RchiOBHm_Chr1g0382371

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016511
Physical Location & Seq
Forward (+)
67296796 .. 67298692
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60536

Sequence Viewer

Length: 405 bp
ATGGCTTCAGATCCGAAGGTTCACGTGTTTGAAGAGGTGGCCAAGCACAACCAGACCAAAGATTGCTGGCTTGTTATTTCTGGGAAGGTGTACGATGTAACCCCTTTCATGGATGATCATCCCGGAGGAGATGAAGTTCTGCTATCTGCAACAGGGAAAGACGCAACAAATGATTTTGAAGATGTTGGTCATAGTGATGCTGCCAGAGAAATGATGGACAAGTACTACATTGGAGAGATTGATCCATCAACTGTCCCATTAAAACGGACTTACATTCCGCCACCGCAGACTCAGTACAATCCGGATAAGACATCGGAGTTTGTGATCAAGATTTTACAGTTCCTGGTGCCCTTGTTAATCTTGGGTTTAGCCTTTGCTGTCCGTCATTTTACCAAGAAAGAGTAG

Protein Analysis

134

Amino Acids

15.11

Weight (kDa)

5.14

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 11 - 80 3.4e-31 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53560
fragaria_vesca FvH4_7g32530
malus_domestica MD01G1226000.v1.1 MD07G1296500.v1.1
prunus_persica Prupe.2G316100_v2.0.a1 Prupe.2G316100_v2.0.a1
pyrus_communis pycom01g23460
rosa_chinensis RchiOBHm_Chr1g0382371
rosa_roxburghii Rroxscaffold_7G00167650
rosa_samantha Rh1AG458500 Rh1BG414800 Rh1CG428900 Rh1DG446900
rosa_wichuraiana Rw1G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 2 cut(s) 278, 284
AclWI GGATC 2 cut(s) 5, 236
AcoI YGGCCR 1 cut(s) 39
AcvI CACGTG 1 cut(s) 25
AfaI GTAC 3 cut(s) 92, 224, 296
AfiI CCNNNNNNNGG 1 cut(s) 109
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 2 cut(s) 32, 179
AjnI CCWGG 1 cut(s) 342
AloI GAACNNNNNNTCC 2 cut(s) 120, 152
AlwI GGATC 2 cut(s) 5, 236
AlwNI CAGNNNCTG 1 cut(s) 343
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 351
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 346
BbrPI CACGTG 1 cut(s) 25
BbvI GCAGC 1 cut(s) 187
BccI CCATC 2 cut(s) 208, 253
BciT130I CCWGG 1 cut(s) 344
BclI TGATCA 2 cut(s) 115, 324
BcnI CCSGG 1 cut(s) 123
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 123, 344
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 2 cut(s) 123, 344
BmsI GCATC 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 25
BsaBI GATNNNNATC 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 118, 118
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 1 cut(s) 187
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 346
BsiSI CCGG 2 cut(s) 123, 302
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 351
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 4 cut(s) 10, 115, 241, 324
BspACI CCGC 2 cut(s) 278, 284
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 304
BspEI TCCGGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 2 cut(s) 5, 236
BspT107I GGYRCC 1 cut(s) 346
BssMI GATC 4 cut(s) 10, 115, 241, 324
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 253, 339
Bst6I CTCTTC 1 cut(s) 27
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 2 cut(s) 118, 118
BstKTI GATC 4 cut(s) 13, 118, 244, 327
BstMBI GATC 4 cut(s) 10, 115, 241, 324
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 2 cut(s) 121, 342
BstSLI GKGCMC 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 1 cut(s) 41
BtsCI GGATG 2 cut(s) 118, 118
Cac8I GCNNGC 1 cut(s) 68
CaiI CAGNNNCTG 1 cut(s) 343
CseI GACGC 1 cut(s) 170
Csp6I GTAC 3 cut(s) 91, 223, 295
CviAII CATG 1 cut(s) 109
CviJI RGCY 4 cut(s) 5, 41, 70, 371
CviKI_1 RGCY 4 cut(s) 5, 41, 70, 371
CviQI GTAC 3 cut(s) 91, 223, 295
DdeI CTNAG 1 cut(s) 291
DpnI GATC 4 cut(s) 12, 117, 243, 326
DpnII GATC 4 cut(s) 10, 115, 241, 324
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 267
Eco72I CACGTG 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 342
FaeI CATG 1 cut(s) 112
FaiI YATR 2 cut(s) 110, 192
FaqI GGGAC 1 cut(s) 239
FatI CATG 1 cut(s) 108
FbaI TGATCA 2 cut(s) 115, 324
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 2 cut(s) 105, 125
Fsp4HI GCNGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 123, 302
HgaI GACGC 1 cut(s) 170
Hin1II CATG 1 cut(s) 112
HinfI GANTC 1 cut(s) 289
HpaII CCGG 2 cut(s) 123, 302
Hpy166II GTNNAC 2 cut(s) 22, 91
Hpy188I TCNGA 3 cut(s) 10, 15, 316
Hpy188III TCNNGA 2 cut(s) 302, 328
Hpy8I GTNNAC 2 cut(s) 22, 91
HpyAV CCTTC 2 cut(s) 10, 79
HpyCH4III ACNGT 2 cut(s) 253, 339
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 1 cut(s) 149
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 301
Ksp22I TGATCA 2 cut(s) 115, 324
Kzo9I GATC 4 cut(s) 10, 115, 241, 324
LpnPI CCDG 9 cut(s) 52, 65, 66, 136, 138, 217, 315, 329, 356
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 187
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 97
MalI GATC 4 cut(s) 12, 117, 243, 326
MboI GATC 4 cut(s) 10, 115, 241, 324
MboII GAAGA 2 cut(s) 44, 191
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 41
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 283
MnlI CCTC 2 cut(s) 28, 119
Mox20I TGGCCA 1 cut(s) 41
MroI TCCGGA 1 cut(s) 301
MscI TGGCCA 1 cut(s) 41
MseI TTAA 2 cut(s) 260, 356
MslI CAYNNNNRTG 1 cut(s) 195
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 2 cut(s) 123, 302
MspR9I CCNGG 2 cut(s) 123, 344
MvaI CCWGG 1 cut(s) 344
NciI CCSGG 1 cut(s) 123
NdeII GATC 4 cut(s) 10, 115, 241, 324
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 348
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 283
PmaCI CACGTG 1 cut(s) 25
PmlI CACGTG 1 cut(s) 25
PpsI GAGTC 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 342
PspCI CACGTG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 343
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 3 cut(s) 92, 224, 296
RsaNI GTAC 3 cut(s) 91, 223, 295
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 2 cut(s) 260, 356
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 4 cut(s) 10, 115, 241, 324
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 123, 344
SduI GDGCHC 1 cut(s) 351
SetI ASST 4 cut(s) 21, 27, 39, 90
SfaNI GCATC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 195
SsiI CCGC 2 cut(s) 278, 284
StyD4I CCNGG 2 cut(s) 121, 342
TaaI ACNGT 2 cut(s) 253, 339
TaiI ACGT 1 cut(s) 27
TatI WGTACW 2 cut(s) 222, 294
Tru1I TTAA 2 cut(s) 260, 356
Tru9I TTAA 2 cut(s) 260, 356
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 97, 147
TspGWI ACGGA 2 cut(s) 280, 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.