Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0016925

Physical Location & Seq

Genomic Coordinates Forward (+)

511921 .. 514238

Gene Structure 2318 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1002700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGCCCTTGAACTGCTTTTGTTGTGCCACCGAAGAAGATCGAGAACCAGCTGAGACCAGGGTTCACAAGAACAGGGATTACCCGTGGGAAGTATATTCTCTCAAGGAGCTTCTTCATGCAACGAACAGCTTCCACCATGATAACAAAATTGGAGAAGGAGGGTTTGGAAGTGTTTATTGGGGTCGAACAAGTAAAGGCATTGCTGTGAAACGATTAAAGACAATGAGTCCAAAGGCAGAGATGGAGTTTGCAGTGGAAGTTGAAATACTTGGAAGGGTGAGACACAGAAATCTGTTAGGCCTGCGGGGATTTTATGCCGGAGGTGATGAAAGGCTCATAGTGTACGATTTTATGCCTAATCATAGCTTGATCACCCATTTACATGGCCAACTTGCAGCAGATTGTCTTCTTGATTGGTCAACAAGACTGAGCATCGCCATTGGATCAGCTGAAGGTTTATCGTACCTGCACCATGAGGCCAATCCTCATATAATTCACAGGGACATAAAGGCCAGTAATGTGCTTCTAGATTCTGAATTCGAAGCAAAAGTTGCTGATTTCGGATTTGCAAAGCTGATTCCGGACGGTGTAACTCATCTGACCACTAGGGTAAAGGGAACTCTTGGATATCTAGCTCCTGAATATGCCATGTGGGGGAAGGTTTCTGAGAGTTGTGATGTTTATAGCTTTGGGATTTTGCTCCTAGAAATAATTAGTGGAAAAAAGCCGATAGAAAAGCTACCAGGAGGAGTGAAACGTGATATTGTTCAATGGGTCACCCCTTATGTCCAAAAGGGTGCATTTAATCAAATTGCTGATCCAAGGTTGAAGGGCAAGTTTGATAAAGTACAAGTGAAATCGACGGTCCTGATTGCTATGAAATGCACCGATAATAGCCCCGACAATCGTCCCCCCATGATAGAGGTGGTGGATTGGCTTAAGGGAGGTATAGGAAGAAGGAAAAAGGAGATCACAAAAGTGGAGGACACTGAGGATGATGAAGGCAGCGAAGGTTACGATGAGAGCCAACCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

345

Amino Acids

38.58

Weight (kDa)

6.24

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	44 - 257	3.4e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	46 - 310	1.6e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016925)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g28620
malus_domestica	MD00G1002700.v1.1 MD01G1191900.v1.1
prunus_persica	Prupe.2G286200_v2.0.a1
pyrus_communis	pycom01g20300 pycom07g23870
rosa_chinensis	RchiOBHm_Chr1g0376081
rosa_multiflora	Rmu_ssc0000116.1_g000028
rosa_roxburghii	Rroxscaffold_4G00282400
rosa_rugosa	Rorug01G0393800
rosa_samantha	Rh1AG405200 Rh1BG368800 Rh1DG398700
rosa_wichuraiana	Rw1G035960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	474
AccIII	TCCGGA	1 cut(s)	580
AciI	CCGC	1 cut(s)	304
AclWI	GGATC	2 cut(s)	451, 812
AcoI	YGGCCR	1 cut(s)	385
AcsI	RAATTY	1 cut(s)	536
AcuI	CTGAAG	1 cut(s)	471
AfaI	GTAC	3 cut(s)	344, 464, 849
AfiI	CCNNNNNNNGG	1 cut(s)	654
AflII	CTTAAG	1 cut(s)	938
AgsI	TTSAA	4 cut(s)	10, 263, 770, 829
AhdI	GACNNNNNGTC	1 cut(s)	225
AjnI	CCWGG	2 cut(s)	56, 742
AjuI	GAANNNNNNNTTGG	4 cut(s)	147, 160, 179, 192
AleI	CACNNNNGTG	1 cut(s)	977
AluBI	AGCT	9 cut(s)	50, 109, 129, 366, 449, 574, 635, 687, 739
AluI	AGCT	9 cut(s)	50, 109, 129, 366, 449, 574, 635, 687, 739
Alw26I	GTCTC	2 cut(s)	47, 274
AlwI	GGATC	2 cut(s)	451, 812
Aor13HI	TCCGGA	1 cut(s)	580
AoxI	GGCC	4 cut(s)	298, 385, 477, 510
ApeKI	GCWGC	2 cut(s)	395, 1005
ApoI	RAATTY	1 cut(s)	536
Asp700I	GAANNNNTTC	1 cut(s)	128
AspS9I	GGNCC	1 cut(s)	865
AsuHPI	GGTGA	4 cut(s)	289, 335, 364, 769
AsuII	TTCGAA	1 cut(s)	540
AvaII	GGWCC	1 cut(s)	865
BalI	TGGCCA	1 cut(s)	387
BarI	GAAGNNNNNNTAC	2 cut(s)	249, 281
BbsI	GAAGAC	1 cut(s)	398
BbvI	GCAGC	2 cut(s)	407, 1017
BccI	CCATC	1 cut(s)	235
BciT130I	CCWGG	2 cut(s)	58, 744
BclI	TGATCA	1 cut(s)	369
BcoDI	GTCTC	2 cut(s)	47, 274
BfaI	CTAG	4 cut(s)	527, 606, 632, 704
BfrI	CTTAAG	1 cut(s)	938
BfuAI	ACCTGC	1 cut(s)	474
BisI	GCNGC	2 cut(s)	396, 1006
BlsI	GCNGC	2 cut(s)	397, 1007
Bme1390I	CCNGG	2 cut(s)	58, 744
Bme18I	GGWCC	1 cut(s)	865
BmeRI	GACNNNNNGTC	1 cut(s)	225
BmgT120I	GGNCC	1 cut(s)	865
BmrFI	CCNGG	2 cut(s)	58, 744
BmsI	GCATC	1 cut(s)	441
BoxI	GACNNNNGTC	1 cut(s)	906
BpiI	GAAGAC	1 cut(s)	398
Bpu14I	TTCGAA	1 cut(s)	540
BpuEI	CTTGAG	1 cut(s)	86
BsaI	GGTCTC	1 cut(s)	47
BsaJI	CCNNGG	3 cut(s)	57, 83, 821
BsaWI	WCCGGW	1 cut(s)	580
Bsc4I	CCNNNNNNNGG	1 cut(s)	654
Bse1I	ACTGG	1 cut(s)	513
Bse3DI	GCAATG	1 cut(s)	198
BseAI	TCCGGA	1 cut(s)	580
BseBI	CCWGG	2 cut(s)	58, 744
BseDI	CCNNGG	3 cut(s)	57, 83, 821
BseGI	GGATG	1 cut(s)	1000
BseLI	CCNNNNNNNGG	1 cut(s)	654
BseMI	GCAATG	1 cut(s)	198
BseMII	CTCAG	4 cut(s)	42, 419, 657, 981
BseNI	ACTGG	1 cut(s)	513
BseRI	GAGGAG	1 cut(s)	762
BseXI	GCAGC	2 cut(s)	407, 1017
BsgI	GTGCAG	1 cut(s)	452
BshFI	GGCC	4 cut(s)	300, 387, 479, 512
BsiSI	CCGG	2 cut(s)	318, 581
BslFI	GGGAC	2 cut(s)	515, 894
BslI	CCNNNNNNNGG	1 cut(s)	654
BsmAI	GTCTC	2 cut(s)	47, 274
BsmFI	GGGAC	2 cut(s)	515, 894
BsnI	GGCC	4 cut(s)	300, 387, 479, 512
Bso31I	GGTCTC	1 cut(s)	47
Bsp119I	TTCGAA	1 cut(s)	540
Bsp13I	TCCGGA	1 cut(s)	580
Bsp143I	GATC	5 cut(s)	37, 369, 443, 817, 969
BspACI	CCGC	1 cut(s)	304
BspANI	GGCC	4 cut(s)	300, 387, 479, 512
BspCNI	CTCAG	4 cut(s)	43, 420, 658, 982
BspEI	TCCGGA	1 cut(s)	580
BspMI	ACCTGC	1 cut(s)	474
BspPI	GGATC	2 cut(s)	451, 812
BspT104I	TTCGAA	1 cut(s)	540
BspTI	CTTAAG	1 cut(s)	938
BspTNI	GGTCTC	1 cut(s)	47
BsrDI	GCAATG	1 cut(s)	198
BsrI	ACTGG	1 cut(s)	513
BssECI	CCNNGG	3 cut(s)	57, 83, 821
BssMI	GATC	5 cut(s)	37, 369, 443, 817, 969
BssT1I	CCWWGG	1 cut(s)	821
Bst2UI	CCWGG	2 cut(s)	58, 744
Bst4CI	ACNGT	2 cut(s)	587, 865
BstAFI	CTTAAG	1 cut(s)	938
BstAPI	GCANNNNNTGC	1 cut(s)	551
BstBI	TTCGAA	1 cut(s)	540
BstC8I	GCNNGC	1 cut(s)	302
BstDEI	CTNAG	4 cut(s)	51, 428, 666, 990
BstDSI	CCRYGG	1 cut(s)	83
BstEII	GGTNACC	1 cut(s)	775
BstF5I	GGATG	1 cut(s)	1000
BstKTI	GATC	5 cut(s)	40, 372, 446, 820, 972
BstMAI	GTCTC	2 cut(s)	47, 274
BstMBI	GATC	5 cut(s)	37, 369, 443, 817, 969
BstMWI	GCNNNNNNNGC	1 cut(s)	551
BstNI	CCWGG	2 cut(s)	58, 744
BstPAI	GACNNNNGTC	1 cut(s)	906
BstPI	GGTNACC	1 cut(s)	775
BstSCI	CCNGG	2 cut(s)	56, 742
BstV1I	GCAGC	2 cut(s)	407, 1017
BstV2I	GAAGAC	1 cut(s)	398
BstXI	CCANNNNNNTGG	1 cut(s)	383
BsuRI	GGCC	4 cut(s)	300, 387, 479, 512
BtgI	CCRYGG	1 cut(s)	83
BtgZI	GCGATG	1 cut(s)	418
BtsCI	GGATG	1 cut(s)	1000
BtsI	GCAGTG	1 cut(s)	258
BtsIMutI	CAGTG	2 cut(s)	258, 987
BveI	ACCTGC	1 cut(s)	474
Cac8I	GCNNGC	1 cut(s)	302
Cfr13I	GGNCC	1 cut(s)	865
Csp6I	GTAC	3 cut(s)	343, 463, 848
CviAII	CATG	6 cut(s)	116, 137, 383, 473, 649, 916
CviQI	GTAC	3 cut(s)	343, 463, 848
DdeI	CTNAG	4 cut(s)	51, 428, 666, 990
DpnI	GATC	5 cut(s)	39, 371, 445, 819, 971
DpnII	GATC	5 cut(s)	37, 369, 443, 817, 969
DriI	GACNNNNNGTC	1 cut(s)	225
EaeI	YGGCCR	1 cut(s)	385
Eam1105I	GACNNNNNGTC	1 cut(s)	225
Eco130I	CCWWGG	1 cut(s)	821
Eco147I	AGGCCT	1 cut(s)	300
Eco31I	GGTCTC	1 cut(s)	47
Eco32I	GATATC	1 cut(s)	629
Eco47I	GGWCC	1 cut(s)	865
Eco57I	CTGAAG	1 cut(s)	471
Eco91I	GGTNACC	1 cut(s)	775
EcoO65I	GGTNACC	1 cut(s)	775
EcoRI	GAATTC	1 cut(s)	536
EcoRII	CCWGG	2 cut(s)	56, 742
EcoRV	GATATC	1 cut(s)	629
EcoT14I	CCWWGG	1 cut(s)	821
ErhI	CCWWGG	1 cut(s)	821
FaeI	CATG	6 cut(s)	119, 140, 386, 476, 652, 919
FaqI	GGGAC	2 cut(s)	515, 894
FatI	CATG	6 cut(s)	115, 136, 382, 472, 648, 915
FauI	CCCGC	1 cut(s)	297
FbaI	TGATCA	1 cut(s)	369
Fnu4HI	GCNGC	2 cut(s)	396, 1006
FokI	GGATG	1 cut(s)	1007
Fsp4HI	GCNGC	2 cut(s)	396, 1006
FspBI	CTAG	4 cut(s)	527, 606, 632, 704
GluI	GCNGC	2 cut(s)	396, 1006
HaeIII	GGCC	4 cut(s)	300, 387, 479, 512
HapII	CCGG	2 cut(s)	318, 581
Hin1II	CATG	6 cut(s)	119, 140, 386, 476, 652, 919
HincII	GTYRAC	1 cut(s)	420
HindII	GTYRAC	1 cut(s)	420
HinfI	GANTC	3 cut(s)	226, 530, 577
HpaII	CCGG	2 cut(s)	318, 581
HphI	GGTGA	4 cut(s)	289, 335, 364, 769
Hpy166II	GTNNAC	3 cut(s)	64, 343, 420
Hpy188I	TCNGA	4 cut(s)	535, 563, 600, 667
Hpy188III	TCNNGA	6 cut(s)	41, 410, 527, 581, 638, 868
Hpy8I	GTNNAC	3 cut(s)	64, 343, 420
Hpy99I	CGWCG	1 cut(s)	865
HpyAV	CCTTC	8 cut(s)	149, 267, 446, 652, 823, 951, 995, 1004
HpyCH4III	ACNGT	2 cut(s)	587, 865
HpyCH4IV	ACGT	1 cut(s)	757
HpyCH4V	TGCA	7 cut(s)	119, 251, 395, 469, 569, 800, 885
HpyF10VI	GCNNNNNNNGC	1 cut(s)	551
HpyF3I	CTNAG	4 cut(s)	51, 428, 666, 990
HpySE526I	ACGT	1 cut(s)	757
Hsp92II	CATG	6 cut(s)	119, 140, 386, 476, 652, 919
Kpn2I	TCCGGA	1 cut(s)	580
Ksp22I	TGATCA	1 cut(s)	369
Kzo9I	GATC	5 cut(s)	37, 369, 443, 817, 969
LmnI	GCTCC	3 cut(s)	106, 640, 705
Lsp1109I	GCAGC	2 cut(s)	407, 1017
LweI	GCATC	1 cut(s)	441
MaeI	CTAG	4 cut(s)	527, 606, 632, 704
MaeII	ACGT	1 cut(s)	757
MaeIII	GTNAC	3 cut(s)	589, 775, 1013
MalI	GATC	5 cut(s)	39, 371, 445, 819, 971
MboI	GATC	5 cut(s)	37, 369, 443, 817, 969
MboII	GAAGA	5 cut(s)	44, 47, 104, 398, 966
MlsI	TGGCCA	1 cut(s)	387
MluCI	AATT	5 cut(s)	147, 492, 536, 711, 810
MluNI	TGGCCA	1 cut(s)	387
MlyI	GAGTC	1 cut(s)	235
MnlI	CCTC	9 cut(s)	152, 314, 469, 495, 740, 916, 938, 976, 985
Mox20I	TGGCCA	1 cut(s)	387
MroI	TCCGGA	1 cut(s)	580
MroXI	GAANNNNTTC	1 cut(s)	128
MscI	TGGCCA	1 cut(s)	387
MseI	TTAA	3 cut(s)	215, 804, 939
MslI	CAYNNNNRTG	3 cut(s)	203, 381, 977
Msp20I	TGGCCA	1 cut(s)	387
MspA1I	CMGCKG	2 cut(s)	50, 449
MspCI	CTTAAG	1 cut(s)	938
MspI	CCGG	2 cut(s)	318, 581
MspR9I	CCNGG	2 cut(s)	58, 744
MvaI	CCWGG	2 cut(s)	58, 744
MwoI	GCNNNNNNNGC	1 cut(s)	551
NdeII	GATC	5 cut(s)	37, 369, 443, 817, 969
NlaIII	CATG	6 cut(s)	119, 140, 386, 476, 652, 919
NmuCI	GTSAC	1 cut(s)	775
NspV	TTCGAA	1 cut(s)	540
OliI	CACNNNNGTG	1 cut(s)	977
PceI	AGGCCT	1 cut(s)	300
PdmI	GAANNNNTTC	1 cut(s)	128
PfeI	GAWTC	2 cut(s)	530, 577
PkrI	GCNGC	2 cut(s)	397, 1007
PleI	GAGTC	1 cut(s)	234
PpsI	GAGTC	1 cut(s)	234
PshAI	GACNNNNGTC	1 cut(s)	906
Psp6I	CCWGG	2 cut(s)	56, 742
PspEI	GGTNACC	1 cut(s)	775
PspGI	CCWGG	2 cut(s)	56, 742
PspPI	GGNCC	1 cut(s)	865
PsrI	GAACNNNNNNTAC	2 cut(s)	62, 94
PvuII	CAGCTG	2 cut(s)	50, 449
RsaI	GTAC	3 cut(s)	344, 464, 849
RsaNI	GTAC	3 cut(s)	343, 463, 848
RseI	CAYNNNNRTG	3 cut(s)	203, 381, 977
SaqAI	TTAA	3 cut(s)	215, 804, 939
SatI	GCNGC	2 cut(s)	396, 1006
Sau3AI	GATC	5 cut(s)	37, 369, 443, 817, 969
Sau96I	GGNCC	1 cut(s)	865
SchI	GAGTC	1 cut(s)	235
ScrFI	CCNGG	2 cut(s)	58, 744
SfaNI	GCATC	1 cut(s)	441
SfuI	TTCGAA	1 cut(s)	540
SinI	GGWCC	1 cut(s)	865
SmiMI	CAYNNNNRTG	3 cut(s)	203, 381, 977
SmlI	CTYRAG	2 cut(s)	101, 938
SmoI	CTYRAG	2 cut(s)	101, 938
Sse9I	AATT	5 cut(s)	147, 492, 536, 711, 810
SseBI	AGGCCT	1 cut(s)	300
SsiI	CCGC	1 cut(s)	304
SspMI	CTAG	4 cut(s)	527, 606, 632, 704
StuI	AGGCCT	1 cut(s)	300
StyD4I	CCNGG	2 cut(s)	56, 742
StyI	CCWWGG	1 cut(s)	821
TaaI	ACNGT	2 cut(s)	587, 865
TaiI	ACGT	1 cut(s)	760
TaqI	TCGA	4 cut(s)	40, 184, 540, 860
TasI	AATT	5 cut(s)	147, 492, 536, 711, 810
TatI	WGTACW	1 cut(s)	847
TfiI	GAWTC	2 cut(s)	530, 577
Tru1I	TTAA	3 cut(s)	215, 804, 939
Tru9I	TTAA	3 cut(s)	215, 804, 939
TscAI	CASTG	2 cut(s)	258, 994
TseFI	GTSAC	1 cut(s)	775
TseI	GCWGC	2 cut(s)	395, 1005
Tsp45I	GTSAC	1 cut(s)	775
TspDTI	ATGAA	4 cut(s)	104, 342, 893, 1014
TspRI	CASTG	2 cut(s)	258, 994
Vha464I	CTTAAG	1 cut(s)	938
VpaK11BI	GGWCC	1 cut(s)	865
XapI	RAATTY	1 cut(s)	536
XbaI	TCTAGA	1 cut(s)	526
XcmI	CCANNNNNNNNNTGG	1 cut(s)	922
XmnI	GAANNNNTTC	1 cut(s)	128
XspI	CTAG	4 cut(s)	527, 606, 632, 704

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.