MD00G1008400.v1.1

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015807
Physical Location & Seq
Forward (+)
1088696 .. 1091673
2978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1008400.v1.1.491

Sequence Viewer

Length: 648 bp
ATGAATGTCTTCAGATTGGCCGGCGACATGACTCACTTGGCCAGTATTCTGATCCTCCTCCTCAAAATTTACGCCACCAAATCGTGCTCAGGGATTTCGCTTAAGACTCAGGAGCTCTATGCCCTGGTGTTTCTGGCTCGGTATTTGGACCTGTTTACGAACTTTATTTCGGTATATAACACTGTGATGAAGCTGGTGTTTATCGGAAGCTCGCTGGCCATTGTTTGGTGTATGCGGATGCACAAAATGGTGAAGCGTTCATACAATAAAGAGCTCGATACGTTCCGGCATTATTTTCTTATTGCTGGCTGCCTTATGCTCGCCCTGCTTGTCAACGAGAAGTTCACGTTTCAAGAGGTGCTCTGGGCATTTTCTATATACTTGGAGGCGGTTTCTATGATTCCGCAGTTAATTTTGCTACAACGAAGTGGAAATGTGGACAATTTGACAGGGCAATATGTGTTCCTTCTTGGGGCCTACCGTGCATTGTACATTCTCAACTGGATCTACCGCTACTTCACAGAACCACACTTCAGTCGATGGATAGCGTGTGTCGCTGGTCTGGTTCAGACAGCTTTATATGCAGATTTTTTCTACTACTATTTTATCAGCTGGAAAAACAATGCAAAGCTTCAGCTGCCAGCGTAA

Protein Analysis

216

Amino Acids

25.1

Weight (kDa)

9.3

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 28 - 171 2.6e-58 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29330 AT1G29330
fragaria_vesca FvH4_3g07240 FvH4_3g07240
malus_domestica MD00G1008400.v1.1
prunus_persica Prupe.4G067600_v2.0.a1
pyrus_communis pycom05g27360 pycom10g22930
rosa_chinensis RchiOBHm_Chr5g0011901
rosa_laevigata RLG00000031866
rosa_multiflora Rmu_sc0001475.1_g000009
rosa_roxburghii Rroxscaffold_1G00064730
rosa_rugosa Rorug04G0459600
rosa_samantha Rh5CG100900
rosa_wichuraiana Rw5G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 225
AciI CCGC 4 cut(s) 235, 389, 404, 511
AclWI GGATC 2 cut(s) 46, 512
AcoI YGGCCR 3 cut(s) 18, 39, 216
AcsI RAATTY 1 cut(s) 66
AcuI CTGAAG 2 cut(s) 517, 617
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 2 cut(s) 225, 472
AflII CTTAAG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 353
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 8 cut(s) 115, 193, 210, 274, 575, 612, 631, 637
AluI AGCT 8 cut(s) 115, 193, 210, 274, 575, 612, 631, 637
Alw21I GWGCWC 4 cut(s) 89, 117, 276, 363
AlwI GGATC 2 cut(s) 46, 512
AoxI GGCC 4 cut(s) 18, 39, 216, 474
ApeKI GCWGC 2 cut(s) 309, 637
ApoI RAATTY 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 148, 474
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 1 cut(s) 148
BalI TGGCCA 2 cut(s) 41, 218
BanII GRGCYC 2 cut(s) 117, 276
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 4 cut(s) 89, 117, 276, 363
BbvI GCAGC 2 cut(s) 296, 624
BccI CCATC 1 cut(s) 534
BciT130I CCWGG 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 101
BisI GCNGC 2 cut(s) 310, 638
BlsI GCNGC 2 cut(s) 311, 639
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 148, 474
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 472
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 2 cut(s) 42, 506
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 2 cut(s) 225, 472
BseMII CTCAG 2 cut(s) 102, 122
BseNI ACTGG 2 cut(s) 42, 506
BseRI GAGGAG 2 cut(s) 47, 50
BseXI GCAGC 2 cut(s) 296, 624
BshFI GGCC 4 cut(s) 20, 41, 218, 476
BsiHKAI GWGCWC 4 cut(s) 89, 117, 276, 363
BsiSI CCGG 2 cut(s) 21, 286
BslI CCNNNNNNNGG 2 cut(s) 225, 472
BsnI GGCC 4 cut(s) 20, 41, 218, 476
Bsp1286I GDGCHC 4 cut(s) 89, 117, 276, 363
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 2 cut(s) 51, 504
BspACI CCGC 4 cut(s) 235, 389, 404, 511
BspANI GGCC 4 cut(s) 20, 41, 218, 476
BspCNI CTCAG 2 cut(s) 101, 121
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 2 cut(s) 46, 512
BspTI CTTAAG 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 2 cut(s) 42, 506
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 2 cut(s) 51, 504
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 184, 482
BstAFI CTTAAG 1 cut(s) 101
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 6 cut(s) 22, 212, 216, 307, 321, 642
BstDEI CTNAG 2 cut(s) 88, 108
BstF5I GGATG 1 cut(s) 243
BstKTI GATC 2 cut(s) 54, 507
BstMBI GATC 2 cut(s) 51, 504
BstMWI GCNNNNNNNGC 5 cut(s) 325, 482, 554, 581, 637
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 296, 624
BstX2I RGATCY 1 cut(s) 504
BstYI RGATCY 1 cut(s) 504
BsuRI GGCC 4 cut(s) 20, 41, 218, 476
BtsCI GGATG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 6 cut(s) 22, 212, 216, 307, 321, 642
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 148, 474
Csp6I GTAC 1 cut(s) 490
CviAII CATG 1 cut(s) 28
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 2 cut(s) 88, 108
DpnI GATC 2 cut(s) 53, 506
DpnII GATC 2 cut(s) 51, 504
EaeI YGGCCR 3 cut(s) 18, 39, 216
Ecl136II GAGCTC 2 cut(s) 115, 274
Eco24I GRGCYC 2 cut(s) 117, 276
Eco47I GGWCC 1 cut(s) 148
Eco53kI GAGCTC 2 cut(s) 115, 274
Eco57I CTGAAG 2 cut(s) 517, 617
EcoICRI GAGCTC 2 cut(s) 115, 274
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 117, 276
FaeI CATG 1 cut(s) 31
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 2 cut(s) 310, 638
FokI GGATG 1 cut(s) 250
FriOI GRGCYC 2 cut(s) 117, 276
Fsp4HI GCNGC 2 cut(s) 310, 638
GluI GCNGC 2 cut(s) 310, 638
HaeIII GGCC 4 cut(s) 20, 41, 218, 476
HapII CCGG 2 cut(s) 21, 286
Hin1II CATG 1 cut(s) 31
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 3 cut(s) 31, 106, 400
HpaII CCGG 2 cut(s) 21, 286
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 4 cut(s) 156, 334, 345, 439
Hpy188I TCNGA 4 cut(s) 14, 51, 206, 570
Hpy188III TCNNGA 2 cut(s) 110, 353
Hpy8I GTNNAC 4 cut(s) 156, 334, 345, 439
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 2 cut(s) 184, 482
HpyCH4IV ACGT 2 cut(s) 281, 347
HpyCH4V TGCA 4 cut(s) 241, 485, 584, 626
HpyF10VI GCNNNNNNNGC 5 cut(s) 325, 482, 554, 581, 637
HpyF3I CTNAG 2 cut(s) 88, 108
HpySE526I ACGT 2 cut(s) 281, 347
Hsp92II CATG 1 cut(s) 31
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 2 cut(s) 51, 504
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 2 cut(s) 296, 624
LweI GCATC 1 cut(s) 228
MaeII ACGT 2 cut(s) 281, 347
MalI GATC 2 cut(s) 53, 506
MboI GATC 2 cut(s) 51, 504
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 4 cut(s) 89, 117, 276, 363
MlsI TGGCCA 2 cut(s) 41, 218
MluCI AATT 3 cut(s) 66, 411, 442
MluNI TGGCCA 2 cut(s) 41, 218
MlyI GAGTC 2 cut(s) 25, 100
MnlI CCTC 5 cut(s) 65, 68, 71, 349, 379
Mox20I TGGCCA 2 cut(s) 41, 218
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 2 cut(s) 41, 218
MseI TTAA 2 cut(s) 102, 410
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 2 cut(s) 41, 218
MspA1I CMGCKG 2 cut(s) 612, 637
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 2 cut(s) 21, 286
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 5 cut(s) 325, 482, 554, 581, 637
NaeI GCCGGC 1 cut(s) 22
NdeII GATC 2 cut(s) 51, 504
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 1 cut(s) 475
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 400
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 2 cut(s) 311, 639
PleI GAGTC 2 cut(s) 25, 100
PpsI GAGTC 2 cut(s) 25, 100
Psp124BI GAGCTC 2 cut(s) 117, 276
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 2 cut(s) 148, 474
PsuI RGATCY 1 cut(s) 504
PvuII CAGCTG 2 cut(s) 612, 637
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 2 cut(s) 117, 276
SaqAI TTAA 2 cut(s) 102, 410
SatI GCNGC 2 cut(s) 310, 638
Sau3AI GATC 2 cut(s) 51, 504
Sau96I GGNCC 2 cut(s) 148, 474
SchI GAGTC 2 cut(s) 25, 100
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 4 cut(s) 89, 117, 276, 363
SfaNI GCATC 1 cut(s) 228
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 3 cut(s) 66, 411, 442
SsiI CCGC 4 cut(s) 235, 389, 404, 511
SstI GAGCTC 2 cut(s) 117, 276
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 184, 482
TaiI ACGT 2 cut(s) 284, 350
TaqI TCGA 2 cut(s) 276, 538
TasI AATT 3 cut(s) 66, 411, 442
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 2 cut(s) 102, 410
Tru9I TTAA 2 cut(s) 102, 410
TscAI CASTG 1 cut(s) 187
TseI GCWGC 2 cut(s) 309, 637
TspDTI ATGAA 3 cut(s) 17, 203, 249
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 225
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.