FvH4_7g34110

S-norcoclaurine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007531
Physical Location & Seq
Reverse (-)
24091372 .. 24092289
918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g34110.t1

Sequence Viewer

Length: 513 bp
ATGGTTATAAGCGGGAAAGCTTCGCATGAGTTGGAAGTGGAAGTCCCTGCAAGCCAAGCGTGGGAGCTGTATGGCACTCTTGGGATCGCTAGGCTCGTGGAACAATCTCTCTCCGATGTCATTTCCAAAATTGAAGTCGAACAAGGCGACGGAGAACAAGGCACCATTCTCAAACTAACATTTGCACCAGGGATACCAGGGCCTGGATGGCATAAGGAGAAGTTCACAATGGTAGACAATGAAAAGCGGGTGAAAGAAGTGGAGGTGGTTGAAGGTGGATATCGTGAGTTGGGATTTACTTTTTACTTAATTCGCTTCGAAATCACTGAAGCCGGCAGTAGTACTCCTTCCTCCTGCATTATCAAAACCACGGTTGAATATGAGCTCAAGGAAGAGGCTGCTGCCATGGTCTCATTTGTCTCTGTCGACCTCTTCGCAAATATTGCCCAAATTGCAAAACACCATCTCCTCAAAAAACATACAGAGCAACTAAGTTCACAAGTTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

171

Amino Acids

18.77

Weight (kDa)

5.06

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 7 - 158 2.8e-11 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 203
AccI GTMKAC 2 cut(s) 234, 426
AciI CCGC 2 cut(s) 12, 247
AclWI GGATC 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 2 cut(s) 61, 203
AgsI TTSAA 3 cut(s) 134, 272, 377
AjnI CCWGG 3 cut(s) 187, 196, 202
AluBI AGCT 3 cut(s) 20, 67, 385
AluI AGCT 3 cut(s) 20, 67, 385
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 2 cut(s) 415, 424
AlwI GGATC 1 cut(s) 92
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 398, 401
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 262
AsuII TTCGAA 1 cut(s) 318
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 331, 363
BauI CACGAG 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 385, 388
BccI CCATC 2 cut(s) 201, 471
BciT130I CCWGG 3 cut(s) 189, 198, 204
BciVI GTATCC 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 415, 424
BfaI CTAG 1 cut(s) 90
BfuI GTATCC 1 cut(s) 186
BglI GCCNNNNNGGC 1 cut(s) 208
BisI GCNGC 2 cut(s) 399, 402
BlsI GCNGC 2 cut(s) 400, 403
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 3 cut(s) 189, 198, 204
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 3 cut(s) 189, 198, 204
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 371
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 4 cut(s) 188, 197, 369, 405
BsaXI ACNNNNNCTCC 2 cut(s) 450, 480
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 203
Bse118I RCCGGY 1 cut(s) 332
BseBI CCWGG 3 cut(s) 189, 198, 204
BseDI CCNNGG 4 cut(s) 188, 197, 369, 405
BseGI GGATG 1 cut(s) 212
BseLI CCNNNNNNNGG 2 cut(s) 61, 203
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 2 cut(s) 385, 388
BshFI GGCC 1 cut(s) 202
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 2 cut(s) 61, 203
BsmAI GTCTC 2 cut(s) 415, 424
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 1 cut(s) 202
Bso31I GGTCTC 1 cut(s) 415
Bsp119I TTCGAA 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 1 cut(s) 84
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 2 cut(s) 12, 247
BspANI GGCC 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 1 cut(s) 92
BspT104I TTCGAA 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 4 cut(s) 188, 197, 369, 405
BssMI GATC 1 cut(s) 84
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 189, 198, 204
Bst4CI ACNGT 1 cut(s) 373
Bst6I CTCTTC 2 cut(s) 387, 437
BstAPI GCANNNNNTGC 1 cut(s) 443
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 52, 334
BstDEI CTNAG 1 cut(s) 491
BstDSI CCRYGG 2 cut(s) 369, 405
BstF5I GGATG 1 cut(s) 212
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 2 cut(s) 415, 424
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 4 cut(s) 56, 208, 443, 452
BstNI CCWGG 3 cut(s) 189, 198, 204
BstSCI CCNGG 3 cut(s) 187, 196, 202
BstV1I GCAGC 2 cut(s) 385, 388
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 1 cut(s) 202
BtgI CCRYGG 2 cut(s) 369, 405
BtsCI GGATG 1 cut(s) 212
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 52, 334
CaiI CAGNNNCTG 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 200
Csp6I GTAC 1 cut(s) 342
CviAII CATG 2 cut(s) 26, 406
CviJI RGCY 8 cut(s) 20, 54, 67, 94, 202, 332, 385, 398
CviKI_1 RGCY 8 cut(s) 20, 54, 67, 94, 202, 332, 385, 398
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 1 cut(s) 491
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 387, 437
EarI CTCTTC 2 cut(s) 387, 437
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 187, 196, 202
EcoRV GATATC 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 2 cut(s) 29, 409
FaiI YATR 7 cut(s) 8, 27, 72, 213, 381, 407, 480
FaqI GGGAC 1 cut(s) 29
FatI CATG 2 cut(s) 25, 405
FauI CCCGC 2 cut(s) 5, 240
FblI GTMKAC 2 cut(s) 234, 426
Fnu4HI GCNGC 2 cut(s) 399, 402
FokI GGATG 1 cut(s) 219
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 399, 402
FspBI CTAG 1 cut(s) 90
GluI GCNGC 2 cut(s) 399, 402
HaeIII GGCC 1 cut(s) 202
HapII CCGG 1 cut(s) 333
Hin1II CATG 2 cut(s) 29, 409
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 18
HpaII CCGG 1 cut(s) 333
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 4 cut(s) 225, 235, 427, 497
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 4 cut(s) 225, 235, 427, 497
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 266, 357
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4V TGCA 4 cut(s) 50, 185, 357, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 208, 443, 452
HpyF3I CTNAG 1 cut(s) 491
Hsp92II CATG 2 cut(s) 29, 409
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 9 cut(s) 60, 174, 183, 189, 201, 210, 216, 346, 367
Lsp1109I GCAGC 2 cut(s) 385, 388
MaeI CTAG 1 cut(s) 90
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 2 cut(s) 404, 424
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 3 cut(s) 129, 309, 450
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 5 cut(s) 256, 361, 388, 440, 479
MroNI GCCGGC 1 cut(s) 332
MseI TTAA 1 cut(s) 308
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 3 cut(s) 189, 198, 204
MvaI CCWGG 3 cut(s) 189, 198, 204
MwoI GCNNNNNNNGC 4 cut(s) 56, 208, 443, 452
NaeI GCCGGC 1 cut(s) 334
NcoI CCATGG 1 cut(s) 405
NdeII GATC 1 cut(s) 84
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 2 cut(s) 29, 409
NlaIV GGNNCC 1 cut(s) 163
NspV TTCGAA 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 144
PdiI GCCGGC 1 cut(s) 334
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 2 cut(s) 400, 403
PsiI TTATAA 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 3 cut(s) 187, 196, 202
PspGI CCWGG 3 cut(s) 187, 196, 202
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 200
PstNI CAGNNNCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
SacI GAGCTC 1 cut(s) 387
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 2 cut(s) 399, 402
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 200
ScaI AGTACT 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 189, 198, 204
SduI GDGCHC 1 cut(s) 387
SetI ASST 6 cut(s) 22, 69, 267, 277, 387, 432
SfuI TTCGAA 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 3 cut(s) 129, 309, 450
SsiI CCGC 2 cut(s) 12, 247
SspI AATATT 1 cut(s) 442
SspMI CTAG 1 cut(s) 90
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 3 cut(s) 187, 196, 202
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 373
TaqI TCGA 3 cut(s) 138, 318, 426
TasI AATT 3 cut(s) 129, 309, 450
TatI WGTACW 1 cut(s) 341
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 331
TseI GCWGC 2 cut(s) 398, 401
TspDTI ATGAA 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 331
Van91I CCANNNNNTGG 1 cut(s) 203
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XmiI GTMKAC 2 cut(s) 234, 426
XspI CTAG 1 cut(s) 90
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.