FvH4_7g32380

Secretory pathway protein Sec39

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004767
Physical Location & Seq
Forward (+)
23281927 .. 23293033
11107 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32380.t8

Sequence Viewer

Length: 7224 bp
ATGGATGAAACGGTAGACAGAGTGTTTTTCGAGACGAAAAGGCACATTACTAGACCGTGTACTCCCAATTATCCTCCTCAACAGGCAAATGATGGTGGTAAAGGGAACTTTCGATCACTGCTTTCCTTACAAGGTATCAGTCAACTCAGAGAAAAATGGAACGGATACAAGCAACCAAAAAAACCTAGAAGATTGACATCCTTATTAATCTCTCCAAGAGGTGAGTATGTAGCTGTGGTTGCTGGAAATCAGATAACAATTTTGAAGAAGGAGGATGACTACTCAGAGCCATGTGGAACTTTTACAAGTGGCAGCCCTGCTTCATTTACAACAGGAACCTGGTCAGAAAGTCATGATGTTCTTGGTGTCTCTGATGAGACGGATACATTATATTTTATCAAAGCCAATGGTTCAGAGATAGTAAGAATCAACAGGAGGCAATTAAAAGTATCTCTACCAGTGATAAGCTTGATTGTGCACGATGATTCTGATGTACAGAAATCTTGTTTGTGTAGCTTCATTATTGTTACGTCTGATGGTTCCCTCCAGCATATTGAGATTAGTCAGGATCCAAGTTCCTCTATCTATTCTGCACAGACATCACATAATGGACTAACTGTAAAGGGACAGTCCACTCATATTGTTTTGTGTGTTGACTACCATCCAGAGCTTTCATTGCTTGCTGGTGTCACGATAAAATCAGGGTCTTGTTATATTTCTGTTTGGCGTAGAAGTGGGACGATTGATCTGGAGCAGTTGTTTAGTGTTCAGTTTGATGGTTTTTATTCCCAACCAATAGGTTCTGGAAATCAGTTAGCATATCCAAAGGTTCTAATCTCACCACAAGCCAAATTTTTGGCTACTTTAGATCTGACAGGAAGGTTGCACGTATATAAGATGGATAAAGAAAGCTTCTCACTTTCAAGTTTTACATGCAGACAGAGATTTAAGTCACAAGTGACCAGTAATTTGTCAACTGCAGAACAGAAAGAGTTAATTGATATTGTGGACTTTACATGGTGGTCAGACCACATCCTTACTTTTGCAAAGCGGAGTGGCCTTGTGACCATGCTTGACCTTCTTAGTGGGTTAGAAATACAGGAAGATGGTTCTATATATTCTAAGCCTGTTTTAGAAAGAATAAAGTTGTTTCAAGGGAATCTGTTTCTTTTGGAGACTCTGTCTTCTGACGAGAGGTCTAATTCAGGTGAAACTAAAGATTCACATACGATGGAGCAAATAACTATGGACAGCCTTGACCAAATTGACATTTCCAGGTTGAACTGGAGCCTTGTATCATTTTCTGAGAGATCCGCTGTTGAAATGTATAATATTCTACTTAGAGATAAAAAGAATCAAGCTGCCTTGAAATTTGCTGATCATCATGGGCTGGACAAAGATGAAGTTATGAAATCACAATGGTTACACTCCAACCAAGGTGCAAATGACATACGTACATTGTTGTCAAAGGTGAAGGACAAACATTTTGTACTCTCTGAATGTGTTGAAAAGGTTGGACCAACTGAAGATTCTGTGAGGGCTATGCTTGTACATGGGCTGCACATAACTAACCAGTATGGGTTTTCTGAACCAGAAAATGATGAAGGTAGCCAAATTTGGGATTTCCGAATGGCTAGACTTAAGTTATTGCAATATAGTGACAGGTTGGAGACGTATCTCGGGATAAATATGGGCAGGTTTTCTGTGCAGGAATACAGCAAATTTCGAGCCATGCCTATAAATGAAGCTGCCGTAACACTTGCTGAAAGTGGGAAGATCGGAGCATTAAATCTTCTGTTCAAGCGTCATCCTTATTCCTTGGCTCCTTATGTATTGGAGATTTTAGGTTCTATCCCCGAAACAATTCCTGTGCAAACATATGGGCAACTTCTTCCTGGGAGGTCCCCGCCTACAAATATAGCTATGAGGGATAAGGATTGGGTTGAATGTGAGAAGATGATAAGTTTTATAAACAAGACACCCAAGGATCATGAGATTAGTATCCAAATCCAGACTGAACCTATAGTCAAGCAGTGCCGAGGGTCGGTTTGGCCATCCATTAAGGAAATAGCAATGTGGTACAAGAAGAGAGCTAGGGATATTGATACTTTTAGTGGGCAGCTAGAAAATTGTATGTCTTTGCTTGAGTTCGCTCATCACAAAGGTGTTCATGAGTTGCAGCAGTTCCATGATGATGTCTCTTACCTGCACCGACTGATTTATTCTGATGACAGTGGTGGTGAAGTAAATTCCAGTTTGACTCTTGTCATGTGGGAAGAATTATCTGATTATGATAAATTTAAAATGATGCTTAAAGGAGTAAAAGAAGAGAATCTGGTGGCAAGATTACATGATATGGCAGTTCCATTGATGCAAGATAGGTTTCATTATTCAACCTCTGTTTCTGATGGTTGGTTGACAGATGACCATCATGCTGCAGATGGTAATAAAGACGAGTCATTTCTGGTTCGCTGGCTGAAGGAAGCTGCCTATGAGAACAAATTGGATATATGCTTGTTGGTAATAGAGGAAGGGTGTAAAGATTTCCAAAGTAACAGCCTATTCAAGGATGAGGTTGAAGCTATAGATTGTGCCCTGCAGTGCATCTATCTGTGCACATCTACGGATAAGTGGAGTACTATGGCAGCTATATTGTCAAAACTTCCTCAAATGCAAGGTAGTGAAAGTTTTATTGAGAGTCTGGAGAGAAGATTGAAGCTGGCGGAAGGCCATATTGATGTCGGTAGACTCCTGGCATTTTACCAGGTGCCAAAGTTGCTGAACTTTTTCTTAGAGTCTCATGCAGATGGAAAAGGTGTAAAGCAAATTCTTCGCCTTATAATCTCAAAATTTATACGTCGGCAGCCTGGTCGATCAGATACTGATTGGGCAACCATGTGGCGGGATATGCAATGCATAAGGGAAAAAGCATTTCCTTTTCTGGACTTGGAGTATATGTTAATGGAATTTTGCAGAGGACTGCTTAAAGCTGGGAAATTTTCTCTTGCTAGGAACTATTTAAAGGGTACAAGTTCAGTTGCTTTGGCATCAGAGAAGGCTGAGAACCTTGTCATTCAAGCCGCAAGGGAGTATTTTTTCTCAGCTTCAAGCCTTTCTTGTCCTGAAATCTGGAAGGCTAAGGAATGCCTCAATTTATTTCCAAGCAGTGGAAACGTTAAAGTAGAGTCTGATATTATTGATGCACTTACAGTTAGACTTCCCAGTCTAGGAGTGACTCTGTTACCCGTGCAATTCAGGCAAATAAAAGATCCAATGGAGATTATCAAAATGGCAATCACAAGTGAAACAGGTGCATATTTACATGTTGATGAACTCATTGAGATTGCCAAACTTCTTGGATTGAGCTCTCCAGACAATATATCTTCTGTTCAGGAAGCTATTGCCAGAGAGGCTGCGGTTGCTGGTGATCTACAATTGGCTCTCGATCTCTGTCTTGTTTTGGCTAAGAAAGGTCATGGTCACATTTGGGATTTATCTGCTGCTATAGCAAGGGGTCCTGCACTTGAAAACATGGATATAAATTCTCGAAAGCAGCTGCTGGGTTTTGCTATAAGTAATTGTGACGAGGAATCTGTTAGTGAGTTGCTCCACGCTTGGAAAGATCTTGATTTGCAAGGTCAGTGTGAAACATTAATGATGTTGAGCGAGACAAAATGTCCAGATTACTCAATACATGGCTCCTCGATCATTACAGATTCAGTACACAATGTACAAGATATAATCAAGCTAAAAGGTTGCTTGGACATGGTTGAGGGAGCTAGTTCTGATGACCAGGAAGTTCATATTAGTAACATTAAGAATTCACTTTCTGCTGTTACTAAAAACTTGCCTGTTGATAATGGTACCGATTTGGAATCAATTTTGAGAGAAAATGGAAAATTTTTGTCTTTTGCTGCTATACAGTTTCCTTGGTTGCTTGGACTAAGTAGGAAAACAGAACACTGTAAGAAGCGAAATAGTAATGCACTTCCTGGAAAGCAGTTTGTCAGTGTTAGAACACAGGCTTTAGTAACCATTCTCTCCTGGTTGGCAAGGCATGGTTTAGCTCCTACAGACGATGTGGTTGCCTCTCTGGCAAAATCTATTATTGAACCACCTGTTACTGAAGAGGAATATACAGCCAGCTGCTCCTTTCTCTTGAATCTGGTGGATCCTTTTAATGGGGTTGAAGTGATAGAGGAGCAGCTTAGAACAAGGAAGGATTACCAAGAAATTTCTAGCATCATGAATGTGGGGATGACATATAGCTTATTGTTTAGTTCTGCAATCGAGTGTGAAAGTCCTATGCAAAGGAGGGAGCTACTATTGAGGAAGTTTAAAGAGAAGCACACACAACCTAGTGCTGATGAATTTGACAAATTTGACAAGGTAAAGTCTACCTTTTGGAGAGAATGGAAGCTGAAATTAGAAGACCAAAAGCGTGTTACAGATCACTGTAGAGCATTAGAGAAAATAATTCCTGGAGTTGACACAGCGCGCTTTTTGTCTCGAGACTCTAATTACATTGGGAGTGTTGTTCTTCCCCTTATTGATTCCGTTAAATTAGAGAAAAAACACATTTTAAAGGACATTCTGAAATTAGCTGATGGCTATGGCTTGAATCGTGCAGAGGTCCTTTTACGGTATCTAAGTTCTGTCCTTGTCTCTGAGGTATGGACAAATGATGATATCACAGCCGAAATATCAGAATTTAAAGGAGAAATAGTTCACCAAGCTGTTGAAACCATCAAAGCGATTTCCTCTGCTGTGTATCCTGCAGTCGATGGATGCAACAAGTTGCGCCTTGGTTACATGTTTGGTCTTCTTTCTGACTGCTACTTGCAACTGGAAGAAACTAGTAGAGAGTTACCAATATTACACCCTGATCAAGCGCATCTCTCTGGTTTTGGATTATCTCGTTTTTACAGGTTGGTGGAGCAAGAATGCGTAAGAGTTGCTTTTATTGTGGACCTGAACTTTAAAAAAATTGCTGGATTGGGGGGTTTGAACTTTAAGTGCTTAAGCTCTGAAGTCTACATGCATGTCAATGATAGTAGCTTGGAAGCTTTGTCAAAGATGATTCAGACTCTTACCAGCATATATTCCGATCCATTGCCAGAGGGTCTCATAACATGGCAGGATGTCTACAAACATTACATATGGAGTTTGCTGACAGCTTTAGAGACTAAAGCTGGAACAGCTTCAATCATCAAAAGCACTGAAACCCTTCAAGGTTTTGTGTGTCAACTTGAGCAGAGCTATGAATATTGCAGAAGGCATATCAGACTCTTGGCACATGTAGACTCTTTGAACATTATGAAGCGGTATTTCACAATAATCCTACCTCTTTTTGGTTCTTACGGGGGTCTACCAGATAACTCGGCATTGCAAGAGTGTCTTATAATTCTCTTGAACTTTTGGATAAGATTGATTGATGAAATGAAGGAAATTGCATCCCATGAGGATGCTGGAACAAATCTTAAGCTCAATTTAGATTGCTTATTGCAATGTCTAAAAGTTTGTATGAGGTTGGTAATGGAGGACAGTGTCTCCCCTAGTCAGGGGTGGGGTACACTCGTCAGCTTTGTCAAGCATGGTTTAATTGGTGATTCTGCTTCTGAACTTTATTTATTCTGTAGAGCGATGATTTTTTCTGGTTGTGGATTTGGACCTGTGGCTGAAGTATTTTCTGAAGCAGTAATTCGGGGTCCAACTGGTTTCACATTGGTGGGGGACAGAGAAATCCAGGAACTCCCTCATCTTTATTTGAATATCTTGGAACACATTTTACAGGATGTGGTGGTCAGTGAATCCCAGGAATACGAGAATCTATACCAGCTTTTATCCTCTTTGAGTAAATTAGAAGGAGATTTGGATGATTTGGATAGGGTCAGGAATATAATTTGGGAGAGAATGGCTGAGTTCTCTGATAACTTGCAGCTTCCCGGTTCCACTCGAGTTTATGCCTTAGAACTGATGCAATACCTGACAGGTAAAAACTCAAAGGGTTTCTCTGCTGCGATTCAATCTAATATTATACCATGGGAAGGATGGGATGAGATGCGCTTAACAAATAAAAAAAGTGAGACTACTGCCAATGAGGGTTTGGCAGATAATAGTGATAAATCTAACAGGTTTACAAGTACATTAGTTGCCCTCAAATCTTCTCAGCTTGTGGCAAACATCTCACCTACTATGGAAATTACCCCAGATGATATCCAGAATTTGGAGACAGCTGTTTCTTGCTTCCAAAAAATGTGTGATGTTGCTCAAAATTATTCACATGTTGAATCGTTGCTTGCTGTGTTGGGAGAATGGGAAGGATTTTTCTTGGTGAGGGAGGATAAGGAAGCCTCTGTACAAGTATCTGATGCTGGAAATGAATGGACTGGAGATAACTGGGATGAAGGATGGGAGAGTTTCCAAGAGAGTTCTATTTCCATTAACCCATTGCATGTGTGTTGGTTGGCAATCTTCAAGAAACTCGTAATGCTTTCCCATTTCAAAGATGTCCTTAGACTGATTGACCAGTCCTTACTGAAAGATAGTGGGATATTGCTTGATGAAGAAGGTGCAAGGAGTTTGAGCCAGATCTTTCTTGAAATAGATTGCTTCATGGCTTTAAAGTTGGTGCTATTACTGCCTTTTAAACCACTACAAGAACAGTGTTTGGCTGCTGTTGAGGACAAGTTGAAGCAAGCAGGCATCTCTGACACAATGGGTGGAGATCTTGAGTTATTGATGCTCGTGTTATTCTCTGGTGTTCTGTCCTCTATCATCTCCGATTCTTCATATGGTAATATGTTCTCTTATATCTGCTATTTGGTTGGGAATTTATCCCACAAGTGTCAAGCAGCTCAGTTACAAAATCAGAGGCGGAAAGGAAACAGTGCATTGGGAGAAAATGAGAGAGCCTTGTTGCTCTTTCGAACCGTACTATTCCCCTGTTTCATTTCAGAGCTTGTGAAGGGAGATCAGCAGCTTCTAGCTGGACTTGTTGTTACTAAATTCATGCACACAAACGCATCGCTTAGCCTTGTTAATATTGCTGAGGCAAGTCTCGGAAGATTTCTGGAGGTGCAGCTCAATGGTCTGCATGACAATTTCAATCTAGATGAGACACATTCACAGGATGCATTGCAGAATACTATATCCAGTTTGAGAGACAAAATGGAAAATCTGATCCAAGATGCATTGTCAACACTTTCAACTAACGTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006508 GO:0006624 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016485 GO:0019538 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec39

Protein Analysis

2408

Amino Acids

270.74

Weight (kDa)

5.33

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_KNTC1_1st PF24520 446 - 662 4.8e-09 KNTC1 first ARM-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1970, 2840, 5402
AasI GACNNNNNNGTC 1 cut(s) 3222
Acc36I ACCTGC 2 cut(s) 1686, 2214
Acc65I GGTACC 1 cut(s) 3863
AccB1I GGYRCC 2 cut(s) 2767, 3863
AccB7I CCANNNNNTGG 2 cut(s) 3167, 6226
AccI GTMKAC 7 cut(s) 15, 2746, 4397, 5034, 5145, 5301, 5368
AccII CGCG 1 cut(s) 4498
AciI CCGC 9 cut(s) 1051, 1314, 1907, 2723, 2902, 3081, 3416, 5323, 6877
AclI AACGTT 2 cut(s) 3174, 7215
AclWI GGATC 9 cut(s) 563, 576, 1305, 1995, 3263, 4166, 4179, 5102, 7177
AcoI YGGCCR 1 cut(s) 2051
AcuI CTGAAG 6 cut(s) 1545, 2498, 4146, 5049, 5700, 5712
AflII CTTAAG 3 cut(s) 1640, 5020, 5480
AflIII ACRYGT 4 cut(s) 3322, 4812, 5296, 6283
AhdI GACNNNNNGTC 2 cut(s) 1195, 3675
AhlI ACTAGT 1 cut(s) 4856
AjuI GAANNNNNNNTTGG 2 cut(s) 1513, 1545
AleI CACNNNNGTG 2 cut(s) 2163, 5726
AloI GAACNNNNNNTCC 2 cut(s) 4710, 4742
Alw21I GWGCWC 3 cut(s) 480, 2618, 3368
Alw44I GTGCAC 2 cut(s) 476, 2614
AlwI GGATC 9 cut(s) 563, 576, 1305, 1995, 3263, 4166, 4179, 5102, 7177
AlwNI CAGNNNCTG 2 cut(s) 2882, 3559
Ama87I CYCGRG 3 cut(s) 1679, 4509, 5955
AoxI GGCC 3 cut(s) 1057, 2051, 2728
ApaLI GTGCAC 2 cut(s) 476, 2614
ArsI GACNNNNNNTTYG 4 cut(s) 952, 984, 1469, 1501
AseI ATTAAT 2 cut(s) 206, 3653
Asp700I GAANNNNTTC 5 cut(s) 1863, 2786, 4725, 5200, 5226
Asp718I GGTACC 1 cut(s) 3863
AspLEI GCGC 5 cut(s) 4498, 4500, 4803, 4894, 6066
AspS9I GGNCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
AsuC2I CCSGG 1 cut(s) 5946
AsuII TTCGAA 1 cut(s) 6928
AvaI CYCGRG 3 cut(s) 1679, 4509, 5955
AvaII GGWCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
BaeGI GKGCMC 3 cut(s) 480, 2596, 2618
BaeI ACNNNNGTAYC 2 cut(s) 5562, 5595
BalI TGGCCA 1 cut(s) 2053
BamHI GGATCC 2 cut(s) 568, 4171
BanI GGYRCC 2 cut(s) 2767, 3863
BanII GRGCYC 1 cut(s) 3368
BarI GAAGNNNNNNTAC 2 cut(s) 6342, 6374
BauI CACGAG 1 cut(s) 6746
BbsI GAAGAC 3 cut(s) 1176, 4437, 4814
Bbv12I GWGCWC 3 cut(s) 480, 2618, 3368
BbvCI CCTCAGC 1 cut(s) 7050
BceAI ACGGC 1 cut(s) 1736
BcgI CGANNNNNNTGC 6 cut(s) 2813, 2847, 2852, 2886, 4067, 4101
BciVI GTATCC 4 cut(s) 158, 376, 2012, 4782
BclI TGATCA 2 cut(s) 1378, 4885
BcnI CCSGG 1 cut(s) 5946
BcuI ACTAGT 1 cut(s) 4856
BfrI CTTAAG 3 cut(s) 1640, 5020, 5480
BfuAI ACCTGC 2 cut(s) 1686, 2214
BfuI GTATCC 4 cut(s) 158, 376, 2012, 4782
BglI GCCNNNNNGGC 2 cut(s) 3410, 4094
BglII AGATCT 4 cut(s) 868, 3622, 6591, 6727
BlpI GCTNAGC 1 cut(s) 7031
BmcAI AGTACT 1 cut(s) 2638
Bme18I GGWCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
BmeRI GACNNNNNGTC 2 cut(s) 1195, 3675
BmeT110I CYCGRG 3 cut(s) 1679, 4509, 5955
BmgT120I GGNCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
BmrI ACTGGG 2 cut(s) 3216, 6409
BmuI ACTGGG 2 cut(s) 3216, 6409
BoxI GACNNNNGTC 1 cut(s) 2264
BpiI GAAGAC 3 cut(s) 1176, 4437, 4814
BpmI CTGGAG 8 cut(s) 530, 770, 1306, 2723, 3354, 4503, 6411, 7094
Bpu10I CCTNAGC 2 cut(s) 3138, 7050
Bpu1102I GCTNAGC 1 cut(s) 7031
Bpu14I TTCGAA 1 cut(s) 6928
BpuEI CTTGAG 3 cut(s) 2165, 5270, 6752
BpuMI CCSGG 1 cut(s) 5946
BsaAI YACGTR 2 cut(s) 889, 1454
BsaBI GATNNNNATC 3 cut(s) 196, 2000, 2427
BsaI GGTCTC 1 cut(s) 5129
BsaJI CCNNGG 9 cut(s) 1435, 1818, 1895, 1983, 2038, 3929, 4804, 5814, 6041
Bse3DI GCAATG 8 cut(s) 674, 2079, 2918, 5111, 5384, 5513, 6449, 7136
Bse8I GATNNNNATC 3 cut(s) 196, 2000, 2427
BseDI CCNNGG 9 cut(s) 1435, 1818, 1895, 1983, 2038, 3929, 4804, 5814, 6041
BseJI GATNNNNATC 3 cut(s) 196, 2000, 2427
BseMI GCAATG 8 cut(s) 674, 2079, 2918, 5111, 5384, 5513, 6449, 7136
BsePI GCGCGC 1 cut(s) 4496
BseRI GAGGAG 3 cut(s) 66, 3691, 4214
BseSI GKGCMC 3 cut(s) 480, 2596, 2618
BseYI CCCAGC 2 cut(s) 2990, 3559
BsgI GTGCAG 7 cut(s) 576, 1544, 1727, 2192, 3504, 4647, 7100
Bsh1236I CGCG 1 cut(s) 4498
BshFI GGCC 3 cut(s) 1059, 2053, 2730
BshNI GGYRCC 2 cut(s) 2767, 3863
BsiHKAI GWGCWC 3 cut(s) 480, 2618, 3368
BsiHKCI CYCGRG 3 cut(s) 1679, 4509, 5955
BsiSI CCGG 1 cut(s) 5946
BslFI GGGAC 4 cut(s) 639, 751, 1888, 5747
BsmBI CGTCTC 3 cut(s) 26, 371, 1664
BsmFI GGGAC 4 cut(s) 639, 751, 1888, 5747
BsmI GAATGC 2 cut(s) 3149, 4949
BsnI GGCC 3 cut(s) 1059, 2053, 2730
Bso31I GGTCTC 1 cut(s) 5129
BsoBI CYCGRG 3 cut(s) 1679, 4509, 5955
Bsp119I TTCGAA 1 cut(s) 6928
Bsp1286I GDGCHC 4 cut(s) 480, 2596, 2618, 3368
Bsp1407I TGTACA 4 cut(s) 493, 1549, 3730, 6358
Bsp1720I GCTNAGC 1 cut(s) 7031
Bsp19I CCATGG 1 cut(s) 6041
BspACI CCGC 9 cut(s) 1051, 1314, 1907, 2723, 2902, 3081, 3416, 5323, 6877
BspANI GGCC 3 cut(s) 1059, 2053, 2730
BspFNI CGCG 1 cut(s) 4498
BspHI TCATGA 4 cut(s) 352, 1990, 2170, 4245
BspMAI CTGCAG 4 cut(s) 982, 2440, 2601, 4780
BspMI ACCTGC 2 cut(s) 1686, 2214
BspPI GGATC 9 cut(s) 563, 576, 1305, 1995, 3263, 4166, 4179, 5102, 7177
BspT104I TTCGAA 1 cut(s) 6928
BspT107I GGYRCC 2 cut(s) 2767, 3863
BspTI CTTAAG 3 cut(s) 1640, 5020, 5480
BspTNI GGTCTC 1 cut(s) 5129
BsrDI GCAATG 8 cut(s) 674, 2079, 2918, 5111, 5384, 5513, 6449, 7136
BsrGI TGTACA 4 cut(s) 493, 1549, 3730, 6358
BssECI CCNNGG 9 cut(s) 1435, 1818, 1895, 1983, 2038, 3929, 4804, 5814, 6041
BssHII GCGCGC 1 cut(s) 4496
BssSI CACGAG 1 cut(s) 6746
BssT1I CCWWGG 6 cut(s) 1435, 1818, 1983, 3929, 4804, 6041
Bst2BI CACGAG 1 cut(s) 6746
Bst6I CTCTTC 3 cut(s) 2081, 2322, 4122
BstAFI CTTAAG 3 cut(s) 1640, 5020, 5480
BstAUI TGTACA 4 cut(s) 493, 1549, 3730, 6358
BstBAI YACGTR 2 cut(s) 889, 1454
BstBI TTCGAA 1 cut(s) 6928
BstC8I GCNNGC 8 cut(s) 681, 2474, 2721, 4144, 4498, 6300, 6699, 6703
BstDSI CCRYGG 1 cut(s) 6041
BstENI CCTNNNNNAGG 1 cut(s) 2564
BstFNI CGCG 1 cut(s) 4498
BstHHI GCGC 5 cut(s) 4498, 4500, 4803, 4894, 6066
BstNSI RCATGY 8 cut(s) 936, 3326, 4816, 5041, 5045, 5300, 6287, 6458
BstPAI GACNNNNGTC 1 cut(s) 2264
BstSLI GKGCMC 3 cut(s) 480, 2596, 2618
BstSNI TACGTA 1 cut(s) 1454
BstUI CGCG 1 cut(s) 4498
BstV2I GAAGAC 3 cut(s) 1176, 4437, 4814
BstX2I RGATCY 8 cut(s) 568, 868, 1310, 3268, 3622, 4171, 6591, 6727
BstXI CCANNNNNNTGG 1 cut(s) 856
BstYI RGATCY 8 cut(s) 568, 868, 1310, 3268, 3622, 4171, 6591, 6727
BsuI GTATCC 4 cut(s) 158, 376, 2012, 4782
BsuRI GGCC 3 cut(s) 1059, 2053, 2730
BtgI CCRYGG 1 cut(s) 6041
BtgZI GCGATG 2 cut(s) 5657, 7011
BtsI GCAGTG 4 cut(s) 116, 2039, 2606, 3172
BveI ACCTGC 2 cut(s) 1686, 2214
Cac8I GCNNGC 8 cut(s) 681, 2474, 2721, 4144, 4498, 6300, 6699, 6703
CaiI CAGNNNCTG 2 cut(s) 2882, 3559
CciI TCATGA 4 cut(s) 352, 1990, 2170, 4245
CfoI GCGC 5 cut(s) 4498, 4500, 4803, 4894, 6066
Cfr13I GGNCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
CseI GACGC 1 cut(s) 1793
CsiI ACCWGGT 2 cut(s) 338, 2763
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
DraI TTTAAA 8 cut(s) 2302, 3021, 4339, 4584, 4714, 4981, 6624, 6649
DrdI GACNNNNNNGTC 1 cut(s) 3222
DriI GACNNNNNGTC 2 cut(s) 1195, 3675
DseDI GACNNNNNNGTC 1 cut(s) 3222
EaeI YGGCCR 1 cut(s) 2051
Eam1104I CTCTTC 3 cut(s) 2081, 2322, 4122
Eam1105I GACNNNNNGTC 2 cut(s) 1195, 3675
EarI CTCTTC 3 cut(s) 2081, 2322, 4122
EciI GGCGGA 2 cut(s) 2738, 6892
Ecl136II GAGCTC 1 cut(s) 3366
Eco105I TACGTA 1 cut(s) 1454
Eco130I CCWWGG 6 cut(s) 1435, 1818, 1983, 3929, 4804, 6041
Eco24I GRGCYC 1 cut(s) 3368
Eco31I GGTCTC 1 cut(s) 5129
Eco32I GATATC 2 cut(s) 4690, 6217
Eco47I GGWCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
Eco53kI GAGCTC 1 cut(s) 3366
Eco57I CTGAAG 6 cut(s) 1545, 2498, 4146, 5049, 5700, 5712
Eco88I CYCGRG 3 cut(s) 1679, 4509, 5955
EcoICRI GAGCTC 1 cut(s) 3366
EcoNI CCTNNNNNAGG 1 cut(s) 2564
EcoO109I RGGNCCY 3 cut(s) 1902, 3515, 4633
EcoRI GAATTC 1 cut(s) 3820
EcoRV GATATC 2 cut(s) 4690, 6217
EcoT14I CCWWGG 6 cut(s) 1435, 1818, 1983, 3929, 4804, 6041
EcoT22I ATGCAT 4 cut(s) 2918, 5043, 7138, 7195
EcoT38I GRGCYC 1 cut(s) 3368
ErhI CCWWGG 6 cut(s) 1435, 1818, 1983, 3929, 4804, 6041
Esp3I CGTCTC 3 cut(s) 26, 371, 1664
FaqI GGGAC 4 cut(s) 639, 751, 1888, 5747
FauI CCCGC 2 cut(s) 1914, 2895
FauNDI CATATG 3 cut(s) 1879, 5159, 6793
FbaI TGATCA 2 cut(s) 1378, 4885
FblI GTMKAC 7 cut(s) 15, 2746, 4397, 5034, 5145, 5301, 5368
FriOI GRGCYC 1 cut(s) 3368
GlaI GCGC 5 cut(s) 4497, 4499, 4802, 4893, 6065
GsaI CCCAGC 2 cut(s) 2994, 3563
GsuI CTGGAG 8 cut(s) 530, 770, 1306, 2723, 3354, 4503, 6411, 7094
HaeIII GGCC 3 cut(s) 1059, 2053, 2730
HapII CCGG 1 cut(s) 5946
HgaI GACGC 1 cut(s) 1793
HhaI GCGC 5 cut(s) 4498, 4500, 4803, 4894, 6066
Hin6I GCGC 5 cut(s) 4496, 4498, 4801, 4892, 6064
HinP1I GCGC 5 cut(s) 4496, 4498, 4801, 4892, 6064
HincII GTYRAC 7 cut(s) 143, 655, 975, 2418, 4489, 5246, 7200
HindII GTYRAC 7 cut(s) 143, 655, 975, 2418, 4489, 5246, 7200
HindIII AAGCTT 3 cut(s) 466, 910, 5064
HpaII CCGG 1 cut(s) 5946
Hpy99I CGWCG 1 cut(s) 2862
HpyCH4IV ACGT 7 cut(s) 530, 888, 1453, 1673, 2857, 3174, 7215
HpySE526I ACGT 7 cut(s) 530, 888, 1453, 1673, 2857, 3174, 7215
HspAI GCGC 5 cut(s) 4496, 4498, 4801, 4892, 6064
KpnI GGTACC 1 cut(s) 3867
Ksp22I TGATCA 2 cut(s) 1378, 4885
MabI ACCWGGT 2 cut(s) 338, 2763
MaeII ACGT 7 cut(s) 530, 888, 1453, 1673, 2857, 3174, 7215
MfeI CAATTG 1 cut(s) 3434
MflI RGATCY 8 cut(s) 568, 868, 1310, 3268, 3622, 4171, 6591, 6727
MhlI GDGCHC 4 cut(s) 480, 2596, 2618, 3368
MlsI TGGCCA 1 cut(s) 2053
MluNI TGGCCA 1 cut(s) 2053
MmeI TCCRAC 4 cut(s) 1455, 1495, 1647, 5735
Mox20I TGGCCA 1 cut(s) 2053
Mph1103I ATGCAT 4 cut(s) 2918, 5043, 7138, 7195
MroXI GAANNNNTTC 5 cut(s) 1863, 2786, 4725, 5200, 5226
MscI TGGCCA 1 cut(s) 2053
MslI CAYNNNNRTG 9 cut(s) 2163, 2193, 2736, 2805, 3327, 4250, 5046, 5463, 5726
Msp20I TGGCCA 1 cut(s) 2053
MspA1I CMGCKG 4 cut(s) 1316, 3556, 4146, 6236
MspCI CTTAAG 3 cut(s) 1640, 5020, 5480
MspI CCGG 1 cut(s) 5946
MunI CAATTG 1 cut(s) 3434
Mva1269I GAATGC 2 cut(s) 3149, 4949
MvnI CGCG 1 cut(s) 4498
NciI CCSGG 1 cut(s) 5946
NcoI CCATGG 1 cut(s) 6041
NdeI CATATG 3 cut(s) 1879, 5159, 6793
NmeAIII GCCGAG 2 cut(s) 2063, 5360
NmuCI GTSAC 8 cut(s) 688, 951, 958, 1063, 1658, 3232, 3479, 3581
NsiI ATGCAT 4 cut(s) 2918, 5043, 7138, 7195
NspI RCATGY 8 cut(s) 936, 3326, 4816, 5041, 5045, 5300, 6287, 6458
NspV TTCGAA 1 cut(s) 6928
OliI CACNNNNGTG 2 cut(s) 2163, 5726
PaeR7I CTCGAG 2 cut(s) 4509, 5955
PagI TCATGA 4 cut(s) 352, 1990, 2170, 4245
PauI GCGCGC 1 cut(s) 4496
PciI ACATGT 4 cut(s) 3322, 4812, 5296, 6283
PctI GAATGC 2 cut(s) 3149, 4949
PdmI GAANNNNTTC 5 cut(s) 1863, 2786, 4725, 5200, 5226
PflFI GACNNNGTC 2 cut(s) 1180, 5546
PflMI CCANNNNNTGG 2 cut(s) 3167, 6226
PfoI TCCNGGA 3 cut(s) 3991, 4480, 5745
Ppu21I YACGTR 2 cut(s) 889, 1454
PpuMI RGGWCCY 3 cut(s) 1902, 3515, 4633
PscI ACATGT 4 cut(s) 3322, 4812, 5296, 6283
PshAI GACNNNNGTC 1 cut(s) 2264
PshBI ATTAAT 2 cut(s) 206, 3653
PsiI TTATAA 3 cut(s) 1970, 2840, 5402
Psp124BI GAGCTC 1 cut(s) 3368
Psp1406I AACGTT 2 cut(s) 3174, 7215
Psp5II RGGWCCY 3 cut(s) 1902, 3515, 4633
PspFI CCCAGC 2 cut(s) 2990, 3559
PspPI GGNCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
PspPPI RGGWCCY 3 cut(s) 1902, 3515, 4633
PspXI VCTCGAGB 1 cut(s) 5955
PsrI GAACNNNNNNTAC 2 cut(s) 5772, 5804
PstI CTGCAG 4 cut(s) 982, 2440, 2601, 4780
PstNI CAGNNNCTG 2 cut(s) 2882, 3559
PsuI RGATCY 8 cut(s) 568, 868, 1310, 3268, 3622, 4171, 6591, 6727
PsyI GACNNNGTC 2 cut(s) 1180, 5546
PteI GCGCGC 1 cut(s) 4496
PvuII CAGCTG 3 cut(s) 3556, 4146, 6236
RseI CAYNNNNRTG 9 cut(s) 2163, 2193, 2736, 2805, 3327, 4250, 5046, 5463, 5726
SacI GAGCTC 1 cut(s) 3368
Sau96I GGNCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
ScaI AGTACT 1 cut(s) 2638
SduI GDGCHC 4 cut(s) 480, 2596, 2618, 3368
SexAI ACCWGGT 2 cut(s) 338, 2763
Sfr274I CTCGAG 2 cut(s) 4509, 5955
SfuI TTCGAA 1 cut(s) 6928
SinI GGWCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
SlaI CTCGAG 2 cut(s) 4509, 5955
SmiMI CAYNNNNRTG 9 cut(s) 2163, 2193, 2736, 2805, 3327, 4250, 5046, 5463, 5726
SmlI CTYRAG 8 cut(s) 1640, 2144, 4509, 5020, 5249, 5480, 5955, 6731
SmoI CTYRAG 8 cut(s) 1640, 2144, 4509, 5020, 5249, 5480, 5955, 6731
SnaBI TACGTA 1 cut(s) 1454
SpeI ACTAGT 1 cut(s) 4856
SsiI CCGC 9 cut(s) 1051, 1314, 1907, 2723, 2902, 3081, 3416, 5323, 6877
SspI AATATT 5 cut(s) 1333, 4875, 5267, 6034, 7045
SstI GAGCTC 1 cut(s) 3368
StyI CCWWGG 6 cut(s) 1435, 1818, 1983, 3929, 4804, 6041
TaiI ACGT 7 cut(s) 533, 891, 1456, 1676, 2860, 3177, 7218
TatI WGTACW 9 cut(s) 59, 493, 1489, 1549, 2636, 3721, 3730, 6143, 6358
TauI GCSGC 1 cut(s) 3083
TseFI GTSAC 8 cut(s) 688, 951, 958, 1063, 1658, 3232, 3479, 3581
Tsp45I GTSAC 8 cut(s) 688, 951, 958, 1063, 1658, 3232, 3479, 3581
TspGWI ACGGA 4 cut(s) 177, 395, 2639, 4546
Tth111I GACNNNGTC 2 cut(s) 1180, 5546
Van91I CCANNNNNTGG 2 cut(s) 3167, 6226
Vha464I CTTAAG 3 cut(s) 1640, 5020, 5480
VneI GTGCAC 2 cut(s) 476, 2614
VpaK11BI GGWCC 7 cut(s) 1517, 1902, 3515, 4633, 4969, 5669, 5708
VspI ATTAAT 2 cut(s) 206, 3653
XagI CCTNNNNNAGG 1 cut(s) 2564
XbaI TCTAGA 1 cut(s) 7111
XceI RCATGY 8 cut(s) 936, 3326, 4816, 5041, 5045, 5300, 6287, 6458
XcmI CCANNNNNNNNNTGG 3 cut(s) 5465, 6048, 6103
XhoI CTCGAG 2 cut(s) 4509, 5955
XmiI GTMKAC 7 cut(s) 15, 2746, 4397, 5034, 5145, 5301, 5368
XmnI GAANNNNTTC 5 cut(s) 1863, 2786, 4725, 5200, 5226
ZrmI AGTACT 1 cut(s) 2638
Zsp2I ATGCAT 4 cut(s) 2918, 5043, 7138, 7195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.