Rroxscaffold_1G00064730

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015807
Physical Location & Seq
Forward (+)
86525962 .. 86528656
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064730.1

Sequence Viewer

Length: 648 bp
ATGAACGCCTTCAGGCTCGCCGGCGATATCACCCACCTCATCAGCATCCTCGTCCTCCTCCTCAAAATCTACGCCACCAAATCCTGCTCCGGGATCTCGCTCAAGACTCAGGAGCTCTACGCGCTGGTGTTTCTGGCGCGGTACTTGGATCTGTTCACGGATTTCATTTCGGTCTACAACTCCGTGATGAAGGTGGTGTTCATTCTGAGCTCGGTGGCGATTGTGTGGTGTATGCGGCGGCACAGAGTCGTGAAGCGGTCCTACGATAAGGAGCGGGATACGTTCCGCCATTACTATCTCGTCGGAGCTTGCTTTGTGTTTGCTCTGCTTGTTCATGAGAAGTTCACATTTCAGGAGGTCTTCTGGGCATTTTCAATATACTTGGAGGCAGTTGCAATTATTCCGCAATTAGTTTTGCTGCAACGAAGTGGAAATGTGGACAATTTGACAGGGCAGTATGTTTTCTTTCTGGGGGCCTATCGTGCATTGTACATACTCAACTGGATCTACCGCTACTTCACAGAAGCAAAATTCAGTCGATGGATAGCTTGCATCTCTGGTCTGGTTCAGACAGCTTTGTATGCAGATTTTTTCTACTACTACTTTCTCAGTTGGAAAAACAATGCAAAGCTCAACCTGCCAGCTTAA

Protein Analysis

215

Amino Acids

25.23

Weight (kDa)

9.37

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 28 - 171 9.1e-60 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29330 AT1G29330
fragaria_vesca FvH4_3g07240 FvH4_3g07240
malus_domestica MD00G1008400.v1.1
prunus_persica Prupe.4G067600_v2.0.a1
pyrus_communis pycom05g27360 pycom10g22930
rosa_chinensis RchiOBHm_Chr5g0011901
rosa_laevigata RLG00000031866
rosa_multiflora Rmu_sc0001475.1_g000009
rosa_roxburghii Rroxscaffold_1G00064730
rosa_rugosa Rorug04G0459600
rosa_samantha Rh5CG100900
rosa_wichuraiana Rw5G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 174
AccII CGCG 2 cut(s) 122, 139
AciI CCGC 8 cut(s) 139, 235, 238, 256, 274, 286, 404, 511
AclWI GGATC 3 cut(s) 101, 156, 512
AcsI RAATTY 1 cut(s) 530
AfaI GTAC 2 cut(s) 143, 491
AfiI CCNNNNNNNGG 1 cut(s) 90
AgsI TTSAA 1 cut(s) 375
AluBI AGCT 7 cut(s) 115, 210, 308, 548, 575, 631, 644
AluI AGCT 7 cut(s) 115, 210, 308, 548, 575, 631, 644
Alw21I GWGCWC 2 cut(s) 117, 212
AlwI GGATC 3 cut(s) 101, 156, 512
AoxI GGCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 418
ApoI RAATTY 1 cut(s) 530
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 124, 139
AspS9I GGNCC 2 cut(s) 258, 474
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 22
AvaII GGWCC 1 cut(s) 258
BanII GRGCYC 2 cut(s) 117, 212
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BbsI GAAGAC 1 cut(s) 352
Bbv12I GWGCWC 2 cut(s) 117, 212
BbvI GCAGC 1 cut(s) 405
BccI CCATC 1 cut(s) 534
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 91
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 3 cut(s) 236, 239, 419
BlsI GCNGC 3 cut(s) 237, 240, 420
Bme1390I CCNGG 1 cut(s) 91
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 258, 474
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 2 cut(s) 54, 561
BpiI GAAGAC 1 cut(s) 352
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 1 cut(s) 90
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 506
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 90
BseMII CTCAG 3 cut(s) 122, 197, 622
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 2 cut(s) 47, 50
BseXI GCAGC 1 cut(s) 405
Bsh1236I CGCG 2 cut(s) 122, 139
BshFI GGCC 1 cut(s) 476
BsiHKAI GWGCWC 2 cut(s) 117, 212
BsiSI CCGG 2 cut(s) 21, 90
BslI CCNNNNNNNGG 1 cut(s) 90
BsnI GGCC 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 117, 212
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 3 cut(s) 93, 148, 504
BspACI CCGC 8 cut(s) 139, 235, 238, 256, 274, 286, 404, 511
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 3 cut(s) 121, 198, 621
BspFNI CGCG 2 cut(s) 122, 139
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 3 cut(s) 101, 156, 512
BsrBI CCGCTC 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 20
BssMI GATC 3 cut(s) 93, 148, 504
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 5 cut(s) 18, 22, 310, 550, 642
BstDEI CTNAG 3 cut(s) 108, 206, 608
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 2 cut(s) 122, 139
BstHHI GCGC 2 cut(s) 124, 139
BstKTI GATC 3 cut(s) 96, 151, 507
BstMBI GATC 3 cut(s) 93, 148, 504
BstMWI GCNNNNNNNGC 4 cut(s) 121, 482, 581, 637
BstSCI CCNGG 1 cut(s) 89
BstUI CGCG 2 cut(s) 122, 139
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 3 cut(s) 93, 148, 504
BstYI RGATCY 3 cut(s) 93, 148, 504
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 45
Cac8I GCNNGC 5 cut(s) 18, 22, 310, 550, 642
CciI TCATGA 1 cut(s) 334
CfoI GCGC 2 cut(s) 124, 139
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 258, 474
Csp6I GTAC 2 cut(s) 142, 490
CviAII CATG 1 cut(s) 335
CviJI RGCY 9 cut(s) 16, 115, 210, 308, 476, 548, 575, 631, 644
CviKI_1 RGCY 9 cut(s) 16, 115, 210, 308, 476, 548, 575, 631, 644
CviQI GTAC 2 cut(s) 142, 490
DdeI CTNAG 3 cut(s) 108, 206, 608
DpnI GATC 3 cut(s) 95, 150, 506
DpnII GATC 3 cut(s) 93, 148, 504
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 2 cut(s) 115, 210
Eco24I GRGCYC 2 cut(s) 117, 212
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 2 cut(s) 115, 210
EcoICRI GAGCTC 2 cut(s) 115, 210
EcoO109I RGGNCCY 1 cut(s) 474
EcoRV GATATC 1 cut(s) 28
EcoT38I GRGCYC 2 cut(s) 117, 212
FaeI CATG 1 cut(s) 338
FaiI YATR 6 cut(s) 233, 336, 379, 459, 494, 582
FatI CATG 1 cut(s) 334
FauI CCCGC 1 cut(s) 267
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 236, 239, 419
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 2 cut(s) 117, 212
Fsp4HI GCNGC 3 cut(s) 236, 239, 419
GlaI GCGC 2 cut(s) 123, 138
GluI GCNGC 3 cut(s) 236, 239, 419
HaeIII GGCC 1 cut(s) 476
HapII CCGG 2 cut(s) 21, 90
HhaI GCGC 2 cut(s) 124, 139
Hin1II CATG 1 cut(s) 338
Hin6I GCGC 2 cut(s) 122, 137
HinP1I GCGC 2 cut(s) 122, 137
HinfI GANTC 2 cut(s) 106, 246
HpaII CCGG 2 cut(s) 21, 90
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 4 cut(s) 156, 175, 345, 439
Hpy188I TCNGA 3 cut(s) 207, 305, 570
Hpy188III TCNNGA 5 cut(s) 103, 110, 250, 335, 353
Hpy8I GTNNAC 4 cut(s) 156, 175, 345, 439
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 19, 184
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 6 cut(s) 395, 421, 485, 552, 584, 626
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 482, 581, 637
HpyF3I CTNAG 3 cut(s) 108, 206, 608
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 1 cut(s) 338
HspAI GCGC 2 cut(s) 122, 137
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 3 cut(s) 93, 148, 504
LmnI GCTCC 4 cut(s) 92, 112, 271, 305
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 2 cut(s) 54, 561
MaeII ACGT 1 cut(s) 281
MalI GATC 3 cut(s) 95, 150, 506
MbiI CCGCTC 1 cut(s) 274
MboI GATC 3 cut(s) 93, 148, 504
MboII GAAGA 1 cut(s) 352
MflI RGATCY 3 cut(s) 93, 148, 504
MhlI GDGCHC 2 cut(s) 117, 212
MluCI AATT 4 cut(s) 396, 407, 442, 530
MlyI GAGTC 2 cut(s) 100, 255
MmeI TCCRAC 2 cut(s) 283, 593
MnlI CCTC 7 cut(s) 47, 59, 65, 68, 71, 349, 379
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 646
MspI CCGG 2 cut(s) 21, 90
MspR9I CCNGG 1 cut(s) 91
MvnI CGCG 2 cut(s) 122, 139
MwoI GCNNNNNNNGC 4 cut(s) 121, 482, 581, 637
NaeI GCCGGC 1 cut(s) 22
NciI CCSGG 1 cut(s) 91
NdeII GATC 3 cut(s) 93, 148, 504
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 338
NlaIV GGNNCC 1 cut(s) 475
PagI TCATGA 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 3 cut(s) 237, 240, 420
PleI GAGTC 2 cut(s) 100, 254
PpsI GAGTC 2 cut(s) 100, 254
Psp124BI GAGCTC 2 cut(s) 117, 212
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 2 cut(s) 258, 474
PsuI RGATCY 3 cut(s) 93, 148, 504
RsaI GTAC 2 cut(s) 143, 491
RsaNI GTAC 2 cut(s) 142, 490
SacI GAGCTC 2 cut(s) 117, 212
SaqAI TTAA 1 cut(s) 646
SatI GCNGC 3 cut(s) 236, 239, 419
Sau3AI GATC 3 cut(s) 93, 148, 504
Sau96I GGNCC 2 cut(s) 258, 474
SchI GAGTC 2 cut(s) 100, 255
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 2 cut(s) 117, 212
SfaNI GCATC 2 cut(s) 54, 561
SgrAI CRCCGGYG 1 cut(s) 20
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 4 cut(s) 396, 407, 442, 530
SsiI CCGC 8 cut(s) 139, 235, 238, 256, 274, 286, 404, 511
SstI GAGCTC 2 cut(s) 117, 212
StyD4I CCNGG 1 cut(s) 89
TaiI ACGT 1 cut(s) 284
TaqI TCGA 1 cut(s) 538
TaqII GACCGA 1 cut(s) 160
TasI AATT 4 cut(s) 396, 407, 442, 530
TatI WGTACW 1 cut(s) 489
TauI GCSGC 2 cut(s) 238, 241
Tru1I TTAA 1 cut(s) 646
Tru9I TTAA 1 cut(s) 646
TseI GCWGC 1 cut(s) 418
TspDTI ATGAA 5 cut(s) 17, 154, 190, 203, 323
TspGWI ACGGA 2 cut(s) 172, 173
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.