pycom09g16230

S-norcoclaurine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007531
Physical Location & Seq
Forward (+)
16409679 .. 16410556
878 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16230.2

Sequence Viewer

Length: 489 bp
ATGGCGATTGGGAAAGTTTGGCATGAATTAGAAGTGGAGGTGCCTGCAAGTGAAGCTTGGGAGCTCTTCGGCACGCTTGCACTGGCAAGATTCGTGGAACAACAACTTCCAGATATGATTGAAAAAGCTGAAGCCATTCATGGTGATGGAGGGGTTGGGACTATTGTCCATGTCAAATTTGCATCAGGTTCTGAACACAAGGAAAAGTTCACAAAGATAGACAATGAAACGCGAGTGAAAGAAGTGGAGGTGATTGAAGGTGGATTTCTGGATCTTGGACTTAGCTTATACCGGGTTCGGTTTGAAATCATAGAGAAATTAGGAGCTGCAGGTGATTCGGAACCTTCCTGCTGCTGCATCATCAAAACCACAGTTGAATATGATGTCAAGGAAGAGACGTCTGATGCTTCCAACGCCTCCTCACTTGTCTCTATCGAGCCATTCGTTACCATTGCAGAGGCAGCAAAGATCCATCTAATGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

163

Amino Acids

17.78

Weight (kDa)

4.84

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
AatII GACGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 40
AccII CGCG 1 cut(s) 232
AclWI GGATC 2 cut(s) 279, 463
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 398
AgsI TTSAA 4 cut(s) 122, 257, 305, 377
AluBI AGCT 5 cut(s) 56, 64, 128, 285, 326
AluI AGCT 5 cut(s) 56, 64, 128, 285, 326
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 2 cut(s) 389, 433
AlwI GGATC 2 cut(s) 279, 463
AlwNI CAGNNNCTG 1 cut(s) 191
ApeKI GCWGC 4 cut(s) 326, 351, 354, 461
ApoI RAATTY 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 135
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 155, 262, 344
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 4 cut(s) 313, 338, 341, 473
BccI CCATC 2 cut(s) 140, 480
BcgI CGANNNNNNTGC 2 cut(s) 318, 352
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 389, 433
BfmI CTRYAG 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 320
BisI GCNGC 4 cut(s) 327, 352, 355, 462
BlsI GCNGC 4 cut(s) 328, 353, 356, 463
Bme1390I CCNGG 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 42, 342
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 3 cut(s) 191, 366, 394
BoxI GACNNNNGTC 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 450
BseMI GCAATG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 409
BseXI GCAGC 4 cut(s) 313, 338, 341, 473
Bsh1236I CGCG 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 172
BsmAI GTCTC 2 cut(s) 389, 433
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 2 cut(s) 271, 468
BspFNI CGCG 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 42, 342
BspMAI CTGCAG 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 2 cut(s) 279, 463
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 1 cut(s) 87
BssMI GATC 2 cut(s) 271, 468
BssNI GRCGYC 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 373
Bst6I CTCTTC 2 cut(s) 71, 387
BstACI GRCGYC 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 45, 74, 78
BstDEI CTNAG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 232
BstKTI GATC 2 cut(s) 274, 471
BstMAI GTCTC 2 cut(s) 389, 433
BstMBI GATC 2 cut(s) 271, 468
BstMWI GCNNNNNNNGC 3 cut(s) 53, 413, 461
BstPAI GACNNNNGTC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 291
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 1 cut(s) 232
BstV1I GCAGC 4 cut(s) 313, 338, 341, 473
BstX2I RGATCY 2 cut(s) 271, 468
BstYI RGATCY 2 cut(s) 271, 468
BtsIMutI CAGTG 1 cut(s) 80
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 3 cut(s) 45, 74, 78
CaiI CAGNNNCTG 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 75, 110
CviAII CATG 3 cut(s) 23, 140, 170
CviJI RGCY 7 cut(s) 56, 64, 128, 134, 285, 326, 439
CviKI_1 RGCY 7 cut(s) 56, 64, 128, 134, 285, 326, 439
DdeI CTNAG 1 cut(s) 281
DpnI GATC 2 cut(s) 273, 470
DpnII GATC 2 cut(s) 271, 468
Eam1104I CTCTTC 2 cut(s) 71, 387
EarI CTCTTC 2 cut(s) 71, 387
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 3 cut(s) 26, 143, 173
FaiI YATR 7 cut(s) 24, 116, 141, 171, 289, 311, 381
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FaqI GGGAC 1 cut(s) 172
FatI CATG 3 cut(s) 22, 139, 169
Fnu4HI GCNGC 4 cut(s) 327, 352, 355, 462
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 4 cut(s) 327, 352, 355, 462
GluI GCNGC 4 cut(s) 327, 352, 355, 462
HapII CCGG 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 3 cut(s) 26, 143, 173
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 2 cut(s) 90, 335
HpaII CCGG 1 cut(s) 292
HphI GGTGA 3 cut(s) 155, 262, 344
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 3 cut(s) 193, 340, 403
Hpy188III TCNNGA 2 cut(s) 110, 269
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 2 cut(s) 251, 354
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 6 cut(s) 47, 80, 182, 329, 357, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 413, 461
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 3 cut(s) 26, 143, 173
Kzo9I GATC 2 cut(s) 271, 468
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 2 cut(s) 61, 323
LpnPI CCDG 8 cut(s) 57, 68, 123, 171, 254, 305, 315, 361
Lsp1109I GCAGC 4 cut(s) 313, 338, 341, 473
LweI GCATC 3 cut(s) 191, 366, 394
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 445
MalI GATC 2 cut(s) 273, 470
MboI GATC 2 cut(s) 271, 468
MboII GAAGA 2 cut(s) 58, 404
MflI RGATCY 2 cut(s) 271, 468
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 3 cut(s) 26, 176, 317
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 6 cut(s) 31, 143, 241, 427, 430, 451
MroXI GAANNNNTTC 1 cut(s) 135
MslI CAYNNNNRTG 1 cut(s) 144
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 3 cut(s) 53, 413, 461
NciI CCSGG 1 cut(s) 293
NdeII GATC 2 cut(s) 271, 468
NlaIII CATG 3 cut(s) 26, 143, 173
NlaIV GGNNCC 2 cut(s) 42, 342
PaqCI CACCTGC 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 90, 335
PkrI GCNGC 4 cut(s) 328, 353, 356, 463
PshAI GACNNNNGTC 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 42, 342
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 2 cut(s) 271, 468
RseI CAYNNNNRTG 1 cut(s) 144
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 71
SatI GCNGC 4 cut(s) 327, 352, 355, 462
Sau3AI GATC 2 cut(s) 271, 468
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 191, 366, 394
SfcI CTRYAG 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 144
Sse9I AATT 3 cut(s) 26, 176, 317
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 401
TaqI TCGA 1 cut(s) 435
TasI AATT 3 cut(s) 26, 176, 317
TfiI GAWTC 2 cut(s) 90, 335
TscAI CASTG 1 cut(s) 87
TseI GCWGC 4 cut(s) 326, 351, 354, 461
TspDTI ATGAA 3 cut(s) 39, 128, 240
TspRI CASTG 1 cut(s) 87
XapI RAATTY 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 135
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.