MD09G1243700.v1.1

S-norcoclaurine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007531
Physical Location & Seq
Forward (+)
31139601 .. 31141900
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1243700.v1.1.491

Sequence Viewer

Length: 504 bp
ATGGCGATTGGGAAAGTTTGGCATGAGTTAGAAGTGGAGGTGCCTGCGAGTGAAGCATGGGAGCTCTTCAGCACGCTTGCACTGGCAAGATTCGTGGAACAACAACTTCCAGATATGATTGAAAAAGCTGAAGCCATACATGGTGATGGAGGGGTTGGGACTATTGTCCATGTCAAATTTGCATCAGGTGCTGAACACAAGGAAAAGTTCACAAAGATTGACAATGAGACGCGAGTGAAAGAAGCGGAGGTGATTGAAGGTGGATTTCTGGATTTTGGACTTAGCTTATACCGGGTTCGGTTTGAAATCATAGAGAAATTAGGAGTTGCAGGTGATTCGGAACCCTCCTGCTGCTGCATCATCAAAACCACAGTTGAATATGATGTCAAGGAAGAGACGTCTGATGCTTCCAACGCCTCCTCACTTGTCTCTATCGAGCCATTCGTTACCATTGCAGAGGCAGCAAAGATCCACCTCATGAAAAAACAATTTGTACTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

168

Amino Acids

18.43

Weight (kDa)

4.84

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 5 - 138 1.7e-08 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
AatII GACGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 40
AccII CGCG 1 cut(s) 232
AciI CCGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 463
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 2 cut(s) 52, 150
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 1 cut(s) 495
AgsI TTSAA 4 cut(s) 122, 257, 305, 377
AluBI AGCT 3 cut(s) 64, 128, 285
AluI AGCT 3 cut(s) 64, 128, 285
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 3 cut(s) 221, 389, 433
AlwI GGATC 1 cut(s) 463
AlwNI CAGNNNCTG 1 cut(s) 191
ApeKI GCWGC 3 cut(s) 351, 354, 461
ApoI RAATTY 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 155, 262, 344
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 3 cut(s) 338, 341, 473
BccI CCATC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 318, 352
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 3 cut(s) 221, 389, 433
BfuAI ACCTGC 1 cut(s) 320
BisI GCNGC 3 cut(s) 352, 355, 462
BlsI GCNGC 3 cut(s) 353, 356, 463
Bme1390I CCNGG 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 42, 342
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 3 cut(s) 191, 366, 394
BoxI GACNNNNGTC 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 450
BseMI GCAATG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 409
BseXI GCAGC 3 cut(s) 338, 341, 473
Bsh1236I CGCG 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 172
BsmAI GTCTC 3 cut(s) 221, 389, 433
BsmBI CGTCTC 2 cut(s) 221, 389
BsmFI GGGAC 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 468
BspACI CCGC 1 cut(s) 245
BspFNI CGCG 1 cut(s) 232
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 42, 342
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 463
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 1 cut(s) 87
BssMI GATC 1 cut(s) 468
BssNI GRCGYC 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 373
Bst6I CTCTTC 2 cut(s) 71, 387
BstACI GRCGYC 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 3 cut(s) 45, 74, 78
BstDEI CTNAG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 232
BstKTI GATC 1 cut(s) 471
BstMAI GTCTC 3 cut(s) 221, 389, 433
BstMBI GATC 1 cut(s) 468
BstMWI GCNNNNNNNGC 4 cut(s) 53, 188, 413, 461
BstPAI GACNNNNGTC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 291
BstUI CGCG 1 cut(s) 232
BstV1I GCAGC 3 cut(s) 338, 341, 473
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BtsIMutI CAGTG 1 cut(s) 80
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 3 cut(s) 45, 74, 78
CaiI CAGNNNCTG 1 cut(s) 191
CciI TCATGA 1 cut(s) 477
CseI GACGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 494
CspCI CAANNNNNGTGG 2 cut(s) 75, 110
CviAII CATG 5 cut(s) 23, 57, 140, 170, 478
CviJI RGCY 5 cut(s) 64, 128, 134, 285, 439
CviKI_1 RGCY 5 cut(s) 64, 128, 134, 285, 439
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 1 cut(s) 281
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 71, 387
EarI CTCTTC 2 cut(s) 71, 387
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 52, 150
EcoICRI GAGCTC 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 221, 389
FaeI CATG 5 cut(s) 26, 60, 143, 173, 481
FaqI GGGAC 1 cut(s) 172
FatI CATG 5 cut(s) 22, 56, 139, 169, 477
Fnu4HI GCNGC 3 cut(s) 352, 355, 462
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 3 cut(s) 352, 355, 462
GluI GCNGC 3 cut(s) 352, 355, 462
HapII CCGG 1 cut(s) 292
HgaI GACGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 5 cut(s) 26, 60, 143, 173, 481
HinfI GANTC 2 cut(s) 90, 335
HpaII CCGG 1 cut(s) 292
HphI GGTGA 3 cut(s) 155, 262, 344
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 340, 403
Hpy188III TCNNGA 3 cut(s) 110, 269, 478
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 5 cut(s) 80, 182, 329, 357, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 188, 413, 461
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 5 cut(s) 26, 60, 143, 173, 481
Kzo9I GATC 1 cut(s) 468
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 8 cut(s) 57, 68, 123, 171, 254, 305, 315, 361
Lsp1109I GCAGC 3 cut(s) 338, 341, 473
LweI GCATC 3 cut(s) 191, 366, 394
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 445
MalI GATC 1 cut(s) 470
MboI GATC 1 cut(s) 468
MboII GAAGA 2 cut(s) 58, 404
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 4 cut(s) 176, 317, 488, 499
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 8 cut(s) 31, 143, 241, 355, 427, 430, 451, 485
MseI TTAA 1 cut(s) 502
MslI CAYNNNNRTG 1 cut(s) 144
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 4 cut(s) 53, 188, 413, 461
NciI CCSGG 1 cut(s) 293
NdeII GATC 1 cut(s) 468
NlaIII CATG 5 cut(s) 26, 60, 143, 173, 481
NlaIV GGNNCC 2 cut(s) 42, 342
PagI TCATGA 1 cut(s) 477
PaqCI CACCTGC 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 2 cut(s) 90, 335
PkrI GCNGC 3 cut(s) 353, 356, 463
PshAI GACNNNNGTC 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 42, 342
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 468
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 144
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 502
SatI GCNGC 3 cut(s) 352, 355, 462
Sau3AI GATC 1 cut(s) 468
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 191, 366, 394
SmiMI CAYNNNNRTG 1 cut(s) 144
Sse9I AATT 4 cut(s) 176, 317, 488, 499
SsiI CCGC 1 cut(s) 245
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 401
TaqI TCGA 1 cut(s) 435
TasI AATT 4 cut(s) 176, 317, 488, 499
TatI WGTACW 1 cut(s) 493
TfiI GAWTC 2 cut(s) 90, 335
Tru1I TTAA 1 cut(s) 502
Tru9I TTAA 1 cut(s) 502
TscAI CASTG 1 cut(s) 87
TseI GCWGC 3 cut(s) 351, 354, 461
TspDTI ATGAA 1 cut(s) 494
TspRI CASTG 1 cut(s) 87
XapI RAATTY 1 cut(s) 176
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.