pycom05g27360

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015807
Physical Location & Seq
Forward (+)
28349266 .. 28351590
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27360.3

Sequence Viewer

Length: 648 bp
ATGAATGTCTTCAGATTGGCCGGCGACATGACTCACTTGGCCAGTATTCTGGTCCTCCTCCTCAAAATTTACGCCACCAAATCGTGCTCAGGAATTTCGCTTAAGACTCAGGAGCTCTATGCCCTGGTGTTTCTGGCTCGGTATTTGGACCTGTTTACGAACTTTGTTTCTGTGTATAACACTGTGATGAAGCTGGTGTTTATCGGAAGCTCGCTGGCCATTGTTTGGTGTATGCGTATGCACAAAATGGTGAAGCGTTCTTACAATAAAGAGCTCGATACGTTCCGGCATTATTTTCTTATTGCTGGCTGCTTTATGCTCGCCCTGCTTGTCAACGAGAAGTTCACGTTTCAAGAGGTGCTCTGGGCATTTTCTATATACTTGGAGGCGGTTTCTATTATTCCGCAGTTAATTTTGCTACAACGAAGTGGAAATGTGGACAATTTGACAGGGCAATATGTGTTCCTTCTTGGGGCCTATCGTGCATTGTACATTCTCAACTCGATCTACCGCTACTTCACAGAACCACACTTCAGTCGATGGATAGCATGTGTTGCCGGTCTGGTTCAGACAGCTTTATATGCAGATTTTTTCTACTACTATTTTCTCAGCTGGAAAAACAATGCAAAGCTTCAGCTGCCAGCATAA

Protein Analysis

216

Amino Acids

24.99

Weight (kDa)

9.3

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29330 AT1G29330
fragaria_vesca FvH4_3g07240 FvH4_3g07240
malus_domestica MD00G1008400.v1.1
prunus_persica Prupe.4G067600_v2.0.a1
pyrus_communis pycom05g27360 pycom10g22930
rosa_chinensis RchiOBHm_Chr5g0011901
rosa_laevigata RLG00000031866
rosa_multiflora Rmu_sc0001475.1_g000009
rosa_roxburghii Rroxscaffold_1G00064730
rosa_rugosa Rorug04G0459600
rosa_samantha Rh5CG100900
rosa_wichuraiana Rw5G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 225
AciI CCGC 3 cut(s) 389, 404, 511
AcoI YGGCCR 3 cut(s) 18, 39, 216
AcsI RAATTY 2 cut(s) 66, 93
AcuI CTGAAG 2 cut(s) 517, 617
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 2 cut(s) 225, 472
AflII CTTAAG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 353
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 8 cut(s) 115, 193, 210, 274, 575, 612, 631, 637
AluI AGCT 8 cut(s) 115, 193, 210, 274, 575, 612, 631, 637
Alw21I GWGCWC 4 cut(s) 89, 117, 276, 363
AoxI GGCC 4 cut(s) 18, 39, 216, 474
ApeKI GCWGC 2 cut(s) 309, 637
ApoI RAATTY 2 cut(s) 66, 93
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 3 cut(s) 52, 148, 474
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 52, 148
BalI TGGCCA 2 cut(s) 41, 218
BanII GRGCYC 2 cut(s) 117, 276
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 4 cut(s) 89, 117, 276, 363
BbvI GCAGC 2 cut(s) 296, 624
BccI CCATC 1 cut(s) 534
BciT130I CCWGG 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 101
BisI GCNGC 2 cut(s) 310, 638
BlsI GCNGC 2 cut(s) 311, 639
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 52, 148
BmgT120I GGNCC 3 cut(s) 52, 148, 474
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 472
Bse118I RCCGGY 2 cut(s) 20, 557
Bse1I ACTGG 1 cut(s) 42
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 225, 472
BseMII CTCAG 3 cut(s) 102, 122, 622
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 47, 50
BseXI GCAGC 2 cut(s) 296, 624
BshFI GGCC 4 cut(s) 20, 41, 218, 476
BsiHKAI GWGCWC 4 cut(s) 89, 117, 276, 363
BsiSI CCGG 3 cut(s) 21, 286, 558
BslI CCNNNNNNNGG 2 cut(s) 225, 472
BsnI GGCC 4 cut(s) 20, 41, 218, 476
Bsp1286I GDGCHC 4 cut(s) 89, 117, 276, 363
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 1 cut(s) 504
BspACI CCGC 3 cut(s) 389, 404, 511
BspANI GGCC 4 cut(s) 20, 41, 218, 476
BspCNI CTCAG 3 cut(s) 101, 121, 621
BspLI GGNNCC 1 cut(s) 475
BspTI CTTAAG 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 20, 557
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 1 cut(s) 42
BssAI RCCGGY 2 cut(s) 20, 557
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 101
BstAPI GCANNNNNTGC 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 6 cut(s) 22, 212, 216, 307, 321, 642
BstDEI CTNAG 3 cut(s) 88, 108, 608
BstKTI GATC 1 cut(s) 507
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 5 cut(s) 325, 482, 554, 581, 637
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 296, 624
BstXI CCANNNNNNTGG 1 cut(s) 49
BsuRI GGCC 4 cut(s) 20, 41, 218, 476
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 6 cut(s) 22, 212, 216, 307, 321, 642
Cfr10I RCCGGY 2 cut(s) 20, 557
Cfr13I GGNCC 3 cut(s) 52, 148, 474
Csp6I GTAC 1 cut(s) 490
CviAII CATG 2 cut(s) 28, 549
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 3 cut(s) 88, 108, 608
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
EaeI YGGCCR 3 cut(s) 18, 39, 216
Ecl136II GAGCTC 2 cut(s) 115, 274
Eco24I GRGCYC 2 cut(s) 117, 276
Eco47I GGWCC 2 cut(s) 52, 148
Eco53kI GAGCTC 2 cut(s) 115, 274
Eco57I CTGAAG 2 cut(s) 517, 617
EcoICRI GAGCTC 2 cut(s) 115, 274
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 117, 276
FaeI CATG 2 cut(s) 31, 552
FatI CATG 2 cut(s) 27, 548
Fnu4HI GCNGC 2 cut(s) 310, 638
FriOI GRGCYC 2 cut(s) 117, 276
Fsp4HI GCNGC 2 cut(s) 310, 638
GluI GCNGC 2 cut(s) 310, 638
HaeIII GGCC 4 cut(s) 20, 41, 218, 476
HapII CCGG 3 cut(s) 21, 286, 558
Hin1II CATG 2 cut(s) 31, 552
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 2 cut(s) 31, 106
HpaII CCGG 3 cut(s) 21, 286, 558
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 4 cut(s) 156, 334, 345, 439
Hpy188I TCNGA 3 cut(s) 14, 206, 570
Hpy188III TCNNGA 3 cut(s) 90, 110, 353
Hpy8I GTNNAC 4 cut(s) 156, 334, 345, 439
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 2 cut(s) 281, 347
HpyCH4V TGCA 4 cut(s) 241, 485, 584, 626
HpyF10VI GCNNNNNNNGC 5 cut(s) 325, 482, 554, 581, 637
HpyF3I CTNAG 3 cut(s) 88, 108, 608
HpySE526I ACGT 2 cut(s) 281, 347
Hsp92II CATG 2 cut(s) 31, 552
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 2 cut(s) 296, 624
MaeII ACGT 2 cut(s) 281, 347
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MhlI GDGCHC 4 cut(s) 89, 117, 276, 363
MlsI TGGCCA 2 cut(s) 41, 218
MluCI AATT 4 cut(s) 66, 93, 411, 442
MluNI TGGCCA 2 cut(s) 41, 218
MlyI GAGTC 2 cut(s) 25, 100
MnlI CCTC 5 cut(s) 65, 68, 71, 349, 379
Mox20I TGGCCA 2 cut(s) 41, 218
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 2 cut(s) 41, 218
MseI TTAA 2 cut(s) 102, 410
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 2 cut(s) 41, 218
MspA1I CMGCKG 2 cut(s) 612, 637
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 3 cut(s) 21, 286, 558
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 5 cut(s) 325, 482, 554, 581, 637
NaeI GCCGGC 1 cut(s) 22
NdeII GATC 1 cut(s) 504
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 31, 552
NlaIV GGNNCC 1 cut(s) 475
NspI RCATGY 1 cut(s) 552
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 2 cut(s) 311, 639
PleI GAGTC 2 cut(s) 25, 100
PpsI GAGTC 2 cut(s) 25, 100
Psp124BI GAGCTC 2 cut(s) 117, 276
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 3 cut(s) 52, 148, 474
PvuII CAGCTG 2 cut(s) 612, 637
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 2 cut(s) 117, 276
SaqAI TTAA 2 cut(s) 102, 410
SatI GCNGC 2 cut(s) 310, 638
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 3 cut(s) 52, 148, 474
SchI GAGTC 2 cut(s) 25, 100
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 4 cut(s) 89, 117, 276, 363
SinI GGWCC 2 cut(s) 52, 148
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 4 cut(s) 66, 93, 411, 442
SsiI CCGC 3 cut(s) 389, 404, 511
SstI GAGCTC 2 cut(s) 117, 276
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 2 cut(s) 284, 350
TaqI TCGA 3 cut(s) 276, 503, 538
TasI AATT 4 cut(s) 66, 93, 411, 442
TatI WGTACW 1 cut(s) 489
Tru1I TTAA 2 cut(s) 102, 410
Tru9I TTAA 2 cut(s) 102, 410
TscAI CASTG 1 cut(s) 187
TseI GCWGC 2 cut(s) 309, 637
TspDTI ATGAA 2 cut(s) 17, 203
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 225
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 2 cut(s) 52, 148
XapI RAATTY 2 cut(s) 66, 93
XceI RCATGY 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.