MD00G1055600.v1.1

Clp protease-related protein At4g12060

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012795
Physical Location & Seq
Reverse (-)
10392057 .. 10396042
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1055600.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCATCCACCTCTGTCGCACTCTCAGCTCTCTCAATCTCACCCTTTTCTACCTCTCAACCCCACAGAAACCCATGCGCCTTCTCTTCTCCCTCCCTTCCCTGCCACCTTCCCCCCTATAACCTGACAACATTTCTCGGCGCAAGGCTTTCTATTCGAATTCCGAATGTAAAGCACATGGCTTCCAAGCATCGGACGGCCGTCGCCACCGTCCTCTTCAGCCTCCCAACAGCGAAGCCCGACAGGATTTCTACTGGGAAAAGCCCCAAATGGTCCGCCAGAGCGATAAAGTCGTTTGCTATGGCTGAATTGGAAGCAAGGAAGCTCAAATATCCCAATACAGGGACCGAAGCACTTCTCATGGGGATTTTGGTTGAGGGAACAAGCCTAGCTGCAAAGTTTCTTAGAGCCAATGGAATCACACTCTTCAAGGTTCGAGATGAAACTGTAAATTTACTTGGAAAATCAGACATGTACTATTTTAGCCCTGAGCATCCTCCATTAACTGAACCAGCCCAGAGAGCCCTTGATTGGGCTTTTGATCAAAAACTAAAATCAGGTTTGAGAAAATGGGGAAATAACAGTAACTCATCTGCTTCTTGGGATTTGGTCTGA

Protein Analysis

205

Amino Acids

22.33

Weight (kDa)

10.01

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 91 - 186 3.3e-08 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25370
fragaria_vesca FvH4_7g00050
malus_domestica MD00G1055600.v1.1 MD02G1320000.v1.1
prunus_persica Prupe.2G002100_v2.0.a1
pyrus_communis pycom07g00100
rosa_chinensis RchiOBHm_Chr1g0313031 RchiOBHm_Chr1g0313191
rosa_laevigata RLG00000030817
rosa_multiflora Rmu_co8509777.1_g000001
rosa_roxburghii Rroxscaffold_4G00332400
rosa_rugosa RorugPtG0001300.1
rosa_samantha Rh1AG000400 Rh1AG001500 Rh1CG003500 Rh1CG006100 Rh1DG001400
rosa_wichuraiana Rw1G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 2 cut(s) 159, 451
AcuI CTGAAG 1 cut(s) 202
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 5 cut(s) 192, 341, 342, 531, 532
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 1 cut(s) 430
AluBI AGCT 3 cut(s) 29, 325, 392
AluI AGCT 3 cut(s) 29, 325, 392
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 2 cut(s) 159, 451
Asp700I GAANNNNTTC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 80, 143
AspS9I GGNCC 2 cut(s) 273, 345
AsuHPI GGTGA 1 cut(s) 33
AsuII TTCGAA 1 cut(s) 157
AvaII GGWCC 2 cut(s) 273, 345
BanII GRGCYC 1 cut(s) 526
BbvI GCAGC 1 cut(s) 379
BceAI ACGGC 2 cut(s) 185, 213
BclI TGATCA 1 cut(s) 541
BfaI CTAG 1 cut(s) 389
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme18I GGWCC 2 cut(s) 273, 345
BmgT120I GGNCC 2 cut(s) 273, 345
BmiI GGNNCC 1 cut(s) 346
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 3 cut(s) 14, 199, 502
BmuI ACTGGG 1 cut(s) 264
BoxI GACNNNNGTC 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 157
Bsc4I CCNNNNNNNGG 5 cut(s) 192, 341, 342, 531, 532
Bse1I ACTGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 5, 493
BseLI CCNNNNNNNGG 5 cut(s) 192, 341, 342, 531, 532
BseMII CTCAG 2 cut(s) 39, 480
BseNI ACTGG 1 cut(s) 259
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 379
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 200
BsiEI CGRYCG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 5 cut(s) 192, 341, 342, 531, 532
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 1 cut(s) 541
BspACI CCGC 1 cut(s) 276
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 2 cut(s) 38, 481
BspLI GGNNCC 1 cut(s) 346
BspT104I TTCGAA 1 cut(s) 157
BsrI ACTGG 1 cut(s) 259
BssMI GATC 1 cut(s) 541
Bst4CI ACNGT 3 cut(s) 211, 448, 584
Bst6I CTCTTC 3 cut(s) 91, 221, 431
BstBI TTCGAA 1 cut(s) 157
BstDEI CTNAG 3 cut(s) 25, 404, 489
BstF5I GGATG 2 cut(s) 5, 493
BstHHI GCGC 2 cut(s) 80, 143
BstKTI GATC 1 cut(s) 544
BstMBI GATC 1 cut(s) 541
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 26, 521
BstNSI RCATGY 1 cut(s) 475
BstPAI GACNNNNGTC 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 198
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 2 cut(s) 5, 493
CfoI GCGC 2 cut(s) 80, 143
Cfr13I GGNCC 2 cut(s) 273, 345
Csp6I GTAC 1 cut(s) 475
CviAII CATG 4 cut(s) 75, 178, 361, 472
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 3 cut(s) 25, 404, 489
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 198
EagI CGGCCG 1 cut(s) 198
Eam1104I CTCTTC 3 cut(s) 91, 221, 431
EarI CTCTTC 3 cut(s) 91, 221, 431
EciI GGCGGA 1 cut(s) 265
EclXI CGGCCG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 526
Eco47I GGWCC 2 cut(s) 273, 345
Eco52I CGGCCG 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 526
FaeI CATG 4 cut(s) 78, 181, 364, 475
FaiI YATR 6 cut(s) 76, 120, 179, 302, 362, 473
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 74, 177, 360, 471
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 1 cut(s) 480
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 1 cut(s) 389
GlaI GCGC 2 cut(s) 79, 142
GluI GCNGC 1 cut(s) 393
HaeIII GGCC 1 cut(s) 200
HhaI GCGC 2 cut(s) 80, 143
Hin1II CATG 4 cut(s) 78, 181, 364, 475
Hin6I GCGC 2 cut(s) 78, 141
HinP1I GCGC 2 cut(s) 78, 141
HinfI GANTC 1 cut(s) 417
HphI GGTGA 1 cut(s) 33
Hpy188I TCNGA 4 cut(s) 165, 195, 469, 614
Hpy188III TCNNGA 1 cut(s) 437
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 91, 107, 119
HpyCH4III ACNGT 3 cut(s) 211, 448, 584
HpyCH4V TGCA 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 521
HpyF3I CTNAG 3 cut(s) 25, 404, 489
Hsp92II CATG 4 cut(s) 78, 181, 364, 475
HspAI GCGC 2 cut(s) 78, 141
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 1 cut(s) 541
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 14, 199, 502
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 584
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MboII GAAGA 3 cut(s) 78, 208, 418
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 3 cut(s) 159, 308, 451
MnlI CCTC 7 cut(s) 22, 64, 103, 224, 233, 370, 507
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 1 cut(s) 503
MwoI GCNNNNNNNGC 2 cut(s) 26, 521
NdeII GATC 1 cut(s) 541
NlaIII CATG 4 cut(s) 78, 181, 364, 475
NlaIV GGNNCC 1 cut(s) 346
NmeAIII GCCGAG 1 cut(s) 117
NspI RCATGY 1 cut(s) 475
NspV TTCGAA 1 cut(s) 157
PciI ACATGT 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 417
PkrI GCNGC 1 cut(s) 394
PscI ACATGT 1 cut(s) 471
PshAI GACNNNNGTC 1 cut(s) 200
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 2 cut(s) 273, 345
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 1 cut(s) 541
Sau96I GGNCC 2 cut(s) 273, 345
SduI GDGCHC 1 cut(s) 526
SetI ASST 9 cut(s) 14, 31, 56, 111, 126, 327, 394, 435, 562
SfaNI GCATC 3 cut(s) 14, 199, 502
SfuI TTCGAA 1 cut(s) 157
SinI GGWCC 2 cut(s) 273, 345
Sse9I AATT 3 cut(s) 159, 308, 451
SsiI CCGC 1 cut(s) 276
SspMI CTAG 1 cut(s) 389
TaaI ACNGT 3 cut(s) 211, 448, 584
TaqI TCGA 2 cut(s) 157, 436
TaqII GACCGA 1 cut(s) 362
TasI AATT 3 cut(s) 159, 308, 451
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 1 cut(s) 417
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TseI GCWGC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 456
VpaK11BI GGWCC 2 cut(s) 273, 345
XapI RAATTY 2 cut(s) 159, 451
XceI RCATGY 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.