FvH4_7g33561

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009101
Physical Location & Seq
Forward (+)
23843947 .. 23846734
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33561.t2

Sequence Viewer

Length: 636 bp
ATGGAGAGAAAGGAGCTCTTGGTTTGCTGTGTTGTGGGTCTTTTGGGGCTGGTATCAGCTGCTACAGGCTTTGCTGCTGAGGCAACAAGAATCAAGGGTTCTCAGGTTCAGTTTGTCAATGCTACTCAATGCGAATATCCTCGGACTGCAGCTCTTGGACTTGGTTTTACTGCTGCAGTGTCTCTTATGGTAGCTCATATAATTATCAATGTTTCAACAGGGTGCATTTGTTGCAAGAGAATACCCCAACCTTCCAATTCTAACTGGACGATTGCCCTTATCTGCTTTGTTGTGTCCTGGTTCTCATTTGTCATAGCATTTCTTCTGCTGCTCACTGGTTCTGCACTCAATGATCAACATGGTGTAGAAAGCATGTACTTTGGCAGCTACTACTGTTATGTTGTAAAACCTGGAGTATTTGCTGGAGGTGCTGTCCTATCCCTTGCAAGTGTGATCCTAGGAATTTTCTACTACATCACCATAAGTTCAGCAAAGAAGAACAATGATAATCTGTGCGGCAATGGTGTTCAGGGGCCAGCAATAGCTATGGGACAACCCCAGTTTCCAGCACATAATCAAACTCAAGAACCTGTCTTTGTTCATGAAGACACTTATATGAGACGGCAGTTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

212

Amino Acids

22.77

Weight (kDa)

8.07

Isoelectric Point (pI)

27.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 53 - 106 4.9e-11 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 516
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 462
AfaI GTAC 1 cut(s) 377
AgsI TTSAA 1 cut(s) 216
AjnI CCWGG 2 cut(s) 296, 409
AluBI AGCT 6 cut(s) 16, 59, 152, 194, 387, 545
AluI AGCT 6 cut(s) 16, 59, 152, 194, 387, 545
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 2 cut(s) 186, 613
AlwI GGATC 1 cut(s) 448
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 6 cut(s) 59, 74, 149, 173, 328, 384
ApoI RAATTY 1 cut(s) 462
AspA2I CCTAGG 1 cut(s) 457
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 469
AvrII CCTAGG 1 cut(s) 457
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 18
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 6 cut(s) 46, 61, 160, 161, 315, 396
BciT130I CCWGG 2 cut(s) 298, 411
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 2 cut(s) 186, 613
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 3 cut(s) 63, 147, 174
BisI GCNGC 7 cut(s) 60, 75, 150, 174, 329, 385, 517
BlnI CCTAGG 1 cut(s) 457
BlsI GCNGC 7 cut(s) 61, 76, 151, 175, 330, 386, 518
Bme1390I CCNGG 2 cut(s) 298, 411
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 2 cut(s) 298, 411
BmrI ACTGGG 1 cut(s) 553
BmuI ACTGGG 1 cut(s) 553
BpiI GAAGAC 1 cut(s) 612
BpmI CTGGAG 2 cut(s) 432, 444
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 140, 457
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bse1I ACTGG 3 cut(s) 269, 340, 559
Bse3DI GCAATG 1 cut(s) 526
BseBI CCWGG 2 cut(s) 298, 411
BseDI CCNNGG 2 cut(s) 140, 457
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 2 cut(s) 69, 116
BseNI ACTGG 3 cut(s) 269, 340, 559
BseXI GCAGC 6 cut(s) 46, 61, 160, 161, 315, 396
BsgI GTGCAG 1 cut(s) 327
BshFI GGCC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 18
BslFI GGGAC 1 cut(s) 564
BsmAI GTCTC 2 cut(s) 186, 613
BsmBI CGTCTC 1 cut(s) 613
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 352, 453
BspACI CCGC 1 cut(s) 516
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 2 cut(s) 70, 115
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 1 cut(s) 534
BspMAI CTGCAG 2 cut(s) 151, 178
BspPI GGATC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 526
BsrI ACTGG 3 cut(s) 269, 340, 559
BssECI CCNNGG 2 cut(s) 140, 457
BssMI GATC 2 cut(s) 352, 453
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 2 cut(s) 298, 411
Bst4CI ACNGT 1 cut(s) 395
BstAPI GCANNNNNTGC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 2 cut(s) 78, 102
BstKTI GATC 2 cut(s) 355, 456
BstMAI GTCTC 2 cut(s) 186, 613
BstMBI GATC 2 cut(s) 352, 453
BstMWI GCNNNNNNNGC 3 cut(s) 80, 231, 428
BstNI CCWGG 2 cut(s) 298, 411
BstNSI RCATGY 1 cut(s) 376
BstSCI CCNGG 2 cut(s) 296, 409
BstSFI CTRYAG 3 cut(s) 63, 147, 174
BstV1I GCAGC 6 cut(s) 46, 61, 160, 161, 315, 396
BstV2I GAAGAC 1 cut(s) 612
BsuRI GGCC 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 183, 333
Cac8I GCNNGC 1 cut(s) 537
CciI TCATGA 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 376
CviAII CATG 4 cut(s) 359, 373, 602, 633
CviJI RGCY 9 cut(s) 16, 49, 59, 69, 152, 194, 387, 535, 545
CviKI_1 RGCY 9 cut(s) 16, 49, 59, 69, 152, 194, 387, 535, 545
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 78, 102
DpnI GATC 2 cut(s) 354, 455
DpnII GATC 2 cut(s) 352, 453
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 296, 409
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 613
FaeI CATG 4 cut(s) 362, 376, 605, 636
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 564
FatI CATG 4 cut(s) 358, 372, 601, 632
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 7 cut(s) 60, 75, 150, 174, 329, 385, 517
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 7 cut(s) 60, 75, 150, 174, 329, 385, 517
FspBI CTAG 1 cut(s) 458
GluI GCNGC 7 cut(s) 60, 75, 150, 174, 329, 385, 517
GsuI CTGGAG 2 cut(s) 432, 444
HaeIII GGCC 1 cut(s) 535
Hin1II CATG 4 cut(s) 362, 376, 605, 636
HinfI GANTC 1 cut(s) 90
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 1 cut(s) 630
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 2 cut(s) 584, 602
Hpy8I GTNNAC 1 cut(s) 630
HpyAV CCTTC 1 cut(s) 261
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4V TGCA 6 cut(s) 149, 176, 225, 234, 344, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 231, 428
HpyF3I CTNAG 2 cut(s) 78, 102
Hsp92II CATG 4 cut(s) 362, 376, 605, 636
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 2 cut(s) 352, 453
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 6 cut(s) 46, 61, 160, 161, 315, 396
MaeI CTAG 1 cut(s) 458
MalI GATC 2 cut(s) 354, 455
MboI GATC 2 cut(s) 352, 453
MboII GAAGA 3 cut(s) 314, 508, 617
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 3 cut(s) 201, 256, 462
MnlI CCTC 3 cut(s) 73, 150, 419
MslI CAYNNNNRTG 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 298, 411
MvaI CCWGG 2 cut(s) 298, 411
MwoI GCNNNNNNNGC 3 cut(s) 80, 231, 428
NdeII GATC 2 cut(s) 352, 453
NlaIII CATG 4 cut(s) 362, 376, 605, 636
NlaIV GGNNCC 1 cut(s) 534
NspI RCATGY 1 cut(s) 376
PagI TCATGA 1 cut(s) 601
PfeI GAWTC 1 cut(s) 90
PkrI GCNGC 7 cut(s) 61, 76, 151, 175, 330, 386, 518
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 296, 409
PspGI CCWGG 2 cut(s) 296, 409
PspN4I GGNNCC 1 cut(s) 534
PspPI GGNCC 1 cut(s) 533
PstI CTGCAG 2 cut(s) 151, 178
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 1 cut(s) 18
SatI GCNGC 7 cut(s) 60, 75, 150, 174, 329, 385, 517
Sau3AI GATC 2 cut(s) 352, 453
Sau96I GGNCC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 298, 411
SduI GDGCHC 1 cut(s) 18
SfcI CTRYAG 3 cut(s) 63, 147, 174
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 3 cut(s) 201, 256, 462
SsiI CCGC 1 cut(s) 516
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 2 cut(s) 296, 409
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 1 cut(s) 395
TasI AATT 3 cut(s) 201, 256, 462
TatI WGTACW 1 cut(s) 375
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 1 cut(s) 90
TscAI CASTG 2 cut(s) 183, 340
TseI GCWGC 6 cut(s) 59, 74, 149, 173, 328, 384
TspDTI ATGAA 2 cut(s) 590, 618
TspRI CASTG 2 cut(s) 183, 340
XapI RAATTY 1 cut(s) 462
XceI RCATGY 1 cut(s) 376
XmaJI CCTAGG 1 cut(s) 457
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.