MD07G1155300.v1.1

cytochrome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011592
Physical Location & Seq
Forward (+)
22568099 .. 22570602
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1155300.v1.1.491

Sequence Viewer

Length: 414 bp
ATGTCTGCAGGAATGGGTTCAAAATCTCAACTTTTTCAATACGATGAGGTGGCAAAGCACAACCACAAGAAAGATTGTTGGATCATAATCTCTGGAAAGGTTTATGATGTGACCCCTTTCTTGGAGGAACACCCTGGAGGCGATGAAGTCTTGCTATTAGCAGTCGAAAAAGATGCAACAGATGACTTTAATGATGTCGGCCACAGTGATTCCGCGAGGGAGCAGATGGAAAAATACTACGTAGGCAAGGTTGACACGTCCACCATACCGGCACAGCCAAATTACAAGCTTCCTGCTCAAGCAGCTGCTCAATCTGAACAATCTTCTGGACTTTTGGTAAAACTTTTACAGTTTCTGCTGCCCTTGTTGATCATGGGAGCTGCATTTGCTCTGCAATACTACAATAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

138

Amino Acids

15.2

Weight (kDa)

5.26

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 15 - 84 2.1e-28 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 215
AciI CCGC 1 cut(s) 213
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 2 cut(s) 21, 38
AjiI CACGTC 1 cut(s) 258
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 3 cut(s) 289, 305, 380
AluI AGCT 3 cut(s) 289, 305, 380
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 4 cut(s) 302, 305, 358, 380
Asp700I GAANNNNTTC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 292, 314, 345, 367
BccI CCATC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 369
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 4 cut(s) 303, 306, 359, 381
BlsI GCNGC 4 cut(s) 304, 307, 360, 382
Bme1390I CCNGG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse118I RCCGGY 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 86
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 133
BseJI GATNNNNATC 1 cut(s) 86
BseLI CCNNNNNNNGG 1 cut(s) 121
BseXI GCAGC 4 cut(s) 292, 314, 345, 367
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 121
BsnI GGCC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 81, 369
BspACI CCGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 201
BspFNI CGCG 1 cut(s) 215
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 81, 369
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 206, 351
BstBAI YACGTR 1 cut(s) 241
BstFNI CGCG 1 cut(s) 215
BstKTI GATC 2 cut(s) 84, 372
BstMBI GATC 2 cut(s) 81, 369
BstMWI GCNNNNNNNGC 2 cut(s) 302, 386
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 6
BstSNI TACGTA 1 cut(s) 241
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 292, 314, 345, 367
BsuRI GGCC 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 211
CaiI CAGNNNCTG 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 268
CviAII CATG 1 cut(s) 373
CviJI RGCY 5 cut(s) 201, 277, 289, 305, 380
CviKI_1 RGCY 5 cut(s) 201, 277, 289, 305, 380
DpnI GATC 2 cut(s) 83, 371
DpnII GATC 2 cut(s) 81, 369
EaeI YGGCCR 1 cut(s) 199
Eco105I TACGTA 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 133
FaeI CATG 1 cut(s) 376
FaiI YATR 4 cut(s) 86, 105, 266, 374
FatI CATG 1 cut(s) 372
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 4 cut(s) 303, 306, 359, 381
Fsp4HI GCNGC 4 cut(s) 303, 306, 359, 381
GluI GCNGC 4 cut(s) 303, 306, 359, 381
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 1 cut(s) 201
HapII CCGG 1 cut(s) 269
Hin1II CATG 1 cut(s) 376
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 1 cut(s) 209
HpaII CCGG 1 cut(s) 269
Hpy166II GTNNAC 2 cut(s) 253, 261
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 93, 327
Hpy8I GTNNAC 2 cut(s) 253, 261
HpyCH4III ACNGT 2 cut(s) 206, 351
HpyCH4IV ACGT 2 cut(s) 240, 257
HpyCH4V TGCA 4 cut(s) 8, 176, 383, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 386
HpySE526I ACGT 2 cut(s) 240, 257
Hsp92II CATG 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 2 cut(s) 81, 369
LmnI GCTCC 2 cut(s) 220, 377
LpnPI CCDG 6 cut(s) 78, 120, 147, 282, 306, 312
Lsp1109I GCAGC 4 cut(s) 292, 314, 345, 367
LweI GCATC 1 cut(s) 163
MaeII ACGT 2 cut(s) 240, 257
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 83, 371
MboI GATC 2 cut(s) 81, 369
MboII GAAGA 1 cut(s) 315
MluCI AATT 1 cut(s) 280
MmeI TCCRAC 1 cut(s) 59
MnlI CCTC 4 cut(s) 40, 118, 131, 210
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 302, 386
NdeII GATC 2 cut(s) 81, 369
NlaIII CATG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 4 cut(s) 304, 307, 360, 382
Ppu21I YACGTR 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 305
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 303, 306, 359, 381
Sau3AI GATC 2 cut(s) 81, 369
ScrFI CCNGG 1 cut(s) 135
SetI ASST 8 cut(s) 51, 102, 243, 252, 260, 291, 307, 382
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
SnaBI TACGTA 1 cut(s) 241
Sse9I AATT 1 cut(s) 280
SsiI CCGC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 206, 351
TaiI ACGT 2 cut(s) 243, 260
TaqI TCGA 1 cut(s) 165
TasI AATT 1 cut(s) 280
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 4 cut(s) 302, 305, 358, 380
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 159
TspRI CASTG 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.