MD00G1035900.v1.1

cytochrome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011592
Physical Location & Seq
Forward (+)
6383889 .. 6386464
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1035900.v1.1.491

Sequence Viewer

Length: 414 bp
ATGTCTGCAGGAATGGGTTCAAACTCTGAAGTTTATCTCTTCGATGAGGTGGCAAAGCACAACCACAAGAAAGATTGTTGGATCATAATCTCTGGAAAGGTTTATGATGTGACCCCTTTCTTGGAGGAACACCCTGGAGGTGATGAAGTCTTGCTATTAGCAGTCGAAAAAGATGCAACAGATGATTTTAATGATGTGGGGCACAGCGATTCCGCGAGGGAGCAGATGGAAAAATACTATGTAGGCAAGGTTGACACGTCCACCATACCGGCACAGCCAAATTACAAGCTTCCTCCTCAAGCAGCTGCTCCCGCTGAACAATCTTCTGGACTTGTGGTAAAACTTTTACAGTTTCTGCTACCCTTGTTGATCTTGGGAGCTGCATTTGCTCTGCAATACTACAATAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

138

Amino Acids

15.1

Weight (kDa)

4.95

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 15 - 84 2.5e-28 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 215
AciI CCGC 2 cut(s) 213, 312
AclWI GGATC 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 1 cut(s) 21
AjiI CACGTC 1 cut(s) 258
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 3 cut(s) 289, 305, 380
AluI AGCT 3 cut(s) 289, 305, 380
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 355
ApeKI GCWGC 3 cut(s) 302, 305, 380
Asp700I GAANNNNTTC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 292, 314, 367
BccI CCATC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 3 cut(s) 303, 306, 381
BlsI GCNGC 3 cut(s) 304, 307, 382
Bme1390I CCNGG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 282
BsaBI GATNNNNATC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse118I RCCGGY 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 86
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 133
BseJI GATNNNNATC 1 cut(s) 86
BseLI CCNNNNNNNGG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 204
BseXI GCAGC 3 cut(s) 292, 314, 367
Bsh1236I CGCG 1 cut(s) 215
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 2 cut(s) 81, 369
BspACI CCGC 2 cut(s) 213, 312
BspFNI CGCG 1 cut(s) 215
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 81, 369
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 44
BstFNI CGCG 1 cut(s) 215
BstKTI GATC 2 cut(s) 84, 372
BstMBI GATC 2 cut(s) 81, 369
BstMWI GCNNNNNNNGC 2 cut(s) 311, 386
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 204
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 3 cut(s) 292, 314, 367
BtrI CACGTC 1 cut(s) 258
CaiI CAGNNNCTG 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 268
CviJI RGCY 4 cut(s) 277, 289, 305, 380
CviKI_1 RGCY 4 cut(s) 277, 289, 305, 380
DpnI GATC 2 cut(s) 83, 371
DpnII GATC 2 cut(s) 81, 369
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 133
FaiI YATR 4 cut(s) 86, 105, 240, 266
FauI CCCGC 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 303, 306, 381
Fsp4HI GCNGC 3 cut(s) 303, 306, 381
GluI GCNGC 3 cut(s) 303, 306, 381
GsuI CTGGAG 1 cut(s) 156
HapII CCGG 1 cut(s) 269
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 1 cut(s) 209
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 2 cut(s) 253, 261
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 93, 327
Hpy8I GTNNAC 2 cut(s) 253, 261
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 4 cut(s) 8, 176, 383, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 386
HpySE526I ACGT 1 cut(s) 257
Kzo9I GATC 2 cut(s) 81, 369
LmnI GCTCC 3 cut(s) 220, 313, 377
LpnPI CCDG 5 cut(s) 78, 120, 147, 282, 312
Lsp1109I GCAGC 3 cut(s) 292, 314, 367
LweI GCATC 1 cut(s) 163
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 83, 371
MboI GATC 2 cut(s) 81, 369
MboII GAAGA 2 cut(s) 31, 315
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 280
MmeI TCCRAC 1 cut(s) 59
MnlI CCTC 6 cut(s) 40, 118, 131, 210, 303, 306
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 2 cut(s) 305, 314
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 311, 386
NdeII GATC 2 cut(s) 81, 369
NmuCI GTSAC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 3 cut(s) 304, 307, 382
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 305
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 3 cut(s) 303, 306, 381
Sau3AI GATC 2 cut(s) 81, 369
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 204
SetI ASST 8 cut(s) 51, 102, 142, 252, 260, 291, 307, 382
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 1 cut(s) 280
SsiI CCGC 2 cut(s) 213, 312
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 42, 165
TasI AATT 1 cut(s) 280
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 302, 305, 380
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.