut(s) 1105
CviAII CATG 7 cut(s) 271, 374, 689, 788, 797, 896, 1059
CviQI GTAC 1 cut(s) 1105
DdeI CTNAG 2 cut(s) 954, 987
DpnI GATC 6 cut(s) 107, 351, 616, 755, 870, 938
DpnII GATC 6 cut(s) 105, 349, 614, 753, 868, 936
EaeI YGGCCR 2 cut(s) 79, 117
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
EciI GGCGGA 2 cut(s) 215, 249
Ecl136II GAGCTC 1 cut(s) 282
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 3 cut(s) 491, 688, 1125
Eco24I GRGCYC 2 cut(s) 159, 284
Eco31I GGTCTC 1 cut(s) 1121
Eco47I GGWCC 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 2 cut(s) 927, 930
EcoICRI GAGCTC 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 874
EcoT14I CCWWGG 3 cut(s) 491, 688, 1125
EcoT38I GRGCYC 2 cut(s) 159, 284
ErhI CCWWGG 3 cut(s) 491, 688, 1125
FaeI CATG 7 cut(s) 274, 377, 692, 791, 800, 899, 1062
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FatI CATG 7 cut(s) 270, 373, 688, 787, 796, 895, 1058
FauNDI CATATG 1 cut(s) 1092
FbaI TGATCA 2 cut(s) 105, 936
FokI GGATG 4 cut(s) 344, 688, 1030, 1054
FriOI GRGCYC 2 cut(s) 159, 284
FspBI CTAG 2 cut(s) 858, 1173
GlaI GCGC 2 cut(s) 253, 372
GsaI CCCAGC 1 cut(s) 768
GsuI CTGGAG 1 cut(s) 897
HaeIII GGCC 6 cut(s) 81, 87, 119, 142, 193, 276
HapII CCGG 8 cut(s) 70, 120, 194, 277, 290, 468, 548, 999
HgaI GACGC 1 cut(s) 9
HhaI GCGC 2 cut(s) 254, 373
Hin1II CATG 7 cut(s) 274, 377, 692, 791, 800, 899, 1062
Hin6I GCGC 2 cut(s) 252, 371
HinP1I GCGC 2 cut(s) 252, 371
HincII GTYRAC 2 cut(s) 568, 704
HindII GTYRAC 2 cut(s) 568, 704
HinfI GANTC 4 cut(s) 131, 148, 544, 892
HpaII CCGG 8 cut(s) 70, 120, 194, 277, 290, 468, 548, 999
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 3 cut(s) 488, 568, 704
Hpy188I TCNGA 4 cut(s) 113, 304, 949, 1157
Hpy188III TCNNGA 7 cut(s) 103, 128, 431, 548, 599, 896, 929
Hpy8I GTNNAC 3 cut(s) 488, 568, 704
Hpy99I CGWCG 4 cut(s) 25, 47, 50, 185
HpyAV CCTTC 5 cut(s) 322, 583, 621, 692, 935
HpyCH4III ACNGT 1 cut(s) 1055
HpyCH4V TGCA 7 cut(s) 335, 679, 824, 847, 1058, 1081, 1181
HpyF10VI GCNNNNNNNGC 6 cut(s) 332, 404, 950, 1055, 1064, 1178
HpyF3I CTNAG 2 cut(s) 954, 987
Hsp92II CATG 7 cut(s) 274, 377, 692, 791, 800, 899, 1062
HspAI GCGC 2 cut(s) 252, 371
Kpn2I TCCGGA 1 cut(s) 547
KroI GCCGGC 2 cut(s) 193, 289
KroNI GCCGGC 2 cut(s) 195, 291
Ksp22I TGATCA 2 cut(s) 105, 936
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 6 cut(s) 105, 349, 614, 753, 868, 936
LmnI GCTCC 5 cut(s) 279, 287, 395, 550, 764
Lsp1109I GCAGC 4 cut(s) 431, 572, 831, 859
LweI GCATC 3 cut(s) 322, 666, 1076
MaeI CTAG 2 cut(s) 858, 1173
MaeIII GTNAC 3 cut(s) 169, 463, 1009
MalI GATC 6 cut(s) 107, 351, 616, 755, 870, 938
MbiI CCGCTC 1 cut(s) 759
MboI GATC 6 cut(s) 105, 349, 614, 753, 868, 936
MboII GAAGA 2 cut(s) 863, 920
MflI RGATCY 2 cut(s) 614, 868
MhlI GDGCHC 2 cut(s) 159, 284
MluCI AATT 5 cut(s) 438, 633, 744, 979, 1183
MlyI GAGTC 2 cut(s) 538, 901
MmeI TCCRAC 1 cut(s) 642
MroI TCCGGA 1 cut(s) 547
MroNI GCCGGC 2 cut(s) 193, 289
MspA1I CMGCKG 4 cut(s) 84, 231, 563, 764
MspI CCGG 8 cut(s) 70, 120, 194, 277, 290, 468, 548, 999
MspR9I CCNGG 2 cut(s) 876, 1000
Mva1269I GAATGC 3 cut(s) 37, 385, 715
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 3 cut(s) 20, 84, 254
MwoI GCNNNNNNNGC 6 cut(s) 332, 404, 950, 1055, 1064, 1178
NaeI GCCGGC 2 cut(s) 195, 291
NciI CCSGG 1 cut(s) 1000
NcoI CCATGG 1 cut(s) 688
NdeI CATATG 1 cut(s) 1092
NdeII GATC 6 cut(s) 105, 349, 614, 753, 868, 936
NgoMIV GCCGGC 2 cut(s) 193, 289
NlaIII CATG 7 cut(s) 274, 377, 692, 791, 800, 899, 1062
NlaIV GGNNCC 4 cut(s) 88, 141, 429, 616
NmuCI GTSAC 1 cut(s) 463
NspI RCATGY 2 cut(s) 377, 1062
NspV TTCGAA 2 cut(s) 587, 696
PagI TCATGA 1 cut(s) 895
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PctI GAATGC 3 cut(s) 37, 385, 715
PdiI GCCGGC 2 cut(s) 195, 291
PfeI GAWTC 2 cut(s) 131, 148
PfoI TCCNGGA 1 cut(s) 874
PleI GAGTC 2 cut(s) 538, 900
PpsI GAGTC 2 cut(s) 538, 900
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 284
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 4 cut(s) 88, 141, 429, 616
PspPI GGNCC 4 cut(s) 86, 97, 140, 274
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 849
PstNI CAGNNNCTG 1 cut(s) 844
PsuI RGATCY 2 cut(s) 614, 868
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 1106
RsaNI GTAC 1 cut(s) 1105
SacI GAGCTC 1 cut(s) 284
SacII CCGCGG 1 cut(s) 85
Sau3AI GATC 6 cut(s) 105, 349, 614, 753, 868, 936
Sau96I GGNCC 4 cut(s) 86, 97, 140, 274
SchI GAGTC 2 cut(s) 538, 901
ScrFI CCNGG 2 cut(s) 876, 1000
SduI GDGCHC 2 cut(s) 159, 284
SfaNI GCATC 3 cut(s) 322, 666, 1076
SfcI CTRYAG 1 cut(s) 845
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 587, 696
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 97
SmlI CTYRAG 2 cut(s) 599, 929
SmoI CTYRAG 2 cut(s) 599, 929
Sse9I AATT 5 cut(s) 438, 633, 744, 979, 1183
SspI AATATT 1 cut(s) 610
SspMI CTAG 2 cut(s) 858, 1173
SstI GAGCTC 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 874, 998
StyI CCWWGG 3 cut(s) 491, 688, 1125
TaaI ACNGT 1 cut(s) 1055
TaqI TCGA 4 cut(s) 243, 498, 587, 696
TasI AATT 5 cut(s) 438, 633, 744, 979, 1183
TatI WGTACW 1 cut(s) 1104
TauI GCSGC 7 cut(s) 28, 38, 41, 84, 87, 200, 234
TfiI GAWTC 2 cut(s) 131, 148
TscAI CASTG 1 cut(s) 1060
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 4 cut(s) 419, 560, 844, 847
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 1 cut(s) 776
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 1 cut(s) 1060
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 3 cut(s) 438, 979, 1183
XceI RCATGY 2 cut(s) 377, 1062
XspI CTAG 2 cut(s) 858, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.