MD01G1233300.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009101
Physical Location & Seq
Forward (+)
32130351 .. 32132727
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1233300.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGAGAGAAAAGTTCTTCTGGTTTGCTCCGCTGTGGGACTCCTGGGGCTGCTATCTGCGGCTACAGGTTTCGCCGCCGAGGGCACGAGAATCAAGGGTTCTCAGGTTCAATTTGTCTCGAATACTCAATGCGAATACCCTCGGAGTCCGGCTCTTGGACTTGGTGTAACTGCTGCAGTGGCTCTTATGTTAGCTCACATAATTGTAAATGTTTCAACCGGGTGCATTTGTTGCAAAAGGAGCCCCCATCCTACCAACTCTAACTGGACGGTAGCTCTGTTCTGCTTTGTTTTTTCCTGGTTCACATTTGTTATAGCGTTTCTTCTGCTGCTCACTGGTGCCGCACTCAATGACCGACATGGTGTAGAAAGCATGTACTTTGGCAGCTACTACTGTTACGTTGTGAAACCGGGAGTGTTTGCCGGAGGTGCCATTTTGTCATTTGCATGTGTGGCACTCGGAATTGTCTACTATGTAATCTTAAATTCAGCAAAGAACGGTGATACATCATGGGGCGGTTCTGCGCCAACTCAAGGGACAATAGCTATGGGGCAACAACTCCAGATCCCACCGCAGAGTACTACGACCCAAGAACCAGTATTTGTCCATGAAGATACGTATATGAGACGGCAATTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

214

Amino Acids

22.86

Weight (kDa)

8.43

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 151 6.4e-29 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 338, 428
AccI GTMKAC 1 cut(s) 468
AciI CCGC 6 cut(s) 30, 59, 75, 342, 516, 572
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 1 cut(s) 484
AfaI GTAC 2 cut(s) 377, 580
AfiI CCNNNNNNNGG 2 cut(s) 155, 533
AgsI TTSAA 2 cut(s) 110, 216
AjnI CCWGG 2 cut(s) 42, 296
AluBI AGCT 4 cut(s) 194, 275, 387, 545
AluI AGCT 4 cut(s) 194, 275, 387, 545
Alw26I GTCTC 2 cut(s) 121, 619
AlwI GGATC 1 cut(s) 559
ApeKI GCWGC 4 cut(s) 49, 173, 328, 384
ApoI RAATTY 1 cut(s) 484
AspLEI GCGC 1 cut(s) 526
AsuC2I CCSGG 2 cut(s) 220, 411
AsuHPI GGTGA 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 338, 428
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 85
BbvI GCAGC 4 cut(s) 36, 160, 315, 396
BccI CCATC 1 cut(s) 255
BciT130I CCWGG 2 cut(s) 44, 298
BcnI CCSGG 2 cut(s) 220, 411
BcoDI GTCTC 2 cut(s) 121, 619
BfmI CTRYAG 2 cut(s) 63, 174
BisI GCNGC 7 cut(s) 50, 60, 75, 174, 329, 342, 385
BlsI GCNGC 7 cut(s) 51, 61, 76, 175, 330, 343, 386
BmcAI AGTACT 1 cut(s) 580
Bme1390I CCNGG 4 cut(s) 44, 220, 298, 411
BmiI GGNNCC 3 cut(s) 242, 340, 430
BmrFI CCNGG 4 cut(s) 44, 220, 298, 411
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 220, 411
BsaAI YACGTR 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 43, 78, 140
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 533
Bse1I ACTGG 3 cut(s) 269, 340, 596
BseBI CCWGG 2 cut(s) 44, 298
BseDI CCNNGG 3 cut(s) 43, 78, 140
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 155, 533
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 3 cut(s) 269, 340, 596
BseSI GKGCMC 1 cut(s) 86
BseXI GCAGC 4 cut(s) 36, 160, 315, 396
BshNI GGYRCC 2 cut(s) 338, 428
BsiSI CCGG 4 cut(s) 149, 219, 410, 423
BslFI GGGAC 2 cut(s) 51, 550
BslI CCNNNNNNNGG 2 cut(s) 155, 533
BsmAI GTCTC 2 cut(s) 121, 619
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 2 cut(s) 51, 550
Bsp1286I GDGCHC 2 cut(s) 86, 245
Bsp143I GATC 1 cut(s) 564
BspACI CCGC 6 cut(s) 30, 59, 75, 342, 516, 572
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 3 cut(s) 242, 340, 430
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 559
BspT107I GGYRCC 2 cut(s) 338, 428
BsrI ACTGG 3 cut(s) 269, 340, 596
BssECI CCNNGG 3 cut(s) 43, 78, 140
BssMI GATC 1 cut(s) 564
BssSI CACGAG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 44, 298
Bst4CI ACNGT 3 cut(s) 271, 395, 500
BstAPI GCANNNNNTGC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 247
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 1 cut(s) 567
BstMAI GTCTC 2 cut(s) 121, 619
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 5 cut(s) 179, 231, 240, 428, 452
BstNI CCWGG 2 cut(s) 44, 298
BstNSI RCATGY 2 cut(s) 376, 450
BstSCI CCNGG 4 cut(s) 42, 218, 296, 409
BstSFI CTRYAG 2 cut(s) 63, 174
BstSLI GKGCMC 1 cut(s) 86
BstSNI TACGTA 1 cut(s) 618
BstV1I GCAGC 4 cut(s) 36, 160, 315, 396
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 183, 333
CfoI GCGC 1 cut(s) 526
Csp6I GTAC 2 cut(s) 376, 579
CviAII CATG 6 cut(s) 359, 373, 447, 510, 608, 639
CviJI RGCY 9 cut(s) 49, 62, 152, 182, 194, 243, 275, 387, 545
CviKI_1 RGCY 9 cut(s) 49, 62, 152, 182, 194, 243, 275, 387, 545
CviQI GTAC 2 cut(s) 376, 579
DdeI CTNAG 1 cut(s) 102
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
Eco105I TACGTA 1 cut(s) 618
Eco24I GRGCYC 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 42, 296
EcoT38I GRGCYC 1 cut(s) 245
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 6 cut(s) 362, 376, 450, 513, 611, 642
FaqI GGGAC 2 cut(s) 51, 550
FatI CATG 6 cut(s) 358, 372, 446, 509, 607, 638
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 7 cut(s) 50, 60, 75, 174, 329, 342, 385
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 7 cut(s) 50, 60, 75, 174, 329, 342, 385
GlaI GCGC 1 cut(s) 525
GluI GCNGC 7 cut(s) 50, 60, 75, 174, 329, 342, 385
GsuI CTGGAG 1 cut(s) 545
HapII CCGG 4 cut(s) 149, 219, 410, 423
HhaI GCGC 1 cut(s) 526
Hin1II CATG 6 cut(s) 362, 376, 450, 513, 611, 642
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HinfI GANTC 3 cut(s) 39, 90, 145
HpaII CCGG 4 cut(s) 149, 219, 410, 423
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 2 cut(s) 303, 469
Hpy188I TCNGA 2 cut(s) 144, 461
Hpy188III TCNNGA 2 cut(s) 118, 562
Hpy8I GTNNAC 2 cut(s) 303, 469
HpyCH4III ACNGT 3 cut(s) 271, 395, 500
HpyCH4IV ACGT 2 cut(s) 399, 617
HpyCH4V TGCA 4 cut(s) 176, 225, 234, 446
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 231, 240, 428, 452
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 2 cut(s) 399, 617
Hsp92II CATG 6 cut(s) 362, 376, 450, 513, 611, 642
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 1 cut(s) 564
LmnI GCTCC 2 cut(s) 32, 240
Lsp1109I GCAGC 4 cut(s) 36, 160, 315, 396
MaeII ACGT 2 cut(s) 399, 617
MaeIII GTNAC 2 cut(s) 166, 395
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 3 cut(s) 8, 314, 623
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 2 cut(s) 86, 245
MluCI AATT 5 cut(s) 110, 201, 462, 484, 632
MlyI GAGTC 2 cut(s) 33, 154
MnlI CCTC 3 cut(s) 73, 150, 419
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 4 cut(s) 149, 219, 410, 423
MspR9I CCNGG 4 cut(s) 44, 220, 298, 411
MvaI CCWGG 2 cut(s) 44, 298
MwoI GCNNNNNNNGC 5 cut(s) 179, 231, 240, 428, 452
NciI CCSGG 2 cut(s) 220, 411
NdeII GATC 1 cut(s) 564
NlaIII CATG 6 cut(s) 362, 376, 450, 513, 611, 642
NlaIV GGNNCC 3 cut(s) 242, 340, 430
NmeAIII GCCGAG 1 cut(s) 103
NspI RCATGY 2 cut(s) 376, 450
PfeI GAWTC 1 cut(s) 90
PkrI GCNGC 7 cut(s) 51, 61, 76, 175, 330, 343, 386
PleI GAGTC 2 cut(s) 33, 153
PpsI GAGTC 2 cut(s) 33, 153
Ppu21I YACGTR 1 cut(s) 618
Psp6I CCWGG 2 cut(s) 42, 296
PspGI CCWGG 2 cut(s) 42, 296
PspN4I GGNNCC 3 cut(s) 242, 340, 430
PstI CTGCAG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 2 cut(s) 377, 580
RsaNI GTAC 2 cut(s) 376, 579
RseI CAYNNNNRTG 1 cut(s) 445
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 7 cut(s) 50, 60, 75, 174, 329, 342, 385
Sau3AI GATC 1 cut(s) 564
ScaI AGTACT 1 cut(s) 580
SchI GAGTC 2 cut(s) 33, 154
ScrFI CCNGG 4 cut(s) 44, 220, 298, 411
SduI GDGCHC 2 cut(s) 86, 245
SetI ASST 9 cut(s) 70, 108, 196, 277, 389, 402, 430, 547, 620
SfcI CTRYAG 2 cut(s) 63, 174
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
SnaBI TACGTA 1 cut(s) 618
Sse9I AATT 5 cut(s) 110, 201, 462, 484, 632
SsiI CCGC 6 cut(s) 30, 59, 75, 342, 516, 572
StyD4I CCNGG 4 cut(s) 42, 218, 296, 409
TaaI ACNGT 3 cut(s) 271, 395, 500
TaiI ACGT 2 cut(s) 402, 620
TaqI TCGA 1 cut(s) 119
TaqII GACCGA 1 cut(s) 369
TasI AATT 5 cut(s) 110, 201, 462, 484, 632
TatI WGTACW 2 cut(s) 375, 578
TauI GCSGC 3 cut(s) 62, 77, 344
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 2 cut(s) 183, 340
TseI GCWGC 4 cut(s) 49, 173, 328, 384
TspDTI ATGAA 1 cut(s) 624
TspRI CASTG 2 cut(s) 183, 340
XapI RAATTY 1 cut(s) 484
XceI RCATGY 2 cut(s) 376, 450
XmiI GTMKAC 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.