MD00G1038700.v1.1

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015564
Physical Location & Seq
Forward (+)
6725423 .. 6726431
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1038700.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCAGCCACTAGATTAATGCGCACGTTTAAGCCAATGACTTTTCATTCCTCCTCTTCTTCTTCATCTTCTCCGCCATCAACGGCCCTGAAGTGTTTGAGCAAGGGCAGAAGAATAAACGGAGAGAAGGGACTTACTAGGAACGATAATGATAGTCACGAGAGGAAGCAAATGCATGCAGTAGCTGTGAAGGCGTCGGCAACCACGTTGGCAGAACCACAAGTCGAGAATCGGGGAGGATTACTCGTAGAATTGGCTTCCCTGGTCGGAAGGGCCAACCGTGCTTTGCCCATTCTACTGAGAACGGCTCTCAGACCAAAGTCTTTGAGACAGCAAGTCCAGATGTTCATTGAAAGGGGGATAATAGACTGTCGATTCTTTACATTACTTGCAGTTGCGGGATCTTTACTCGGTTCAGTACTGTGCTTTGTAGAGGGTTGCTTTATTGTTCTAGAGTCATATTTCCAGTATTTCCATGCCTTGTCACACAAGACGGATCAAGGCCATATGGTGCATCTTCTTATTGAAGCCATTGATATGTTCCTGGTAGGAACCGCCATGCTTATTTTCGCAATTTCCGTATGTTTTGTATGGCCTTGCTTTCGAATATTTTTTCTGTCTGCGCGAGCTGCATTTGATTCCGTCAGTGTGTGCCCGGCTCGCGAGAGAGATAATGTATTGATTTTAAGCTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

233

Amino Acids

25.78

Weight (kDa)

9.53

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 121 - 194 1.7e-16 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40480
malus_domestica MD00G1038700.v1.1 MD08G1038300.v1.1
prunus_persica Prupe.1G385900_v2.0.a1
pyrus_communis pycom15g02980
rosa_chinensis RchiOBHm_Chr6g0300251
rosa_laevigata RLG00000011354
rosa_multiflora Rmu_co8452471.1_g000001 Rmu_sc0001670.1_g000029
rosa_roxburghii Rroxscaffold_7G00167960
rosa_rugosa Rorug06G0294500
rosa_samantha Rh6AG406700 Rh6BG414500 Rh6CG421700 Rh6DG407300
rosa_wichuraiana Rw6G035590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 23
AccII CGCG 2 cut(s) 625, 663
AciI CCGC 3 cut(s) 74, 398, 555
AclWI GGATC 2 cut(s) 409, 504
AcuI CTGAAG 1 cut(s) 110
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 1 cut(s) 420
AgsI TTSAA 2 cut(s) 353, 527
AhdI GACNNNNNGTC 1 cut(s) 335
AjnI CCWGG 2 cut(s) 261, 543
AluBI AGCT 3 cut(s) 185, 629, 690
AluI AGCT 3 cut(s) 185, 629, 690
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 2 cut(s) 409, 504
AlwNI CAGNNNCTG 1 cut(s) 185
AoxI GGCC 4 cut(s) 84, 273, 502, 593
ApeKI GCWGC 2 cut(s) 5, 629
AseI ATTAAT 1 cut(s) 17
AspLEI GCGC 2 cut(s) 24, 625
AspS9I GGNCC 2 cut(s) 85, 273
AsuC2I CCSGG 1 cut(s) 656
AsuII TTCGAA 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 656
BauI CACGAG 1 cut(s) 158
BbvI GCAGC 2 cut(s) 17, 616
BccI CCATC 1 cut(s) 85
BceAI ACGGC 2 cut(s) 99, 321
BciT130I CCWGG 2 cut(s) 263, 545
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 322
BfaI CTAG 3 cut(s) 12, 138, 452
BisI GCNGC 2 cut(s) 6, 630
BlsI GCNGC 2 cut(s) 7, 631
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 3 cut(s) 263, 545, 656
BmeRI GACNNNNNGTC 1 cut(s) 335
BmgT120I GGNCC 2 cut(s) 85, 273
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 3 cut(s) 263, 545, 656
BmsI GCATC 1 cut(s) 523
BoxI GACNNNNGTC 1 cut(s) 319
BplI GAGNNNNNCTC 4 cut(s) 228, 260, 292, 324
Bpu14I TTCGAA 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 466
BseBI CCWGG 2 cut(s) 263, 545
BseDI CCNNGG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 290, 325
BseNI ACTGG 1 cut(s) 466
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 656
BseXI GCAGC 2 cut(s) 17, 616
Bsh1236I CGCG 2 cut(s) 625, 663
BshFI GGCC 4 cut(s) 86, 275, 504, 595
BsiSI CCGG 1 cut(s) 656
BslFI GGGAC 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 4 cut(s) 86, 275, 504, 595
Bsp119I TTCGAA 1 cut(s) 604
Bsp1286I GDGCHC 1 cut(s) 656
Bsp143I GATC 2 cut(s) 401, 496
Bsp68I TCGCGA 1 cut(s) 663
BspACI CCGC 3 cut(s) 74, 398, 555
BspANI GGCC 4 cut(s) 86, 275, 504, 595
BspCNI CTCAG 2 cut(s) 291, 324
BspFNI CGCG 2 cut(s) 625, 663
BspLI GGNNCC 1 cut(s) 553
BspPI GGATC 2 cut(s) 409, 504
BspT104I TTCGAA 1 cut(s) 604
BsrI ACTGG 1 cut(s) 466
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 2 cut(s) 401, 496
BssNI GRCGYC 1 cut(s) 194
BssSI CACGAG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 158
Bst2UI CCWGG 2 cut(s) 263, 545
Bst4CI ACNGT 3 cut(s) 281, 371, 423
Bst6I CTCTTC 1 cut(s) 61
BstACI GRCGYC 1 cut(s) 194
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 3 cut(s) 177, 627, 661
BstDEI CTNAG 2 cut(s) 299, 311
BstFNI CGCG 2 cut(s) 625, 663
BstHHI GCGC 2 cut(s) 24, 625
BstKTI GATC 2 cut(s) 404, 499
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 401, 496
BstMWI GCNNNNNNNGC 4 cut(s) 191, 281, 629, 660
BstNI CCWGG 2 cut(s) 263, 545
BstNSI RCATGY 1 cut(s) 179
BstPAI GACNNNNGTC 1 cut(s) 319
BstSCI CCNGG 3 cut(s) 261, 543, 654
BstSLI GKGCMC 1 cut(s) 656
BstUI CGCG 2 cut(s) 625, 663
BstV1I GCAGC 2 cut(s) 17, 616
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BsuRI GGCC 4 cut(s) 86, 275, 504, 595
BtsIMutI CAGTG 1 cut(s) 652
BtuMI TCGCGA 1 cut(s) 663
Cac8I GCNNGC 3 cut(s) 177, 627, 661
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 2 cut(s) 24, 625
Cfr13I GGNCC 2 cut(s) 85, 273
CseI GACGC 1 cut(s) 183
Csp6I GTAC 1 cut(s) 419
CviAII CATG 3 cut(s) 176, 476, 559
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 2 cut(s) 299, 311
DpnI GATC 2 cut(s) 403, 498
DpnII GATC 2 cut(s) 401, 496
DriI GACNNNNNGTC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 61
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 61
EciI GGCGGA 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 110
EcoRII CCWGG 2 cut(s) 261, 543
EcoT22I ATGCAT 1 cut(s) 177
FaeI CATG 3 cut(s) 179, 479, 562
FaiI YATR 9 cut(s) 177, 460, 477, 507, 509, 539, 560, 583, 592
FaqI GGGAC 1 cut(s) 144
FatI CATG 3 cut(s) 175, 475, 558
FauI CCCGC 1 cut(s) 391
FauNDI CATATG 1 cut(s) 507
Fnu4HI GCNGC 2 cut(s) 6, 630
Fsp4HI GCNGC 2 cut(s) 6, 630
FspAI RTGCGCAY 1 cut(s) 23
FspBI CTAG 3 cut(s) 12, 138, 452
FspI TGCGCA 1 cut(s) 23
GlaI GCGC 2 cut(s) 23, 624
GluI GCNGC 2 cut(s) 6, 630
HaeIII GGCC 4 cut(s) 86, 275, 504, 595
HapII CCGG 1 cut(s) 656
HgaI GACGC 1 cut(s) 183
HhaI GCGC 2 cut(s) 24, 625
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 3 cut(s) 179, 479, 562
Hin6I GCGC 2 cut(s) 22, 623
HinP1I GCGC 2 cut(s) 22, 623
HinfI GANTC 4 cut(s) 229, 375, 455, 638
HpaII CCGG 1 cut(s) 656
Hpy188I TCNGA 2 cut(s) 269, 314
Hpy188III TCNNGA 5 cut(s) 158, 226, 340, 452, 662
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 3 cut(s) 121, 184, 264
HpyCH4III ACNGT 3 cut(s) 281, 371, 423
HpyCH4IV ACGT 2 cut(s) 26, 206
HpyCH4V TGCA 5 cut(s) 175, 179, 392, 514, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 281, 629, 660
HpyF3I CTNAG 2 cut(s) 299, 311
HpySE526I ACGT 2 cut(s) 26, 206
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 3 cut(s) 179, 479, 562
HspAI GCGC 2 cut(s) 22, 623
Kzo9I GATC 2 cut(s) 401, 496
LpnPI CCDG 9 cut(s) 101, 248, 275, 353, 479, 530, 557, 669, 676
Lsp1109I GCAGC 2 cut(s) 17, 616
LweI GCATC 1 cut(s) 523
MaeI CTAG 3 cut(s) 12, 138, 452
MaeII ACGT 2 cut(s) 26, 206
MaeIII GTNAC 2 cut(s) 155, 483
MalI GATC 2 cut(s) 403, 498
MboI GATC 2 cut(s) 401, 496
MboII GAAGA 6 cut(s) 48, 51, 54, 60, 123, 509
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 1 cut(s) 656
MluCI AATT 2 cut(s) 251, 573
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 5 cut(s) 61, 64, 156, 230, 427
Mph1103I ATGCAT 1 cut(s) 177
MseI TTAA 3 cut(s) 17, 30, 686
MslI CAYNNNNRTG 1 cut(s) 536
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 3 cut(s) 263, 545, 656
MvaI CCWGG 2 cut(s) 263, 545
MvnI CGCG 2 cut(s) 625, 663
MwoI GCNNNNNNNGC 4 cut(s) 191, 281, 629, 660
NciI CCSGG 1 cut(s) 656
NdeI CATATG 1 cut(s) 507
NdeII GATC 2 cut(s) 401, 496
NlaIII CATG 3 cut(s) 179, 479, 562
NlaIV GGNNCC 1 cut(s) 553
NmuCI GTSAC 2 cut(s) 155, 483
NruI TCGCGA 1 cut(s) 663
NsbI TGCGCA 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 177
NspI RCATGY 1 cut(s) 179
NspV TTCGAA 1 cut(s) 604
PaeI GCATGC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 2 cut(s) 203, 576
PfeI GAWTC 3 cut(s) 229, 375, 638
PkrI GCNGC 2 cut(s) 7, 631
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PshAI GACNNNNGTC 1 cut(s) 319
PshBI ATTAAT 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 261, 543
PspGI CCWGG 2 cut(s) 261, 543
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 2 cut(s) 85, 273
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 401
RruI TCGCGA 1 cut(s) 663
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 536
SaqAI TTAA 3 cut(s) 17, 30, 686
SatI GCNGC 2 cut(s) 6, 630
Sau3AI GATC 2 cut(s) 401, 496
Sau96I GGNCC 2 cut(s) 85, 273
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 3 cut(s) 263, 545, 656
SduI GDGCHC 1 cut(s) 656
SetI ASST 5 cut(s) 29, 187, 209, 631, 692
SfaNI GCATC 1 cut(s) 523
SfuI TTCGAA 1 cut(s) 604
SmiMI CAYNNNNRTG 1 cut(s) 536
SphI GCATGC 1 cut(s) 179
Sse9I AATT 2 cut(s) 251, 573
SsiI CCGC 3 cut(s) 74, 398, 555
SspI AATATT 1 cut(s) 609
SspMI CTAG 3 cut(s) 12, 138, 452
StyD4I CCNGG 3 cut(s) 261, 543, 654
TaaI ACNGT 3 cut(s) 281, 371, 423
TaiI ACGT 2 cut(s) 29, 209
TaqI TCGA 3 cut(s) 225, 373, 604
TasI AATT 2 cut(s) 251, 573
TatI WGTACW 1 cut(s) 418
TfiI GAWTC 3 cut(s) 229, 375, 638
Tru1I TTAA 3 cut(s) 17, 30, 686
Tru9I TTAA 3 cut(s) 17, 30, 686
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 2 cut(s) 155, 483
TseI GCWGC 2 cut(s) 5, 629
Tsp45I GTSAC 2 cut(s) 155, 483
TspDTI ATGAA 3 cut(s) 35, 54, 337
TspGWI ACGGA 4 cut(s) 135, 509, 568, 631
TspRI CASTG 1 cut(s) 652
VspI ATTAAT 1 cut(s) 17
XbaI TCTAGA 1 cut(s) 451
XceI RCATGY 1 cut(s) 179
XspI CTAG 3 cut(s) 12, 138, 452
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.