Rh1AG452100

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006375
Physical Location & Seq
Reverse (-)
67216425 .. 67219817
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG452100.1

Sequence Viewer

Length: 1215 bp
ATGCCGTTTTGCGAAGTCGCGTCGCCGCAGAATAATGCCGCCGCAGTCGACGCCAAGCTCAACGACGCCGGAACCAAAATTTACTACCGGACTTACGGCCGCGGGCCCACCAAGGTCCTCCTGATCATCGGATTGGCGGGAACTCACGATTCGTGGGGGCCACAGATACAGGGGCTGACGGGGACGGTTACGGCGAACGACGATGATAGGGCGGCGGAGGAGTGTGGCGATAATGAGGCTGGTTTTAGCGGCGGAGGGATCGAGGTGTGCGCGTTTGATAATCGGGGGATGGGTCGGAGCTCCGTGCCGGCGAAAAAGTCAGAATATACAACAAGAACAATGGCGAAAGATGCAATCGCTTTGATGGATCATTTGGGGTGGAAACAAGCGCATGTTTTCGGGCATTCAATGGGAGCTATGATAGCTTGCAAGTTAGCAGCGATGGTGTCCGACAGAGTTTTGTCATTGGCTCTGCTCAATGTGACCGGAGGAGGTTTCGAGTGTTTACCAAGGCTCGACCGCCAAACATTATCTATTGCCATCCGTTTTTTGAAGGCAAAGACGCCTGAGCAAAGAGCAGCTGTTGACTTGGACACGCACTATTCAAAGGGGTATCTTGAGGAATACGTTGGATCCAACACAAGAAGGGCAATTTTATACCAAGAATATGTAAAAGGTATATCAGCAACTGGGATGCAGTCTAACCATGGTTTTGAAGGTCAACTGAATGCGTGCTGGACACATAAAATGACACCAACTGAAATTGAAAAGATCCGCTCTGCTGGATTTCTTGTTTCAGTCATTCATGGCAGGCATGATGTCATCGCTCAAATATATTATGCAAAGAGACTGGCGCAGAAGCTGCAGCCTGTTGCTAGAATGGTTGATCTTCCTGGAGGGCATCTCGTGAGTCATGAGAGGACTGAAGAGGTCAATAATGCTCTTAATGAGTTGATCAAAGCTTCAGAAGCTAAGACAGAGCCACATGATTGGACAAATTTGCCTGAGAAAAGTTCCGGGTGGGTGGTTACAAGGATGTTGTTTGTCAAAAAAGGTAATGGTGAGGTTAATAGTGCATCTTTGGCATCCAGTATGTTACAAAAGCTACATATTTTCCTCTTGTACTTCTTTGGTCTCTTTGCCTTGGCATTCGAGAAGGGGATGATGACTTTCAGAAGATTAAAACCAGCTAGAGTTGCAAACTCCATCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

404

Amino Acids

44.29

Weight (kDa)

8.87

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 39 - 306 1.8e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 83 - 313 2.8e-12 Alpha/beta hydrolase family
Hydrolase_4 PF12146 83 - 159 1.1e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 777
AccI GTMKAC 1 cut(s) 48
AccII CGCG 3 cut(s) 20, 102, 272
AclWI GGATC 5 cut(s) 266, 375, 627, 640, 766
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 78, 997
AcuI CTGAAG 2 cut(s) 945, 948
AcyI GRCGYC 3 cut(s) 51, 66, 563
AfaI GTAC 1 cut(s) 1124
AgsI TTSAA 5 cut(s) 408, 553, 606, 716, 767
AjnI CCWGG 1 cut(s) 892
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 2 cut(s) 841, 1139
AlwI GGATC 5 cut(s) 266, 375, 627, 640, 766
AlwNI CAGNNNCTG 3 cut(s) 175, 689, 862
AoxI GGCC 3 cut(s) 97, 104, 158
ApaI GGGCCC 1 cut(s) 108
ApeKI GCWGC 4 cut(s) 437, 578, 862, 865
ApoI RAATTY 2 cut(s) 78, 997
AspLEI GCGC 3 cut(s) 272, 391, 856
AspS9I GGNCC 4 cut(s) 104, 105, 115, 158
AsuC2I CCSGG 1 cut(s) 1018
AsuHPI GGTGA 1 cut(s) 1073
AvaII GGWCC 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 108
BamHI GGATCC 1 cut(s) 632
BanII GRGCYC 2 cut(s) 108, 302
BauI CACGAG 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 302
BbvI GCAGC 4 cut(s) 449, 590, 849, 877
BccI CCATC 5 cut(s) 283, 358, 436, 548, 1214
BceAI ACGGC 2 cut(s) 112, 207
BciT130I CCWGG 1 cut(s) 894
BclI TGATCA 2 cut(s) 123, 954
BcnI CCSGG 1 cut(s) 1018
BcoDI GTCTC 2 cut(s) 841, 1139
BfaI CTAG 2 cut(s) 876, 1191
BfmI CTRYAG 1 cut(s) 863
Bme1390I CCNGG 2 cut(s) 894, 1018
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 104, 105, 115, 158
BmiI GGNNCC 4 cut(s) 73, 106, 159, 634
BmrFI CCNGG 2 cut(s) 894, 1018
BmrI ACTGGG 1 cut(s) 699
BmsI GCATC 5 cut(s) 340, 684, 910, 1085, 1094
BmuI ACTGGG 1 cut(s) 699
BplI GAGNNNNNCTC 2 cut(s) 888, 920
BpmI CTGGAG 1 cut(s) 915
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 638
BpuMI CCSGG 1 cut(s) 1018
BsaHI GRCGYC 3 cut(s) 51, 66, 563
BsaI GGTCTC 1 cut(s) 1139
BsaJI CCNNGG 5 cut(s) 100, 111, 509, 706, 1143
BsaWI WCCGGW 2 cut(s) 87, 485
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 3 cut(s) 694, 855, 1089
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 5 cut(s) 100, 111, 509, 706, 1143
BseGI GGATG 6 cut(s) 294, 540, 699, 1041, 1085, 1167
BseMII CTCAG 2 cut(s) 558, 996
BseNI ACTGG 3 cut(s) 694, 855, 1089
BseRI GAGGAG 2 cut(s) 233, 504
BseSI GKGCMC 1 cut(s) 108
BseX3I CGGCCG 1 cut(s) 97
BseXI GCAGC 4 cut(s) 449, 590, 849, 877
Bsh1236I CGCG 3 cut(s) 20, 102, 272
Bsh1285I CGRYCG 2 cut(s) 100, 520
BshFI GGCC 3 cut(s) 99, 106, 160
BsiEI CGRYCG 2 cut(s) 100, 520
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 5 cut(s) 69, 88, 308, 486, 1017
BslFI GGGAC 1 cut(s) 196
BsmAI GTCTC 2 cut(s) 841, 1139
BsmFI GGGAC 1 cut(s) 196
BsmI GAATGC 3 cut(s) 403, 733, 1148
BsnI GGCC 3 cut(s) 99, 106, 160
Bso31I GGTCTC 1 cut(s) 1139
Bsp120I GGGCCC 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 108, 302
Bsp143I GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
Bsp19I CCATGG 1 cut(s) 706
BspANI GGCC 3 cut(s) 99, 106, 160
BspCNI CTCAG 2 cut(s) 559, 997
BspFNI CGCG 3 cut(s) 20, 102, 272
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 4 cut(s) 73, 106, 159, 634
BspMAI CTGCAG 1 cut(s) 867
BspPI GGATC 5 cut(s) 266, 375, 627, 640, 766
BspTNI GGTCTC 1 cut(s) 1139
BsrBI CCGCTC 1 cut(s) 777
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 3 cut(s) 694, 855, 1089
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 5 cut(s) 100, 111, 509, 706, 1143
BssMI GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
BssNI GRCGYC 3 cut(s) 51, 66, 563
BssSI CACGAG 1 cut(s) 905
BssT1I CCWWGG 4 cut(s) 111, 509, 706, 1143
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 921
BstACI GRCGYC 3 cut(s) 51, 66, 563
BstAPI GCANNNNNTGC 1 cut(s) 862
BstC8I GCNNGC 5 cut(s) 104, 309, 427, 733, 812
BstDEI CTNAG 3 cut(s) 567, 972, 1005
BstDSI CCRYGG 2 cut(s) 100, 706
BstF5I GGATG 6 cut(s) 294, 540, 699, 1041, 1085, 1167
BstFNI CGCG 3 cut(s) 20, 102, 272
BstHHI GCGC 3 cut(s) 272, 391, 856
BstKTI GATC 7 cut(s) 126, 261, 370, 635, 774, 889, 957
BstMAI GTCTC 2 cut(s) 841, 1139
BstMBI GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
BstMCI CGRYCG 2 cut(s) 100, 520
BstMWI GCNNNNNNNGC 7 cut(s) 50, 350, 422, 862, 968, 1082, 1196
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 1 cut(s) 395
BstSCI CCNGG 2 cut(s) 892, 1016
BstSFI CTRYAG 1 cut(s) 863
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 3 cut(s) 20, 102, 272
BstV1I GCAGC 4 cut(s) 449, 590, 849, 877
BstX2I RGATCY 2 cut(s) 632, 771
BstXI CCANNNNNNTGG 1 cut(s) 990
BstYI RGATCY 2 cut(s) 632, 771
BstZI CGGCCG 1 cut(s) 97
BsuRI GGCC 3 cut(s) 99, 106, 160
BtgI CCRYGG 2 cut(s) 100, 706
BtgZI GCGATG 2 cut(s) 455, 808
BtsCI GGATG 6 cut(s) 294, 540, 699, 1041, 1085, 1167
Cac8I GCNNGC 5 cut(s) 104, 309, 427, 733, 812
CaiI CAGNNNCTG 3 cut(s) 175, 689, 862
CciI TCATGA 1 cut(s) 913
CfoI GCGC 3 cut(s) 272, 391, 856
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 4 cut(s) 104, 105, 115, 158
Cfr42I CCGCGG 1 cut(s) 103
CseI GACGC 4 cut(s) 9, 59, 74, 571
Csp6I GTAC 1 cut(s) 1123
CviAII CATG 6 cut(s) 392, 707, 806, 815, 914, 986
CviQI GTAC 1 cut(s) 1123
DdeI CTNAG 3 cut(s) 567, 972, 1005
DpnI GATC 7 cut(s) 125, 260, 369, 634, 773, 888, 956
DpnII GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
EaeI YGGCCR 1 cut(s) 97
EagI CGGCCG 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
EciI GGCGGA 2 cut(s) 230, 267
Ecl136II GAGCTC 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 97
Eco130I CCWWGG 4 cut(s) 111, 509, 706, 1143
Eco24I GRGCYC 2 cut(s) 108, 302
Eco31I GGTCTC 1 cut(s) 1139
Eco47I GGWCC 1 cut(s) 115
Eco52I CGGCCG 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 300
Eco57I CTGAAG 2 cut(s) 945, 948
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 892
EcoT14I CCWWGG 4 cut(s) 111, 509, 706, 1143
EcoT38I GRGCYC 2 cut(s) 108, 302
ErhI CCWWGG 4 cut(s) 111, 509, 706, 1143
FaeI CATG 6 cut(s) 395, 710, 809, 818, 917, 989
FaqI GGGAC 1 cut(s) 196
FatI CATG 6 cut(s) 391, 706, 805, 814, 913, 985
FauI CCCGC 2 cut(s) 95, 130
FbaI TGATCA 2 cut(s) 123, 954
FblI GTMKAC 1 cut(s) 48
FokI GGATG 6 cut(s) 301, 527, 706, 1048, 1072, 1174
FriOI GRGCYC 2 cut(s) 108, 302
FspBI CTAG 2 cut(s) 876, 1191
GlaI GCGC 3 cut(s) 271, 390, 855
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 3 cut(s) 99, 106, 160
HapII CCGG 5 cut(s) 69, 88, 308, 486, 1017
HgaI GACGC 4 cut(s) 9, 59, 74, 571
HhaI GCGC 3 cut(s) 272, 391, 856
Hin1I GRCGYC 3 cut(s) 51, 66, 563
Hin1II CATG 6 cut(s) 395, 710, 809, 818, 917, 989
Hin6I GCGC 3 cut(s) 270, 389, 854
HinP1I GCGC 3 cut(s) 270, 389, 854
HincII GTYRAC 3 cut(s) 49, 586, 722
HindII GTYRAC 3 cut(s) 49, 586, 722
HindIII AAGCTT 1 cut(s) 960
HinfI GANTC 2 cut(s) 149, 910
HpaII CCGG 5 cut(s) 69, 88, 308, 486, 1017
HphI GGTGA 1 cut(s) 1073
Hpy166II GTNNAC 4 cut(s) 49, 506, 586, 722
Hpy188I TCNGA 6 cut(s) 131, 297, 322, 451, 967, 1175
Hpy188III TCNNGA 6 cut(s) 121, 146, 617, 907, 914, 1153
Hpy8I GTNNAC 4 cut(s) 49, 506, 586, 722
Hpy99I CGWCG 4 cut(s) 25, 53, 68, 203
HpyAV CCTTC 4 cut(s) 547, 639, 710, 1150
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 7 cut(s) 353, 429, 697, 842, 865, 1076, 1199
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 350, 422, 862, 968, 1082, 1196
HpyF3I CTNAG 3 cut(s) 567, 972, 1005
HpySE526I ACGT 1 cut(s) 627
Hsp92I GRCGYC 3 cut(s) 51, 66, 563
Hsp92II CATG 6 cut(s) 395, 710, 809, 818, 917, 989
HspAI GCGC 3 cut(s) 270, 389, 854
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Ksp22I TGATCA 2 cut(s) 123, 954
KspI CCGCGG 1 cut(s) 103
Kzo9I GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
LmnI GCTCC 3 cut(s) 297, 305, 413
Lsp1109I GCAGC 4 cut(s) 449, 590, 849, 877
LweI GCATC 5 cut(s) 340, 684, 910, 1085, 1094
MaeI CTAG 2 cut(s) 876, 1191
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 4 cut(s) 187, 481, 1027, 1095
MalI GATC 7 cut(s) 125, 260, 369, 634, 773, 888, 956
MbiI CCGCTC 1 cut(s) 777
MboI GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
MboII GAAGA 3 cut(s) 881, 938, 1188
MflI RGATCY 2 cut(s) 632, 771
MhlI GDGCHC 2 cut(s) 108, 302
MluCI AATT 4 cut(s) 78, 651, 762, 997
MlyI GAGTC 1 cut(s) 919
MmeI TCCRAC 4 cut(s) 275, 474, 610, 660
MroNI GCCGGC 1 cut(s) 307
MseI TTAA 3 cut(s) 945, 1068, 1181
MspA1I CMGCKG 2 cut(s) 102, 581
MspI CCGG 5 cut(s) 69, 88, 308, 486, 1017
MspR9I CCNGG 2 cut(s) 894, 1018
Mva1269I GAATGC 3 cut(s) 403, 733, 1148
MvaI CCWGG 1 cut(s) 894
MvnI CGCG 3 cut(s) 20, 102, 272
MwoI GCNNNNNNNGC 7 cut(s) 50, 350, 422, 862, 968, 1082, 1196
NaeI GCCGGC 1 cut(s) 309
NciI CCSGG 1 cut(s) 1018
NcoI CCATGG 1 cut(s) 706
NdeII GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 6 cut(s) 395, 710, 809, 818, 917, 989
NlaIV GGNNCC 4 cut(s) 73, 106, 159, 634
NmuCI GTSAC 1 cut(s) 481
NspI RCATGY 1 cut(s) 395
PagI TCATGA 1 cut(s) 913
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PctI GAATGC 3 cut(s) 403, 733, 1148
PdiI GCCGGC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 149
PfoI TCCNGGA 1 cut(s) 892
PleI GAGTC 1 cut(s) 918
PpsI GAGTC 1 cut(s) 918
PpuMI RGGWCCY 1 cut(s) 115
Psp124BI GAGCTC 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 892
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 4 cut(s) 73, 106, 159, 634
PspOMI GGGCCC 1 cut(s) 104
PspPI GGNCC 4 cut(s) 104, 105, 115, 158
PspPPI RGGWCCY 1 cut(s) 115
PstI CTGCAG 1 cut(s) 867
PstNI CAGNNNCTG 3 cut(s) 175, 689, 862
PsuI RGATCY 2 cut(s) 632, 771
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 1124
RsaNI GTAC 1 cut(s) 1123
SacI GAGCTC 1 cut(s) 302
SacII CCGCGG 1 cut(s) 103
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 945, 1068, 1181
Sau3AI GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
Sau96I GGNCC 4 cut(s) 104, 105, 115, 158
SchI GAGTC 1 cut(s) 919
ScrFI CCNGG 2 cut(s) 894, 1018
SduI GDGCHC 2 cut(s) 108, 302
SfaNI GCATC 5 cut(s) 340, 684, 910, 1085, 1094
SfcI CTRYAG 1 cut(s) 863
Sfr303I CCGCGG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 103
SinI GGWCC 1 cut(s) 115
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 4 cut(s) 78, 651, 762, 997
SspMI CTAG 2 cut(s) 876, 1191
SstI GAGCTC 1 cut(s) 302
StyD4I CCNGG 2 cut(s) 892, 1016
StyI CCWWGG 4 cut(s) 111, 509, 706, 1143
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 1 cut(s) 630
TaqI TCGA 5 cut(s) 48, 261, 498, 516, 1152
TasI AATT 4 cut(s) 78, 651, 762, 997
TatI WGTACW 1 cut(s) 1122
TauI GCSGC 6 cut(s) 28, 41, 44, 102, 215, 252
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 3 cut(s) 945, 1068, 1181
Tru9I TTAA 3 cut(s) 945, 1068, 1181
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 4 cut(s) 437, 578, 862, 865
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 1 cut(s) 794
TspGWI ACGGA 2 cut(s) 292, 533
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 2 cut(s) 78, 997
XceI RCATGY 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 876, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.