MD08G1038300.v1.1

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015564
Physical Location & Seq
Reverse (-)
2812045 .. 2813387
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1038300.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCAGCCGCTAGATTATTGCGCACGTTGAAGCCCATGACTTTTCATTCATCCTCTTTTTCTCCATCTTCTCCGTCATCAACAGCACTGAAGTGTTCGAGCATGGCCGGAAGATTAAACGGGGGGAAGAAACTTACTAGGACTGATAATGATAGTCATCAGAGGAAGAAAAAGCATACAGTGGCTGTGAAGGTGTCGAAAACCACATTGACGGAGCCACAAGTTGAGAATCAGGGTGGACTCCTCGTAGATTTGGCTTCCCTGGTCGGAAAGGTCAACCGTGCTTTGCCCATTCTACTGAGAACGGCTCTCAAACCAAGGTCTTTGAGACAGCAAGTTCAGATGTTCATTGAAAGGGGGATAATAGATTGCCGATTCTTTACGTTACTTGCAGTTGCGGGATCTTTACTCGGTTCAGTACTATGCTTTGTAGAGTATTTCCATGCCTTGTCACACAAGACGGATCAAGGCCATATGGTGCATCTTCTTATTGAAGCCATTGATATGTTCTTGGTAGGCACCGCCATGCTTATTTTTGCAGTTGGGTTGTATGCCATGTTCGTGGGGTCAAAGGCCGCGAAAGGAAAGGGACCGCAGTTTTCCGACTCAAATTTGTTTGGTCTCTTCTACATGAAGGCACCTCCAGCATGGGTTAACATGGAATCAGTTTCGCAAGCGAAATCGAAAATAGGTCATGCAGTGGTGATGATACTGCAAGTGGGACTGCTGGACAAGTTCAGGAGCATACCTCTCGTGACGCCGCTCGATCTTGCTTGCTTTGCTGGATCTGTGCTTCTTTCCTCTGCTGGTATCTTCCTTCTCTCCAGACTCTCTACCACTAAGAGATCTGCAGATACTGACCGTGATGGAGTCAGAATTTTAGCTCAAGAGAAACCAAAAGTTTCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

304

Amino Acids

32.87

Weight (kDa)

10.11

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 121 - 246 8.9e-28 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40480
malus_domestica MD00G1038700.v1.1 MD08G1038300.v1.1
prunus_persica Prupe.1G385900_v2.0.a1
pyrus_communis pycom15g02980
rosa_chinensis RchiOBHm_Chr6g0300251
rosa_laevigata RLG00000011354
rosa_multiflora Rmu_co8452471.1_g000001 Rmu_sc0001670.1_g000029
rosa_roxburghii Rroxscaffold_7G00167960
rosa_rugosa Rorug06G0294500
rosa_samantha Rh6AG406700 Rh6BG414500 Rh6CG421700 Rh6DG407300
rosa_wichuraiana Rw6G035590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 23
AccB1I GGYRCC 2 cut(s) 518, 637
AccBSI CCGCTC 1 cut(s) 763
AccII CGCG 1 cut(s) 578
AciI CCGC 6 cut(s) 9, 398, 522, 576, 593, 761
AclWI GGATC 3 cut(s) 409, 471, 793
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 2 cut(s) 610, 876
AcuI CTGAAG 1 cut(s) 110
AcyI GRCGYC 1 cut(s) 758
AfaI GTAC 1 cut(s) 420
AgsI TTSAA 3 cut(s) 31, 353, 494
AjnI CCWGG 1 cut(s) 261
AleI CACNNNNGTG 1 cut(s) 91
AluBI AGCT 1 cut(s) 884
AluI AGCT 1 cut(s) 884
Alw26I GTCTC 2 cut(s) 322, 626
AlwI GGATC 3 cut(s) 409, 471, 793
AlwNI CAGNNNCTG 2 cut(s) 185, 857
AoxI GGCC 3 cut(s) 105, 469, 573
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 610, 876
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 2 cut(s) 715, 897
AvaII GGWCC 1 cut(s) 590
BanI GGYRCC 2 cut(s) 518, 637
BauI CACGAG 1 cut(s) 752
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 73, 860
BceAI ACGGC 1 cut(s) 321
BcgI CGANNNNNNTGC 2 cut(s) 733, 767
BciT130I CCWGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 322, 626
BfaI CTAG 3 cut(s) 12, 138, 910
BfmI CTRYAG 1 cut(s) 849
BglII AGATCT 1 cut(s) 845
BisI GCNGC 4 cut(s) 6, 9, 576, 761
BlsI GCNGC 4 cut(s) 7, 10, 577, 762
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 4 cut(s) 216, 520, 591, 639
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 490
BplI GAGNNNNNCTC 4 cut(s) 292, 324, 733, 765
BpmI CTGGAG 2 cut(s) 627, 808
BpuEI CTTGAG 1 cut(s) 870
BsaBI GATNNNNATC 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 2 cut(s) 261, 317
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 261, 317
BseGI GGATG 1 cut(s) 50
BseJI GATNNNNATC 1 cut(s) 156
BseMII CTCAG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 3 cut(s) 107, 471, 575
BshNI GGYRCC 2 cut(s) 518, 637
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 2 cut(s) 603, 735
BsmAI GTCTC 2 cut(s) 322, 626
BsmFI GGGAC 2 cut(s) 603, 735
BsnI GGCC 3 cut(s) 107, 471, 575
Bso31I GGTCTC 1 cut(s) 626
Bsp143I GATC 5 cut(s) 401, 463, 766, 785, 845
BspACI CCGC 6 cut(s) 9, 398, 522, 576, 593, 761
BspANI GGCC 3 cut(s) 107, 471, 575
BspCNI CTCAG 1 cut(s) 291
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 4 cut(s) 216, 520, 591, 639
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 3 cut(s) 409, 471, 793
BspT107I GGYRCC 2 cut(s) 518, 637
BspTNI GGTCTC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 763
BssECI CCNNGG 2 cut(s) 261, 317
BssMI GATC 5 cut(s) 401, 463, 766, 785, 845
BssNI GRCGYC 1 cut(s) 758
BssSI CACGAG 1 cut(s) 752
BssT1I CCWWGG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 752
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 3 cut(s) 181, 281, 863
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 675, 775
BstDEI CTNAG 2 cut(s) 299, 840
BstF5I GGATG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 578
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 5 cut(s) 404, 466, 769, 788, 848
BstMAI GTCTC 2 cut(s) 322, 626
BstMBI GATC 5 cut(s) 401, 463, 766, 785, 845
BstMWI GCNNNNNNNGC 2 cut(s) 644, 779
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 3 cut(s) 401, 785, 845
BstXI CCANNNNNNTGG 1 cut(s) 562
BstYI RGATCY 3 cut(s) 401, 785, 845
BsuRI GGCC 3 cut(s) 107, 471, 575
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 86, 186, 705
Cac8I GCNNGC 2 cut(s) 675, 775
CaiI CAGNNNCTG 2 cut(s) 185, 857
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 1 cut(s) 766
Csp6I GTAC 1 cut(s) 419
CviAII CATG 9 cut(s) 37, 103, 443, 526, 556, 631, 648, 658, 695
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 2 cut(s) 299, 840
DpnI GATC 5 cut(s) 403, 465, 768, 787, 847
DpnII GATC 5 cut(s) 401, 463, 766, 785, 845
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 626
Eco47I GGWCC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 9 cut(s) 40, 106, 446, 529, 559, 634, 651, 661, 698
FaqI GGGAC 2 cut(s) 603, 735
FatI CATG 9 cut(s) 36, 102, 442, 525, 555, 630, 647, 657, 694
FauI CCCGC 1 cut(s) 391
FauNDI CATATG 1 cut(s) 474
Fnu4HI GCNGC 4 cut(s) 6, 9, 576, 761
FokI GGATG 1 cut(s) 37
Fsp4HI GCNGC 4 cut(s) 6, 9, 576, 761
FspBI CTAG 3 cut(s) 12, 138, 910
FspI TGCGCA 1 cut(s) 23
GlaI GCGC 1 cut(s) 23
GluI GCNGC 4 cut(s) 6, 9, 576, 761
GsuI CTGGAG 2 cut(s) 627, 808
HaeIII GGCC 3 cut(s) 107, 471, 575
HapII CCGG 1 cut(s) 108
HgaI GACGC 1 cut(s) 766
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 758
Hin1II CATG 9 cut(s) 40, 106, 446, 529, 559, 634, 651, 661, 698
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 2 cut(s) 277, 655
HindII GTYRAC 2 cut(s) 277, 655
HinfI GANTC 7 cut(s) 229, 240, 375, 605, 662, 828, 870
HpaI GTTAAC 1 cut(s) 655
HpaII CCGG 1 cut(s) 108
HphI GGTGA 2 cut(s) 715, 897
Hpy166II GTNNAC 3 cut(s) 239, 277, 655
Hpy188I TCNGA 5 cut(s) 162, 269, 342, 604, 875
Hpy188III TCNNGA 4 cut(s) 739, 754, 825, 887
Hpy8I GTNNAC 3 cut(s) 239, 277, 655
HpyAV CCTTC 3 cut(s) 184, 628, 827
HpyCH4III ACNGT 3 cut(s) 181, 281, 863
HpyCH4IV ACGT 2 cut(s) 26, 383
HpyCH4V TGCA 6 cut(s) 392, 481, 539, 698, 715, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 644, 779
HpyF3I CTNAG 2 cut(s) 299, 840
HpySE526I ACGT 2 cut(s) 26, 383
Hsp92I GRCGYC 1 cut(s) 758
Hsp92II CATG 9 cut(s) 40, 106, 446, 529, 559, 634, 651, 661, 698
HspAI GCGC 1 cut(s) 22
KspAI GTTAAC 1 cut(s) 655
Kzo9I GATC 5 cut(s) 401, 463, 766, 785, 845
LmnI GCTCC 2 cut(s) 214, 741
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 490
MaeI CTAG 3 cut(s) 12, 138, 910
MaeII ACGT 2 cut(s) 26, 383
MaeIII GTNAC 3 cut(s) 384, 450, 754
MalI GATC 5 cut(s) 403, 465, 768, 787, 847
MbiI CCGCTC 1 cut(s) 763
MboI GATC 5 cut(s) 401, 463, 766, 785, 845
MboII GAAGA 7 cut(s) 60, 123, 139, 178, 476, 616, 805
MflI RGATCY 3 cut(s) 401, 785, 845
MluCI AATT 2 cut(s) 610, 876
MlyI GAGTC 4 cut(s) 234, 599, 822, 879
MmeI TCCRAC 2 cut(s) 247, 627
MnlI CCTC 6 cut(s) 64, 156, 254, 651, 759, 811
MseI TTAA 2 cut(s) 116, 654
MslI CAYNNNNRTG 4 cut(s) 91, 503, 524, 560
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 2 cut(s) 644, 779
NdeI CATATG 1 cut(s) 474
NdeII GATC 5 cut(s) 401, 463, 766, 785, 845
NlaIII CATG 9 cut(s) 40, 106, 446, 529, 559, 634, 651, 661, 698
NlaIV GGNNCC 4 cut(s) 216, 520, 591, 639
NmuCI GTSAC 2 cut(s) 450, 754
NsbI TGCGCA 1 cut(s) 23
OliI CACNNNNGTG 1 cut(s) 91
PfeI GAWTC 3 cut(s) 229, 375, 662
PkrI GCNGC 4 cut(s) 7, 10, 577, 762
PleI GAGTC 4 cut(s) 234, 599, 822, 878
PpsI GAGTC 4 cut(s) 234, 599, 822, 878
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 4 cut(s) 216, 520, 591, 639
PspPI GGNCC 1 cut(s) 590
PsrI GAACNNNNNNTAC 2 cut(s) 542, 574
PstI CTGCAG 1 cut(s) 853
PstNI CAGNNNCTG 2 cut(s) 185, 857
PsuI RGATCY 3 cut(s) 401, 785, 845
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
RseI CAYNNNNRTG 4 cut(s) 91, 503, 524, 560
SaqAI TTAA 2 cut(s) 116, 654
SatI GCNGC 4 cut(s) 6, 9, 576, 761
Sau3AI GATC 5 cut(s) 401, 463, 766, 785, 845
Sau96I GGNCC 1 cut(s) 590
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 4 cut(s) 234, 599, 822, 879
ScrFI CCNGG 1 cut(s) 263
SetI ASST 9 cut(s) 29, 195, 276, 323, 386, 643, 694, 751, 886
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 1 cut(s) 849
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 4 cut(s) 91, 503, 524, 560
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 2 cut(s) 610, 876
SsiI CCGC 6 cut(s) 9, 398, 522, 576, 593, 761
SspMI CTAG 3 cut(s) 12, 138, 910
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 3 cut(s) 181, 281, 863
TaiI ACGT 2 cut(s) 29, 386
TaqI TCGA 4 cut(s) 98, 197, 683, 765
TasI AATT 2 cut(s) 610, 876
TatI WGTACW 1 cut(s) 418
TauI GCSGC 3 cut(s) 11, 578, 763
TfiI GAWTC 3 cut(s) 229, 375, 662
Tru1I TTAA 2 cut(s) 116, 654
Tru9I TTAA 2 cut(s) 116, 654
TscAI CASTG 3 cut(s) 93, 186, 705
TseFI GTSAC 2 cut(s) 450, 754
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 450, 754
TspDTI ATGAA 4 cut(s) 35, 39, 337, 647
TspGWI ACGGA 3 cut(s) 63, 227, 476
TspRI CASTG 3 cut(s) 93, 186, 705
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 2 cut(s) 610, 876
XspI CTAG 3 cut(s) 12, 138, 910
ZrmI AGTACT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.