pycom10g22930

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015807
Physical Location & Seq
Forward (+)
25218883 .. 25221152
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22930.3

Sequence Viewer

Length: 648 bp
ATGAATGTCTTCAGATTGACTGGCGACATGACTCACTTGGCCAGTATTCTGGTCCTCCTCCTCAAAATCTATGCGACAAAATCATGCTCAGGGATTTCGCTTAAGACTCAGGAGCTCTATGCGCTAGTGTTTCTGGCTCGGTACCTGAACCTGTTTACGGACTTTATTTCGGTGTATAACACTGTGATGAAGCTTGTGTTTATTGGAAGCTCGCTTGCCATTGTTTGGTGTATGCGGATGCACAAAATGGTGAAGCGTTCTTACGATCAAGAGCTCGATACATTCCGCCATTATTTTCTTATAGCAGGCTGCTTTTTGCTCGCCCTGCTTGTCAATGAGAAGTTCACGTTTCCAGAGGTCCTCTGGGCTTTTTCGATATACTTGGAGGCGGTTTCTATTATTCCGCAGTTAGTTTTGCTACAACGAAGCGGAAATGTGGACAATTTGACAGGGCAATATGTGTTCCTTCTTGGGGCCTATCGTGCATTGTACATTCTCAACTGGATCTACCGCTACTTCACAGAGCCACACTTCAGTCGATGGATTGCTTGTGTCGCCGGTCTGGTTCAGACAGCTTTATATGCAGATTTTTTCTACTACTACTTTCTCAGCTGGAAAAACAATGCGAAGCTTCAGTTGCCATCGTAA

Protein Analysis

216

Amino Acids

25.09

Weight (kDa)

9.1

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29330 AT1G29330
fragaria_vesca FvH4_3g07240 FvH4_3g07240
malus_domestica MD00G1008400.v1.1
prunus_persica Prupe.4G067600_v2.0.a1
pyrus_communis pycom05g27360 pycom10g22930
rosa_chinensis RchiOBHm_Chr5g0011901
rosa_laevigata RLG00000031866
rosa_multiflora Rmu_sc0001475.1_g000009
rosa_roxburghii Rroxscaffold_1G00064730
rosa_rugosa Rorug04G0459600
rosa_samantha Rh5CG100900
rosa_wichuraiana Rw5G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 225
AciI CCGC 6 cut(s) 235, 286, 389, 404, 429, 511
AclWI GGATC 1 cut(s) 512
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 2 cut(s) 517, 617
AfaI GTAC 2 cut(s) 143, 491
AfiI CCNNNNNNNGG 3 cut(s) 157, 225, 472
AflII CTTAAG 1 cut(s) 101
AluBI AGCT 7 cut(s) 115, 193, 210, 274, 575, 612, 631
AluI AGCT 7 cut(s) 115, 193, 210, 274, 575, 612, 631
Alw21I GWGCWC 2 cut(s) 117, 276
AlwI GGATC 1 cut(s) 512
AoxI GGCC 2 cut(s) 39, 474
ApeKI GCWGC 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 141
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 3 cut(s) 52, 358, 474
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 52, 358
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 141
BanII GRGCYC 2 cut(s) 117, 276
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 2 cut(s) 117, 276
BbvI GCAGC 1 cut(s) 296
BccI CCATC 1 cut(s) 534
BfaI CTAG 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 101
BisI GCNGC 1 cut(s) 310
BlsI GCNGC 1 cut(s) 311
Bme18I GGWCC 2 cut(s) 52, 358
BmgT120I GGNCC 3 cut(s) 52, 358, 474
BmiI GGNNCC 2 cut(s) 143, 475
BmsI GCATC 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 88
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 225, 472
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 25, 42, 506
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 157, 225, 472
BseMII CTCAG 3 cut(s) 102, 122, 622
BseNI ACTGG 3 cut(s) 25, 42, 506
BseRI GAGGAG 2 cut(s) 47, 50
BseXI GCAGC 1 cut(s) 296
BshFI GGCC 2 cut(s) 41, 476
BshNI GGYRCC 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 117, 276
BsiSI CCGG 1 cut(s) 558
BslI CCNNNNNNNGG 3 cut(s) 157, 225, 472
BsnI GGCC 2 cut(s) 41, 476
Bsp1286I GDGCHC 2 cut(s) 117, 276
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 2 cut(s) 265, 504
BspACI CCGC 6 cut(s) 235, 286, 389, 404, 429, 511
BspANI GGCC 2 cut(s) 41, 476
BspCNI CTCAG 3 cut(s) 101, 121, 621
BspLI GGNNCC 2 cut(s) 143, 475
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 557
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 3 cut(s) 25, 42, 506
BssAI RCCGGY 1 cut(s) 557
BssMI GATC 2 cut(s) 265, 504
Bst4CI ACNGT 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 101
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 212, 216, 307, 321
BstDEI CTNAG 3 cut(s) 88, 108, 608
BstF5I GGATG 1 cut(s) 243
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 2 cut(s) 268, 507
BstMBI GATC 2 cut(s) 265, 504
BstMWI GCNNNNNNNGC 6 cut(s) 121, 325, 482, 554, 581, 637
BstV1I GCAGC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 504
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 1 cut(s) 504
BsuRI GGCC 2 cut(s) 41, 476
BtsCI GGATG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 4 cut(s) 212, 216, 307, 321
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 3 cut(s) 52, 358, 474
Csp6I GTAC 2 cut(s) 142, 490
CviAII CATG 2 cut(s) 28, 84
CviQI GTAC 2 cut(s) 142, 490
DdeI CTNAG 3 cut(s) 88, 108, 608
DpnI GATC 2 cut(s) 267, 506
DpnII GATC 2 cut(s) 265, 504
EaeI YGGCCR 1 cut(s) 39
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 2 cut(s) 115, 274
Eco24I GRGCYC 2 cut(s) 117, 276
Eco47I GGWCC 2 cut(s) 52, 358
Eco53kI GAGCTC 2 cut(s) 115, 274
Eco57I CTGAAG 2 cut(s) 517, 617
EcoICRI GAGCTC 2 cut(s) 115, 274
EcoO109I RGGNCCY 2 cut(s) 358, 474
EcoT38I GRGCYC 2 cut(s) 117, 276
FaeI CATG 2 cut(s) 31, 87
FatI CATG 2 cut(s) 27, 83
Fnu4HI GCNGC 1 cut(s) 310
FokI GGATG 1 cut(s) 250
FriOI GRGCYC 2 cut(s) 117, 276
Fsp4HI GCNGC 1 cut(s) 310
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 123
GluI GCNGC 1 cut(s) 310
HaeIII GGCC 2 cut(s) 41, 476
HapII CCGG 1 cut(s) 558
HhaI GCGC 1 cut(s) 124
Hin1II CATG 2 cut(s) 31, 87
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HindIII AAGCTT 2 cut(s) 191, 629
HinfI GANTC 2 cut(s) 31, 106
HpaII CCGG 1 cut(s) 558
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 3 cut(s) 156, 345, 439
Hpy188I TCNGA 2 cut(s) 14, 570
Hpy188III TCNNGA 3 cut(s) 110, 269, 353
Hpy8I GTNNAC 3 cut(s) 156, 345, 439
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 3 cut(s) 241, 485, 584
HpyF10VI GCNNNNNNNGC 6 cut(s) 121, 325, 482, 554, 581, 637
HpyF3I CTNAG 3 cut(s) 88, 108, 608
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 2 cut(s) 31, 87
HspAI GCGC 1 cut(s) 122
KpnI GGTACC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 265, 504
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 1 cut(s) 228
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 347
MalI GATC 2 cut(s) 267, 506
MboI GATC 2 cut(s) 265, 504
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 2 cut(s) 117, 276
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 2 cut(s) 25, 100
MnlI CCTC 6 cut(s) 65, 68, 71, 349, 371, 379
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 612
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 1 cut(s) 558
MwoI GCNNNNNNNGC 6 cut(s) 121, 325, 482, 554, 581, 637
NdeII GATC 2 cut(s) 265, 504
NlaIII CATG 2 cut(s) 31, 87
NlaIV GGNNCC 2 cut(s) 143, 475
PdmI GAANNNNTTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 1 cut(s) 311
PleI GAGTC 2 cut(s) 25, 100
PpsI GAGTC 2 cut(s) 25, 100
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 2 cut(s) 117, 276
Psp5II RGGWCCY 1 cut(s) 358
PspN4I GGNNCC 2 cut(s) 143, 475
PspPI GGNCC 3 cut(s) 52, 358, 474
PspPPI RGGWCCY 1 cut(s) 358
PsuI RGATCY 1 cut(s) 504
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 2 cut(s) 143, 491
RsaNI GTAC 2 cut(s) 142, 490
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 2 cut(s) 117, 276
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 1 cut(s) 310
Sau3AI GATC 2 cut(s) 265, 504
Sau96I GGNCC 3 cut(s) 52, 358, 474
SchI GAGTC 2 cut(s) 25, 100
SduI GDGCHC 2 cut(s) 117, 276
SfaNI GCATC 1 cut(s) 228
SinI GGWCC 2 cut(s) 52, 358
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 1 cut(s) 442
SsiI CCGC 6 cut(s) 235, 286, 389, 404, 429, 511
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 2 cut(s) 117, 276
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 350
TaqI TCGA 3 cut(s) 276, 374, 538
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 489
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 187
TseI GCWGC 1 cut(s) 309
TspDTI ATGAA 2 cut(s) 17, 203
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 225
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 2 cut(s) 52, 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.