FvH4_7g34120

S-norcoclaurine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007531
Physical Location & Seq
Reverse (-)
24092612 .. 24093841
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g34120.t1

Sequence Viewer

Length: 507 bp
ATGGTTGTTGGGGGGCAAGTTACCCACGAGTTAGAGGTTCAAGTGTCGGCAAGCCAGGCATGGGAGCTGATAAGCACCCTTGAGCTCGCCAAACTCATCCAACAAACTCTCACCGATGTAGTTGAAAAAATTGAAGTCGTACAAGGCGACGGACAGGCCGGCACCATTCTCAGAATAACATGTGCACCGGGAGTGCCGGGCCCTGGATGGCAAACGGAGAAGTTCACAGTGGTGGACAATGAAAAGCGGGTGAAAGTAGCGGAGGTGATTGAAGGTGGATATCTGGAACTAGGGTTTACTCTGTTCCGAGTTCGAGCCGAAATCATAGAGTGCTCCTCCTCCAGCTGCATTATCAAATCCAGCCTTGAGTATGAGCTAAAGGAAGAGGCTGCTGCCAACGTTGCGATCGACTCACTCGTGTCTGCGCATCCATTCGCAAAAATTGCAGAGATCGCCAAAAACCATCTCCTCCTCCAAAACGAAAATAATAGAGACTATGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

169

Amino Acids

18.32

Weight (kDa)

4.69

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 5 - 133 2.8e-10 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 161
AciI CCGC 2 cut(s) 247, 260
AclI AACGTT 1 cut(s) 399
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 61, 203
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 4 cut(s) 41, 125, 134, 272
AjnI CCWGG 2 cut(s) 54, 202
AleI CACNNNNGTG 1 cut(s) 230
AluBI AGCT 4 cut(s) 67, 85, 345, 376
AluI AGCT 4 cut(s) 67, 85, 345, 376
Alw21I GWGCWC 3 cut(s) 87, 187, 335
Alw26I GTCTC 1 cut(s) 486
Alw44I GTGCAC 1 cut(s) 183
AoxI GGCC 2 cut(s) 156, 199
ApaI GGGCCC 1 cut(s) 203
ApaLI GTGCAC 1 cut(s) 183
ApeKI GCWGC 3 cut(s) 345, 389, 392
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 199, 200
AsuC2I CCSGG 2 cut(s) 189, 198
AsuHPI GGTGA 3 cut(s) 103, 262, 277
BaeGI GKGCMC 2 cut(s) 187, 203
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 87, 203
BauI CACGAG 2 cut(s) 26, 416
Bbv12I GWGCWC 3 cut(s) 87, 187, 335
BbvI GCAGC 3 cut(s) 332, 376, 379
BccI CCATC 2 cut(s) 201, 471
BciT130I CCWGG 2 cut(s) 56, 204
BcnI CCSGG 2 cut(s) 189, 198
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 290
BisI GCNGC 3 cut(s) 346, 390, 393
BlsI GCNGC 3 cut(s) 347, 391, 394
Bme1390I CCNGG 4 cut(s) 56, 189, 198, 204
BmgT120I GGNCC 2 cut(s) 199, 200
BmiI GGNNCC 2 cut(s) 163, 201
BmrFI CCNGG 4 cut(s) 56, 189, 198, 204
BmsI GCATC 2 cut(s) 436, 490
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 1 cut(s) 325
BpuEI CTTGAG 2 cut(s) 101, 386
BpuMI CCSGG 2 cut(s) 189, 198
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 203
Bse118I RCCGGY 1 cut(s) 158
BseBI CCWGG 2 cut(s) 56, 204
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 3 cut(s) 96, 212, 427
BseLI CCNNNNNNNGG 2 cut(s) 61, 203
BseMII CTCAG 1 cut(s) 184
BseRI GAGGAG 4 cut(s) 325, 328, 458, 461
BseSI GKGCMC 2 cut(s) 187, 203
BseXI GCAGC 3 cut(s) 332, 376, 379
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 158, 201
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 3 cut(s) 87, 187, 335
BsiSI CCGG 3 cut(s) 159, 188, 197
BslI CCNNNNNNNGG 2 cut(s) 61, 203
BsmAI GTCTC 1 cut(s) 486
BsnI GGCC 2 cut(s) 158, 201
Bsp120I GGGCCC 1 cut(s) 199
Bsp1286I GDGCHC 4 cut(s) 87, 187, 203, 335
Bsp143I GATC 2 cut(s) 405, 450
BspACI CCGC 2 cut(s) 247, 260
BspANI GGCC 2 cut(s) 158, 201
BspCNI CTCAG 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 163, 201
BspT107I GGYRCC 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 2 cut(s) 405, 450
BssSI CACGAG 2 cut(s) 26, 416
Bst2BI CACGAG 2 cut(s) 26, 416
Bst2UI CCWGG 2 cut(s) 56, 204
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 52, 87, 160
BstDEI CTNAG 1 cut(s) 170
BstF5I GGATG 3 cut(s) 96, 212, 427
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 2 cut(s) 408, 453
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 405, 450
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 4 cut(s) 56, 401, 443, 452
BstNI CCWGG 2 cut(s) 56, 204
BstNSI RCATGY 1 cut(s) 183
BstSCI CCNGG 4 cut(s) 54, 187, 196, 202
BstSLI GKGCMC 2 cut(s) 187, 203
BstV1I GCAGC 3 cut(s) 332, 376, 379
BsuRI GGCC 2 cut(s) 158, 201
BtsCI GGATG 3 cut(s) 96, 212, 427
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 3 cut(s) 52, 87, 160
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 199, 200
Csp6I GTAC 1 cut(s) 140
CviAII CATG 3 cut(s) 60, 180, 504
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 1 cut(s) 170
DpnI GATC 2 cut(s) 407, 452
DpnII GATC 2 cut(s) 405, 450
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Ecl136II GAGCTC 1 cut(s) 85
Eco24I GRGCYC 2 cut(s) 87, 203
Eco32I GATATC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 200
EcoRII CCWGG 2 cut(s) 54, 202
EcoRV GATATC 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 505
EcoT38I GRGCYC 2 cut(s) 87, 203
FaeI CATG 3 cut(s) 63, 183, 507
FaiI YATR 6 cut(s) 61, 181, 326, 372, 498, 505
FatI CATG 3 cut(s) 59, 179, 503
FauI CCCGC 1 cut(s) 240
Fnu4HI GCNGC 3 cut(s) 346, 390, 393
FokI GGATG 3 cut(s) 83, 219, 414
FriOI GRGCYC 2 cut(s) 87, 203
Fsp4HI GCNGC 3 cut(s) 346, 390, 393
FspBI CTAG 1 cut(s) 290
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
GluI GCNGC 3 cut(s) 346, 390, 393
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 2 cut(s) 158, 201
HapII CCGG 3 cut(s) 159, 188, 197
HhaI GCGC 1 cut(s) 427
Hin1II CATG 3 cut(s) 63, 183, 507
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 1 cut(s) 410
HpaII CCGG 3 cut(s) 159, 188, 197
HphI GGTGA 3 cut(s) 103, 262, 277
Hpy166II GTNNAC 4 cut(s) 185, 225, 235, 297
Hpy188I TCNGA 2 cut(s) 173, 308
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 4 cut(s) 185, 225, 235, 297
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 4 cut(s) 185, 348, 446, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 401, 443, 452
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 3 cut(s) 63, 183, 507
HspAI GCGC 1 cut(s) 425
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 405, 450
LmnI GCTCC 2 cut(s) 64, 338
Lsp1109I GCAGC 3 cut(s) 332, 376, 379
LweI GCATC 2 cut(s) 436, 490
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 407, 452
MboI GATC 2 cut(s) 405, 450
MboII GAAGA 1 cut(s) 395
MhlI GDGCHC 4 cut(s) 87, 187, 203, 335
MluCI AATT 2 cut(s) 129, 441
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 7 cut(s) 28, 256, 346, 349, 379, 479, 482
Mph1103I ATGCAT 1 cut(s) 505
MroNI GCCGGC 1 cut(s) 158
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 3 cut(s) 159, 188, 197
MspR9I CCNGG 4 cut(s) 56, 189, 198, 204
MvaI CCWGG 2 cut(s) 56, 204
MwoI GCNNNNNNNGC 4 cut(s) 56, 401, 443, 452
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 2 cut(s) 189, 198
NdeII GATC 2 cut(s) 405, 450
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 3 cut(s) 63, 183, 507
NlaIV GGNNCC 2 cut(s) 163, 201
NsbI TGCGCA 1 cut(s) 426
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 1 cut(s) 183
OliI CACNNNNGTG 1 cut(s) 230
PciI ACATGT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 405, 414
PdiI GCCGGC 1 cut(s) 160
PkrI GCNGC 3 cut(s) 347, 391, 394
Ple19I CGATCG 1 cut(s) 408
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PscI ACATGT 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 54, 202
PspGI CCWGG 2 cut(s) 54, 202
PspN4I GGNNCC 2 cut(s) 163, 201
PspOMI GGGCCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 199, 200
PvuI CGATCG 1 cut(s) 408
PvuII CAGCTG 1 cut(s) 345
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 1 cut(s) 230
SacI GAGCTC 1 cut(s) 87
SatI GCNGC 3 cut(s) 346, 390, 393
Sau3AI GATC 2 cut(s) 405, 450
Sau96I GGNCC 2 cut(s) 199, 200
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 4 cut(s) 56, 189, 198, 204
SduI GDGCHC 4 cut(s) 87, 187, 203, 335
SetI ASST 8 cut(s) 39, 69, 87, 267, 277, 347, 378, 402
SfaNI GCATC 2 cut(s) 436, 490
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 2 cut(s) 80, 365
SmoI CTYRAG 2 cut(s) 80, 365
Sse9I AATT 2 cut(s) 129, 441
SsiI CCGC 2 cut(s) 247, 260
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 4 cut(s) 54, 187, 196, 202
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 402
TaqI TCGA 2 cut(s) 313, 408
TasI AATT 2 cut(s) 129, 441
TscAI CASTG 1 cut(s) 234
TseI GCWGC 3 cut(s) 345, 389, 392
TspDTI ATGAA 1 cut(s) 255
TspGWI ACGGA 2 cut(s) 165, 230
TspRI CASTG 1 cut(s) 234
VneI GTGCAC 1 cut(s) 183
XceI RCATGY 1 cut(s) 183
XspI CTAG 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.