Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006301

Physical Location & Seq

Genomic Coordinates Forward (+)

24557086 .. 24562934

Gene Structure 5849 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g24210.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGATGTGTGAAGTGGCGGTAATCGACGGCGGAGATGACCCGAGCAGCCAGAAATCTACCAACCAAGATCATGTGAGGAAGACCCAGAAGCGAAAGAGGGCTAGTTTGGACCCAGAATGTTTGGGAGTCGAGGCGAAAAGGGCGAAGATTGAATCTTTTCGCAAGCAGCTTGATGGGTTGTTTGGGTACTACAAGGAAGTGATGGATCAGAAATTAGATTTGGATCCGAAGCAGTATGGGAACAATGTGAATTCGGTCATTGGGGCTTTGATCGAAGAGAGTGGCCTGCCATTGTCCAAGCTTGTTGAGGAGGTCTTCGACAAGGTCAAGAACGGCAATGAGGTTCTTGGGAATGTGACTTTGGCCTATGTGAAGAGCATTGTGCTCTTTGTTGGGCAAAGAGTGATGTACGGCGTGCCAAATGTTGATGCTGATGTGTTGGAGGACGAGTCCGAGTCCTGTCTTTGGTGTTGGGAGACAAGAGATGTAAAGTTGATGCCAGCATCTGTGCGAGGAGTATTGAATATTAGGCGTACTTGTCGAAGAAAAATCCATGAGAGGGTTACTGCTGTTTCTGCAATGACAATGGCGCTACAAAATTCCGAGAGCGATCAAAATTACATACATGACCTGACGAAGGCATCAGAACAGCTTGATAAAGCGCTGTCTGAGGAAAAGATTCGATCGTTAATCGATCGCTTATCAGTGAAAAATGGTGCAGACATGGCAAAGAAGGAAGCAAAAAGAGAAGAAAAACTACTAATGAAACAAATGGAGAGAGATAAACGAGAGGCTGAGAAAGAGAAAAAAAAATTGGAACGTGAGCGTCAAAAGGAAGAGTTGCAAAGTGAGAAAGAGTTAAAGCGGCTGCAAGGGGAGTCAGAAAAAGATGGAAAGCGTCGTGAAAAAGAAGAGTCTGAAATGAGGAAACTGCAGAGGAAGCAGCAAGAAGATGCTGAAAGAGAACAACGCCATCGAGAAAAGGAAGAAGCTGAATTGAAAAAAAAACTTTCCATAAAAAAACAAGCTTCAATTATGGAACGGTTTGTCAAAAGAAGTAAAACCAGCTCAGCGTGCCAGAATGACCAGTTCCCAACTAAAACAATTGTCTCTGAATCATTGAGTAAAAACAGTGAAAATATGCCTGATGTAGTCACTCAGTCAATGGACAGCACTCTTTCATCTAATGTAGAGATTAATGCCGAGGATGTACGCAGGTCACACTTGTCTTCTTGGCGTCACATTGGTCATTATATTTGTTCAAACAGAAACCAACACTGGGGCCGACGTCAGAAGCCTAAGACTGAACTAGTTAAGGAACTCAAGCTTACAACCAGTAAAGAGCTAGTTCATGTGGACGATTTAAGCACTGAGAAACTAGCTGATAGATGGGGAGAACAAGTTTCTAATGATAAATCATGTCAGATTAATACAGATTGTTCTCTTGCTGCTGTTAAAATATGCAAGCGGGGAAAACAGCTTTTACAATTTGATAAAAGCTGTAGACCTGCATTTTATGGTATATGGCCTAAGAAAAGTCGTGTTGTTGGACCTTGCCACCCTTTTAGGAGGGACCCAGACTTGGATTATGATGTTGATAGCGATGAGGAGTGGGAAGAGGAGGAGCCTGGTGAAAACCTCTCAGATTGTGATAAAGACGATGAAGAAAGCTTGGAAGAGGGGTGTTCAAAACCTGATGATGAAGATGAAGGTGAAGATGGCTTTCTTGTACCTGACGGGTATCTCTCGGAAAATGAGGGTGTACAAGTGGACAGGATGGAAACTGATACAACATTTGAGGAAACCAGAAGCTCACCCAGAATTAAGCAAGATTTAGAGAGTGAAAAATTCTCTATATTGCTTCGACAGCAAAAATATTTAGGAAATTTGACAGAACGTGCTCTTCAGAAAAATCAGCCTTTAATTATATCAAATTTGGCGCATGACAAGGTCTCTTTGGTAAAGGCTGAAGATCTTAATGGTACTCTTAAGCTGGAACAGATGTGCTTGCAAGCTCTGAGTATGCATGTATTTCCTGGTCACTCACATGTGGAGATATCAGTTGATGGCATACAAGAAGACAACCAAGAAGTTTGTCTTTTAAGTGGCAGTCGCTGTATTAAATCAACCTCTGCTGTGACAGCTATACCAGAATCAGATTTGCCTACAATCGTTTCTGTCATTCAATCATGCTCACAGAGCATCAATAAGGTGTTACAAACTTTGCAACACAAGTTCCCAGCTGCATCAAAGTCTCAGTTAAGGAATAAAGTGCGTGAGATATCGGATTTTGTGGATAACCGCTGGCAGGTGAAGAAAGAAATTTTGGAGAAAGTTGGCTTGTCAATATCTCCTGAAAAGAGTGCTGGGCGGAGCAAGAGTATAGCTATGTTTTTCTCGAAAAGGTGCTTGCCGCCTGCTGGTAAAAGCTTTAATCCTAATGAAAATTCACCTCAGCTAGCTGGGAAGGCAAGTTCTGCAGAAGGGCATCAGAGTTGCACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006338 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008283 GO:0009555 GO:0009888 GO:0009934 GO:0009987 GO:0010026 GO:0016043 GO:0022603 GO:0022607 GO:0030154 GO:0031497 GO:0031507 GO:0032501 GO:0032502 GO:0032991 GO:0033186 GO:0033554 GO:0034622 GO:0034641 GO:0034728 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0045787 GO:0046483 GO:0048229 GO:0048366 GO:0048367 GO:0048509 GO:0048518 GO:0048522 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0051726 GO:0065003 GO:0065004 GO:0065007 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0090304 GO:0090558 GO:0099402 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CAF1A

Protein Analysis

835

Amino Acids

94.86

Weight (kDa)

6.05

Isoelectric Point (pI)

59.06

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65470 AT1G65470
fragaria_vesca	FvH4_6g00081 FvH4_6g00081 FvH4_6g00081 FvH4_6g00081 FvH4_6g00081 FvH4_6g00081
malus_domestica	MD00G1017400.v1.1 MD12G1261700.v1.1
prunus_persica	Prupe.6G365000_v2.0.a1 Prupe.6G365000_v2.0.a1
pyrus_communis	pycom04g21970 pycom12g24210
rosa_chinensis	RchiOBHm_Chr3g0447421
rosa_laevigata	RLG00000026000
rosa_multiflora	Rmu_ssc0000473.1_g000021
rosa_roxburghii	Rroxscaffold_6G00425620
rosa_rugosa	Rorug02G0601300 Rorug02G0601400 Rorug02G0601500 Rorug02G0601600
rosa_samantha	Rh3AG001500 Rh3BG001200 Rh3CG001200 Rh3DG001400
rosa_wichuraiana	Rw3G000090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2299
AatII	GACGTC	1 cut(s)	1291
Acc36I	ACCTGC	3 cut(s)	1206, 1516, 2299
AccI	GTMKAC	1 cut(s)	1504
AciI	CCGC	7 cut(s)	17, 30, 865, 1468, 2302, 2371, 2414
AclWI	GGATC	3 cut(s)	213, 218, 231
AcsI	RAATTY	7 cut(s)	250, 598, 1847, 1885, 1933, 2322, 2446
AcuI	CTGAAG	2 cut(s)	1889, 1989
AcyI	GRCGYC	2 cut(s)	1237, 1288
AfaI	GTAC	7 cut(s)	188, 410, 535, 1212, 1731, 1764, 1984
AfeI	AGCGCT	1 cut(s)	663
AfiI	CCNNNNNNNGG	7 cut(s)	465, 559, 637, 1279, 1582, 2308, 2420
AflII	CTTAAG	1 cut(s)	1988
AflIII	ACRYGT	1 cut(s)	2047
AgsI	TTSAA	7 cut(s)	152, 523, 1000, 1032, 1263, 1689, 2186
AhlI	ACTAGT	1 cut(s)	1309
AjnI	CCWGG	2 cut(s)	1627, 2035
AjuI	GAANNNNNNNTTGG	8 cut(s)	203, 235, 344, 376, 797, 829, 2309, 2341
AloI	GAACNNNNNNTCC	2 cut(s)	2458, 2490
Alw21I	GWGCWC	2 cut(s)	387, 1903
Alw26I	GTCTC	4 cut(s)	470, 1114, 1957, 2259
AlwI	GGATC	3 cut(s)	213, 218, 231
AlwNI	CAGNNNCTG	2 cut(s)	506, 2115
Ama87I	CYCGRG	1 cut(s)	40
Aor51HI	AGCGCT	1 cut(s)	663
AoxI	GGCC	4 cut(s)	283, 363, 1282, 1526
ApeKI	GCWGC	6 cut(s)	45, 166, 868, 943, 1448, 2243
ApoI	RAATTY	7 cut(s)	250, 598, 1847, 1885, 1933, 2322, 2446
AseI	ATTAAT	2 cut(s)	1197, 1428
Asp700I	GAANNNNTTC	2 cut(s)	156, 678
AspLEI	GCGC	3 cut(s)	592, 664, 1942
AspS9I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
AsuHPI	GGTGA	5 cut(s)	1643, 1724, 1806, 2323, 2442
AsuNHI	GCTAGC	1 cut(s)	2458
AvaI	CYCGRG	1 cut(s)	40
AvaII	GGWCC	3 cut(s)	109, 1550, 1573
BamHI	GGATCC	1 cut(s)	223
BarI	GAAGNNNNNNTAC	2 cut(s)	741, 773
BbsI	GAAGAC	4 cut(s)	86, 307, 1221, 2085
Bbv12I	GWGCWC	2 cut(s)	387, 1903
BbvCI	CCTCAGC	1 cut(s)	2454
BbvI	GCAGC	6 cut(s)	57, 178, 855, 955, 1435, 2230
BccI	CCATC	8 cut(s)	167, 196, 884, 981, 1383, 1712, 1771, 2060
BceAI	ACGGC	3 cut(s)	43, 349, 427
BciT130I	CCWGG	2 cut(s)	1629, 2037
BcoDI	GTCTC	4 cut(s)	470, 1114, 1957, 2259
BcuI	ACTAGT	1 cut(s)	1309
BfaI	CTAG	5 cut(s)	102, 1310, 1346, 1379, 2459
BfmI	CTRYAG	3 cut(s)	932, 1501, 2478
BfoI	RGCGCY	2 cut(s)	593, 665
BfrI	CTTAAG	1 cut(s)	1988
BfuAI	ACCTGC	3 cut(s)	1206, 1516, 2299
BglII	AGATCT	1 cut(s)	1972
BisI	GCNGC	8 cut(s)	46, 167, 866, 869, 944, 1449, 2244, 2414
BlpI	GCTNAGC	1 cut(s)	1069
BlsI	GCNGC	8 cut(s)	47, 168, 867, 870, 945, 1450, 2245, 2415
Bme1390I	CCNGG	2 cut(s)	1629, 2037
Bme18I	GGWCC	3 cut(s)	109, 1550, 1573
BmeT110I	CYCGRG	1 cut(s)	40
BmgT120I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
BmiI	GGNNCC	6 cut(s)	111, 225, 1283, 1574, 1575, 1626
BmrFI	CCNGG	2 cut(s)	1629, 2037
BmrI	ACTGGG	1 cut(s)	1288
BmsI	GCATC	8 cut(s)	418, 486, 512, 650, 943, 2211, 2255, 2497
BmtI	GCTAGC	1 cut(s)	2462
BmuI	ACTGGG	1 cut(s)	1288
BpiI	GAAGAC	4 cut(s)	86, 307, 1221, 2085
Bpu10I	CCTNAGC	1 cut(s)	2454
Bpu1102I	GCTNAGC	1 cut(s)	1069
BpuEI	CTTGAG	1 cut(s)	1307
Bsa29I	ATCGAT	1 cut(s)	693
BsaAI	YACGTR	1 cut(s)	2502
BsaBI	GATNNNNATC	1 cut(s)	222
BsaHI	GRCGYC	2 cut(s)	1237, 1288
BsaI	GGTCTC	1 cut(s)	1957
BsaJI	CCNNGG	1 cut(s)	1203
BsaXI	ACNNNNNCTCC	6 cut(s)	434, 464, 1386, 1416, 2365, 2395
Bsc4I	CCNNNNNNNGG	7 cut(s)	465, 559, 637, 1279, 1582, 2308, 2420
Bse1I	ACTGG	3 cut(s)	1087, 1283, 1335
Bse3DI	GCAATG	2 cut(s)	343, 585
Bse8I	GATNNNNATC	1 cut(s)	222
BseBI	CCWGG	2 cut(s)	1629, 2037
BseCI	ATCGAT	1 cut(s)	693
BseDI	CCNNGG	1 cut(s)	1203
BseGI	GGATG	2 cut(s)	1213, 1782
BseJI	GATNNNNATC	1 cut(s)	222
BseLI	CCNNNNNNNGG	7 cut(s)	465, 559, 637, 1279, 1582, 2308, 2420
BseMI	GCAATG	2 cut(s)	343, 585
BseMII	CTCAG	9 cut(s)	660, 786, 1083, 1172, 1362, 1656, 2009, 2270, 2468
BseNI	ACTGG	3 cut(s)	1087, 1283, 1335
BseRI	GAGGAG	5 cut(s)	323, 528, 1622, 1634, 1637
BseXI	GCAGC	6 cut(s)	57, 178, 855, 955, 1435, 2230
BseYI	CCCAGC	3 cut(s)	2239, 2366, 2462
BsgI	GTGCAG	1 cut(s)	738
Bsh1285I	CGRYCG	2 cut(s)	686, 697
BshFI	GGCC	4 cut(s)	285, 365, 1284, 1528
BshVI	ATCGAT	1 cut(s)	693
BsiEI	CGRYCG	2 cut(s)	686, 697
BsiHKAI	GWGCWC	2 cut(s)	387, 1903
BsiHKCI	CYCGRG	1 cut(s)	40
BslFI	GGGAC	1 cut(s)	1586
BslI	CCNNNNNNNGG	7 cut(s)	465, 559, 637, 1279, 1582, 2308, 2420
BsmAI	GTCTC	4 cut(s)	470, 1114, 1957, 2259
BsmFI	GGGAC	1 cut(s)	1586
BsnI	GGCC	4 cut(s)	285, 365, 1284, 1528
Bso31I	GGTCTC	1 cut(s)	1957
BsoBI	CYCGRG	1 cut(s)	40
Bsp1286I	GDGCHC	2 cut(s)	387, 1903
Bsp1407I	TGTACA	1 cut(s)	1762
Bsp143I	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
Bsp1720I	GCTNAGC	1 cut(s)	1069
BspACI	CCGC	7 cut(s)	17, 30, 865, 1468, 2302, 2371, 2414
BspANI	GGCC	4 cut(s)	285, 365, 1284, 1528
BspCNI	CTCAG	9 cut(s)	661, 787, 1082, 1171, 1363, 1655, 2010, 2269, 2467
BspDI	ATCGAT	1 cut(s)	693
BspLI	GGNNCC	6 cut(s)	111, 225, 1283, 1574, 1575, 1626
BspMAI	CTGCAG	2 cut(s)	936, 2482
BspMI	ACCTGC	3 cut(s)	1206, 1516, 2299
BspOI	GCTAGC	1 cut(s)	2462
BspPI	GGATC	3 cut(s)	213, 218, 231
BspQI	GCTCTTC	2 cut(s)	368, 1908
BspTI	CTTAAG	1 cut(s)	1988
BspTNI	GGTCTC	1 cut(s)	1957
BsrDI	GCAATG	2 cut(s)	343, 585
BsrGI	TGTACA	1 cut(s)	1762
BsrI	ACTGG	3 cut(s)	1087, 1283, 1335
BssECI	CCNNGG	1 cut(s)	1203
BssMI	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
BssNI	GRCGYC	2 cut(s)	1237, 1288
Bst2UI	CCWGG	2 cut(s)	1629, 2037
Bst4CI	ACNGT	2 cut(s)	1044, 1133
Bst6I	CTCTTC	7 cut(s)	270, 368, 831, 906, 1611, 1671, 1908
BstACI	GRCGYC	2 cut(s)	1237, 1288
BstAFI	CTTAAG	1 cut(s)	1988
BstAPI	GCANNNNNTGC	1 cut(s)	2477
BstAUI	TGTACA	1 cut(s)	1762
BstBAI	YACGTR	1 cut(s)	2502
BstENI	CCTNNNNNAGG	1 cut(s)	635
BstF5I	GGATG	2 cut(s)	1213, 1782
BstH2I	RGCGCY	2 cut(s)	593, 665
BstHHI	GCGC	3 cut(s)	592, 664, 1942
BstKTI	GATC	8 cut(s)	70, 208, 226, 273, 613, 686, 697, 1975
BstMAI	GTCTC	4 cut(s)	470, 1114, 1957, 2259
BstMBI	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
BstMCI	CGRYCG	2 cut(s)	686, 697
BstNI	CCWGG	2 cut(s)	1629, 2037
BstNSI	RCATGY	2 cut(s)	2030, 2051
BstSCI	CCNGG	2 cut(s)	1627, 2035
BstSFI	CTRYAG	3 cut(s)	932, 1501, 2478
BstV1I	GCAGC	6 cut(s)	57, 178, 855, 955, 1435, 2230
BstV2I	GAAGAC	4 cut(s)	86, 307, 1221, 2085
BstX2I	RGATCY	2 cut(s)	223, 1972
BstYI	RGATCY	2 cut(s)	223, 1972
Bsu15I	ATCGAT	1 cut(s)	693
BsuRI	GGCC	4 cut(s)	285, 365, 1284, 1528
BsuTUI	ATCGAT	1 cut(s)	693
BtgZI	GCGATG	1 cut(s)	1617
BtsCI	GGATG	2 cut(s)	1213, 1782
BtsIMutI	CAGTG	4 cut(s)	711, 1138, 1276, 1368
BveI	ACCTGC	3 cut(s)	1206, 1516, 2299
CaiI	CAGNNNCTG	2 cut(s)	506, 2115
CfoI	GCGC	3 cut(s)	592, 664, 1942
Cfr13I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
ClaI	ATCGAT	1 cut(s)	693
CseI	GACGC	3 cut(s)	815, 887, 1226
Csp6I	GTAC	7 cut(s)	187, 409, 534, 1211, 1730, 1763, 1983
CviQI	GTAC	7 cut(s)	187, 409, 534, 1211, 1730, 1763, 1983
DpnI	GATC	8 cut(s)	69, 207, 225, 272, 612, 685, 696, 1974
DpnII	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
Eam1104I	CTCTTC	7 cut(s)	270, 368, 831, 906, 1611, 1671, 1908
EarI	CTCTTC	7 cut(s)	270, 368, 831, 906, 1611, 1671, 1908
EciI	GGCGGA	2 cut(s)	45, 2386
Eco31I	GGTCTC	1 cut(s)	1957
Eco32I	GATATC	2 cut(s)	2058, 2283
Eco47I	GGWCC	3 cut(s)	109, 1550, 1573
Eco47III	AGCGCT	1 cut(s)	663
Eco57I	CTGAAG	2 cut(s)	1889, 1989
Eco88I	CYCGRG	1 cut(s)	40
EcoNI	CCTNNNNNAGG	1 cut(s)	635
EcoO109I	RGGNCCY	1 cut(s)	1573
EcoRI	GAATTC	1 cut(s)	250
EcoRII	CCWGG	2 cut(s)	1627, 2035
EcoRV	GATATC	2 cut(s)	2058, 2283
EcoT22I	ATGCAT	1 cut(s)	2028
FalI	AAGNNNNNCTT	2 cut(s)	2082, 2114
FaqI	GGGAC	1 cut(s)	1586
FauI	CCCGC	1 cut(s)	1461
FblI	GTMKAC	1 cut(s)	1504
Fnu4HI	GCNGC	8 cut(s)	46, 167, 866, 869, 944, 1449, 2244, 2414
FokI	GGATG	2 cut(s)	1220, 1789
Fsp4HI	GCNGC	8 cut(s)	46, 167, 866, 869, 944, 1449, 2244, 2414
FspBI	CTAG	5 cut(s)	102, 1310, 1346, 1379, 2459
GlaI	GCGC	3 cut(s)	591, 663, 1941
GluI	GCNGC	8 cut(s)	46, 167, 866, 869, 944, 1449, 2244, 2414
GsaI	CCCAGC	3 cut(s)	2243, 2370, 2466
HaeII	RGCGCY	2 cut(s)	593, 665
HaeIII	GGCC	4 cut(s)	285, 365, 1284, 1528
HgaI	GACGC	3 cut(s)	815, 887, 1226
HhaI	GCGC	3 cut(s)	592, 664, 1942
Hin1I	GRCGYC	2 cut(s)	1237, 1288
Hin6I	GCGC	3 cut(s)	590, 662, 1940
HinP1I	GCGC	3 cut(s)	590, 662, 1940
HindIII	AAGCTT	5 cut(s)	299, 1026, 1325, 1669, 2428
HinfI	GANTC	9 cut(s)	126, 152, 449, 455, 679, 878, 914, 1115, 2153
HphI	GGTGA	5 cut(s)	1643, 1724, 1806, 2323, 2442
Hpy166II	GTNNAC	4 cut(s)	1357, 1505, 1763, 1771
Hpy188III	TCNNGA	5 cut(s)	328, 902, 977, 2354, 2398
Hpy8I	GTNNAC	4 cut(s)	1357, 1505, 1763, 1771
Hpy99I	CGWCG	3 cut(s)	29, 903, 1290
HpyAV	CCTTC	5 cut(s)	631, 727, 1703, 2461, 2477
HpyCH4III	ACNGT	2 cut(s)	1044, 1133
HpyCH4IV	ACGT	4 cut(s)	820, 1288, 1897, 2501
HpySE526I	ACGT	4 cut(s)	820, 1288, 1897, 2501
Hsp92I	GRCGYC	2 cut(s)	1237, 1288
HspAI	GCGC	3 cut(s)	590, 662, 1940
KflI	GGGWCCC	1 cut(s)	1573
Kzo9I	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
LguI	GCTCTTC	2 cut(s)	368, 1908
LmnI	GCTCC	2 cut(s)	1624, 2373
Lsp1109I	GCAGC	6 cut(s)	57, 178, 855, 955, 1435, 2230
LweI	GCATC	8 cut(s)	418, 486, 512, 650, 943, 2211, 2255, 2497
MaeI	CTAG	5 cut(s)	102, 1310, 1346, 1379, 2459
MaeII	ACGT	4 cut(s)	820, 1288, 1897, 2501
MaeIII	GTNAC	8 cut(s)	355, 562, 1153, 1218, 1238, 2039, 2137, 2214
MalI	GATC	8 cut(s)	69, 207, 225, 272, 612, 685, 696, 1974
MboI	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
MfeI	CAATTG	1 cut(s)	1104
MflI	RGATCY	2 cut(s)	223, 1972
MhlI	GDGCHC	2 cut(s)	387, 1903
MlyI	GAGTC	5 cut(s)	135, 458, 464, 887, 923
MmeI	TCCRAC	2 cut(s)	420, 1528
Mph1103I	ATGCAT	1 cut(s)	2028
MroXI	GAANNNNTTC	2 cut(s)	156, 678
MslI	CAYNNNNRTG	1 cut(s)	2046
MspA1I	CMGCKG	2 cut(s)	2243, 2304
MspCI	CTTAAG	1 cut(s)	1988
MspR9I	CCNGG	2 cut(s)	1629, 2037
MunI	CAATTG	1 cut(s)	1104
MvaI	CCWGG	2 cut(s)	1629, 2037
NdeII	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
NheI	GCTAGC	1 cut(s)	2458
NlaIV	GGNNCC	6 cut(s)	111, 225, 1283, 1574, 1575, 1626
NmeAIII	GCCGAG	1 cut(s)	1228
NmuCI	GTSAC	6 cut(s)	355, 1153, 1218, 1238, 2039, 2137
NsiI	ATGCAT	1 cut(s)	2028
NspI	RCATGY	2 cut(s)	2030, 2051
PaqCI	CACCTGC	1 cut(s)	2299
PciI	ACATGT	1 cut(s)	2047
PciSI	GCTCTTC	2 cut(s)	368, 1908
PdmI	GAANNNNTTC	2 cut(s)	156, 678
PfeI	GAWTC	4 cut(s)	152, 679, 1115, 2153
PflFI	GACNNNGTC	2 cut(s)	323, 1949
PkrI	GCNGC	8 cut(s)	47, 168, 867, 870, 945, 1450, 2245, 2415
Ple19I	CGATCG	2 cut(s)	686, 697
PleI	GAGTC	5 cut(s)	134, 457, 463, 886, 922
PpsI	GAGTC	5 cut(s)	134, 457, 463, 886, 922
Ppu21I	YACGTR	1 cut(s)	2502
PpuMI	RGGWCCY	1 cut(s)	1573
PscI	ACATGT	1 cut(s)	2047
PshBI	ATTAAT	2 cut(s)	1197, 1428
Psp5II	RGGWCCY	1 cut(s)	1573
Psp6I	CCWGG	2 cut(s)	1627, 2035
PspFI	CCCAGC	3 cut(s)	2239, 2366, 2462
PspGI	CCWGG	2 cut(s)	1627, 2035
PspN4I	GGNNCC	6 cut(s)	111, 225, 1283, 1574, 1575, 1626
PspPI	GGNCC	4 cut(s)	109, 1282, 1550, 1573
PspPPI	RGGWCCY	1 cut(s)	1573
PstI	CTGCAG	2 cut(s)	936, 2482
PstNI	CAGNNNCTG	2 cut(s)	506, 2115
PsuI	RGATCY	2 cut(s)	223, 1972
PsyI	GACNNNGTC	2 cut(s)	323, 1949
PvuI	CGATCG	2 cut(s)	686, 697
PvuII	CAGCTG	1 cut(s)	2243
RsaI	GTAC	7 cut(s)	188, 410, 535, 1212, 1731, 1764, 1984
RsaNI	GTAC	7 cut(s)	187, 409, 534, 1211, 1730, 1763, 1983
RseI	CAYNNNNRTG	1 cut(s)	2046
SapI	GCTCTTC	2 cut(s)	368, 1908
SatI	GCNGC	8 cut(s)	46, 167, 866, 869, 944, 1449, 2244, 2414
Sau3AI	GATC	8 cut(s)	67, 205, 223, 270, 610, 683, 694, 1972
Sau96I	GGNCC	4 cut(s)	109, 1282, 1550, 1573
SchI	GAGTC	5 cut(s)	135, 458, 464, 887, 923
ScrFI	CCNGG	2 cut(s)	1629, 2037
SduI	GDGCHC	2 cut(s)	387, 1903
SfaNI	GCATC	8 cut(s)	418, 486, 512, 650, 943, 2211, 2255, 2497
SfcI	CTRYAG	3 cut(s)	932, 1501, 2478
SinI	GGWCC	3 cut(s)	109, 1550, 1573
SmiMI	CAYNNNNRTG	1 cut(s)	2046
SmlI	CTYRAG	2 cut(s)	1322, 1988
SmoI	CTYRAG	2 cut(s)	1322, 1988
SpeI	ACTAGT	1 cut(s)	1309
SsiI	CCGC	7 cut(s)	17, 30, 865, 1468, 2302, 2371, 2414
SspI	AATATT	2 cut(s)	526, 1877
SspMI	CTAG	5 cut(s)	102, 1310, 1346, 1379, 2459
StyD4I	CCNGG	2 cut(s)	1627, 2035
TaaI	ACNGT	2 cut(s)	1044, 1133
TaiI	ACGT	4 cut(s)	823, 1291, 1900, 2504
TaqII	GACCGA	1 cut(s)	244
TatI	WGTACW	1 cut(s)	1762
TauI	GCSGC	2 cut(s)	868, 2416
TfiI	GAWTC	4 cut(s)	152, 679, 1115, 2153
TscAI	CASTG	4 cut(s)	711, 1138, 1283, 1375
TseFI	GTSAC	6 cut(s)	355, 1153, 1218, 1238, 2039, 2137
TseI	GCWGC	6 cut(s)	45, 166, 868, 943, 1448, 2243
Tsp45I	GTSAC	6 cut(s)	355, 1153, 1218, 1238, 2039, 2137
TspDTI	ATGAA	7 cut(s)	779, 1170, 1340, 1677, 1716, 1722, 2457
TspRI	CASTG	4 cut(s)	711, 1138, 1283, 1375
Tth111I	GACNNNGTC	2 cut(s)	323, 1949
Vha464I	CTTAAG	1 cut(s)	1988
VpaK11BI	GGWCC	3 cut(s)	109, 1550, 1573
VspI	ATTAAT	2 cut(s)	1197, 1428
XagI	CCTNNNNNAGG	1 cut(s)	635
XapI	RAATTY	7 cut(s)	250, 598, 1847, 1885, 1933, 2322, 2446
XceI	RCATGY	2 cut(s)	2030, 2051
XmiI	GTMKAC	1 cut(s)	1504
XmnI	GAANNNNTTC	2 cut(s)	156, 678
XspI	CTAG	5 cut(s)	102, 1310, 1346, 1379, 2459
ZraI	GACGTC	1 cut(s)	1289
Zsp2I	ATGCAT	1 cut(s)	2028

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.