Rw1G041250

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016511
Physical Location & Seq
Reverse (-)
68177847 .. 68179309
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G041250.1

Sequence Viewer

Length: 432 bp
ATGGTGTTTTTCTGTTTGGTTGCAGTAATGGCTTCAGATCCGAAGGTTCACGTGTTTGAAGAGGTGGCCAAGCACAACCAGACCAAAGATTGCTGGCTTGTTATTTCTGGGAAGGTGTACGATGTAACCCCTTTCATGGATGATCATCCCGGAGGAGATGAAGTTCTGCTATCTGCAACAGGGAAAGACGCAACAAATGATTTTGAAGATGTTGGTCATAGTGATGCTGCCAGAGATATGATGGACAAGTACTACATTGGAGAGATTGATCCGTCAACTGTCCCATTAAAACGGACTTACATTCCACCACCGCAGACTCAGTACAATCCGGATAAGACATCGGAGTTTGTGATCAAGATTTTACAGTTCCTGGTGCCCTTGTTAATCTTGGGTTTAGCCTTTGCTGTCCGTCATTTTACCAAGAAAGAGTAG

Protein Analysis

143

Amino Acids

16.11

Weight (kDa)

5.12

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 20 - 89 6.8e-31 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53560
fragaria_vesca FvH4_7g32530
malus_domestica MD01G1226000.v1.1 MD07G1296500.v1.1
prunus_persica Prupe.2G316100_v2.0.a1 Prupe.2G316100_v2.0.a1
pyrus_communis pycom01g23460
rosa_chinensis RchiOBHm_Chr1g0382371
rosa_roxburghii Rroxscaffold_7G00167650
rosa_samantha Rh1AG458500 Rh1BG414800 Rh1CG428900 Rh1DG446900
rosa_wichuraiana Rw1G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 1 cut(s) 311
AclWI GGATC 2 cut(s) 32, 263
AcoI YGGCCR 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 18
AcvI CACGTG 1 cut(s) 52
AfaI GTAC 3 cut(s) 119, 251, 323
AfiI CCNNNNNNNGG 1 cut(s) 136
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 2 cut(s) 59, 206
AjnI CCWGG 1 cut(s) 369
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AlwI GGATC 2 cut(s) 32, 263
AlwNI CAGNNNCTG 1 cut(s) 370
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 378
BalI TGGCCA 1 cut(s) 68
BanI GGYRCC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 52
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 2 cut(s) 142, 351
BcnI CCSGG 1 cut(s) 150
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 150, 371
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 2 cut(s) 150, 371
BmsI GCATC 1 cut(s) 214
BpuMI CCSGG 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 144
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 1 cut(s) 371
BseGI GGATG 2 cut(s) 145, 145
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 332
BseRI GAGGAG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 1 cut(s) 214
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 373
BsiSI CCGG 2 cut(s) 150, 329
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 378
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 4 cut(s) 37, 142, 268, 351
BspACI CCGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 331
BspEI TCCGGA 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 375
BspPI GGATC 2 cut(s) 32, 263
BspT107I GGYRCC 1 cut(s) 373
BssMI GATC 4 cut(s) 37, 142, 268, 351
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 280, 366
Bst6I CTCTTC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 2 cut(s) 145, 145
BstKTI GATC 4 cut(s) 40, 145, 271, 354
BstMBI GATC 4 cut(s) 37, 142, 268, 351
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 148, 369
BstSLI GKGCMC 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 2 cut(s) 145, 145
Cac8I GCNNGC 1 cut(s) 95
CaiI CAGNNNCTG 1 cut(s) 370
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 118, 250, 322
CviAII CATG 1 cut(s) 136
CviJI RGCY 4 cut(s) 32, 68, 97, 398
CviKI_1 RGCY 4 cut(s) 32, 68, 97, 398
CviQI GTAC 3 cut(s) 118, 250, 322
DdeI CTNAG 1 cut(s) 318
DpnI GATC 4 cut(s) 39, 144, 270, 353
DpnII GATC 4 cut(s) 37, 142, 268, 351
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 18
Eco72I CACGTG 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 369
FaeI CATG 1 cut(s) 139
FaiI YATR 3 cut(s) 137, 219, 239
FaqI GGGAC 1 cut(s) 266
FatI CATG 1 cut(s) 135
FbaI TGATCA 2 cut(s) 142, 351
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 2 cut(s) 132, 152
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 150, 329
HgaI GACGC 1 cut(s) 197
Hin1II CATG 1 cut(s) 139
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 1 cut(s) 316
HpaII CCGG 2 cut(s) 150, 329
Hpy166II GTNNAC 3 cut(s) 49, 118, 276
Hpy188I TCNGA 3 cut(s) 37, 42, 343
Hpy188III TCNNGA 2 cut(s) 329, 355
Hpy8I GTNNAC 3 cut(s) 49, 118, 276
HpyAV CCTTC 2 cut(s) 37, 106
HpyCH4III ACNGT 2 cut(s) 280, 366
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 23, 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 139
Kpn2I TCCGGA 1 cut(s) 328
Ksp22I TGATCA 2 cut(s) 142, 351
Kzo9I GATC 4 cut(s) 37, 142, 268, 351
LpnPI CCDG 9 cut(s) 79, 92, 93, 163, 165, 244, 342, 356, 383
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 214
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 124
MalI GATC 4 cut(s) 39, 144, 270, 353
MboI GATC 4 cut(s) 37, 142, 268, 351
MboII GAAGA 2 cut(s) 71, 218
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 378
MlsI TGGCCA 1 cut(s) 68
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 1 cut(s) 310
MnlI CCTC 2 cut(s) 55, 146
Mox20I TGGCCA 1 cut(s) 68
MroI TCCGGA 1 cut(s) 328
MscI TGGCCA 1 cut(s) 68
MseI TTAA 2 cut(s) 287, 383
MslI CAYNNNNRTG 1 cut(s) 222
Msp20I TGGCCA 1 cut(s) 68
MspI CCGG 2 cut(s) 150, 329
MspR9I CCNGG 2 cut(s) 150, 371
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 1 cut(s) 150
NdeII GATC 4 cut(s) 37, 142, 268, 351
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 1 cut(s) 375
PfoI TCCNGGA 1 cut(s) 148
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 310
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
PpsI GAGTC 1 cut(s) 310
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 369
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 1 cut(s) 375
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 3 cut(s) 119, 251, 323
RsaNI GTAC 3 cut(s) 118, 250, 322
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 2 cut(s) 287, 383
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 4 cut(s) 37, 142, 268, 351
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 2 cut(s) 150, 371
SduI GDGCHC 1 cut(s) 378
SetI ASST 4 cut(s) 48, 54, 66, 117
SfaNI GCATC 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 222
SsiI CCGC 1 cut(s) 311
StyD4I CCNGG 2 cut(s) 148, 369
TaaI ACNGT 2 cut(s) 280, 366
TaiI ACGT 1 cut(s) 54
TatI WGTACW 2 cut(s) 249, 321
Tru1I TTAA 2 cut(s) 287, 383
Tru9I TTAA 2 cut(s) 287, 383
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 124, 174
TspGWI ACGGA 3 cut(s) 261, 307, 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.