Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006301

Physical Location & Seq

Genomic Coordinates Reverse (-)

2513369 .. 2519863

Gene Structure 6495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1017400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGACGTGTGAAGCGGCGGTAATCGACGGCGGAAATGACCCGAGCAGCCAGAAATCTACCAACCAAGATCGGGTGAGGAAGACCCAGAAGCGAAAGAGGGCTAGCTTGGACCCAGAGCGTTTGGGAGTTGAGGCGAAAAGTATAAAGGTTCAATCTTTTCGCAAGCAGCTTGATGGGTTGTTTGGGTACTACAAGGAAGTGATGGGTCAGAAATTAGATTTAGATCCGAAGCAATGTGTGAACAATGTGAACTCGATCATTGGGGCTTTGATCGAAGAGAGTGGGCTGCCATTGTCCAAGCTTGTTGAGGAGGTCTTTGACAAGGTCAAGAACCGAAATGAGGTTTTTGGGAATGTGACTTTGGCCTATGTGAAGAGCATTGTTCTCTTTGTTGGGCAAAGAGTGATGTATGGCGTACCGAATGTTGATGCTGATGTGTTGGAGGACGAGTCCGAGTCCTGTCTCTGGTGTTGGGAGACAAGAGATGTAAAGTTGATGCCAGCATCTGTGCGAGGAGTATTGAATATTAGGCGTACTTGTCGAAGAAAGATCCATGAGAGGATTACTGCTGTTTCTGGAATGACAATGGCACTACAAAATCCCGAGAACGACCAAAATTACATACATTACCTGATGAAGGCATCAGAGCAGCTTGATAAAGCGCTGTCGGAGGAAAAGATTCGCTCGTTAATAGATCGCTTATCAGTGAAAAATGGTGCAGACGTGGCAAAGAAGGAAGCAAAAAGAGAAGAAAAACTACTAATTAAACAAATGGAAAGAGATAAACGAGAGGCTGAGAAAGAGAAAAAAAGATTGGAACGTGAGCGGCAAAAGGAAGAGTTGCAAAGTGAGAAACAGTTAAAGCGGCTGCAAGGGGAGTCCGAAAAAGATGAAAAGCGTCGTGAAAAAGAAGAGTCTGAAATGAGGAAACTGCAGAGGAAGCAGCAAGAGGATGCTGAAAGAGAACAACGCCGTCGAGAAAAGGAAGAAGCTGAATTGAAAAAAAAACTTTCCATAAAAAAGCAGGCTTCAATTATGGAACGATTTGTCAAAAGAAGCAAACCGATCGTAGCATGCCAGAATGACCAATTCCTAACTAAAGCAATTGTCTCCGAATCATTGGGTAAAAACAGTGAAAGTATGCCTGATGTAGTAACTCAGTCAATGGATCACACTCTTTCATCTAATATGGAGATTAGTGCTGACGATATACGCAGGTCACACTTGTCTTCTTGGTGCCACATTGGTTATGTTATTCGTTCAAACAGAAGCCAACACTGGGGCCGACGTCAGAAGCCTAAGACTGAACTATTTAAGGAATTGAAGCTCACAACCAGTAAAGAGCTAGTTCATGGTGATGATTTAAACACGGAGAAACTTGCGGATAGATGGGGAGAAGAAGTTTGCAATGATAAATCATGTCAGACTAATACAGATTGTTCTCTTGCTGATGTTAAAAGATGCAAGCTGGGAAAGCAGTTGTTTCAATTTGATAAAAGCTGTAGACCTGCATTTTATGGTATATGGCCTAAGAAAAGTCATGTTGTTGGACCATGCCACCCTTTTAGGAAGGACCCAGACTTGGATTATGACGTTGACAGCGATGAGGAGTGGGAAGAGGAGCCTGGAGAAAACCTCTCAGATTGCGATAAAGATGACGAAGAAGAAAGCTTGGAAGAGGGTTGTTCTAAACCTGATGATGAAGATGAAAGTGAAGATGACTTTTTTGTACCTGATGGGTATCTCTCAGAAAATGAGGGTGTACAGGTTGACAGGATGGAAACTGATACAACATTTGAGGAGACCAGAAGCTCACTCAGTATTAAGCAAGATTTAGAGAGTGAAAAATTCTCTATATTGCTTCGACAGCAAAAATATTTAGGGAATTTGACAGAACGTGCTCTTCAGAAAAATCAGCCTTTAATTATATCAAATATGGCGCATGACAAGGTCTCTTTGTTAAAGGCTGACGATCTTAATGGTACTCTTAAGCTGGAACAGATGTGCTTGCAAGCTCTGAGTATTCATGTATTTCCTGGTAGGTCACCTGTGGAGATATCAGTTGATGGCATACAAGAGGACGACCAAGAAGTTTGTCTTTCAATTGGCAATCGCTGTAGTAAATCAACCTCTTCCGTGACAGTTATACCAGAATCAGATTTGCCTGCAATAGTGTCCGTCATTCAGTCATGCTCACAGAGCATCAATAAGGTGTTACAAACTTTGCAACACAATTTCCCAGCTGTATCGAAGTCTCAGTTAAGGAATAAAGTGCGAGAGATATCAAATTTTGTGGATAGCCGCTGGCAGGTGAAGAAAGAAATTTTGGACAAAGTTGGCTTGTCAATATCTCCTGGTATTTGTGACTCTGTTCAGATGGAAAAGAGTGCTGGGCGGAGCAAGAATATAGCTACCTTTTTCTCGAAAAGGTGCTTGCCGCCTACTGGCAAAAGCTTTACTCCTAATGAAAATTCACCTCAGGTAGCCGGGAAGGCAAGTTCTGTGGAAGGGGATCAGAGTTGCACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006338 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008283 GO:0009555 GO:0009888 GO:0009934 GO:0009987 GO:0010026 GO:0016043 GO:0022603 GO:0022607 GO:0030154 GO:0031497 GO:0031507 GO:0032501 GO:0032502 GO:0032991 GO:0033186 GO:0033554 GO:0034622 GO:0034641 GO:0034728 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0045787 GO:0046483 GO:0048229 GO:0048366 GO:0048367 GO:0048509 GO:0048518 GO:0048522 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0090304 GO:0090558 GO:0099402 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CAF1A

Protein Analysis

843

Amino Acids

95.98

Weight (kDa)

5.92

Isoelectric Point (pI)

59.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1A_dimeriz	PF12253	494 - 558	1.4e-19	Chromatin assembly factor 1 subunit A dimerization domain
Cac1_C	PF21796	721 - 771	3.2e-20	Chromatin assembly factor 1 subunit Cac1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65470 AT1G65470
fragaria_vesca	FvH4_6g00081 FvH4_6g00081 FvH4_6g00081 FvH4_6g00081 FvH4_6g00081 FvH4_6g00081
malus_domestica	MD00G1017400.v1.1 MD12G1261700.v1.1
prunus_persica	Prupe.6G365000_v2.0.a1 Prupe.6G365000_v2.0.a1
pyrus_communis	pycom04g21970 pycom12g24210
rosa_chinensis	RchiOBHm_Chr3g0447421
rosa_laevigata	RLG00000026000
rosa_multiflora	Rmu_ssc0000473.1_g000021
rosa_roxburghii	Rroxscaffold_6G00425620
rosa_rugosa	Rorug02G0601300 Rorug02G0601400 Rorug02G0601500 Rorug02G0601600
rosa_samantha	Rh3AG001500 Rh3BG001200 Rh3CG001200 Rh3DG001400
rosa_wichuraiana	Rw3G000090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2299
AatII	GACGTC	1 cut(s)	1291
Acc36I	ACCTGC	3 cut(s)	1206, 1516, 2299
AccB1I	GGYRCC	1 cut(s)	1236
AccBSI	CCGCTC	1 cut(s)	826
AccI	GTMKAC	1 cut(s)	1504
AciI	CCGC	9 cut(s)	14, 17, 30, 826, 865, 1382, 2302, 2395, 2438
AclWI	GGATC	4 cut(s)	218, 544, 1176, 2520
AcsI	RAATTY	5 cut(s)	1847, 1885, 2287, 2322, 2470
AcuI	CTGAAG	1 cut(s)	1889
AcyI	GRCGYC	1 cut(s)	1288
AfaI	GTAC	6 cut(s)	188, 417, 535, 1731, 1764, 1984
AfeI	AGCGCT	1 cut(s)	663
AfiI	CCNNNNNNNGG	8 cut(s)	70, 340, 465, 637, 1279, 1582, 2308, 2444
AflII	CTTAAG	1 cut(s)	1988
AflIII	ACRYGT	1 cut(s)	5
AgsI	TTSAA	8 cut(s)	152, 523, 1000, 1032, 1263, 1324, 1487, 2103
AjiI	CACGTC	2 cut(s)	6, 724
AjnI	CCWGG	3 cut(s)	1624, 2035, 2353
AjuI	GAANNNNNNNTTGG	6 cut(s)	344, 376, 797, 829, 2309, 2341
AloI	GAACNNNNNNTCC	2 cut(s)	2482, 2514
Alw21I	GWGCWC	1 cut(s)	1903
Alw26I	GTCTC	6 cut(s)	467, 470, 1114, 1796, 1957, 2259
AlwI	GGATC	4 cut(s)	218, 544, 1176, 2520
AlwNI	CAGNNNCTG	1 cut(s)	506
Ama87I	CYCGRG	2 cut(s)	40, 602
Aor51HI	AGCGCT	1 cut(s)	663
AoxI	GGCC	3 cut(s)	363, 1282, 1526
ApeKI	GCWGC	6 cut(s)	45, 166, 286, 649, 868, 943
ApoI	RAATTY	5 cut(s)	1847, 1885, 2287, 2322, 2470
Asp700I	GAANNNNTTC	1 cut(s)	678
AspLEI	GCGC	2 cut(s)	664, 1942
AspS9I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
AsuC2I	CCSGG	1 cut(s)	2488
AsuHPI	GGTGA	5 cut(s)	85, 1367, 2037, 2323, 2466
AsuNHI	GCTAGC	1 cut(s)	101
AvaI	CYCGRG	2 cut(s)	40, 602
AvaII	GGWCC	3 cut(s)	109, 1550, 1573
AxyI	CCTNAGG	1 cut(s)	2478
BanI	GGYRCC	1 cut(s)	1236
BarI	GAAGNNNNNNTAC	2 cut(s)	741, 773
BbsI	GAAGAC	2 cut(s)	86, 1221
Bbv12I	GWGCWC	1 cut(s)	1903
BbvI	GCAGC	6 cut(s)	57, 178, 273, 661, 855, 955
BccI	CCATC	7 cut(s)	167, 196, 1383, 1730, 1771, 2060, 2371
BceAI	ACGGC	2 cut(s)	43, 957
BcgI	CGANNNNNNTGC	2 cut(s)	1048, 1082
BciT130I	CCWGG	3 cut(s)	1626, 2037, 2355
BcnI	CCSGG	1 cut(s)	2488
BcoDI	GTCTC	6 cut(s)	467, 470, 1114, 1796, 1957, 2259
BfaI	CTAG	2 cut(s)	102, 1346
BfmI	CTRYAG	3 cut(s)	932, 1501, 2116
BfoI	RGCGCY	1 cut(s)	665
BfrI	CTTAAG	1 cut(s)	1988
BfuAI	ACCTGC	3 cut(s)	1206, 1516, 2299
BglI	GCCNNNNNGGC	1 cut(s)	2492
Bme1390I	CCNGG	4 cut(s)	1626, 2037, 2355, 2488
Bme18I	GGWCC	3 cut(s)	109, 1550, 1573
BmeT110I	CYCGRG	2 cut(s)	40, 602
BmgBI	CACGTC	2 cut(s)	6, 724
BmgT120I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
BmiI	GGNNCC	5 cut(s)	111, 1238, 1283, 1575, 1623
BmrFI	CCNGG	4 cut(s)	1626, 2037, 2355, 2488
BmrI	ACTGGG	1 cut(s)	1288
BmsI	GCATC	7 cut(s)	418, 486, 512, 650, 943, 1451, 2211
BmtI	GCTAGC	1 cut(s)	105
BmuI	ACTGGG	1 cut(s)	1288
BpiI	GAAGAC	2 cut(s)	86, 1221
BplI	GAGNNNNNCTC	2 cut(s)	1620, 1652
BpmI	CTGGAG	1 cut(s)	1647
BpuMI	CCSGG	1 cut(s)	2488
BsaAI	YACGTR	1 cut(s)	2526
BsaBI	GATNNNNATC	2 cut(s)	222, 1740
BsaHI	GRCGYC	1 cut(s)	1288
BsaI	GGTCTC	2 cut(s)	1796, 1957
BsaXI	ACNNNNNCTCC	4 cut(s)	434, 464, 1386, 1416
Bsc4I	CCNNNNNNNGG	8 cut(s)	70, 340, 465, 637, 1279, 1582, 2308, 2444
Bse1I	ACTGG	3 cut(s)	1283, 1335, 2449
Bse21I	CCTNAGG	1 cut(s)	2478
Bse3DI	GCAATG	2 cut(s)	239, 1414
Bse8I	GATNNNNATC	2 cut(s)	222, 1740
BseBI	CCWGG	3 cut(s)	1626, 2037, 2355
BseGI	GGATG	2 cut(s)	958, 1782
BseJI	GATNNNNATC	2 cut(s)	222, 1740
BseLI	CCNNNNNNNGG	8 cut(s)	70, 340, 465, 637, 1279, 1582, 2308, 2444
BseMI	GCAATG	2 cut(s)	239, 1414
BseMII	CTCAG	8 cut(s)	786, 1172, 1653, 1761, 1831, 2009, 2270, 2492
BseNI	ACTGG	3 cut(s)	1283, 1335, 2449
BseRI	GAGGAG	5 cut(s)	323, 528, 1622, 1634, 1814
BseXI	GCAGC	6 cut(s)	57, 178, 273, 661, 855, 955
BseYI	CCCAGC	3 cut(s)	1468, 2239, 2390
BsgI	GTGCAG	1 cut(s)	738
Bsh1285I	CGRYCG	1 cut(s)	1068
BshFI	GGCC	3 cut(s)	365, 1284, 1528
BshNI	GGYRCC	1 cut(s)	1236
BsiEI	CGRYCG	1 cut(s)	1068
BsiHKAI	GWGCWC	1 cut(s)	1903
BsiHKCI	CYCGRG	2 cut(s)	40, 602
BsiSI	CCGG	1 cut(s)	2487
BslI	CCNNNNNNNGG	8 cut(s)	70, 340, 465, 637, 1279, 1582, 2308, 2444
BsmAI	GTCTC	6 cut(s)	467, 470, 1114, 1796, 1957, 2259
BsnI	GGCC	3 cut(s)	365, 1284, 1528
Bso31I	GGTCTC	2 cut(s)	1796, 1957
BsoBI	CYCGRG	2 cut(s)	40, 602
Bsp1286I	GDGCHC	1 cut(s)	1903
Bsp1407I	TGTACA	1 cut(s)	1762
BspACI	CCGC	9 cut(s)	14, 17, 30, 826, 865, 1382, 2302, 2395, 2438
BspANI	GGCC	3 cut(s)	365, 1284, 1528
BspCNI	CTCAG	8 cut(s)	787, 1171, 1652, 1760, 1830, 2010, 2269, 2491
BspLI	GGNNCC	5 cut(s)	111, 1238, 1283, 1575, 1623
BspMAI	CTGCAG	1 cut(s)	936
BspMI	ACCTGC	3 cut(s)	1206, 1516, 2299
BspOI	GCTAGC	1 cut(s)	105
BspPI	GGATC	4 cut(s)	218, 544, 1176, 2520
BspQI	GCTCTTC	2 cut(s)	368, 1908
BspT107I	GGYRCC	1 cut(s)	1236
BspTI	CTTAAG	1 cut(s)	1988
BspTNI	GGTCTC	2 cut(s)	1796, 1957
BsrBI	CCGCTC	1 cut(s)	826
BsrDI	GCAATG	2 cut(s)	239, 1414
BsrGI	TGTACA	1 cut(s)	1762
BsrI	ACTGG	3 cut(s)	1283, 1335, 2449
BssNI	GRCGYC	1 cut(s)	1288
Bst2UI	CCWGG	3 cut(s)	1626, 2037, 2355
Bst4CI	ACNGT	3 cut(s)	858, 1133, 2143
Bst6I	CTCTTC	8 cut(s)	270, 368, 831, 906, 1611, 1671, 1908, 2137
BstACI	GRCGYC	1 cut(s)	1288
BstAFI	CTTAAG	1 cut(s)	1988
BstAUI	TGTACA	1 cut(s)	1762
BstBAI	YACGTR	1 cut(s)	2526
BstEII	GGTNACC	1 cut(s)	2043
BstENI	CCTNNNNNAGG	1 cut(s)	635
BstF5I	GGATG	2 cut(s)	958, 1782
BstH2I	RGCGCY	1 cut(s)	665
BstHHI	GCGC	2 cut(s)	664, 1942
BstMAI	GTCTC	6 cut(s)	467, 470, 1114, 1796, 1957, 2259
BstMCI	CGRYCG	1 cut(s)	1068
BstMWI	GCNNNNNNNGC	6 cut(s)	725, 940, 1474, 1867, 2199, 2492
BstNI	CCWGG	3 cut(s)	1626, 2037, 2355
BstNSI	RCATGY	1 cut(s)	1077
BstPI	GGTNACC	1 cut(s)	2043
BstSCI	CCNGG	4 cut(s)	1624, 2035, 2353, 2486
BstSFI	CTRYAG	3 cut(s)	932, 1501, 2116
BstV1I	GCAGC	6 cut(s)	57, 178, 273, 661, 855, 955
BstV2I	GAAGAC	2 cut(s)	86, 1221
BstX2I	RGATCY	2 cut(s)	223, 549
BstYI	RGATCY	2 cut(s)	223, 549
Bsu36I	CCTNAGG	1 cut(s)	2478
BsuRI	GGCC	3 cut(s)	365, 1284, 1528
BtgZI	GCGATG	1 cut(s)	1617
BtrI	CACGTC	2 cut(s)	6, 724
BtsCI	GGATG	2 cut(s)	958, 1782
BtsIMutI	CAGTG	3 cut(s)	711, 1138, 1276
BveI	ACCTGC	3 cut(s)	1206, 1516, 2299
CaiI	CAGNNNCTG	1 cut(s)	506
CfoI	GCGC	2 cut(s)	664, 1942
Cfr13I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
CseI	GACGC	1 cut(s)	887
Csp6I	GTAC	6 cut(s)	187, 416, 534, 1730, 1763, 1983
CspCI	CAANNNNNGTGG	4 cut(s)	2274, 2309, 2484, 2519
CviAII	CATG	9 cut(s)	554, 1074, 1352, 1419, 1541, 1554, 1943, 2027, 2190
CviQI	GTAC	6 cut(s)	187, 416, 534, 1730, 1763, 1983
DraI	TTTAAA	1 cut(s)	1365
Eam1104I	CTCTTC	8 cut(s)	270, 368, 831, 906, 1611, 1671, 1908, 2137
EarI	CTCTTC	8 cut(s)	270, 368, 831, 906, 1611, 1671, 1908, 2137
EciI	GGCGGA	2 cut(s)	45, 2410
Eco31I	GGTCTC	2 cut(s)	1796, 1957
Eco32I	GATATC	2 cut(s)	2058, 2283
Eco47I	GGWCC	3 cut(s)	109, 1550, 1573
Eco47III	AGCGCT	1 cut(s)	663
Eco57I	CTGAAG	1 cut(s)	1889
Eco81I	CCTNAGG	1 cut(s)	2478
Eco88I	CYCGRG	2 cut(s)	40, 602
Eco91I	GGTNACC	1 cut(s)	2043
EcoNI	CCTNNNNNAGG	1 cut(s)	635
EcoO109I	RGGNCCY	1 cut(s)	1573
EcoO65I	GGTNACC	1 cut(s)	2043
EcoRII	CCWGG	3 cut(s)	1624, 2035, 2353
EcoRV	GATATC	2 cut(s)	2058, 2283
FaeI	CATG	9 cut(s)	557, 1077, 1355, 1422, 1544, 1557, 1946, 2030, 2193
FalI	AAGNNNNNCTT	2 cut(s)	2082, 2114
FatI	CATG	9 cut(s)	553, 1073, 1351, 1418, 1540, 1553, 1942, 2026, 2189
FblI	GTMKAC	1 cut(s)	1504
FokI	GGATG	2 cut(s)	965, 1789
FspBI	CTAG	2 cut(s)	102, 1346
GlaI	GCGC	2 cut(s)	663, 1941
GsaI	CCCAGC	3 cut(s)	1472, 2243, 2394
GsuI	CTGGAG	1 cut(s)	1647
HaeII	RGCGCY	1 cut(s)	665
HaeIII	GGCC	3 cut(s)	365, 1284, 1528
HapII	CCGG	1 cut(s)	2487
HgaI	GACGC	1 cut(s)	887
HhaI	GCGC	2 cut(s)	664, 1942
Hin1I	GRCGYC	1 cut(s)	1288
Hin1II	CATG	9 cut(s)	557, 1077, 1355, 1422, 1544, 1557, 1946, 2030, 2193
Hin6I	GCGC	2 cut(s)	662, 1940
HinP1I	GCGC	2 cut(s)	662, 1940
HincII	GTYRAC	2 cut(s)	1597, 1771
HindII	GTYRAC	2 cut(s)	1597, 1771
HindIII	AAGCTT	3 cut(s)	299, 1669, 2452
HinfI	GANTC	8 cut(s)	449, 455, 679, 878, 914, 1115, 2153, 2366
HpaII	CCGG	1 cut(s)	2487
HphI	GGTGA	5 cut(s)	85, 1367, 2037, 2323, 2466
Hpy166II	GTNNAC	6 cut(s)	241, 250, 1505, 1597, 1763, 1771
Hpy188III	TCNNGA	6 cut(s)	328, 576, 602, 902, 977, 2422
Hpy8I	GTNNAC	6 cut(s)	241, 250, 1505, 1597, 1763, 1771
Hpy99I	CGWCG	4 cut(s)	29, 903, 978, 1290
HpyAV	CCTTC	5 cut(s)	631, 727, 1564, 2485, 2501
HpyCH4III	ACNGT	3 cut(s)	858, 1133, 2143
HpyCH4IV	ACGT	7 cut(s)	5, 723, 820, 1288, 1593, 1897, 2525
HpyF10VI	GCNNNNNNNGC	6 cut(s)	725, 940, 1474, 1867, 2199, 2492
HpySE526I	ACGT	7 cut(s)	5, 723, 820, 1288, 1593, 1897, 2525
Hsp92I	GRCGYC	1 cut(s)	1288
Hsp92II	CATG	9 cut(s)	557, 1077, 1355, 1422, 1544, 1557, 1946, 2030, 2193
HspAI	GCGC	2 cut(s)	662, 1940
LguI	GCTCTTC	2 cut(s)	368, 1908
LmnI	GCTCC	2 cut(s)	1621, 2397
Lsp1109I	GCAGC	6 cut(s)	57, 178, 273, 661, 855, 955
LweI	GCATC	7 cut(s)	418, 486, 512, 650, 943, 1451, 2211
MaeI	CTAG	2 cut(s)	102, 1346
MaeII	ACGT	7 cut(s)	5, 723, 820, 1288, 1593, 1897, 2525
MaeIII	GTNAC	7 cut(s)	355, 1153, 1218, 2043, 2137, 2214, 2363
MbiI	CCGCTC	1 cut(s)	826
MfeI	CAATTG	2 cut(s)	1104, 2103
MflI	RGATCY	2 cut(s)	223, 549
MhlI	GDGCHC	1 cut(s)	1903
MlyI	GAGTC	5 cut(s)	458, 464, 887, 923, 2360
MmeI	TCCRAC	3 cut(s)	420, 648, 1528
MroXI	GAANNNNTTC	1 cut(s)	678
MslI	CAYNNNNRTG	1 cut(s)	1356
MspA1I	CMGCKG	2 cut(s)	2243, 2304
MspCI	CTTAAG	1 cut(s)	1988
MspI	CCGG	1 cut(s)	2487
MspR9I	CCNGG	4 cut(s)	1626, 2037, 2355, 2488
MunI	CAATTG	2 cut(s)	1104, 2103
MvaI	CCWGG	3 cut(s)	1626, 2037, 2355
MwoI	GCNNNNNNNGC	6 cut(s)	725, 940, 1474, 1867, 2199, 2492
NciI	CCSGG	1 cut(s)	2488
NheI	GCTAGC	1 cut(s)	101
NlaIII	CATG	9 cut(s)	557, 1077, 1355, 1422, 1544, 1557, 1946, 2030, 2193
NlaIV	GGNNCC	5 cut(s)	111, 1238, 1283, 1575, 1623
NmuCI	GTSAC	5 cut(s)	355, 1218, 2043, 2137, 2363
NspI	RCATGY	1 cut(s)	1077
PaeI	GCATGC	1 cut(s)	1077
PaqCI	CACCTGC	1 cut(s)	2299
PciSI	GCTCTTC	2 cut(s)	368, 1908
PcsI	WCGNNNNNNNCGW	1 cut(s)	1599
PdmI	GAANNNNTTC	1 cut(s)	678
PfeI	GAWTC	3 cut(s)	679, 1115, 2153
PflFI	GACNNNGTC	2 cut(s)	323, 1949
Ple19I	CGATCG	1 cut(s)	1068
PleI	GAGTC	5 cut(s)	457, 463, 886, 922, 2360
PpsI	GAGTC	5 cut(s)	457, 463, 886, 922, 2360
Ppu21I	YACGTR	1 cut(s)	2526
PpuMI	RGGWCCY	1 cut(s)	1573
Psp5II	RGGWCCY	1 cut(s)	1573
Psp6I	CCWGG	3 cut(s)	1624, 2035, 2353
PspEI	GGTNACC	1 cut(s)	2043
PspFI	CCCAGC	3 cut(s)	1468, 2239, 2390
PspGI	CCWGG	3 cut(s)	1624, 2035, 2353
PspN4I	GGNNCC	5 cut(s)	111, 1238, 1283, 1575, 1623
PspPI	GGNCC	4 cut(s)	109, 1282, 1550, 1573
PspPPI	RGGWCCY	1 cut(s)	1573
PstI	CTGCAG	1 cut(s)	936
PstNI	CAGNNNCTG	1 cut(s)	506
PsuI	RGATCY	2 cut(s)	223, 549
PsyI	GACNNNGTC	2 cut(s)	323, 1949
PvuI	CGATCG	1 cut(s)	1068
PvuII	CAGCTG	1 cut(s)	2243
RsaI	GTAC	6 cut(s)	188, 417, 535, 1731, 1764, 1984
RsaNI	GTAC	6 cut(s)	187, 416, 534, 1730, 1763, 1983
RseI	CAYNNNNRTG	1 cut(s)	1356
SapI	GCTCTTC	2 cut(s)	368, 1908
Sau96I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
SchI	GAGTC	5 cut(s)	458, 464, 887, 923, 2360
ScrFI	CCNGG	4 cut(s)	1626, 2037, 2355, 2488
SduI	GDGCHC	1 cut(s)	1903
SfaNI	GCATC	7 cut(s)	418, 486, 512, 650, 943, 1451, 2211
SfcI	CTRYAG	3 cut(s)	932, 1501, 2116
SinI	GGWCC	3 cut(s)	109, 1550, 1573
SmiMI	CAYNNNNRTG	1 cut(s)	1356
SmlI	CTYRAG	1 cut(s)	1988
SmoI	CTYRAG	1 cut(s)	1988
SphI	GCATGC	1 cut(s)	1077
SsiI	CCGC	9 cut(s)	14, 17, 30, 826, 865, 1382, 2302, 2395, 2438
SspI	AATATT	2 cut(s)	526, 1877
SspMI	CTAG	2 cut(s)	102, 1346
StyD4I	CCNGG	4 cut(s)	1624, 2035, 2353, 2486
TaaI	ACNGT	3 cut(s)	858, 1133, 2143
TaiI	ACGT	7 cut(s)	8, 726, 823, 1291, 1596, 1900, 2528
TaqI	TCGA	8 cut(s)	24, 254, 273, 541, 976, 1864, 2249, 2423
TatI	WGTACW	1 cut(s)	1762
TauI	GCSGC	5 cut(s)	17, 829, 868, 2304, 2440
TfiI	GAWTC	3 cut(s)	679, 1115, 2153
TscAI	CASTG	3 cut(s)	711, 1138, 1283
TseFI	GTSAC	5 cut(s)	355, 1218, 2043, 2137, 2363
TseI	GCWGC	6 cut(s)	45, 166, 286, 649, 868, 943
Tsp45I	GTSAC	5 cut(s)	355, 1218, 2043, 2137, 2363
TspDTI	ATGAA	8 cut(s)	650, 906, 1170, 1340, 1716, 1722, 2015, 2481
TspGWI	ACGGA	3 cut(s)	1385, 2125, 2167
TspRI	CASTG	3 cut(s)	711, 1138, 1283
Tth111I	GACNNNGTC	2 cut(s)	323, 1949
Vha464I	CTTAAG	1 cut(s)	1988
VpaK11BI	GGWCC	3 cut(s)	109, 1550, 1573
XagI	CCTNNNNNAGG	1 cut(s)	635
XapI	RAATTY	5 cut(s)	1847, 1885, 2287, 2322, 2470
XceI	RCATGY	1 cut(s)	1077
XmiI	GTMKAC	1 cut(s)	1504
XmnI	GAANNNNTTC	1 cut(s)	678
XspI	CTAG	2 cut(s)	102, 1346
ZraI	GACGTC	1 cut(s)	1289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.