Rh1DG440600

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006375
Physical Location & Seq
Reverse (-)
65099738 .. 65103712
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG440600.1

Sequence Viewer

Length: 1212 bp
ATGCCGTTTTGCGAAGTCGCGTCGCCGCGGAATGCCGCCGCAGTCGACGCCAAGCTCAACGACGCCGGAACCAAAATTTACTACCGGACTTACGGCCGCGGGCCCACCAAGGTCCTCCTGATCATCGGATTGGCGGGAACTCACGATTCGTGGGGGCCACAGATACAGGGGCTGACGGGGACGGTGACGGCGAACGACGATGATAGGGCGGCGGAGGAGTGTGGCGACAATGAGGCTGGTTTTAGCGGCGGAGGGATCGAGGTGTGTGCGTTTGATAATCGGGGCATGGGTCGGAGCTCCGTGCCGGTGAAAAAGTCAGAATATACAACAAGAACAATGGCGAAGGATGCAATTGCTTTGATGGATCATTTGGGGTGGAAACAAGCGCATGTTTTCGGGCATTCAATGGGAGCTATGATAGCTTGCAAGTTAGTAGCGATGGTGCCCGACAGAATTTTGTCATTGGCTCTGCTCAATGTGACCGGAGGAGGTTTCGAGTGTTTACCAAGGCTCGACCGCCAAACGTTATCTATTGCCATCCGTTTTTTGAAGGCAAAGACGCCTGAGCAAAGAGCAGCTGTTGACTTGGACACGCACTATTCAAAGGGGTATCTTGAGGAATATGTTGGATCCAACACAAGAAGGGCAATTTTATACCAAGAATATGTAAAAGGTATATCAGCAACTGGGATGCAGTCTAACCATGGTTTTGAAGGTCAACTGAATGCGTGCTGGACACATAAAATGACACCAACTGAAATTGAAAAGATCCGCTCTGCTGGATTTCTTGTTTCAGTCATTCATGGCAGGCATGATGTCATCGCTCAAATATATTATGCAAAGAGACTGGCGCAGAAGCTGCAGCCTGTTGCTAGAATGGTTGATCTTCCTGGAGGGCATCTCGTGAGTCATGAGAGGACTGAAGAGGTCAATAATGCTCTTAATGAGTTGATCAAAGCTTCAGAAGCTAAGACAGAGCCACATGATTGGACAAATTTGCCTGAGAAAAGTTCCGGGTGGGTGGTTACAAGGACGTTGTTTGTCAAAAAAAGTAATGGTGAGGTTAATAGTGCATCTTTGGCATCCAGTATGTTACAAAAGCTACATATTTTCCTCTTGTACTTCTTTGGTCTCTTTGCCATGGCATTCGAGAAGGGGATGATGACTTTCAGAAGATTAAAACCAGCTAGAGTTGCAAACTCCATCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

403

Amino Acids

44.3

Weight (kDa)

8.97

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 38 - 305 2.4e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 82 - 312 1.8e-11 Alpha/beta hydrolase family
Hydrolase_4 PF12146 82 - 159 5.9e-10 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AccBSI CCGCTC 1 cut(s) 774
AccI GTMKAC 1 cut(s) 45
AccII CGCG 3 cut(s) 20, 28, 99
AclI AACGTT 1 cut(s) 524
AclWI GGATC 5 cut(s) 263, 372, 624, 637, 763
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 75, 453, 994
AcuI CTGAAG 2 cut(s) 942, 945
AcyI GRCGYC 3 cut(s) 48, 63, 560
AfaI GTAC 1 cut(s) 1121
AgsI TTSAA 5 cut(s) 405, 550, 603, 713, 764
AjnI CCWGG 1 cut(s) 889
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 2 cut(s) 838, 1136
AlwI GGATC 5 cut(s) 263, 372, 624, 637, 763
AlwNI CAGNNNCTG 3 cut(s) 172, 686, 859
AoxI GGCC 3 cut(s) 94, 101, 155
ApaI GGGCCC 1 cut(s) 105
ApeKI GCWGC 3 cut(s) 575, 859, 862
ApoI RAATTY 3 cut(s) 75, 453, 994
AspLEI GCGC 2 cut(s) 388, 853
AspS9I GGNCC 4 cut(s) 101, 102, 112, 155
AsuC2I CCSGG 1 cut(s) 1015
AsuHPI GGTGA 3 cut(s) 196, 319, 1070
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 2 cut(s) 105, 447
BamHI GGATCC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 442
BanII GRGCYC 2 cut(s) 105, 299
BauI CACGAG 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 3 cut(s) 587, 846, 874
BccI CCATC 4 cut(s) 355, 433, 545, 1211
BceAI ACGGC 2 cut(s) 109, 204
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 891
BclI TGATCA 2 cut(s) 120, 951
BcnI CCSGG 1 cut(s) 1015
BcoDI GTCTC 2 cut(s) 838, 1136
BfaI CTAG 2 cut(s) 873, 1188
BfmI CTRYAG 1 cut(s) 860
BisI GCNGC 9 cut(s) 26, 36, 39, 97, 210, 247, 576, 860, 863
BlsI GCNGC 9 cut(s) 27, 37, 40, 98, 211, 248, 577, 861, 864
Bme1390I CCNGG 2 cut(s) 891, 1015
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 101, 102, 112, 155
BmiI GGNNCC 5 cut(s) 70, 103, 156, 444, 631
BmrFI CCNGG 2 cut(s) 891, 1015
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 5 cut(s) 337, 681, 907, 1082, 1091
BmuI ACTGGG 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 885, 917
BpmI CTGGAG 1 cut(s) 912
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 1015
BsaHI GRCGYC 3 cut(s) 48, 63, 560
BsaI GGTCTC 1 cut(s) 1136
BsaJI CCNNGG 6 cut(s) 26, 97, 108, 506, 703, 1140
BsaWI WCCGGW 2 cut(s) 84, 482
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 3 cut(s) 691, 852, 1086
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 6 cut(s) 26, 97, 108, 506, 703, 1140
BseGI GGATG 5 cut(s) 352, 537, 696, 1082, 1164
BseMII CTCAG 2 cut(s) 555, 993
BseNI ACTGG 3 cut(s) 691, 852, 1086
BseRI GAGGAG 2 cut(s) 230, 501
BseSI GKGCMC 2 cut(s) 105, 447
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 3 cut(s) 587, 846, 874
Bsh1236I CGCG 3 cut(s) 20, 28, 99
Bsh1285I CGRYCG 2 cut(s) 97, 517
BshFI GGCC 3 cut(s) 96, 103, 157
BshNI GGYRCC 1 cut(s) 442
BsiEI CGRYCG 2 cut(s) 97, 517
BsiHKAI GWGCWC 1 cut(s) 299
BsiSI CCGG 5 cut(s) 66, 85, 305, 483, 1014
BslFI GGGAC 1 cut(s) 193
BsmAI GTCTC 2 cut(s) 838, 1136
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 4 cut(s) 37, 400, 730, 1145
BsnI GGCC 3 cut(s) 96, 103, 157
Bso31I GGTCTC 1 cut(s) 1136
Bsp120I GGGCCC 1 cut(s) 101
Bsp1286I GDGCHC 3 cut(s) 105, 299, 447
Bsp143I GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
Bsp19I CCATGG 2 cut(s) 703, 1140
BspANI GGCC 3 cut(s) 96, 103, 157
BspCNI CTCAG 2 cut(s) 556, 994
BspFNI CGCG 3 cut(s) 20, 28, 99
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 5 cut(s) 70, 103, 156, 444, 631
BspMAI CTGCAG 1 cut(s) 864
BspPI GGATC 5 cut(s) 263, 372, 624, 637, 763
BspT107I GGYRCC 1 cut(s) 442
BspTNI GGTCTC 1 cut(s) 1136
BsrBI CCGCTC 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 3 cut(s) 691, 852, 1086
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 6 cut(s) 26, 97, 108, 506, 703, 1140
BssMI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
BssNI GRCGYC 3 cut(s) 48, 63, 560
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 4 cut(s) 108, 506, 703, 1140
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 918
BstACI GRCGYC 3 cut(s) 48, 63, 560
BstAPI GCANNNNNTGC 1 cut(s) 859
BstC8I GCNNGC 4 cut(s) 101, 424, 730, 809
BstDEI CTNAG 3 cut(s) 564, 969, 1002
BstDSI CCRYGG 4 cut(s) 26, 97, 703, 1140
BstF5I GGATG 5 cut(s) 352, 537, 696, 1082, 1164
BstFNI CGCG 3 cut(s) 20, 28, 99
BstHHI GCGC 2 cut(s) 388, 853
BstKTI GATC 7 cut(s) 123, 258, 367, 632, 771, 886, 954
BstMAI GTCTC 2 cut(s) 838, 1136
BstMBI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
BstMCI CGRYCG 2 cut(s) 97, 517
BstMWI GCNNNNNNNGC 7 cut(s) 47, 347, 419, 859, 965, 1079, 1193
BstNI CCWGG 1 cut(s) 891
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 889, 1013
BstSFI CTRYAG 1 cut(s) 860
BstSLI GKGCMC 2 cut(s) 105, 447
BstUI CGCG 3 cut(s) 20, 28, 99
BstV1I GCAGC 3 cut(s) 587, 846, 874
BstX2I RGATCY 2 cut(s) 629, 768
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 2 cut(s) 629, 768
BstZI CGGCCG 1 cut(s) 94
BsuRI GGCC 3 cut(s) 96, 103, 157
BtgI CCRYGG 4 cut(s) 26, 97, 703, 1140
BtgZI GCGATG 2 cut(s) 452, 805
BtsCI GGATG 5 cut(s) 352, 537, 696, 1082, 1164
Cac8I GCNNGC 4 cut(s) 101, 424, 730, 809
CaiI CAGNNNCTG 3 cut(s) 172, 686, 859
CciI TCATGA 1 cut(s) 910
CfoI GCGC 2 cut(s) 388, 853
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 4 cut(s) 101, 102, 112, 155
Cfr42I CCGCGG 2 cut(s) 29, 100
CseI GACGC 4 cut(s) 9, 56, 71, 568
Csp6I GTAC 1 cut(s) 1120
CviAII CATG 8 cut(s) 286, 389, 704, 803, 812, 911, 983, 1141
CviQI GTAC 1 cut(s) 1120
DdeI CTNAG 3 cut(s) 564, 969, 1002
DpnI GATC 7 cut(s) 122, 257, 366, 631, 770, 885, 953
DpnII GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
EaeI YGGCCR 1 cut(s) 94
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
EciI GGCGGA 2 cut(s) 227, 264
Ecl136II GAGCTC 1 cut(s) 297
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 108, 506, 703, 1140
Eco24I GRGCYC 2 cut(s) 105, 299
Eco31I GGTCTC 1 cut(s) 1136
Eco47I GGWCC 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 2 cut(s) 942, 945
EcoICRI GAGCTC 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 889
EcoT14I CCWWGG 4 cut(s) 108, 506, 703, 1140
EcoT38I GRGCYC 2 cut(s) 105, 299
ErhI CCWWGG 4 cut(s) 108, 506, 703, 1140
FaeI CATG 8 cut(s) 289, 392, 707, 806, 815, 914, 986, 1144
FaqI GGGAC 1 cut(s) 193
FatI CATG 8 cut(s) 285, 388, 703, 802, 811, 910, 982, 1140
FauI CCCGC 2 cut(s) 92, 127
FbaI TGATCA 2 cut(s) 120, 951
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 9 cut(s) 26, 36, 39, 97, 210, 247, 576, 860, 863
FokI GGATG 5 cut(s) 359, 524, 703, 1069, 1171
FriOI GRGCYC 2 cut(s) 105, 299
Fsp4HI GCNGC 9 cut(s) 26, 36, 39, 97, 210, 247, 576, 860, 863
FspBI CTAG 2 cut(s) 873, 1188
GlaI GCGC 2 cut(s) 387, 852
GluI GCNGC 9 cut(s) 26, 36, 39, 97, 210, 247, 576, 860, 863
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 3 cut(s) 96, 103, 157
HapII CCGG 5 cut(s) 66, 85, 305, 483, 1014
HgaI GACGC 4 cut(s) 9, 56, 71, 568
HhaI GCGC 2 cut(s) 388, 853
Hin1I GRCGYC 3 cut(s) 48, 63, 560
Hin1II CATG 8 cut(s) 289, 392, 707, 806, 815, 914, 986, 1144
Hin6I GCGC 2 cut(s) 386, 851
HinP1I GCGC 2 cut(s) 386, 851
HincII GTYRAC 3 cut(s) 46, 583, 719
HindII GTYRAC 3 cut(s) 46, 583, 719
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 2 cut(s) 146, 907
HpaII CCGG 5 cut(s) 66, 85, 305, 483, 1014
HphI GGTGA 3 cut(s) 196, 319, 1070
Hpy166II GTNNAC 4 cut(s) 46, 503, 583, 719
Hpy188I TCNGA 5 cut(s) 128, 294, 319, 964, 1172
Hpy188III TCNNGA 6 cut(s) 118, 143, 614, 904, 911, 1150
Hpy8I GTNNAC 4 cut(s) 46, 503, 583, 719
Hpy99I CGWCG 4 cut(s) 25, 50, 65, 200
HpyAV CCTTC 5 cut(s) 337, 544, 636, 707, 1147
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 2 cut(s) 524, 1034
HpyCH4V TGCA 7 cut(s) 350, 426, 694, 839, 862, 1073, 1196
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 347, 419, 859, 965, 1079, 1193
HpyF3I CTNAG 3 cut(s) 564, 969, 1002
HpySE526I ACGT 2 cut(s) 524, 1034
Hsp92I GRCGYC 3 cut(s) 48, 63, 560
Hsp92II CATG 8 cut(s) 289, 392, 707, 806, 815, 914, 986, 1144
HspAI GCGC 2 cut(s) 386, 851
Ksp22I TGATCA 2 cut(s) 120, 951
KspI CCGCGG 2 cut(s) 29, 100
Kzo9I GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
LmnI GCTCC 3 cut(s) 294, 302, 410
Lsp1109I GCAGC 3 cut(s) 587, 846, 874
LweI GCATC 5 cut(s) 337, 681, 907, 1082, 1091
MaeI CTAG 2 cut(s) 873, 1188
MaeII ACGT 2 cut(s) 524, 1034
MaeIII GTNAC 4 cut(s) 184, 478, 1024, 1092
MalI GATC 7 cut(s) 122, 257, 366, 631, 770, 885, 953
MbiI CCGCTC 1 cut(s) 774
MboI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
MboII GAAGA 3 cut(s) 878, 935, 1185
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 2 cut(s) 629, 768
MhlI GDGCHC 3 cut(s) 105, 299, 447
MluCI AATT 6 cut(s) 75, 351, 453, 648, 759, 994
MlyI GAGTC 1 cut(s) 916
MmeI TCCRAC 3 cut(s) 272, 607, 657
MseI TTAA 3 cut(s) 942, 1065, 1178
MspA1I CMGCKG 3 cut(s) 28, 99, 578
MspI CCGG 5 cut(s) 66, 85, 305, 483, 1014
MspR9I CCNGG 2 cut(s) 891, 1015
MunI CAATTG 1 cut(s) 351
Mva1269I GAATGC 4 cut(s) 37, 400, 730, 1145
MvaI CCWGG 1 cut(s) 891
MvnI CGCG 3 cut(s) 20, 28, 99
MwoI GCNNNNNNNGC 7 cut(s) 47, 347, 419, 859, 965, 1079, 1193
NciI CCSGG 1 cut(s) 1015
NcoI CCATGG 2 cut(s) 703, 1140
NdeII GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
NlaIII CATG 8 cut(s) 289, 392, 707, 806, 815, 914, 986, 1144
NlaIV GGNNCC 5 cut(s) 70, 103, 156, 444, 631
NmuCI GTSAC 2 cut(s) 184, 478
NspI RCATGY 1 cut(s) 392
PagI TCATGA 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 4 cut(s) 37, 400, 730, 1145
PfeI GAWTC 1 cut(s) 146
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 9 cut(s) 27, 37, 40, 98, 211, 248, 577, 861, 864
PleI GAGTC 1 cut(s) 915
PpsI GAGTC 1 cut(s) 915
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 299
Psp1406I AACGTT 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 889
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 5 cut(s) 70, 103, 156, 444, 631
PspOMI GGGCCC 1 cut(s) 101
PspPI GGNCC 4 cut(s) 101, 102, 112, 155
PspPPI RGGWCCY 1 cut(s) 112
PstI CTGCAG 1 cut(s) 864
PstNI CAGNNNCTG 3 cut(s) 172, 686, 859
PsuI RGATCY 2 cut(s) 629, 768
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 1121
RsaNI GTAC 1 cut(s) 1120
SacI GAGCTC 1 cut(s) 299
SacII CCGCGG 2 cut(s) 29, 100
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 3 cut(s) 942, 1065, 1178
SatI GCNGC 9 cut(s) 26, 36, 39, 97, 210, 247, 576, 860, 863
Sau3AI GATC 7 cut(s) 120, 255, 364, 629, 768, 883, 951
Sau96I GGNCC 4 cut(s) 101, 102, 112, 155
SchI GAGTC 1 cut(s) 916
ScrFI CCNGG 2 cut(s) 891, 1015
SduI GDGCHC 3 cut(s) 105, 299, 447
SfaNI GCATC 5 cut(s) 337, 681, 907, 1082, 1091
SfcI CTRYAG 1 cut(s) 860
Sfr303I CCGCGG 2 cut(s) 29, 100
SgrBI CCGCGG 2 cut(s) 29, 100
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 6 cut(s) 75, 351, 453, 648, 759, 994
SspMI CTAG 2 cut(s) 873, 1188
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 889, 1013
StyI CCWWGG 4 cut(s) 108, 506, 703, 1140
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 2 cut(s) 527, 1037
TaqI TCGA 5 cut(s) 45, 258, 495, 513, 1149
TasI AATT 6 cut(s) 75, 351, 453, 648, 759, 994
TatI WGTACW 1 cut(s) 1119
TauI GCSGC 6 cut(s) 28, 38, 41, 99, 212, 249
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 3 cut(s) 942, 1065, 1178
Tru9I TTAA 3 cut(s) 942, 1065, 1178
TseFI GTSAC 2 cut(s) 184, 478
TseI GCWGC 3 cut(s) 575, 859, 862
Tsp45I GTSAC 2 cut(s) 184, 478
TspDTI ATGAA 1 cut(s) 791
TspGWI ACGGA 2 cut(s) 289, 530
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 3 cut(s) 75, 453, 994
XceI RCATGY 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 2 cut(s) 873, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.