Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0016511

Physical Location & Seq

Genomic Coordinates Forward (+)

35680111 .. 35681853

Gene Structure 1743 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1296500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 405 bp

ATGGCTTCTCATCCAAAAGTTCACGTATTCGAAGAGGTGGTAAAGCACAACAAGAACGAAGATTGCTGGATTATTATTGCTGGGAAGGTGTATGATGTGACGCCATTCATGGATGATCATCCCGGAGGCGGTGAAGTTCTGCTAGCTGCAATTGGGAAAGATGCAACAAATGATTTCGAAGATATTGGTCACAGTGATTCAGCCAAGGATATGATGGAGAAGTACTACATTGGTGAGATTGATCCATCAAATATCCCATTGAAACGGATTTCCATCCCGGCACAAGGGGGTCGAAAGAATCCTGCCAAGTCATCCGATTTCGTGATCAAGATTTTACAGTTTCTGGTTCCCCTTTTAATCTTGGGGTTGGCCTTTGCTGTCCGTAACTATACCAAGAAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0009507 GO:0009526 GO:0009527 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009707 GO:0009941 GO:0010319 GO:0012505 GO:0016020 GO:0019867 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042170 GO:0042175 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044434 GO:0044435 GO:0044436 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0071944 GO:0098588 GO:0098805 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5

Protein Analysis

135

Amino Acids

14.88

Weight (kDa)

5.94

Isoelectric Point (pI)

38.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	11 - 80	7.6e-31	Cytochrome b5-like Heme/Steroid binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0016511)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53560
fragaria_vesca	FvH4_7g32530
malus_domestica	MD01G1226000.v1.1 MD07G1296500.v1.1
prunus_persica	Prupe.2G316100_v2.0.a1 Prupe.2G316100_v2.0.a1
pyrus_communis	pycom01g23460
rosa_chinensis	RchiOBHm_Chr1g0382371
rosa_roxburghii	Rroxscaffold_7G00167650
rosa_samantha	Rh1AG458500 Rh1BG414800 Rh1CG428900 Rh1DG446900
rosa_wichuraiana	Rw1G041250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	129
AclWI	GGATC	1 cut(s)	236
AcyI	GRCGYC	1 cut(s)	101
AfaI	GTAC	1 cut(s)	224
AfiI	CCNNNNNNNGG	2 cut(s)	128, 284
AgsI	TTSAA	1 cut(s)	262
AluBI	AGCT	1 cut(s)	146
AluI	AGCT	1 cut(s)	146
AlwI	GGATC	1 cut(s)	236
AlwNI	CAGNNNCTG	1 cut(s)	343
AoxI	GGCC	1 cut(s)	369
ApeKI	GCWGC	1 cut(s)	146
AsuC2I	CCSGG	2 cut(s)	123, 278
AsuHPI	GGTGA	2 cut(s)	143, 245
AsuII	TTCGAA	2 cut(s)	30, 177
AsuNHI	GCTAGC	1 cut(s)	142
BbvI	GCAGC	1 cut(s)	133
BccI	CCATC	3 cut(s)	208, 253, 281
BclI	TGATCA	2 cut(s)	115, 324
BcnI	CCSGG	2 cut(s)	123, 278
BfaI	CTAG	1 cut(s)	143
BisI	GCNGC	1 cut(s)	147
BlsI	GCNGC	1 cut(s)	148
BmcAI	AGTACT	1 cut(s)	224
Bme1390I	CCNGG	2 cut(s)	123, 278
BmiI	GGNNCC	1 cut(s)	348
BmrFI	CCNGG	2 cut(s)	123, 278
BmsI	GCATC	1 cut(s)	151
BmtI	GCTAGC	1 cut(s)	146
Bpu14I	TTCGAA	2 cut(s)	30, 177
BpuMI	CCSGG	2 cut(s)	123, 278
BsaAI	YACGTR	1 cut(s)	25
BsaBI	GATNNNNATC	2 cut(s)	117, 272
BsaHI	GRCGYC	1 cut(s)	101
BsaJI	CCNNGG	1 cut(s)	204
Bsc4I	CCNNNNNNNGG	2 cut(s)	128, 284
Bse8I	GATNNNNATC	2 cut(s)	117, 272
BseDI	CCNNGG	1 cut(s)	204
BseGI	GGATG	5 cut(s)	10, 118, 118, 273, 311
BseJI	GATNNNNATC	2 cut(s)	117, 272
BseLI	CCNNNNNNNGG	2 cut(s)	128, 284
BseXI	GCAGC	1 cut(s)	133
BseYI	CCCAGC	1 cut(s)	80
BshFI	GGCC	1 cut(s)	371
BsiSI	CCGG	2 cut(s)	123, 278
BslI	CCNNNNNNNGG	2 cut(s)	128, 284
BsnI	GGCC	1 cut(s)	371
Bsp119I	TTCGAA	2 cut(s)	30, 177
Bsp143I	GATC	3 cut(s)	115, 241, 324
BspACI	CCGC	1 cut(s)	129
BspANI	GGCC	1 cut(s)	371
BspLI	GGNNCC	1 cut(s)	348
BspOI	GCTAGC	1 cut(s)	146
BspPI	GGATC	1 cut(s)	236
BspT104I	TTCGAA	2 cut(s)	30, 177
BssECI	CCNNGG	1 cut(s)	204
BssMI	GATC	3 cut(s)	115, 241, 324
BssNI	GRCGYC	1 cut(s)	101
BssT1I	CCWWGG	1 cut(s)	204
Bst4CI	ACNGT	2 cut(s)	194, 339
Bst6I	CTCTTC	1 cut(s)	27
BstACI	GRCGYC	1 cut(s)	101
BstBAI	YACGTR	1 cut(s)	25
BstBI	TTCGAA	2 cut(s)	30, 177
BstC8I	GCNNGC	1 cut(s)	144
BstF5I	GGATG	5 cut(s)	10, 118, 118, 273, 311
BstKTI	GATC	3 cut(s)	118, 244, 327
BstMBI	GATC	3 cut(s)	115, 241, 324
BstSCI	CCNGG	2 cut(s)	121, 276
BstV1I	GCAGC	1 cut(s)	133
BsuRI	GGCC	1 cut(s)	371
BtsCI	GGATG	5 cut(s)	10, 118, 118, 273, 311
BtsIMutI	CAGTG	1 cut(s)	199
Cac8I	GCNNGC	1 cut(s)	144
CaiI	CAGNNNCTG	1 cut(s)	343
CseI	GACGC	1 cut(s)	109
Csp6I	GTAC	1 cut(s)	223
CviAII	CATG	1 cut(s)	109
CviJI	RGCY	4 cut(s)	5, 146, 203, 371
CviKI_1	RGCY	4 cut(s)	5, 146, 203, 371
CviQI	GTAC	1 cut(s)	223
DpnI	GATC	3 cut(s)	117, 243, 326
DpnII	GATC	3 cut(s)	115, 241, 324
Eam1104I	CTCTTC	1 cut(s)	27
EarI	CTCTTC	1 cut(s)	27
Eco130I	CCWWGG	1 cut(s)	204
EcoT14I	CCWWGG	1 cut(s)	204
ErhI	CCWWGG	1 cut(s)	204
FaeI	CATG	1 cut(s)	112
FaiI	YATR	4 cut(s)	93, 110, 212, 390
FatI	CATG	1 cut(s)	108
FbaI	TGATCA	2 cut(s)	115, 324
Fnu4HI	GCNGC	1 cut(s)	147
FokI	GGATG	4 cut(s)	105, 125, 260, 298
Fsp4HI	GCNGC	1 cut(s)	147
FspBI	CTAG	1 cut(s)	143
GluI	GCNGC	1 cut(s)	147
GsaI	CCCAGC	1 cut(s)	84
HaeIII	GGCC	1 cut(s)	371
HapII	CCGG	2 cut(s)	123, 278
HgaI	GACGC	1 cut(s)	109
Hin1I	GRCGYC	1 cut(s)	101
Hin1II	CATG	1 cut(s)	112
HinfI	GANTC	2 cut(s)	197, 298
HpaII	CCGG	2 cut(s)	123, 278
HphI	GGTGA	2 cut(s)	143, 245
Hpy166II	GTNNAC	1 cut(s)	22
Hpy188I	TCNGA	1 cut(s)	316
Hpy188III	TCNNGA	2 cut(s)	322, 328
Hpy8I	GTNNAC	1 cut(s)	22
HpyAV	CCTTC	1 cut(s)	79
HpyCH4III	ACNGT	2 cut(s)	194, 339
HpyCH4IV	ACGT	1 cut(s)	24
HpyCH4V	TGCA	2 cut(s)	149, 164
HpySE526I	ACGT	1 cut(s)	24
Hsp92I	GRCGYC	1 cut(s)	101
Hsp92II	CATG	1 cut(s)	112
Ksp22I	TGATCA	2 cut(s)	115, 324
Kzo9I	GATC	3 cut(s)	115, 241, 324
LpnPI	CCDG	6 cut(s)	52, 66, 136, 291, 315, 329
Lsp1109I	GCAGC	1 cut(s)	133
LweI	GCATC	1 cut(s)	151
MaeI	CTAG	1 cut(s)	143
MaeII	ACGT	1 cut(s)	24
MaeIII	GTNAC	3 cut(s)	97, 188, 383
MalI	GATC	3 cut(s)	117, 243, 326
MboI	GATC	3 cut(s)	115, 241, 324
MboII	GAAGA	3 cut(s)	44, 71, 191
MfeI	CAATTG	1 cut(s)	150
MluCI	AATT	1 cut(s)	150
MnlI	CCTC	2 cut(s)	28, 119
MseI	TTAA	1 cut(s)	356
MspI	CCGG	2 cut(s)	123, 278
MspR9I	CCNGG	2 cut(s)	123, 278
MunI	CAATTG	1 cut(s)	150
NciI	CCSGG	2 cut(s)	123, 278
NdeII	GATC	3 cut(s)	115, 241, 324
NheI	GCTAGC	1 cut(s)	142
NlaIII	CATG	1 cut(s)	112
NlaIV	GGNNCC	1 cut(s)	348
NmuCI	GTSAC	2 cut(s)	97, 188
NspV	TTCGAA	2 cut(s)	30, 177
PfeI	GAWTC	2 cut(s)	197, 298
PfoI	TCCNGGA	1 cut(s)	121
PkrI	GCNGC	1 cut(s)	148
Ppu21I	YACGTR	1 cut(s)	25
PspFI	CCCAGC	1 cut(s)	80
PspN4I	GGNNCC	1 cut(s)	348
PstNI	CAGNNNCTG	1 cut(s)	343
RsaI	GTAC	1 cut(s)	224
RsaNI	GTAC	1 cut(s)	223
SaqAI	TTAA	1 cut(s)	356
SatI	GCNGC	1 cut(s)	147
Sau3AI	GATC	3 cut(s)	115, 241, 324
ScaI	AGTACT	1 cut(s)	224
ScrFI	CCNGG	2 cut(s)	123, 278
SetI	ASST	4 cut(s)	27, 39, 90, 148
SfaNI	GCATC	1 cut(s)	151
SfuI	TTCGAA	2 cut(s)	30, 177
Sse9I	AATT	1 cut(s)	150
SsiI	CCGC	1 cut(s)	129
SspMI	CTAG	1 cut(s)	143
StyD4I	CCNGG	2 cut(s)	121, 276
StyI	CCWWGG	1 cut(s)	204
TaaI	ACNGT	2 cut(s)	194, 339
TaiI	ACGT	1 cut(s)	27
TaqI	TCGA	3 cut(s)	30, 177, 292
TasI	AATT	1 cut(s)	150
TatI	WGTACW	1 cut(s)	222
TfiI	GAWTC	2 cut(s)	197, 298
Tru1I	TTAA	1 cut(s)	356
Tru9I	TTAA	1 cut(s)	356
TscAI	CASTG	1 cut(s)	199
TseFI	GTSAC	2 cut(s)	97, 188
TseI	GCWGC	1 cut(s)	146
Tsp45I	GTSAC	2 cut(s)	97, 188
TspDTI	ATGAA	1 cut(s)	97
TspGWI	ACGGA	2 cut(s)	280, 371
TspRI	CASTG	1 cut(s)	199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	211
XspI	CTAG	1 cut(s)	143
ZrmI	AGTACT	1 cut(s)	224

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.