MD07G1305500.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009101
Physical Location & Seq
Forward (+)
36246401 .. 36249195
2795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1305500.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGAGAGAAAAGTGCTTCTGGTTTGCTGCGCGGTGGGACTACTGGGGCTGCTATCAGCCGCCACAGGTTTCGGCGCCGAGGGCACGAGAATCAAGGGTTCTCAGGTTCAGTTTGTCTCAGCTGCTCAATGTGAATACCCTCGGAGTCCGGCTCTTGGACTTGGTGTAACTGCTGCAATGGCTCTTATGTTAGCTCAAATAATTCTAAATGTATTTTCTGGGTGCATTTGTTGCAAAAGGAGCCCTCAGCCTTACAACTCTAACTGGACAGTAGCGTTGTTCTGCTTTGTTCTTTCCTGGTTCACATTTGTTATAGCGTTTCTTCTGCTGCTCACTGGTGCCGCACTCAATGACCGACATGGTGTAGAAAGCATGTACTTTGGCAACTACTACTGTTACGTTGTGAAACCCGGAGTGTTTGCCGGAGGTGCCGTTTTGTCAATTGCAAGTGTGGCACTTGGAATTGTCTACTATGTCACCTTAAATTCAGCAAAGAACAGTGATCCTTCATGGGCTGGTTCCACCCCTAATCAAGGGGCAATAGCTATGGGGCAACCTCAGATGCCACAGCAGAGCACTACTACCCAAGAACCAGTATTCGTCCATGAAGACACGTACATGAGGCGGCAGTTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

213

Amino Acids

22.8

Weight (kDa)

8.42

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 151 2.2e-29 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 74, 338, 428
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 32
AciI CCGC 4 cut(s) 32, 60, 342, 625
AclWI GGATC 1 cut(s) 497
AcsI RAATTY 1 cut(s) 484
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 377, 617
AfiI CCNNNNNNNGG 2 cut(s) 155, 533
AflIII ACRYGT 1 cut(s) 612
AjnI CCWGG 1 cut(s) 296
AluBI AGCT 3 cut(s) 122, 194, 545
AluI AGCT 3 cut(s) 122, 194, 545
Alw21I GWGCWC 1 cut(s) 578
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 497
ApeKI GCWGC 5 cut(s) 27, 49, 122, 173, 328
ApoI RAATTY 1 cut(s) 484
AspLEI GCGC 2 cut(s) 32, 77
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 3 cut(s) 74, 338, 428
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 578
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 5 cut(s) 14, 36, 109, 160, 315
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 1 cut(s) 298
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 121
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 8 cut(s) 28, 50, 60, 123, 174, 329, 342, 626
BlsI GCNGC 8 cut(s) 29, 51, 61, 124, 175, 330, 343, 627
Bme1390I CCNGG 2 cut(s) 298, 411
BmiI GGNNCC 5 cut(s) 76, 242, 340, 430, 520
BmrFI CCNGG 2 cut(s) 298, 411
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 552
BmuI ACTGGG 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 615
BplI GAGNNNNNCTC 2 cut(s) 136, 168
Bpu10I CCTNAGC 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 78, 140
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 533
Bse1I ACTGG 4 cut(s) 48, 269, 340, 593
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 78, 140
BseLI CCNNNNNNNGG 2 cut(s) 155, 533
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 4 cut(s) 116, 132, 260, 572
BseNI ACTGG 4 cut(s) 48, 269, 340, 593
BseSI GKGCMC 1 cut(s) 86
BseXI GCAGC 5 cut(s) 14, 36, 109, 160, 315
Bsh1236I CGCG 1 cut(s) 32
BshNI GGYRCC 3 cut(s) 74, 338, 428
BsiHKAI GWGCWC 1 cut(s) 578
BsiSI CCGG 3 cut(s) 149, 411, 423
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 155, 533
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 51
Bsp1286I GDGCHC 3 cut(s) 86, 245, 578
Bsp143I GATC 1 cut(s) 502
BspACI CCGC 4 cut(s) 32, 60, 342, 625
BspCNI CTCAG 4 cut(s) 115, 131, 259, 571
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 5 cut(s) 76, 242, 340, 430, 520
BspPI GGATC 1 cut(s) 497
BspT107I GGYRCC 3 cut(s) 74, 338, 428
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 4 cut(s) 48, 269, 340, 593
BssECI CCNNGG 2 cut(s) 78, 140
BssMI GATC 1 cut(s) 502
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 3 cut(s) 271, 395, 500
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 615
BstDEI CTNAG 4 cut(s) 102, 118, 246, 558
BstENI CCTNNNNNAGG 1 cut(s) 531
BstFNI CGCG 1 cut(s) 32
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 32, 77
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 6 cut(s) 81, 179, 231, 240, 428, 452
BstNI CCWGG 1 cut(s) 298
BstNSI RCATGY 1 cut(s) 376
BstSCI CCNGG 2 cut(s) 296, 409
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 5 cut(s) 14, 36, 109, 160, 315
BstV2I GAAGAC 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 333, 505
CfoI GCGC 2 cut(s) 32, 77
Csp6I GTAC 2 cut(s) 376, 616
CviAII CATG 6 cut(s) 359, 373, 510, 605, 619, 636
CviQI GTAC 2 cut(s) 376, 616
DdeI CTNAG 4 cut(s) 102, 118, 246, 558
DinI GGCGCC 1 cut(s) 76
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
FaeI CATG 6 cut(s) 362, 376, 513, 608, 622, 639
FaqI GGGAC 1 cut(s) 51
FatI CATG 6 cut(s) 358, 372, 509, 604, 618, 635
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 8 cut(s) 28, 50, 60, 123, 174, 329, 342, 626
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 8 cut(s) 28, 50, 60, 123, 174, 329, 342, 626
GlaI GCGC 2 cut(s) 31, 76
GluI GCNGC 8 cut(s) 28, 50, 60, 123, 174, 329, 342, 626
HaeII RGCGCY 1 cut(s) 78
HapII CCGG 3 cut(s) 149, 411, 423
HhaI GCGC 2 cut(s) 32, 77
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 6 cut(s) 362, 376, 513, 608, 622, 639
Hin6I GCGC 2 cut(s) 30, 75
HinP1I GCGC 2 cut(s) 30, 75
HinfI GANTC 2 cut(s) 90, 145
HpaII CCGG 3 cut(s) 149, 411, 423
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 3 cut(s) 303, 469, 633
Hpy188I TCNGA 2 cut(s) 144, 561
Hpy8I GTNNAC 3 cut(s) 303, 469, 633
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 3 cut(s) 271, 395, 500
HpyCH4IV ACGT 2 cut(s) 399, 614
HpyCH4V TGCA 4 cut(s) 176, 225, 234, 446
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 179, 231, 240, 428, 452
HpyF3I CTNAG 4 cut(s) 102, 118, 246, 558
HpySE526I ACGT 2 cut(s) 399, 614
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 6 cut(s) 362, 376, 513, 608, 622, 639
HspAI GCGC 2 cut(s) 30, 75
KasI GGCGCC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 5 cut(s) 14, 36, 109, 160, 315
LweI GCATC 1 cut(s) 552
MaeII ACGT 2 cut(s) 399, 614
MaeIII GTNAC 3 cut(s) 166, 395, 475
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 2 cut(s) 314, 620
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 3 cut(s) 86, 245, 578
MluCI AATT 4 cut(s) 201, 441, 462, 484
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 1 cut(s) 154
MnlI CCTC 6 cut(s) 73, 150, 255, 419, 567, 615
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 3 cut(s) 149, 411, 423
MspR9I CCNGG 2 cut(s) 298, 411
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 1 cut(s) 298
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 6 cut(s) 81, 179, 231, 240, 428, 452
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 1 cut(s) 411
NdeII GATC 1 cut(s) 502
NlaIII CATG 6 cut(s) 362, 376, 513, 608, 622, 639
NlaIV GGNNCC 5 cut(s) 76, 242, 340, 430, 520
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 376
PfeI GAWTC 1 cut(s) 90
PkrI GCNGC 8 cut(s) 29, 51, 61, 124, 175, 330, 343, 627
PleI GAGTC 1 cut(s) 153
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 153
Ppu21I YACGTR 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 5 cut(s) 76, 242, 340, 430, 520
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 377, 617
RsaNI GTAC 2 cut(s) 376, 616
RseI CAYNNNNRTG 1 cut(s) 617
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 8 cut(s) 28, 50, 60, 123, 174, 329, 342, 626
Sau3AI GATC 1 cut(s) 502
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 298, 411
SduI GDGCHC 3 cut(s) 86, 245, 578
SfaNI GCATC 1 cut(s) 552
SfoI GGCGCC 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 617
Sse9I AATT 4 cut(s) 201, 441, 462, 484
SsiI CCGC 4 cut(s) 32, 60, 342, 625
SspDI GGCGCC 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 296, 409
TaaI ACNGT 3 cut(s) 271, 395, 500
TaiI ACGT 2 cut(s) 402, 617
TaqII GACCGA 1 cut(s) 369
TasI AATT 4 cut(s) 201, 441, 462, 484
TatI WGTACW 1 cut(s) 375
TauI GCSGC 3 cut(s) 62, 344, 628
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 2 cut(s) 340, 505
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 5 cut(s) 27, 49, 122, 173, 328
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 498, 621
TspRI CASTG 2 cut(s) 340, 505
XagI CCTNNNNNAGG 1 cut(s) 531
XapI RAATTY 1 cut(s) 484
XceI RCATGY 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.