RchiOBHm_Chr1g0384411

S-norcoclaurine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007531
Physical Location & Seq
Reverse (-)
68275015 .. 68276090
1076 bp
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UTR
Exon/CDS
Intron
PRQ60724

Sequence Viewer

Length: 477 bp
ATGGTTAGCGGGCAAGTTTCGCATGAGTTGGAAGTCGAAGTCTCTGCAAGCCAAGCATGGGAGCTGTATGGCACTCTTGGGATCGCTAGGCTCGTGGAACAAACTTTCACCGATGTAATTGACAAAATTGAAGTCGAACAAGGCGATGGAGAACAAGGCACCATTCTGAAACTAACATTTGCACCAGGTTTTCCAGGGCCTGGATGGCATAAGGAGAAGTTCACAATGGTAGACAATGAAAAACGGATGAAGGAAGTTCATGTGATTGAAGGTGGATATCTGGAGTTGGGATTTACTCTCTACTTAGTTCGCTTCGAAATAAAAGAGGCTGGCACTCCTTCCTCCTGCATTATCAGAACCTCCATCGACTATGAGCTCAAGGAAGAGGCTGCTGCCAGCGCCTCATTTGTCTCTATCGACCTCTTCGCAAATATTGCCGAGGTTGCAAAAAACCATTTCCTCAAAAACAAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

158

Amino Acids

17.59

Weight (kDa)

4.82

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 154 8.9e-11 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 1 cut(s) 200
AccI GTMKAC 1 cut(s) 231
AciI CCGC 1 cut(s) 9
AclWI GGATC 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 58, 200
AgsI TTSAA 2 cut(s) 131, 269
AjnI CCWGG 3 cut(s) 184, 193, 199
AluBI AGCT 2 cut(s) 64, 376
AluI AGCT 2 cut(s) 64, 376
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 2 cut(s) 46, 415
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 2 cut(s) 389, 392
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 100
AsuII TTCGAA 1 cut(s) 315
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 378
BauI CACGAG 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 376, 379
BccI CCATC 3 cut(s) 140, 198, 371
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 3 cut(s) 186, 195, 201
BcoDI GTCTC 2 cut(s) 46, 415
BfaI CTAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 402
BglI GCCNNNNNGGC 1 cut(s) 205
BisI GCNGC 2 cut(s) 390, 393
BlsI GCNGC 2 cut(s) 391, 394
Bme1390I CCNGG 3 cut(s) 186, 195, 201
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 186, 195, 201
BpmI CTGGAG 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 194, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 200
BseBI CCWGG 3 cut(s) 186, 195, 201
BseDI CCNNGG 2 cut(s) 194, 438
BseGI GGATG 2 cut(s) 209, 252
BseLI CCNNNNNNNGG 2 cut(s) 58, 200
BseXI GCAGC 2 cut(s) 376, 379
BshFI GGCC 1 cut(s) 199
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 378
BslI CCNNNNNNNGG 2 cut(s) 58, 200
BsmAI GTCTC 2 cut(s) 46, 415
BsnI GGCC 1 cut(s) 199
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 1 cut(s) 9
BspANI GGCC 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 194, 438
BssMI GATC 1 cut(s) 81
BssSI CACGAG 1 cut(s) 92
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 3 cut(s) 186, 195, 201
Bst6I CTCTTC 2 cut(s) 378, 428
BstAPI GCANNNNNTGC 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 4 cut(s) 11, 49, 331, 397
BstDEI CTNAG 1 cut(s) 304
BstF5I GGATG 2 cut(s) 209, 252
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 46, 415
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 6 cut(s) 19, 53, 205, 398, 434, 443
BstNI CCWGG 3 cut(s) 186, 195, 201
BstSCI CCNGG 3 cut(s) 184, 193, 199
BstV1I GCAGC 2 cut(s) 376, 379
BsuRI GGCC 1 cut(s) 199
BtgZI GCGATG 1 cut(s) 159
BtsCI GGATG 2 cut(s) 209, 252
Cac8I GCNNGC 4 cut(s) 11, 49, 331, 397
CaiI CAGNNNCTG 1 cut(s) 200
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 197
CsiI ACCWGGT 1 cut(s) 184
CviAII CATG 3 cut(s) 23, 57, 260
CviJI RGCY 7 cut(s) 51, 64, 91, 199, 329, 376, 389
CviKI_1 RGCY 7 cut(s) 51, 64, 91, 199, 329, 376, 389
DdeI CTNAG 1 cut(s) 304
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 378, 428
EarI CTCTTC 2 cut(s) 378, 428
Ecl136II GAGCTC 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 378
Eco32I GATATC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 184, 193, 199
EcoRV GATATC 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 378
FaeI CATG 3 cut(s) 26, 60, 263
FaiI YATR 6 cut(s) 24, 58, 69, 210, 261, 372
FatI CATG 3 cut(s) 22, 56, 259
FauI CCCGC 1 cut(s) 2
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 390, 393
FokI GGATG 2 cut(s) 216, 259
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 2 cut(s) 390, 393
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 400
GluI GCNGC 2 cut(s) 390, 393
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 1 cut(s) 199
HhaI GCGC 1 cut(s) 401
Hin1II CATG 3 cut(s) 26, 60, 263
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 222, 232
Hpy188I TCNGA 2 cut(s) 168, 356
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 2 cut(s) 222, 232
HpyAV CCTTC 3 cut(s) 244, 263, 348
HpyCH4V TGCA 4 cut(s) 47, 182, 348, 446
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 53, 205, 398, 434, 443
HpyF3I CTNAG 1 cut(s) 304
Hsp92II CATG 3 cut(s) 26, 60, 263
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 2 cut(s) 376, 379
MabI ACCWGGT 1 cut(s) 184
MaeI CTAG 1 cut(s) 87
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MboII GAAGA 2 cut(s) 395, 415
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 2 cut(s) 117, 126
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 8 cut(s) 319, 352, 370, 379, 412, 431, 433, 470
MspR9I CCNGG 3 cut(s) 186, 195, 201
MvaI CCWGG 3 cut(s) 186, 195, 201
MwoI GCNNNNNNNGC 6 cut(s) 19, 53, 205, 398, 434, 443
NdeII GATC 1 cut(s) 81
NlaIII CATG 3 cut(s) 26, 60, 263
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 463
NspV TTCGAA 1 cut(s) 315
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 141
PflMI CCANNNNNTGG 1 cut(s) 200
PkrI GCNGC 2 cut(s) 391, 394
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 3 cut(s) 184, 193, 199
PspGI CCWGG 3 cut(s) 184, 193, 199
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 200
SacI GAGCTC 1 cut(s) 378
SatI GCNGC 2 cut(s) 390, 393
Sau3AI GATC 1 cut(s) 81
Sau96I GGNCC 1 cut(s) 197
ScrFI CCNGG 3 cut(s) 186, 195, 201
SduI GDGCHC 1 cut(s) 378
SetI ASST 7 cut(s) 66, 190, 274, 362, 378, 423, 444
SexAI ACCWGGT 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 2 cut(s) 117, 126
SsiI CCGC 1 cut(s) 9
SspI AATATT 1 cut(s) 433
SspMI CTAG 1 cut(s) 87
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 3 cut(s) 184, 193, 199
TaqI TCGA 5 cut(s) 36, 135, 315, 366, 417
TasI AATT 2 cut(s) 117, 126
TseI GCWGC 2 cut(s) 389, 392
TspDTI ATGAA 3 cut(s) 248, 252, 263
TspGWI ACGGA 1 cut(s) 259
Van91I CCANNNNNTGG 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 201
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.