pycom01g23460

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0016511
Physical Location & Seq
Forward (+)
20765630 .. 20766013
384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23460.1

Sequence Viewer

Length: 297 bp
ATGGATGATCATCCCGGAGGCGATGAAGTTCTTCTATCTGCAACTGGGAAAGATGCAACAAATGATTTCGAAGATGTTGGTCACAGTGATTCAGCCAGGGAGATGATGGAGAAGTACTACATTGGTGAGGTTGATCAGTCAACTGTCCCATTGAAACGGACTTACATTCCACCACCACAAGGTCGATACAATCCTGACAAGACATCCGAATTTGTTATCAAGATTTTACAGTTTCTGGTTCCGCTTTTAATCTTGGGGTTGGCCTTCGTTGTCCGTCACTATACCAAGAAAGAGTAG

Protein Analysis

99

Amino Acids

11.08

Weight (kDa)

5.09

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 1 - 44 1.4e-14 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53560
fragaria_vesca FvH4_7g32530
malus_domestica MD01G1226000.v1.1 MD07G1296500.v1.1
prunus_persica Prupe.2G316100_v2.0.a1 Prupe.2G316100_v2.0.a1
pyrus_communis pycom01g23460
rosa_chinensis RchiOBHm_Chr1g0382371
rosa_roxburghii Rroxscaffold_7G00167650
rosa_samantha Rh1AG458500 Rh1BG414800 Rh1CG428900 Rh1DG446900
rosa_wichuraiana Rw1G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 242
AcsI RAATTY 1 cut(s) 209
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 95
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 1 cut(s) 261
ApoI RAATTY 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 30
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 137
AsuII TTCGAA 1 cut(s) 69
BccI CCATC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 97
BclI TGATCA 2 cut(s) 7, 133
BcnI CCSGG 1 cut(s) 15
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 2 cut(s) 15, 97
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 15, 97
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 3 cut(s) 10, 10, 203
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 179
BseNI ACTGG 1 cut(s) 49
BshFI GGCC 1 cut(s) 263
BsiSI CCGG 1 cut(s) 15
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 263
Bsp119I TTCGAA 1 cut(s) 69
Bsp143I GATC 2 cut(s) 7, 133
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 263
BspLI GGNNCC 1 cut(s) 240
BspT104I TTCGAA 1 cut(s) 69
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 7, 133
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 86, 145, 231
BstBI TTCGAA 1 cut(s) 69
BstF5I GGATG 3 cut(s) 10, 10, 203
BstKTI GATC 2 cut(s) 10, 136
BstMBI GATC 2 cut(s) 7, 133
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 2 cut(s) 13, 95
BsuRI GGCC 1 cut(s) 263
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 3 cut(s) 10, 10, 203
BtsIMutI CAGTG 1 cut(s) 91
CaiI CAGNNNCTG 1 cut(s) 235
Csp6I GTAC 1 cut(s) 115
CviJI RGCY 2 cut(s) 95, 263
CviKI_1 RGCY 2 cut(s) 95, 263
CviQI GTAC 1 cut(s) 115
DpnI GATC 2 cut(s) 9, 135
DpnII GATC 2 cut(s) 7, 133
EcoRII CCWGG 1 cut(s) 95
FaiI YATR 1 cut(s) 282
FaqI GGGAC 1 cut(s) 131
FbaI TGATCA 2 cut(s) 7, 133
FokI GGATG 2 cut(s) 17, 190
HaeIII GGCC 1 cut(s) 263
HapII CCGG 1 cut(s) 15
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 1 cut(s) 89
HpaII CCGG 1 cut(s) 15
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 141
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 2 cut(s) 194, 220
Hpy8I GTNNAC 1 cut(s) 141
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 3 cut(s) 86, 145, 231
HpyCH4V TGCA 2 cut(s) 41, 56
Ksp22I TGATCA 2 cut(s) 7, 133
Kzo9I GATC 2 cut(s) 7, 133
LpnPI CCDG 6 cut(s) 28, 30, 82, 109, 207, 221
LweI GCATC 1 cut(s) 43
MaeIII GTNAC 2 cut(s) 80, 275
MalI GATC 2 cut(s) 9, 135
MboI GATC 2 cut(s) 7, 133
MboII GAAGA 2 cut(s) 23, 83
MluCI AATT 1 cut(s) 209
MnlI CCTC 2 cut(s) 11, 121
MroXI GAANNNNTTC 1 cut(s) 30
MseI TTAA 1 cut(s) 248
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 2 cut(s) 15, 97
MvaI CCWGG 1 cut(s) 97
NciI CCSGG 1 cut(s) 15
NdeII GATC 2 cut(s) 7, 133
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 2 cut(s) 80, 275
NspV TTCGAA 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 30
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 240
PstNI CAGNNNCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SaqAI TTAA 1 cut(s) 248
Sau3AI GATC 2 cut(s) 7, 133
ScaI AGTACT 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 15, 97
SetI ASST 2 cut(s) 132, 184
SfaNI GCATC 1 cut(s) 43
SfuI TTCGAA 1 cut(s) 69
Sse9I AATT 1 cut(s) 209
SsiI CCGC 1 cut(s) 242
StyD4I CCNGG 2 cut(s) 13, 95
TaaI ACNGT 3 cut(s) 86, 145, 231
TaqI TCGA 2 cut(s) 69, 184
TasI AATT 1 cut(s) 209
TatI WGTACW 1 cut(s) 114
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 2 cut(s) 80, 275
Tsp45I GTSAC 2 cut(s) 80, 275
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 2 cut(s) 172, 263
TspRI CASTG 1 cut(s) 91
XapI RAATTY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmnI GAANNNNTTC 1 cut(s) 30
ZrmI AGTACT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.