RLG00000031866

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015807
Physical Location & Seq
Reverse (-)
8883373 .. 8886059
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031866

Sequence Viewer

Length: 648 bp
ATGAACGCCTTCAGGCTCGCCGGCGATATCACCCACCTCATCAGCATCCTCGTCCTCCTCCTCAAAATCTACGCCACCAAATCCTGCTCAGGGATCTCGCTCAAGACTCAGGAGCTCTACGCGGTGGTGTTTCTGGCCCGGTACTTGGATCTGTTCACGGATTTCATTTCGGTGTACAACTCCGTGATGAAGGTGGTGTTCATTCTGAGCTCGGTGGCGATTGTGTGGTGTATGCGGCGGCACAGAGTGGTGAAGCGGTCCTATGATAAGGAGCTGGATACGTTCCGCCATTACTATCTCGTTGGAGCTTGCTTTGTGTTTGCTCTGCTTGTTCATGAGAAGTTCACATTTCAGGAGGTCTTCTGGGCATTTTCAATATACTTGGAGGCAGTTGCAATTATTCCGCAATTAGTTTTGCTACAACGAAGTGGAAATGTGGACAATTTGACAGGGCAGTATGTTTTCTTTCTGGGGGCCTATCGTGCATTGTACATACTCAACTGGATCTACCGCTACTTCACAGAAGCAAAATTCAGTCGATGGATAGCTTGCGTCTCTGGTCTGGTTCAGACAGCTTTGTATGCAGATTTTTTTTACTACTACTTTCTCAGTTGGAAAAACAATGCAAAGCTTAACCTGCCAGCTTAA

Protein Analysis

216

Amino Acids

25.16

Weight (kDa)

9.28

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 28 - 171 1.3e-59 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29330 AT1G29330
fragaria_vesca FvH4_3g07240 FvH4_3g07240
malus_domestica MD00G1008400.v1.1
prunus_persica Prupe.4G067600_v2.0.a1
pyrus_communis pycom05g27360 pycom10g22930
rosa_chinensis RchiOBHm_Chr5g0011901
rosa_laevigata RLG00000031866
rosa_multiflora Rmu_sc0001475.1_g000009
rosa_roxburghii Rroxscaffold_1G00064730
rosa_rugosa Rorug04G0459600
rosa_samantha Rh5CG100900
rosa_wichuraiana Rw5G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 122
AciI CCGC 7 cut(s) 122, 235, 238, 256, 286, 404, 511
AclWI GGATC 3 cut(s) 101, 156, 512
AcsI RAATTY 1 cut(s) 530
AdeI CACNNNGTG 1 cut(s) 247
AfaI GTAC 3 cut(s) 143, 176, 491
AfiI CCNNNNNNNGG 2 cut(s) 90, 145
AgsI TTSAA 1 cut(s) 375
AluBI AGCT 8 cut(s) 115, 210, 274, 308, 548, 575, 631, 644
AluI AGCT 8 cut(s) 115, 210, 274, 308, 548, 575, 631, 644
Alw21I GWGCWC 2 cut(s) 117, 212
Alw26I GTCTC 1 cut(s) 559
AlwI GGATC 3 cut(s) 101, 156, 512
AoxI GGCC 2 cut(s) 135, 474
ApoI RAATTY 1 cut(s) 530
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 3 cut(s) 136, 258, 474
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 22, 262
AvaII GGWCC 1 cut(s) 258
BanII GRGCYC 2 cut(s) 117, 212
BbsI GAAGAC 1 cut(s) 352
Bbv12I GWGCWC 2 cut(s) 117, 212
BccI CCATC 1 cut(s) 534
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 559
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 2 cut(s) 236, 239
BlsI GCNGC 2 cut(s) 237, 240
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 3 cut(s) 136, 258, 474
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 352
Bpu10I CCTNAGC 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 145
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 506
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 90, 145
BseMII CTCAG 4 cut(s) 102, 122, 197, 622
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 2 cut(s) 47, 50
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 2 cut(s) 137, 476
BsiHKAI GWGCWC 2 cut(s) 117, 212
BsiSI CCGG 2 cut(s) 21, 139
BslI CCNNNNNNNGG 2 cut(s) 90, 145
BsmAI GTCTC 1 cut(s) 559
BsmBI CGTCTC 1 cut(s) 559
BsnI GGCC 2 cut(s) 137, 476
Bsp1286I GDGCHC 2 cut(s) 117, 212
Bsp1407I TGTACA 2 cut(s) 174, 489
Bsp143I GATC 3 cut(s) 93, 148, 504
BspACI CCGC 7 cut(s) 122, 235, 238, 256, 286, 404, 511
BspANI GGCC 2 cut(s) 137, 476
BspCNI CTCAG 4 cut(s) 101, 121, 198, 621
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 3 cut(s) 101, 156, 512
BsrFI RCCGGY 1 cut(s) 20
BsrGI TGTACA 2 cut(s) 174, 489
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 20
BssMI GATC 3 cut(s) 93, 148, 504
BstAUI TGTACA 2 cut(s) 174, 489
BstC8I GCNNGC 5 cut(s) 18, 22, 310, 550, 642
BstDEI CTNAG 4 cut(s) 88, 108, 206, 608
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 3 cut(s) 96, 151, 507
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 3 cut(s) 93, 148, 504
BstMWI GCNNNNNNNGC 3 cut(s) 482, 581, 637
BstSCI CCNGG 1 cut(s) 137
BstUI CGCG 1 cut(s) 122
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 3 cut(s) 93, 148, 504
BstYI RGATCY 3 cut(s) 93, 148, 504
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 2 cut(s) 137, 476
BtsCI GGATG 1 cut(s) 45
Cac8I GCNNGC 5 cut(s) 18, 22, 310, 550, 642
CciI TCATGA 1 cut(s) 334
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 3 cut(s) 136, 258, 474
CseI GACGC 1 cut(s) 541
Csp6I GTAC 3 cut(s) 142, 175, 490
CviAII CATG 1 cut(s) 335
CviQI GTAC 3 cut(s) 142, 175, 490
DdeI CTNAG 4 cut(s) 88, 108, 206, 608
DpnI GATC 3 cut(s) 95, 150, 506
DpnII GATC 3 cut(s) 93, 148, 504
DraIII CACNNNGTG 1 cut(s) 247
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 2 cut(s) 115, 210
Eco24I GRGCYC 2 cut(s) 117, 212
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 2 cut(s) 115, 210
EcoICRI GAGCTC 2 cut(s) 115, 210
EcoO109I RGGNCCY 1 cut(s) 474
EcoRV GATATC 1 cut(s) 28
EcoT38I GRGCYC 2 cut(s) 117, 212
Esp3I CGTCTC 1 cut(s) 559
FaeI CATG 1 cut(s) 338
FaiI YATR 7 cut(s) 233, 264, 336, 379, 459, 494, 582
FatI CATG 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 236, 239
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 2 cut(s) 117, 212
Fsp4HI GCNGC 2 cut(s) 236, 239
GluI GCNGC 2 cut(s) 236, 239
HaeIII GGCC 2 cut(s) 137, 476
HapII CCGG 2 cut(s) 21, 139
HgaI GACGC 1 cut(s) 541
Hin1II CATG 1 cut(s) 338
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 1 cut(s) 106
HpaII CCGG 2 cut(s) 21, 139
HphI GGTGA 2 cut(s) 22, 262
Hpy166II GTNNAC 4 cut(s) 156, 175, 345, 439
Hpy188I TCNGA 2 cut(s) 207, 570
Hpy188III TCNNGA 4 cut(s) 103, 110, 335, 353
Hpy8I GTNNAC 4 cut(s) 156, 175, 345, 439
HpyAV CCTTC 2 cut(s) 19, 184
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 4 cut(s) 395, 485, 584, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 482, 581, 637
HpyF3I CTNAG 4 cut(s) 88, 108, 206, 608
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 1 cut(s) 338
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 3 cut(s) 93, 148, 504
LmnI GCTCC 3 cut(s) 112, 271, 305
LweI GCATC 1 cut(s) 54
MaeII ACGT 1 cut(s) 281
MalI GATC 3 cut(s) 95, 150, 506
MboI GATC 3 cut(s) 93, 148, 504
MboII GAAGA 1 cut(s) 352
MflI RGATCY 3 cut(s) 93, 148, 504
MhlI GDGCHC 2 cut(s) 117, 212
MluCI AATT 4 cut(s) 396, 407, 442, 530
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 2 cut(s) 283, 593
MnlI CCTC 7 cut(s) 47, 59, 65, 68, 71, 349, 379
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 633, 646
MslI CAYNNNNRTG 1 cut(s) 170
MspI CCGG 2 cut(s) 21, 139
MspR9I CCNGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 482, 581, 637
NaeI GCCGGC 1 cut(s) 22
NciI CCSGG 1 cut(s) 139
NdeII GATC 3 cut(s) 93, 148, 504
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 338
NlaIV GGNNCC 1 cut(s) 475
PagI TCATGA 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 2 cut(s) 237, 240
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
Psp124BI GAGCTC 2 cut(s) 117, 212
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 3 cut(s) 136, 258, 474
PsuI RGATCY 3 cut(s) 93, 148, 504
RsaI GTAC 3 cut(s) 143, 176, 491
RsaNI GTAC 3 cut(s) 142, 175, 490
RseI CAYNNNNRTG 1 cut(s) 170
SacI GAGCTC 2 cut(s) 117, 212
SaqAI TTAA 2 cut(s) 633, 646
SatI GCNGC 2 cut(s) 236, 239
Sau3AI GATC 3 cut(s) 93, 148, 504
Sau96I GGNCC 3 cut(s) 136, 258, 474
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 2 cut(s) 117, 212
SfaNI GCATC 1 cut(s) 54
SgrAI CRCCGGYG 1 cut(s) 20
SinI GGWCC 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 4 cut(s) 396, 407, 442, 530
SsiI CCGC 7 cut(s) 122, 235, 238, 256, 286, 404, 511
SstI GAGCTC 2 cut(s) 117, 212
StyD4I CCNGG 1 cut(s) 137
TaiI ACGT 1 cut(s) 284
TaqI TCGA 1 cut(s) 538
TasI AATT 4 cut(s) 396, 407, 442, 530
TatI WGTACW 2 cut(s) 174, 489
TauI GCSGC 2 cut(s) 238, 241
Tru1I TTAA 2 cut(s) 633, 646
Tru9I TTAA 2 cut(s) 633, 646
TspDTI ATGAA 5 cut(s) 17, 154, 190, 203, 323
TspGWI ACGGA 2 cut(s) 172, 173
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 1 cut(s) 530
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.