RchiOBHm_Chr5g0011901

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015807
Physical Location & Seq
Reverse (-)
7919016 .. 7922274
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29251

Sequence Viewer

Length: 876 bp
ATGAAATCCAACCCTCCAACAAATCCAACGCGCCAAAATGCGCCAAGAAACGGGGACCAATCCCGTGCCTTCTGTAAACACCGTGGAGGCGGTAGAAGCCCGTTTCCGACTGGCACGTCACCCTCTCTTCTCTTACACACTCTTTCCACACCTAGTCAATTTTCATTTTCCTCTTCCTCTTCTCTCTGCTTCAATCTAGGGCACCCAAAATCTCCGATCTTTCCGACCATGAACGCCTTCAGGCTCGCCGGCGATATCACCCACCTCATCAGCATCCTCGTCCTCCTCCTCAAAATCTACGCCACCAAATCCTGCTCAGGGATCTCGCTCAAGACTCAGGAGCTCTACGCGGTGGTGTTTCTGGCGCGGTACTTGGATCTGTTCACGGATTTCATTTCGGTCTACAACTCCGTGATGAAGGTGGTGTTCATTCTGAGCTCGGTGGCGATTGTGTGGTGTATGCGGCGGCACAGAGCGGTGAAGCGGTCCTACGATAAGGAGCTGGATACGTTCCGCCATTACTATCTCGTTGGAGCTTGCTTTGTGTTTGCTCTGCTTGTTCATGAGAAGTTCACATTTCAGGAGGTCTTCTGGGCATTTTCAATATACTTGGAGGCAGTTGCAATTATTCCGCAATTAGTTTTGCTACAACGAAGTGGAAATGTGGACAATTTGACAGGGCAGTATGTTTTCTTTCTGGGGGCCTATCGTGCATTGTACATACTCAACTGGATCTACCGCTACTTCACAGAAGCAAAATTCAGTCGATGGATAGCTTGCATCTCTGGTCTGGTTCAGACAGCTTTGTATGCAGATTTTTTCTACTACTACTTTCTCAGTTGGAAAAACAATGCAAAGCTTAACCTGCCAGCTTAA

Protein Analysis

291

Amino Acids

33.28

Weight (kDa)

9.64

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 104 - 247 2.3e-59 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29330 AT1G29330
fragaria_vesca FvH4_3g07240 FvH4_3g07240
malus_domestica MD00G1008400.v1.1
prunus_persica Prupe.4G067600_v2.0.a1
pyrus_communis pycom05g27360 pycom10g22930
rosa_chinensis RchiOBHm_Chr5g0011901
rosa_laevigata RLG00000031866
rosa_multiflora Rmu_sc0001475.1_g000009
rosa_roxburghii Rroxscaffold_1G00064730
rosa_rugosa Rorug04G0459600
rosa_samantha Rh5CG100900
rosa_wichuraiana Rw5G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 201
AccBSI CCGCTC 1 cut(s) 476
AccI GTMKAC 1 cut(s) 402
AccII CGCG 3 cut(s) 31, 350, 367
AclWI GGATC 3 cut(s) 329, 384, 740
AcsI RAATTY 1 cut(s) 758
AcuI CTGAAG 1 cut(s) 223
AfaI GTAC 2 cut(s) 371, 719
AfiI CCNNNNNNNGG 2 cut(s) 50, 318
AgsI TTSAA 2 cut(s) 193, 603
AjiI CACGTC 1 cut(s) 117
AluBI AGCT 8 cut(s) 343, 438, 502, 536, 776, 803, 859, 872
AluI AGCT 8 cut(s) 343, 438, 502, 536, 776, 803, 859, 872
Alw21I GWGCWC 2 cut(s) 345, 440
AlwI GGATC 3 cut(s) 329, 384, 740
AoxI GGCC 1 cut(s) 702
ApoI RAATTY 1 cut(s) 758
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 3 cut(s) 33, 43, 367
AspS9I GGNCC 3 cut(s) 55, 486, 702
AsuHPI GGTGA 3 cut(s) 111, 250, 490
AvaII GGWCC 2 cut(s) 55, 486
BaeGI GKGCMC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 2 cut(s) 345, 440
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 2 cut(s) 345, 440
BccI CCATC 1 cut(s) 762
BciVI GTATCC 1 cut(s) 499
BfaI CTAG 2 cut(s) 153, 197
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 2 cut(s) 464, 467
BlsI GCNGC 2 cut(s) 465, 468
Bme18I GGWCC 2 cut(s) 55, 486
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 55, 486, 702
BmiI GGNNCC 3 cut(s) 56, 203, 703
BmsI GCATC 2 cut(s) 282, 789
BpiI GAAGAC 1 cut(s) 580
Bpu10I CCTNAGC 1 cut(s) 316
BpuEI CTTGAG 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 82
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 318
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 2 cut(s) 115, 734
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 50, 318
BseMII CTCAG 4 cut(s) 330, 350, 425, 850
BseNI ACTGG 2 cut(s) 115, 734
BseRI GAGGAG 2 cut(s) 275, 278
BseSI GKGCMC 1 cut(s) 204
Bsh1236I CGCG 3 cut(s) 31, 350, 367
BshFI GGCC 1 cut(s) 704
BshNI GGYRCC 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 345, 440
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 50, 318
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 1 cut(s) 704
Bsp1286I GDGCHC 3 cut(s) 204, 345, 440
Bsp1407I TGTACA 1 cut(s) 717
Bsp143I GATC 4 cut(s) 216, 321, 376, 732
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 4 cut(s) 329, 349, 426, 849
BspFNI CGCG 3 cut(s) 31, 350, 367
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 56, 203, 703
BspPI GGATC 3 cut(s) 329, 384, 740
BspT107I GGYRCC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 717
BsrI ACTGG 2 cut(s) 115, 734
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 4 cut(s) 216, 321, 376, 732
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 3 cut(s) 132, 178, 184
BstAUI TGTACA 1 cut(s) 717
BstC8I GCNNGC 5 cut(s) 246, 250, 538, 778, 870
BstDEI CTNAG 4 cut(s) 316, 336, 434, 836
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 3 cut(s) 31, 350, 367
BstHHI GCGC 3 cut(s) 33, 43, 367
BstKTI GATC 4 cut(s) 219, 324, 379, 735
BstMBI GATC 4 cut(s) 216, 321, 376, 732
BstMWI GCNNNNNNNGC 4 cut(s) 96, 710, 809, 865
BstSLI GKGCMC 1 cut(s) 204
BstUI CGCG 3 cut(s) 31, 350, 367
BstV2I GAAGAC 1 cut(s) 580
BstX2I RGATCY 3 cut(s) 321, 376, 732
BstYI RGATCY 3 cut(s) 321, 376, 732
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 1 cut(s) 704
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 273
Cac8I GCNNGC 5 cut(s) 246, 250, 538, 778, 870
CciI TCATGA 1 cut(s) 562
CfoI GCGC 3 cut(s) 33, 43, 367
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 3 cut(s) 55, 486, 702
Csp6I GTAC 2 cut(s) 370, 718
CviAII CATG 2 cut(s) 229, 563
CviQI GTAC 2 cut(s) 370, 718
DdeI CTNAG 4 cut(s) 316, 336, 434, 836
DpnI GATC 4 cut(s) 218, 323, 378, 734
DpnII GATC 4 cut(s) 216, 321, 376, 732
DrdI GACNNNNNNGTC 1 cut(s) 115
DseDI GACNNNNNNGTC 1 cut(s) 115
Eam1104I CTCTTC 3 cut(s) 132, 178, 184
EarI CTCTTC 3 cut(s) 132, 178, 184
EciI GGCGGA 1 cut(s) 503
Ecl136II GAGCTC 2 cut(s) 343, 438
Eco24I GRGCYC 2 cut(s) 345, 440
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 55, 486
Eco53kI GAGCTC 2 cut(s) 343, 438
Eco57I CTGAAG 1 cut(s) 223
EcoICRI GAGCTC 2 cut(s) 343, 438
EcoO109I RGGNCCY 1 cut(s) 702
EcoRV GATATC 1 cut(s) 256
EcoT38I GRGCYC 2 cut(s) 345, 440
FaeI CATG 2 cut(s) 232, 566
FaiI YATR 7 cut(s) 230, 461, 564, 607, 687, 722, 810
FaqI GGGAC 1 cut(s) 68
FatI CATG 2 cut(s) 228, 562
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 2 cut(s) 464, 467
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 2 cut(s) 345, 440
Fsp4HI GCNGC 2 cut(s) 464, 467
FspBI CTAG 2 cut(s) 153, 197
GlaI GCGC 3 cut(s) 32, 42, 366
GluI GCNGC 2 cut(s) 464, 467
HaeIII GGCC 1 cut(s) 704
HapII CCGG 1 cut(s) 249
HhaI GCGC 3 cut(s) 33, 43, 367
Hin1II CATG 2 cut(s) 232, 566
Hin6I GCGC 3 cut(s) 31, 41, 365
HinP1I GCGC 3 cut(s) 31, 41, 365
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 1 cut(s) 334
HpaII CCGG 1 cut(s) 249
HphI GGTGA 3 cut(s) 111, 250, 490
Hpy166II GTNNAC 5 cut(s) 77, 384, 403, 573, 667
Hpy188I TCNGA 5 cut(s) 108, 216, 225, 435, 798
Hpy188III TCNNGA 4 cut(s) 331, 338, 563, 581
Hpy8I GTNNAC 5 cut(s) 77, 384, 403, 573, 667
HpyAV CCTTC 3 cut(s) 79, 247, 412
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 2 cut(s) 116, 509
HpyCH4V TGCA 5 cut(s) 623, 713, 780, 812, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 710, 809, 865
HpyF3I CTNAG 4 cut(s) 316, 336, 434, 836
HpySE526I ACGT 2 cut(s) 116, 509
Hsp92II CATG 2 cut(s) 232, 566
HspAI GCGC 3 cut(s) 31, 41, 365
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 4 cut(s) 216, 321, 376, 732
LmnI GCTCC 3 cut(s) 340, 499, 533
LweI GCATC 2 cut(s) 282, 789
MaeI CTAG 2 cut(s) 153, 197
MaeII ACGT 2 cut(s) 116, 509
MaeIII GTNAC 1 cut(s) 117
MalI GATC 4 cut(s) 218, 323, 378, 734
MbiI CCGCTC 1 cut(s) 476
MboI GATC 4 cut(s) 216, 321, 376, 732
MboII GAAGA 4 cut(s) 119, 165, 171, 580
MflI RGATCY 3 cut(s) 321, 376, 732
MhlI GDGCHC 3 cut(s) 204, 345, 440
MluCI AATT 5 cut(s) 158, 624, 635, 670, 758
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 7 cut(s) 33, 41, 50, 131, 248, 511, 821
MreI CGCCGGCG 1 cut(s) 248
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 2 cut(s) 861, 874
MspI CCGG 1 cut(s) 249
MvnI CGCG 3 cut(s) 31, 350, 367
MwoI GCNNNNNNNGC 4 cut(s) 96, 710, 809, 865
NaeI GCCGGC 1 cut(s) 250
NdeII GATC 4 cut(s) 216, 321, 376, 732
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 2 cut(s) 232, 566
NlaIV GGNNCC 3 cut(s) 56, 203, 703
NmuCI GTSAC 1 cut(s) 117
PagI TCATGA 1 cut(s) 562
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 465, 468
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
Psp124BI GAGCTC 2 cut(s) 345, 440
PspN4I GGNNCC 3 cut(s) 56, 203, 703
PspPI GGNCC 3 cut(s) 55, 486, 702
PsuI RGATCY 3 cut(s) 321, 376, 732
RsaI GTAC 2 cut(s) 371, 719
RsaNI GTAC 2 cut(s) 370, 718
SacI GAGCTC 2 cut(s) 345, 440
SaqAI TTAA 2 cut(s) 861, 874
SatI GCNGC 2 cut(s) 464, 467
Sau3AI GATC 4 cut(s) 216, 321, 376, 732
Sau96I GGNCC 3 cut(s) 55, 486, 702
SchI GAGTC 1 cut(s) 328
SduI GDGCHC 3 cut(s) 204, 345, 440
SfaNI GCATC 2 cut(s) 282, 789
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 2 cut(s) 55, 486
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 5 cut(s) 158, 624, 635, 670, 758
SspMI CTAG 2 cut(s) 153, 197
SstI GAGCTC 2 cut(s) 345, 440
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 2 cut(s) 119, 512
TaqI TCGA 1 cut(s) 766
TaqII GACCGA 1 cut(s) 388
TasI AATT 5 cut(s) 158, 624, 635, 670, 758
TatI WGTACW 1 cut(s) 717
TauI GCSGC 2 cut(s) 466, 469
Tru1I TTAA 2 cut(s) 861, 874
Tru9I TTAA 2 cut(s) 861, 874
TseFI GTSAC 1 cut(s) 117
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 7 cut(s) 17, 153, 245, 382, 418, 431, 551
TspGWI ACGGA 2 cut(s) 400, 401
VpaK11BI GGWCC 2 cut(s) 55, 486
XapI RAATTY 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 2 cut(s) 153, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.