FvH4_7g32530

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016511
Physical Location & Seq
Forward (+)
23379515 .. 23381376
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32530.t1

Sequence Viewer

Length: 402 bp
ATGGCTTCAGATCTGAAAGTTCACGTTTTTGAAGAGGTGGCTAAGCACAACCAGACCAAAGATTGCTGGCTTATTATTTCTGGGAAGGTGTATGATGTGACCCCTTTCATGGAAGATCATCCCGGAGGAGATGAAGTTTTGCTAGCTGCAACAGGGAAAGATGGAACAAATGATTTTGAAGATGTTGGTCACAGTGATTCTGCCAGAGAGATGATGGACAAGTACTACATTGGAGAGATCGATGCATCAACTGTCCCACTAAAACGGACCTTCGTTCCGCCACCGCAGACTTACAATCCGGATAAGACATCCGAGTTTGTGATCAAGATTTTACAGTTCCTGGTGCCCCTGTTAATCTTGGGTTTAGCCTTTGCCGTCCGTCACTTTACCAAGAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 OPT

Protein Analysis

134

Amino Acids

14.96

Weight (kDa)

5.15

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 11 - 80 5.2e-30 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53560
fragaria_vesca FvH4_7g32530
malus_domestica MD01G1226000.v1.1 MD07G1296500.v1.1
prunus_persica Prupe.2G316100_v2.0.a1 Prupe.2G316100_v2.0.a1
pyrus_communis pycom01g23460
rosa_chinensis RchiOBHm_Chr1g0382371
rosa_roxburghii Rroxscaffold_7G00167650
rosa_samantha Rh1AG458500 Rh1BG414800 Rh1CG428900 Rh1DG446900
rosa_wichuraiana Rw1G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 343
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 2 cut(s) 278, 284
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 109
AgsI TTSAA 2 cut(s) 32, 179
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 1 cut(s) 146
AluI AGCT 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 340
Aor13HI TCCGGA 1 cut(s) 298
ApeKI GCWGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 123
AsuNHI GCTAGC 1 cut(s) 142
AvaII GGWCC 1 cut(s) 267
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 343
BbvI GCAGC 1 cut(s) 133
BccI CCATC 2 cut(s) 155, 208
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 341
BclI TGATCA 1 cut(s) 321
BcnI CCSGG 1 cut(s) 123
BfaI CTAG 1 cut(s) 143
BglII AGATCT 1 cut(s) 10
BisI GCNGC 1 cut(s) 147
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 148
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 123, 341
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 2 cut(s) 123, 341
BmsI GCATC 2 cut(s) 232, 254
BmtI GCTAGC 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 123
Bsa29I ATCGAT 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 109
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 341
BseCI ATCGAT 1 cut(s) 240
BseGI GGATG 2 cut(s) 118, 308
BseLI CCNNNNNNNGG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 240
BsiSI CCGG 2 cut(s) 123, 299
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 348
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 4 cut(s) 10, 115, 237, 321
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 2 cut(s) 278, 284
BspDI ATCGAT 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 345
BspOI GCTAGC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 343
BssMI GATC 4 cut(s) 10, 115, 237, 321
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 3 cut(s) 194, 253, 336
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 68, 144
BstDEI CTNAG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 118, 308
BstKTI GATC 4 cut(s) 13, 118, 240, 324
BstMBI GATC 4 cut(s) 10, 115, 237, 321
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 2 cut(s) 121, 339
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
Bsu15I ATCGAT 1 cut(s) 240
BsuTUI ATCGAT 1 cut(s) 240
BtsCI GGATG 2 cut(s) 118, 308
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 68, 144
CaiI CAGNNNCTG 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 267
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 109
CviJI RGCY 5 cut(s) 5, 41, 70, 146, 368
CviKI_1 RGCY 5 cut(s) 5, 41, 70, 146, 368
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 42
DpnI GATC 4 cut(s) 12, 117, 239, 323
DpnII GATC 4 cut(s) 10, 115, 237, 321
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 247
FaeI CATG 1 cut(s) 112
FaiI YATR 2 cut(s) 93, 110
FaqI GGGAC 1 cut(s) 239
FatI CATG 1 cut(s) 108
FbaI TGATCA 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 2 cut(s) 105, 295
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 1 cut(s) 143
GluI GCNGC 1 cut(s) 147
HapII CCGG 2 cut(s) 123, 299
Hin1II CATG 1 cut(s) 112
HinfI GANTC 1 cut(s) 197
HpaII CCGG 2 cut(s) 123, 299
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 10, 15, 313
Hpy188III TCNNGA 2 cut(s) 299, 325
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 2 cut(s) 79, 280
HpyCH4III ACNGT 3 cut(s) 194, 253, 336
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 2 cut(s) 149, 245
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 321
Kzo9I GATC 4 cut(s) 10, 115, 237, 321
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 2 cut(s) 232, 254
MaeI CTAG 1 cut(s) 143
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 3 cut(s) 97, 188, 380
MalI GATC 4 cut(s) 12, 117, 239, 323
MboI GATC 4 cut(s) 10, 115, 237, 321
MboII GAAGA 3 cut(s) 44, 125, 191
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 1 cut(s) 348
MnlI CCTC 2 cut(s) 28, 119
Mph1103I ATGCAT 1 cut(s) 247
MroI TCCGGA 1 cut(s) 298
MseI TTAA 1 cut(s) 353
MspI CCGG 2 cut(s) 123, 299
MspR9I CCNGG 2 cut(s) 123, 341
MvaI CCWGG 1 cut(s) 341
NciI CCSGG 1 cut(s) 123
NdeII GATC 4 cut(s) 10, 115, 237, 321
NheI GCTAGC 1 cut(s) 142
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 345
NmuCI GTSAC 3 cut(s) 97, 188, 380
NsiI ATGCAT 1 cut(s) 247
PfeI GAWTC 1 cut(s) 197
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 4 cut(s) 10, 115, 237, 321
Sau96I GGNCC 1 cut(s) 267
ScaI AGTACT 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 123, 341
SduI GDGCHC 1 cut(s) 348
SetI ASST 5 cut(s) 27, 39, 90, 148, 272
SfaNI GCATC 2 cut(s) 232, 254
SinI GGWCC 1 cut(s) 267
SsiI CCGC 2 cut(s) 278, 284
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 121, 339
TaaI ACNGT 3 cut(s) 194, 253, 336
TaiI ACGT 1 cut(s) 27
TaqI TCGA 1 cut(s) 240
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 3 cut(s) 97, 188, 380
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 3 cut(s) 97, 188, 380
TspDTI ATGAA 2 cut(s) 97, 147
TspGWI ACGGA 2 cut(s) 280, 368
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 267
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XspI CTAG 1 cut(s) 143
ZrmI AGTACT 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.